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Strain Name:

WC/ReJ KitlSl/J

Stock Number:

000693

Availability:

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General Terms and Conditions

Former Name      WC/ReJ-KitlSl/J    (Changed: 30-OCT-06 )
Genes & Alleles   Kitl;   KitlSl;   Pde6b;   Pde6brd1;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Mating SystemHeterozygote x +/+ sibling         (Female x Male)
Specieslaboratory mouse
GenerationF77 (21-JAN-08)

Appearance
dark grey with white head blaze, affected
Related Genotype: a/a KitlSl/+

black, unaffected
Related Genotype: a/a +/+

Important Note
This strain is homozygous for the retinal degeneration allele Pde6brd1. See article "Genetic Background Effects: Can Your Mice See?", JAX Notes Spring 2002, No. 485.

Strain Description
The multiple steel mutations (KitlSl) behave in a semidominant fashion and cause deficiencies in pigment cells, germ cells, and blood cells paralleling those caused by the Kit locus mutations (dominant spotting alleles). Most of the alleles at steel locus cause severe anemia in utero and death by 15 to 16 days of gestation in homozygous mutant mice. However, compounds of two steel mutants (e.g. KitlSl/KitlSl-d) are viable, black-eyed white, are usually sterile in one or both sexes, and have severe macrocytic anemia. Heterozygous steel mice have a diluted coat color with a small amount of white spotting, are viable and fertile, and may have a slight macrocytic anemia. Primordial germ cells are absent in the nonviable steel homozygotes and severely reduced in steel heterozygotes. Mast cells are virtually absent in skin and other tissues of steel mutant mice. Tumors tend to develop in germ-cell-deficient ovaries with advancing age.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

KitlSl/Kitl+

        involves: C3H
  • skin/coat/nails phenotype
  • abnormal skin pigmentation (MGI Ref ID J:3399)
    • mice have light ears
    • abnormal ear pigmentation (MGI Ref ID J:3399)
      • mice have light feet
  • belly spot (MGI Ref ID J:3399)
    • most mice have a white spot on the belly
  • diluted coat color (MGI Ref ID J:3399)
    • affected mice have overall dilution of coat color, more extreme on the belly than back
  • head blaze (MGI Ref ID J:3399)
    • very occasionally mice have a white blaze between their eyes
  • head spot (MGI Ref ID J:3399)
    • most mice have a white spot on the belly
  • pallor (MGI Ref ID J:3399)
    • some pups after birth are pale compared to littermates
  • pigmentation phenotype
  • abnormal skin pigmentation (MGI Ref ID J:3399)
    • mice have light ears
    • abnormal ear pigmentation (MGI Ref ID J:3399)
      • mice have light feet
  • belly spot (MGI Ref ID J:3399)
    • most mice have a white spot on the belly
  • diluted coat color (MGI Ref ID J:3399)
    • affected mice have overall dilution of coat color, more extreme on the belly than back
  • head blaze (MGI Ref ID J:3399)
    • very occasionally mice have a white blaze between their eyes
  • head spot (MGI Ref ID J:3399)
    • most mice have a white spot on the belly
  • hematopoietic system phenotype
  • anemia (MGI Ref ID J:3399)
    • mice display less severe anemia than homozygotes
  • decreased erythrocyte cell number (MGI Ref ID J:3399)
    • at 7-13 days of age, red blood cell counts are 20-30% lower than wild type
  • hearing/vestibular/ear phenotype
  • abnormal ear pigmentation (MGI Ref ID J:3399)
    • mice have light feet
  • touch/vibrissae phenotype
  • abnormal vibrissae morphology (MGI Ref ID J:3399)
    • mice have light whiskers
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:3399)
    • heterozygotes are viable and fertile
  • craniofacial phenotype
  • abnormal ear pigmentation (MGI Ref ID J:3399)
    • mice have light feet

KitlSl/Kitl+

        either: (involves: C3H * WC) or (involves: C3H * C57BL/6 * DBA/2J * WC)
  • hematopoietic system phenotype
  • anemia (MGI Ref ID J:6084)
    • mice are slightly anemic
  • decreased mast cell number (MGI Ref ID J:6084)
    • heterozygotes have decreased mast cell numbers in dorsal skin compared to wild type
  • immune system phenotype
  • decreased mast cell number (MGI Ref ID J:6084)
    • heterozygotes have decreased mast cell numbers in dorsal skin compared to wild type

KitlSl/Kitl+

        involves: 129/Sv * C3H
  • tumorigenesis
  • increased tumor incidence (MGI Ref ID J:50508)
    • male mice develop ~2-fold more tumors than controls
    • testicular teratoma (MGI Ref ID J:50508)
      • incidence is 6.92% compared to ~2.6% in controls
      • tumors are predominantly in left testis (71%) vs right (27%) or bilateral (2%)
      • percentage is greater in second and subsequent litters compared to first litter or in first litter of older females compared to young mothers

KitlSl/KitlSl

        involves: C3H
  • lethality-prenatal/perinatal
  • lethality throughout fetal growth and development (MGI Ref ID J:3399)
    • no presumed homozygotes are born; homozygotes begin to die at ~E15-15.5 from anemia
  • nervous system phenotype
  • abnormal brain development (MGI Ref ID J:3399)
    • at E10.5-12.5, small number of embryos, presumably homozygous, have brain abnormalities, including a collapsed brain, pseudoencephaly or a narrowed brain region
    • from E14.5-17.5, some embryos show abnormal brain development (3/330) as described in younger embryos
    • myelencephalic blebs (MGI Ref ID J:3399)
      • one E17.5 embryo displayed a bleb near the midline in the cervical region
  • spina bifida (MGI Ref ID J:3399)
    • 4/330 embryos aged E14.5-17.5 displayed spina bifida
  • hematopoietic system phenotype
  • anemia (MGI Ref ID J:3399)
    • starting around E13.5 and peaking at E14.5, presumed homozygotes display anemia recognized by overall paleness of the embryos
  • cardiovascular system phenotype
  • abnormal blood circulation (MGI Ref ID J:3399)
    • in affected (anemic) animals, individual clumps or red blood cells can be seen in umbilical vessels; in controls, vessels are uniformly red with normal blood flow
  • skin/coat/nails phenotype
  • pallor (MGI Ref ID J:3399)
    • characteristic of anemic embryos
  • reproductive system phenotype
  • infertility (MGI Ref ID J:5547)
    • mice carrying two mutant alleles at the Kitl locus are sterile

Gene & Allele Details

Allele Symbol KitlSl
Allele Name steel
Common Name(s) MgfSl; Sl;
Strain of OriginC3H
Gene Symbol and Name Kitl, kit ligand
Chromosome 10
Gene Common Name(s) Clo; Con; DKFZp686F2250; Gb; KL-1; Kl1; Kl2; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
General Note Numerous experiments have demonstrated that precursors of pigment cells and blood cells of homozygotes behave normally when transplanted to an environment of wild-type cells, and that the locus exerts its effect through action of cells in the microenvironment of the apparently affected cells. This steel allele is semidominant. The original steel mutation arose spontaneously in the C3H inbred strain the Oak Ridge National Laboratory. Primordial germ cells are absent in KitlSl/KitlSl and deficient in KitlSl/+. There is no pigment-forming ability in the skin of KitlSl/KitlSl embryos (J:28098). KitlSl derived-fibroblasts are incapable of binding the KIT receptor, as normal fibroblasts can (J:10749). KitlSl is a deletion of the locus (J:10748)(J:6031).
Molecular Note By Southern blotting, it was concluded that this allele contains a deletion encompassing most, if not all, of the coding region of the gene. A probe corresponding to nucleotides 6 to 685 of the cDNA failed to hybridize to DNA obtained from embryos homozygous for this allele. PCR analysis with primers for sequences at various distances from the Kit gene narrowed the 5' and 3' deletion endpoints to a 350 and a 380 base-pair region, respectively. Sequencing of the product of PCR using primers designed to span the deletion revealed that it extends through 973,366 base pairs on Chromosome 10 between nucleotide positions 99,177,807 and 100,151,173 (NCBI Map Viewer, Build 36.1), with a 4-base pair insertion joining the deletion endpoints, and contains 6 predicted and 3 known genes. [MGI Ref ID J:10750] [MGI Ref ID J:115283]
 
Allele Symbol Pde6brd1
Allele Name retinal degeneration 1
Common Name(s) rd; rd-1; rd1; rodless retina;

Control Information

  Allele   Control
 KitlSl  Wild-type from the colony
 
  Considerations for Choosing Controls

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   KitlSl allele
000124   B6.Cg-KitlSl Krt71Ca/J
000291   C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying   KitlSl     (3 strains)

Strains carrying   Pde6brd1 allele
004202   B6.C3 Pde6brd1 Hps4le/+ +-Lmx1adr-8J/J
000002   B6.C3-Pde6brd1 Hps4le/J
001022   B6C3FeF1/J a/a
000652   BDP/J
000653   BUB/BnJ
002439   C3.129P2(B6)-B2mtm1Unc/J
005494   C3.129S1(B6)-Grm1rcw/J
000480   C3.MRL-Faslpr/J
001957   C3A Pde6brd1.O20/A-Prph2Rd2/J
005973   C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326   C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968   C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
001906   C3Ga.Cg-Catb/J
001904   C3H-Atcayji-hes/J
000659   C3H/HeJ
000784   C3H/HeJ-Faslgld/J
000509   C3H/HeJ-Lystbg-2J/J
002433   C3H/HeJ-Spnb4qv-lnd2J/J
005972   C3H/HeJBirLtJ
001824   C3H/HeJSxJ
000635   C3H/HeOuJ
000474   C3H/HeSn
001431   C3H/HeSn-ocd/J
000661   C3H/HeSnJ
002235   C3H/HeSnJ-Ctnna2cdf/J
002333   C3H/HeSnJ-gri/J
006435   C3HeB.SW-Soaa/MonJ
000658   C3HeB/FeJ
001576   C3HeB/FeJ-Atp7btx-J/J
002588   C3HeB/FeJ-Eya1bor/J
001533   C3HeB/FeJ-Mc1rE-so Gli3Xt-J/J
001886   C3HeB/FeJLe a/a-gnd/J
001908   C3HfB/BiJ
001502   C3Sn.B6-Epha4rb/J
001547   C3Sn.Cg-Cm/J
000656   CBA/J
000813   CBA/J-Atp7aMo-pew/J
000660   DA/HuSnJ
000023   FL/1ReJ
000025   FL/4ReJ
003024   FVB.129P2(B6)-Fmr1tm1Cgr/J
002539   FVB.129P2-Abcb4tm1Bor/J
002935   FVB.129S2(B6)-Ccnd1tm1Wbg/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
003078   FVB.Cg-Tg(WapIgf1)39Dlr/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
002374   FVB/N-Tg(MMTV-PyVT)634Mul/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
002384   FVB/N-Tg(UcpDta)1Kz/J
001800   FVB/NJ
003487   FVB/NJ-Tg(XGFAP-lacZ)3Mes/J
001491   FVB/NMob
000734   MOLD/RkJ
000550   MOLF/EiJ
002423   NON/ShiLtJ
000679   P/J
000680   PL/J
100299   PLSJLF1/J
000269   SB/LeJ
005651   SJL.AK-Thy1a/TseJ
000686   SJL/J
000688   ST/bJ
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002648   STOCK a/a Cln6nclf/J
000279   STOCK gr +/+ Ap3d1mh/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
004770   SW.B6-Soab/J
002023   SWR.M-Emv21 Emv22/J
000689   SWR/J
000939   SWR/J-Clcn1adr-mto/J
000692   WB/ReJ KitW/J
100410   WBB6F1/J-KitW/KitW-v/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying   Pde6brd1     (74 strains)

Strains carrying other alleles of Kitl
000090   129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
002993   B6.Cg-KitlSl-18H/EiJ
000160   B6.D2-KitlSl-d/J
001380   C3Sn.Cg-KitlSl-con/J
003252   C57BL/6J-KitlSl-20J/J
006839   C57BL/6J-KitlSl-22J/J
000979   STOCK KitlSl-16J/J
000161   WB.D2-KitlSl-d/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying other alleles of Kitl     (9 strains)

View Strains carrying other alleles of Pde6b     (8 strains)

Additional Web Information

Genetic Quality Control Annual Report
JAX Notes, April 1988; 433. H-2 Haplotypes of Mice from Jackson Laboratory Production Colonies.
JAX Notes, February 2001; 481. Mgf Gene Name Changes to Kitl.
JAX Notes, Spring 2002; 485. Genetic Background Effects: Can Your Mice See?

Animal Health Reports

Room Number           FGB27

Research Applications

This mouse can be used to support research in many areas including:

KitlSl related

Cancer Research
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence (Gonadal Tumors: ovarian and testicular)

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (Mast Cell Deficiency)

Neurobiology Research
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Fertility Defects
Gonadal Tumors (ovarian and testicular)

Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)

Sensorineural Research
Vestibular and Hearing Defects

Pde6brd1 related

Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive

Sensorineural Research
Retinal Degeneration

References

Selected Reference(s)

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Hayashi C; Sonoda T; Nakano T; Nakayama H; Kitamura Y. 1985. Mast-cell precursors in the skin of mouse embryos and their deficiency in embryos of Sl/Sld genotype. Dev Biol 109(1):234-41. [PubMed: 3987963]  [MGI Ref ID J:7810]

Huang E; Nocka K; Beier DR; Chu TY; Buck J; Lahm HW; Wellner D; Leder P; Besmer P. 1990. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63(1):225-33. [PubMed: 1698557]  [MGI Ref ID J:10751]

Murphy ED. 1977. Effects of mutant steel alleles on leukemogenesis and life-span in the mouse. J Natl Cancer Inst 58(1):107-10. [PubMed: 319242]  [MGI Ref ID J:5758]

Shinohara T; Avarbock MR; Brinster RL. 2000. Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models. Dev Biol 220(2):401-11. [PubMed: 10753526]  [MGI Ref ID J:61712]

Zsebo KM; Williams DA; Geissler EN; Broudy VC; Martin FH; Atkins HL; Hsu RY; Birkett NC; Okino KH; Murdock DC; Jacobsen FW; Langley KE; Smith KA; Takeishi T; Cattanach BM; Galli SJ; Suggs SV. 1990. Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63(1):213-24. [PubMed: 1698556]  [MGI Ref ID J:10750]

Additional References

Price and Supply Information

Strain Name: WC/ReJ KitlSl/J
Stock Number: 000693

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Mouse Mutant Resource collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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