Description

Strain Information

Former Names C57BL/6J-jsd/J    (Changed: 15-DEC-04 ) Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N1

Control Information

	Control
	Untyped from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Utp14b^jsd/Utp14b^jsd

        involves: C3H/HeJ * C57BL/6J

• endocrine/exocrine gland phenotype
• decreased testis weight (MGI Ref ID J:91782)
  • testes weight is significantly reduced at 6 weeks of age
• reproductive system phenotype
• azoospermia (MGI Ref ID J:91782)
  • no sperm are found in the testes
• decreased testis weight (MGI Ref ID J:91782)
  • testes weight is significantly reduced at 6 weeks of age
• male infertility (MGI Ref ID J:91782)
  • males are infertile

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Utp14b^jsd related

Endocrine Deficiency Research
Gonad Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Utp14bjsd
Allele Name		juvenile spermatogonial depletion
Allele Type		Spontaneous
Common Name(s)		jsd;
Strain of Origin		C57BL/6J
Gene Symbol and Name		Utp14b, UTP14, U3 small nucleolar ribonucleoprotein, homolog B (yeast)
Chromosome		1
Gene Common Name(s)		4932411L21Rik; RIKEN cDNA 4932411L21 gene; jsd; juvenile spermatogonial depletion;
General Note		Homozygous females are normal and fertile. Homozygous males have small testes, normal serum testosterone, elevated serum follicle-stimulating hormone, azoospermia, and normal body and seminal vesicle weights. At 3 to 4 weeks, there is a reduction in number of spermatogenic cells that proceeds at different rates in different tubules, but by 8 to 10 weeks most tubules are devoid of germ cells, leaving only Sertoli cells(J:9278).
Molecular Note		A CC to GAAAAG substitution 304 bp from the ATG putatively results in a frame shift such that after the first 105 amino acids, there are two substitutions (GE to LF) followed by a stop codon.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Beamer WG; Cunliffe-Beamer TL; Shultz KL; Langley SH; Roderick TH. 1988. Juvenile spermatogonial depletion (jsd): a genetic defect of germ cell proliferation of male mice. Biol Reprod 38(4):899-908. [PubMed: 3401545]  [MGI Ref ID J:9278]

Additional References

Boettger-Tong HL; Rohozinski J; Agoulnik AI; Dohmae K; Nishimune Y; Levy N; Bishop CE. 2001. Identification and sequencing the juvenile spermatogonial depletion critical interval on mouse chromosome 1 reveals the presence of eight candidate genes. Biochem Biophys Res Commun 288(5):1129-35. [PubMed: 11700028]  [MGI Ref ID J:72903]

Tanaka K; Tamura H; Tanaka H; Katoh M; Futamata Y; Seki N; Nishimune Y; Hara T. 2002. Spermatogonia-dependent expression of testicular genes in mice. Dev Biol 246(2):466-79. [PubMed: 12051830]  [MGI Ref ID J:77230]

Tohda A; Matsumiya K; Tadokoro Y; Yomogida K; Miyagawa Y; Dohmae K; Okuyama A; Nishimune Y. 2001. Testosterone suppresses spermatogenesis in juvenile spermatogonial depletion (jsd ) mice. Biol Reprod 65(2):532-7. [PubMed: 11466222]  [MGI Ref ID J:70679]

Tohda A; Okuno T; Matsumiya K; Okabe M; Kishikawa H; Dohmae K; Okuyama A; Nishimune Y. 2002. Restoration of spermatogenesis and fertility in azoospermic mutant mice by suppression and reelevation of testosterone followed by intracytoplasmic sperm injection. Biol Reprod 66(1):85-90. [PubMed: 11751268]  [MGI Ref ID J:74015]

Utp14b^jsd related

Beamer WG; Beamer TC; Shultz K. 1984. Juvenile spermatogonal depletion (jsd) Mouse News Lett 70:84.  [MGI Ref ID J:35669]

Bolden-Tiller OU; Chiarini-Garcia H; Poirier C; Alves-Freitas D; Weng CC; Shetty G; Meistrich ML. 2007. Genetic factors contributing to defective spermatogonial differentiation in juvenile spermatogonial depletion (Utp14bjsd) mice. Biol Reprod 77(2):237-46. [PubMed: 17475932]  [MGI Ref ID J:124196]

Bradley J; Baltus A; Skaletsky H; Royce-Tolland M; Dewar K; Page DC. 2004. An X-to-autosome retrogene is required for spermatogenesis in mice. Nat Genet 36(8):872-6. [PubMed: 15258580]  [MGI Ref ID J:91708]

Cooke HJ; Saunders PT. 2002. Mouse models of male infertility. Nat Rev Genet 3(10):790-801. [PubMed: 12360237]  [MGI Ref ID J:79958]

Kojima Y; Kominami K; Dohmae K; Nonomura N; Miki T; Okuyama A; Nishimune Y; Okabe M. 1997. Cessation of spermatogenesis in juvenile spermatogonial depletion (jsd/jsd) mice. Int J Urol 4(5):500-7. [PubMed: 9354954]  [MGI Ref ID J:44998]

Matsumiya K; Meistrich ML; Shetty G; Dohmae K; Tohda A; Okuyama A; Nishimune Y. 1999. Stimulation of spermatogonial differentiation in juvenile spermatogonial depletion (jsd) mutant mice by gonadotropin-releasing hormone antagonist treatment. Endocrinology 140(10):4912-5. [PubMed: 10499552]  [MGI Ref ID J:114216]

Mizunuma M; Dohmae K; Tajima Y; Koshimizu U; Watanabe D; Nishimune Y. 1992. Loss of sperm in juvenile spermatogonial depletion (jsd) mutant mice is ascribed to a defect of intratubular environment to support germ cell differentiation. J Cell Physiol 150(1):188-93. [PubMed: 1346141]  [MGI Ref ID J:1815]

Rohozinski J; Bishop CE. 2004. The mouse juvenile spermatogonial depletion (jsd) phenotype is due to a mutation in the X-derived retrogene, mUtp14b. Proc Natl Acad Sci U S A 101(32):11695-700. [PubMed: 15289605]  [MGI Ref ID J:91782]

Shetty G; Shao SH; Weng CC. 2008. p53-dependent apoptosis in the inhibition of spermatogonial differentiation in juvenile spermatogonial depletion (Utp14bjsd) mice. Endocrinology 149(6):2773-81. [PubMed: 18356279]  [MGI Ref ID J:136072]

Shetty G; Weng CC. 2004. Cryptorchidism rescues spermatogonial differentiation in juvenile spermatogonial depletion (jsd) mice. Endocrinology 145(1):126-33. [PubMed: 14500567]  [MGI Ref ID J:96500]

Shetty G; Weng CC; Porter KL; Zhang Z; Pakarinen P; Kumar TR; Meistrich ML. 2006. Spermatogonial differentiation in juvenile spermatogonial depletion (jsd) mice with androgen receptor or follicle-stimulating hormone mutations. Endocrinology 147(7):3563-70. [PubMed: 16627582]  [MGI Ref ID J:110487]

Shetty G; Wilson G; Huhtaniemi I; Boettger-Tong H; Meistrich ML. 2001. Testosterone inhibits spermatogonial differentiation in juvenile spermatogonial depletion mice. Endocrinology 142(7):2789-95. [PubMed: 11415997]  [MGI Ref ID J:71935]

Tohda A; Matsumiya K; Tadokoro Y; Yomogida K; Miyagawa Y; Dohmae K; Okuyama A; Nishimune Y. 2001. Testosterone suppresses spermatogenesis in juvenile spermatogonial depletion (jsd ) mice. Biol Reprod 65(2):532-7. [PubMed: 11466222]  [MGI Ref ID J:70679]

Tohda A; Okuno T; Matsumiya K; Okabe M; Kishikawa H; Dohmae K; Okuyama A; Nishimune Y. 2002. Restoration of spermatogenesis and fertility in azoospermic mutant mice by suppression and reelevation of testosterone followed by intracytoplasmic sperm injection. Biol Reprod 66(1):85-90. [PubMed: 11751268]  [MGI Ref ID J:74015]

Yomogida K; Ohtani H; Harigae H; Ito E; Nishimune Y; Engel JD; Yamamoto M. 1994. Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells. Development 120(7):1759-66. [PubMed: 7924983]  [MGI Ref ID J:19188]

de Rooij DG; Okabe M; Nishimune Y. 1999. Arrest of spermatogonial differentiation in jsd/jsd, Sl17H/Sl17H, and cryptorchid mice. Biol Reprod 61(3):842-7. [PubMed: 10456866]  [MGI Ref ID J:57059]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Untyped from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.