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Strain Name:

129P3/J-Leprdb-3J/J

Stock Number:

000709

Availability:

Repository-Cryopreserved

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General Terms and Conditions

Genes & Alleles   Lepr;   Leprdb-3J;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Spontaneous Mutation
Specieslaboratory mouse
GenerationN7F13N1

Strain Description
Mice homozygous for the diabetes 3J spontaneous mutation (Leprdb-3J) become identifiably obese around 3 to 4 weeks of age. Elevations of plasma insulin begin at 10 to 14 days and of blood sugar at 4 to 8 weeks. Homozygous mutant mice are polyphagic, polydipsic, and polyuric. The course of the disease is markedly influenced by genetic background. Homozygous mutant mice on the 129P3/J background exhibit severe obesity but the diabetes phenotype is much reduced.

Mammalian Phenotype Terms assigned by genotype

Leprdb-3J/Leprdb-3J

        129P3/J-Leprdb-3J/J
  • growth/size phenotype
  • increased body weight (MGI Ref ID J:6337)
    • obese (MGI Ref ID J:6337)
      • homozygotes accumulated excessive fat by 3 weeks of age
      • by 6 months of age, mice weighed from 70-90 grams compared to 20-28 grams in controls
  • weight loss (MGI Ref ID J:6337)
    • older mice exhibit sudden weight loss before death
  • behavior/neurological phenotype
  • polyphagia (MGI Ref ID J:6337)
  • endocrine/exocrine gland phenotype
  • abnormal exocrine pancreas morphology (MGI Ref ID J:6337)
    • necrosis of the exocrine, but not the endocrine pancreas was noted in aging animals
  • abnormal pancreatic alpha cell morphology (MGI Ref ID J:6337)
    • less than 2-fold increase in the number of alpha cells per islet
  • increased pancreatic beta cell number (MGI Ref ID J:6337)
    • beta cell hyperplasia
  • islet cell hyperplasia (MGI Ref ID J:6337)
  • homeostasis/metabolism phenotype
  • hyperglycemia (MGI Ref ID J:6337)
    • mutant males, but not females, were transiently hyperglycemic between 2 and 4 months of age
    • glucose levels declined thereafter
  • hypoglycemia (MGI Ref ID J:6337)
    • aged mice become hypoglycemic by 9 months of age
  • increased circulating glucagon level (MGI Ref ID J:6337)
    • plasma glucagon levels were increased by 3-fold in young mice and 5-fold in aged mice
    • pancreatic glucagon content was only doubled compared to controls at 8 months of age
  • increased circulating insulin level (MGI Ref ID J:6337)
    • at 5 months of age, plasma insulin levels were 30-fold greater in males, and 18-fold greater in females than in controls
    • pancreatic insulin levels were 30-fold greater than controls at 8 months of age
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:6337)
    • reduced lifespan, with 67% mortality between 6 and 14 months of age
  • reproductive system phenotype
  • infertility (MGI Ref ID J:6337)
    • mice are sterile
  • digestive/alimentary phenotype
  • abnormal exocrine pancreas morphology (MGI Ref ID J:6337)
    • necrosis of the exocrine, but not the endocrine pancreas was noted in aging animals
  • abnormal pancreatic alpha cell morphology (MGI Ref ID J:6337)
    • less than 2-fold increase in the number of alpha cells per islet
  • increased pancreatic beta cell number (MGI Ref ID J:6337)
    • beta cell hyperplasia
  • islet cell hyperplasia (MGI Ref ID J:6337)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Leprdb-3J/Leprdb-3J

        B6.129-Leprdb-3J
  • homeostasis/metabolism phenotype
  • abnormal glucose homeostasis (MGI Ref ID J:78850)
    • fasting mice at 3 months of age are euglycemic compaired to hyperglycemic obese mice on a congenic FVB background at 3 months
    • mice clear glucose load by 90 minutes, while on the FVB background, obese mice have severely impaired glucose tolerance
    • abnormal circulating glucose level (MGI Ref ID J:78850)
      • after a 2-day fast on refeeding with carbohydrate-free diet, female obese mice do not show a rise in glucose, while on the FVB background glucose levels increase
    • abnormal circulating insulin level (MGI Ref ID J:78850)
      • at 3 months, female obese mice have slightly lower insulin levels than FVB.BKS-Leprdb females; levels increase to ~50 ng/ml at 5-7 months compared to ~500 ng/ml for obese FVB.BKS-Leprdb females
    • increased insulin sensitivity (MGI Ref ID J:78850)
      • in response to 3U/kg insulin, blood glucose drops 50% in 40 minutes while no change is seen in mice on the FVB background

Gene & Allele Details

Allele Symbol Leprdb-3J
Allele Name diabetes 3 Jackson
Strain of Origin129P3/J
Gene Symbol and Name Lepr, leptin receptor
Chromosome 4
Gene Common Name(s) CD295; Fa; LEPROT; Leprb; Modb1; OB-RGRP; OBR; db; diabetes; leptin receptor gene-related protein; obese-like; obl;
Molecular Note Sequence analysis of the 3J allele revealed a 17 nucleotide deletion beginning at base 1874. This deletion results in a shift of the reading frame, and a premature termination of the protein at the 11th amino acid after the deletion site. The mutation is predicted to result in a truncated receptor without a transmembrane domain and affects all Lepr splice variants. [MGI Ref ID J:40658]

Control Information

  Control
   Untyped from the colony
   000690 129P3/J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Lepr     (10 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Research Applications

This mouse can be used to support research in many areas including:

Leprdb-3J related

Diabetes and Obesity Research
Hyperglycemia
Hyperinsulinemia
Insulin Resistance
Obesity With Diabetes
Type 2 Diabetes (NIDDM)

Endocrine Deficiency Research
Adipose Defects
Hypothalamus/Pituitary Defects
Pancreas Defects

Immunology and Inflammation Research
Immunodeficiency Associated with Other Defects

Internal/Organ Research
Adipose Defects

Metabolism Research

Mouse/Human Gene Homologs
obesity, morbid, with hypogonadism (rare)

Reproductive Biology Research
Fertility Defects

References

Additional References

Price and Supply Information

Strain Name: 129P3/J-Leprdb-3J/J
Stock Number: 000709

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery of Strains Needing Progeny Testing.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent.

Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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