Strain Name:

129P3/J-Leprdb-3J/J

Stock Number:

000709

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN7F13N1
Generation Definitions

Description
Mice homozygous for the diabetes 3J spontaneous mutation (Leprdb-3J) become identifiably obese around three to four weeks of age. Elevations of plasma insulin begin at 10 to 14 days and of blood sugar at four to eight weeks. Homozygous mutant mice are polyphagic, polydipsic, and polyuric. The course of the disease is markedly influenced by genetic background. Homozygous mutant mice on the 129P3/J background exhibit severe obesity but the diabetes phenotype is much reduced.

Control Information

  Control
   Untyped from the colony
   000690 129P3/J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Lepr     (18 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Obesity
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Leptin Receptor Deficiency   (LEPR)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Leprdb-3J/Leprdb-3J

        129P3/J-Leprdb-3J/J
  • mortality/aging
  • premature death
    • reduced lifespan, with 67% mortality between 6 and 14 months of age   (MGI Ref ID J:6337)
  • growth/size/body phenotype
  • increased body weight   (MGI Ref ID J:6337)
    • obese
      • homozygotes accumulated excessive fat by 3 weeks of age   (MGI Ref ID J:6337)
      • by 6 months of age, mice weighed from 70-90 grams compared to 20-28 grams in controls   (MGI Ref ID J:6337)
  • weight loss
    • older mice exhibit sudden weight loss before death   (MGI Ref ID J:6337)
  • behavior/neurological phenotype
  • polyphagia   (MGI Ref ID J:6337)
  • endocrine/exocrine gland phenotype
  • abnormal exocrine pancreas morphology
    • necrosis of the exocrine, but not the endocrine pancreas was noted in aging animals   (MGI Ref ID J:6337)
  • increased pancreatic alpha cell number
    • less than 2-fold increase in the number of alpha cells per islet   (MGI Ref ID J:6337)
  • increased pancreatic beta cell number
    • beta cell hyperplasia   (MGI Ref ID J:6337)
  • pancreatic islet hyperplasia   (MGI Ref ID J:6337)
  • homeostasis/metabolism phenotype
  • hyperglycemia
    • mutant males, but not females, were transiently hyperglycemic between 2 and 4 months of age   (MGI Ref ID J:6337)
    • glucose levels declined thereafter   (MGI Ref ID J:6337)
  • hypoglycemia
    • aged mice become hypoglycemic by 9 months of age   (MGI Ref ID J:6337)
  • increased circulating glucagon level
    • plasma glucagon levels were increased by 3-fold in young mice and 5-fold in aged mice   (MGI Ref ID J:6337)
    • pancreatic glucagon content was only doubled compared to controls at 8 months of age   (MGI Ref ID J:6337)
  • increased circulating insulin level
    • at 5 months of age, plasma insulin levels were 30-fold greater in males, and 18-fold greater in females than in controls   (MGI Ref ID J:6337)
    • pancreatic insulin levels were 30-fold greater than controls at 8 months of age   (MGI Ref ID J:6337)
  • reproductive system phenotype
  • infertility
    • mice are sterile   (MGI Ref ID J:6337)
  • digestive/alimentary phenotype
  • abnormal exocrine pancreas morphology
    • necrosis of the exocrine, but not the endocrine pancreas was noted in aging animals   (MGI Ref ID J:6337)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Leprdb-3J/Leprdb-3J

        B6.129-Leprdb-3J
  • homeostasis/metabolism phenotype
  • abnormal glucose homeostasis
    • fasting mice at 3 months of age are euglycemic compaired to hyperglycemic obese mice on a congenic FVB background at 3 months   (MGI Ref ID J:78850)
    • mice clear glucose load by 90 minutes, while on the FVB background, obese mice have severely impaired glucose tolerance   (MGI Ref ID J:78850)
    • abnormal circulating glucose level
      • after a 2-day fast on refeeding with carbohydrate-free diet, female obese mice do not show a rise in glucose, while on the FVB background glucose levels increase   (MGI Ref ID J:78850)
    • abnormal circulating insulin level
      • at 3 months, female obese mice have slightly lower insulin levels than FVB.BKS-Leprdb females; levels increase to ~50 ng/ml at 5-7 months compared to ~500 ng/ml for obese FVB.BKS-Leprdb females   (MGI Ref ID J:78850)
    • increased insulin sensitivity
      • in response to 3U/kg insulin, blood glucose drops 50% in 40 minutes while no change is seen in mice on the FVB background   (MGI Ref ID J:78850)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Leprdb-3J related

Diabetes and Obesity Research
Hyperglycemia
Hyperinsulinemia
Insulin Resistance
Obesity With Diabetes
Type 2 Diabetes (NIDDM)

Endocrine Deficiency Research
Adipose Defects
Hypothalamus/Pituitary Defects
Pancreas Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency Associated with Other Defects

Internal/Organ Research
Adipose Defects

Metabolism Research

Reproductive Biology Research
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Leprdb-3J
Allele Name diabetes 3 Jackson
Allele Type Spontaneous
Strain of Origin129P3/J
Gene Symbol and Name Lepr, leptin receptor
Chromosome 4
Gene Common Name(s) CD295; LEP-R; LEPRD; LEPROT; Leprb; Modb1; OB-R; OB-RGRP; OBR; db; diabetes; leptin receptor gene-related protein; obese-like; obl;
Molecular Note Sequence analysis of the 3J allele revealed a 17 nucleotide deletion beginning at base 1874. This deletion results in a shift of the reading frame, and a premature termination of the protein at the 11th amino acid after the deletion site. The mutation is predicted to result in a truncated receptor without a transmembrane domain and affects all Lepr splice variants. [MGI Ref ID J:40658]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Bahary N; Leibel RL; Joseph L; Friedman JM. 1990. Molecular mapping of the mouse db mutation. Proc Natl Acad Sci U S A 87(21):8642-6. [PubMed: 1978328]  [MGI Ref ID J:10819]

Barinaga M. 1996. Researchers nail down leptin receptor [news; comment] Science 271(5251):913. [PubMed: 8584929]  [MGI Ref ID J:31488]

Chen H; Charlat O; Tartaglia LA; Woolf EA; Weng X; Ellis SJ; Lakey ND; Culpepper J; Moore KJ; Breitbart RE; Duyk GM; Tepper RI; Morgenstern JP. 1996. Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell 84(3):491-5. [PubMed: 8608603]  [MGI Ref ID J:31324]

Chua SC Jr; Chung WK; Wu-Peng XS; Zhang Y; Liu SM; Tartaglia L; Leibel RL. 1996. Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor [see comments] Science 271(5251):994-6. [PubMed: 8584938]  [MGI Ref ID J:31419]

Coleman DL. 1978. Obese and diabetes: two mutant genes causing diabetes-obesity syndromes in mice. Diabetologia 14(3):141-8. [PubMed: 350680]  [MGI Ref ID J:5986]

Coleman DL; Hummel KP. 1973. The influence of genetic background on the expression of the obese (Ob) gene in the mouse. Diabetologia 9(4):287-93. [PubMed: 4588246]  [MGI Ref ID J:5400]

Hummel KP; Dickie MM; Coleman DL. 1966. Diabetes, a new mutation in the mouse. Science 153(740):1127-8. [PubMed: 5918576]  [MGI Ref ID J:5010]

Leiter EH; Chapman HD. 1994. Obesity-induced diabetes (diabesity) in C57BL/KsJ mice produces aberrant trans-regulation of sex steroid sulfotransferase genes. J Clin Invest 93(5):2007-13. [PubMed: 8182132]  [MGI Ref ID J:17991]

Leiter EH; Chapman HD; Coleman DL. 1989. The influence of genetic background on the expression of mutations at the diabetes locus in the mouse. V. Interaction between the db gene and hepatic sex steroid sulfotransferases correlates with gender-dependent susceptibility to hyperglycemia. Endocrinology 124(2):912-22. [PubMed: 2912706]  [MGI Ref ID J:26013]

Leiter EH; Coleman DL; Eisenstein AB; Strack I. 1980. A new mutation (db3J) at the diabetes locus in strain 129/J mice. I. Physiological and histological characterization. Diabetologia 19(1):58-65. [PubMed: 6993269]  [MGI Ref ID J:6337]

Trayhurn P. 1979. Thermoregulation in the diabetic-obese (db/db) mouse. The role of non-shivering thermogenesis in energy balance. Pflugers Arch 380(3):227-32. [PubMed: 573463]  [MGI Ref ID J:6202]

Leprdb-3J related

Chua S Jr; Liu SM; Li Q; Yang L; Thassanapaff VT; Fisher P. 2002. Differential beta cell responses to hyperglycaemia and insulin resistance in two novel congenic strains of diabetes (FVB- Lepr (db)) and obese (DBA- Lep (ob)) mice. Diabetologia 45(7):976-90. [PubMed: 12136396]  [MGI Ref ID J:78850]

Chua SC Jr; Chung WK; Wu-Peng XS; Zhang Y; Liu SM; Tartaglia L; Leibel RL. 1996. Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor [see comments] Science 271(5251):994-6. [PubMed: 8584938]  [MGI Ref ID J:31419]

Lee G; Li C; Montez J; Halaas J; Darvishzadeh J; Friedman JM. 1997. Leptin receptor mutations in 129 db3J/db3J mice and NIH facp/facp rats. Mamm Genome 8(6):445-7. [PubMed: 9166593]  [MGI Ref ID J:40658]

Leiter EH; Coleman DL; Eisenstein AB; Strack I. 1980. A new mutation (db3J) at the diabetes locus in strain 129/J mice. I. Physiological and histological characterization. Diabetologia 19(1):58-65. [PubMed: 6993269]  [MGI Ref ID J:6337]

Li C; Ioffe E; Fidahusein N; Connolly E; Friedman JM. 1998. Absence of soluble leptin receptor in plasma from dbPas/dbPas and other db/db mice. J Biol Chem 273(16):10078-82. [PubMed: 9545355]  [MGI Ref ID J:47037]

Luo N; Liu SM; Liu H; Li Q; Xu Q; Sun X; Davis B; Li J; Chua S Jr. 2006. Allelic variation on chromosome 5 controls beta-cell mass expansion during hyperglycemia in leptin receptor-deficient diabetes mice. Endocrinology 147(5):2287-95. [PubMed: 16484328]  [MGI Ref ID J:129660]

Yoon JW; Leiter EH; Coleman DL; Kim MK; Pak CY; McArthur RG; Roncari DA. 1988. Genetic control of organ-reactive autoantibody production in mice by obesity (ob) diabetes (db) genes. Diabetes 37(9):1287-93. [PubMed: 3044893]  [MGI Ref ID J:109940]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is no guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain, tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is no guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain, tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Untyped from the colony
   000690 129P3/J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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