Strain Name: |
B6;D2-a Es1e/J |
|---|---|
Stock Number: |
000785 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
STOCK a Es1e/J (Changed: 28-MAR-08
) |
| Genes & Alleles | Es1; a; ; |
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Species laboratory mouse Generation ?+F12N1p Strain Description
Mice homozygous for Es1e are viable and fertile and exhibit no apparent defect.Es1e was discovered in a screen of progeny of triethylenemelamine (TEM) treated male mice for mutations at specific loci, but appears to have pre-existed in the male. The screen employed analysis of blood and kidney homogenates by "standard starch gel electrophoresis techniques" and focused on enzymes known to differ in electrophoretic mobility between the parental strains (DBA/2J and C57BL/6J). Es1e was initially thought to be a null allele, but characterization of homozygous F2 mice demonstrated presence of a faint band migrating between those of the parental strains, which was not perceived in the presence of either parental band. Thus, Es1e was shown to be a hypomorphic electrophoretic variant (Soares 1979).
Strain Development
Es1e was discovered in a specific-locus mutation screen of progeny of triethylenemelamine (TEM) mutagenized male DBA/2J mice, but appears to have been a pre-existing, spontaneous mutation. Although the TEM-treated parent was unavailable for genotyping, three lines of evidence suggest he was a heterozygous carrier of the mutation: 7 of 13 (54%) of his immediate offspring were heterozygous for Es1e; he was mated within 2 weeks after TEM treatment, so these progeny were generated from sperm that were either sperm or late spermatids when exposed, ruling out a clustering effect due to mutagenesis at the spermatogonial stage; and the seven Es1e/+ offspring occurred among litters of three dams (Soares 1979).Mice of a strain/stock bearing Es1e, called ES-IE, were imported from Dr. Soares, then at NIEH, by Dr. Eva Eicher in 1979. The original stock was maintained by sister-brother inbreeding. In 1982, Es1e/Es1e males of this stock were bred to C57BL/6J females to produce embryos for cryopreservation. These heterozygous embryos were assigned Stock No. 000785.
| Allele Symbol | a | ||
|---|---|---|---|
| Allele Name | nonagouti | ||
| Gene Symbol and Name | Es1, esterase 1 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | Ee-1; Es-1; Es-4; Es-N; Es2; MGC109055; esterase 4; esterase related gene-N; | ||
Strains carrying a allele
View Strains carrying a (100 strains)
Metabolism Research
Selected Reference(s)
Soares ER. 1979. Identification of a new allele of Es-I segregating in an inbred strain of mice. Biochem Genet 17(7-8):577-83. [PubMed: 540008] [J:6287]
| Strain Name: | B6;D2-a Es1e/J |
| Stock Number: | 000785 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form