Description

Strain Information

Former Names C3H/HeJ-md2J    (Changed: 15-DEC-04 ) Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N1F2N1

Development
Mahoganoid 2 Jackson (Mgrn^md-2J) arose spontaneously in strain C3H/HeJ at F157 at The Jackson Laboratory. The mutation was maintained by sibling matings with md-2J segregating. At F157+F15 it was backcrossed once to C3H/HeJ. It was cryopreserved in 1984 by mating homozygous males at generation F157+F15N1F2 to C3H/HeJ females.

Related Strains

Strains carrying other alleles of Mgrn1

000001	B6.C3 A/a Mgrn1md/J
000309	B6.C3-Mgrn1md/J
000223	C3H/HeJ-Mgrn1md/J
001277	STOCK Mgrn1md Igl-1b Igl1-rlo/J

View Strains carrying other alleles of Mgrn1     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Mgrn1^md-2J/Mgrn1^md-2J

        C3H/HeJ-Mgrn1^md-2J

• pigmentation phenotype
• abnormal coat color (MGI Ref ID J:119125)
  • have dark backs but some agouti hairs on their flanks
• skin/coat/nails phenotype
• abnormal coat color (MGI Ref ID J:119125)
  • have dark backs but some agouti hairs on their flanks

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mgrn1^md-2J/Mgrn1^md-2J

        involves: 129S1/SvImJ * C3H/HeJ

• lethality-prenatal/perinatal
• perinatal lethality (MGI Ref ID J:116645)
  • 60% of homozygote die before birth or during the first 3 weeks of life
• prenatal lethality (MGI Ref ID J:116645)
  • 60% of homozygote die before birth or during the first 3 weeks of life
• lethality-postnatal
• postnatal lethality (MGI Ref ID J:116645)
  • 60% of homozygote die before birth or during the first 3 weeks of life
• growth/size phenotype
• left pulmonary isomerism (MGI Ref ID J:116645)
  • in some 12.5-18.5 d.p.c. homozygous embryos
• situs inversus (MGI Ref ID J:116645)
  • found in a very small proportion (<1%) of adult homozygous mice
• cardiovascular system phenotype
• abnormal artery morphology (MGI Ref ID J:116645)
  • malposition of the great artery of the heart in 12.5-18.5 d.p.c. homozygous embryo
• abnormal coronary artery morphology (MGI Ref ID J:116645)
  • unroofed coronary sinus in 12.5-18.5 d.p.c. homozygous embryos
• abnormal subclavian artery morphology (MGI Ref ID J:116645)
  • retroesophageal left subclavian artery in 12.5-18.5 d.p.c. homozygous embryo
• right aortic arch (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• abnormal atrial septum morphology (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• abnormal ventricular septum morphology (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• congestive heart failure (MGI Ref ID J:116645)
  • in some 12.5-18.5 d.p.c. homozygous embryos caused by congenital heart defects
• dextrocardia (MGI Ref ID J:116645)
  • in some 12.5-18.5 d.p.c. homozygous embryos
• double outlet right ventricle (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• hemorrhage (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• mesocardia (MGI Ref ID J:116645)
  • n some 12.5-18.5 d.p.c. homozygous embryos
• pericardial effusion (MGI Ref ID J:116645)
  • pericardial and pleural effusion in some 12.5-18.5 d.p.c. homozygous embryos
• thin myocardial wall (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• muscle phenotype
• thin myocardial wall (MGI Ref ID J:116645)
  • in 12.5-18.5 d.p.c. homozygous embryos
• respiratory system phenotype
• abnormal lung morphology (MGI Ref ID J:116645)
  • reversed lung situs in some 12.5-18.5 d.p.c. homozygous embryos
• left pulmonary isomerism (MGI Ref ID J:116645)
  • in some 12.5-18.5 d.p.c. homozygous embryos
• embryogenesis phenotype
• abnormal left-right axis patterning (MGI Ref ID J:116645)
  • embryonic lethality resulted from congenital heart defects due to defective establishment and/or maintenance of the left-right axis

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Mgrn1^md-2J related

Dermatology Research
Color and White Spotting Defects
Skin and Hair Texture Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mgrn1md-2J
Allele Name		mahoganoid 2 Jackson
Allele Type		Spontaneous
Common Name(s)		md2J;
Strain of Origin		C3H/HeJ
Gene Symbol and Name		Mgrn1, mahogunin, ring finger 1
Chromosome		16
Gene Common Name(s)		2610042J20Rik; KIAA0544; N28182; RGD1311862; RIKEN cDNA 2610042J20 gene; RNF156; expressed sequence N28182; mKIAA0544; mahoganoid; md; nc; non-agouti curly; non-agouti-curly;
General Note		Genbank ID for this allele: AF532997, AF532998
Molecular Note		The mutation in md2J has been attributed to an 8 kb retroviral insertion within exon 12. Northern analysis and RT-PCR indicate that the expression of transcript is greatly reduced in these mice. [MGI Ref ID J:80182]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Mgrn1^md-2J related

Bagher P; Jiao J; Owen Smith C; Cota CD; Gunn TM. 2006. Characterization of Mahogunin Ring Finger-1 expression in mice. Pigment Cell Res 19(6):635-43. [PubMed: 17083490]  [MGI Ref ID J:119125]

Cota CD; Bagher P; Pelc P; Smith CO; Bodner CR; Gunn TM. 2006. Mice with mutations in Mahogunin ring finger-1 (Mgrn1) exhibit abnormal patterning of the left-right axis. Dev Dyn 235(12):3438-47. [PubMed: 17075880]  [MGI Ref ID J:116645]

Phan LK; Lin F; LeDuc CA; Chung WK; Leibel RL. 2002. The mouse mahoganoid coat color mutation disrupts a novel C3HC4 RING domain protein. J Clin Invest 110(10):1449-59. [PubMed: 12438443]  [MGI Ref ID J:80182]

Sweet HO; Davisson MT. 1995. Remutations at The Jackson Laboratory (Update to Mouse Genome 1993; 91:862-5 - J16313) Mouse Genome 93(4):1030-4.  [MGI Ref ID J:30778]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.