Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names AKXD-28/TyJ    (Changed: 15-DEC-04 ) Type Recombinant Inbred (RI); Additional information on Recombinant Inbred Mice. Visit our online Nomenclature tutorial. Species laboratory mouse RI progenitor AKR/J DBA/2J H2 Haplotype k Generation F51pF26

Appearance
chocolate brown (nonagouti brown)
Related Genotype: Typr1^b/Tyrp1^b Myo5a⁺/Myo5a⁺

Description
Similar to DBA/2J, AKXD28 mice develop an age-related glaucoma characterized by intraocular pressure elevation, retinal ganglion loss, and optic nerve excavation. Although both glaucoma related alleles, Gpnmb^R150X and Tyrp1^isa, are present in AKXD28, the phenotype differs from DBA/2J. AKXD28 mice do not develop iris pigment dispersion (IPD) and develop a more severe retinal damage.

Related Strains

AKXD Strains

001005	AKXD1/TyJ
001017	AKXD10/TyJ
001003	AKXD11/TyJ
000765	AKXD13/TyJ
000779	AKXD14/TyJ
000954	AKXD15/TyJ
000958	AKXD16/TyJ
001093	AKXD18/TyJ
000776	AKXD2/TyJ
001001	AKXD20/TyJ
001062	AKXD21/TyJ
000947	AKXD22/TyJ
000780	AKXD23/TyJ
000969	AKXD24/TyJ
000949	AKXD25/TyJ
000764	AKXD27/TyJ
000959	AKXD3/TyJ
000777	AKXD6/TyJ
000763	AKXD9/TyJ

View AKXD Strains     (19 strains)

Strains carrying   Gpnmb^R150X allele

000671

DBA/2J

View Strains carrying   Gpnmb^R150X     (1 strain)

Strains carrying   Tyrp1^isa allele

000671

DBA/2J

View Strains carrying   Tyrp1^isa     (1 strain)

Strains carrying other alleles of Gpnmb

007048

DBA/2J-Gpnmb+/SjJ

View Strains carrying other alleles of Gpnmb     (1 strain)

Strains carrying other alleles of Tyrp1

000004	ABP/LeJ
000093	B6.B10(D1)-Tyrp1b-c/J
008684	B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J
000571	B6.Cg-Whrnwi Tyrp1b/+ +/J
000027	B6.D-Tyrp1b Dock7m/J
000068	C57BL/6J-Tyrp1b-J/J
000670	DBA/1J
006252	LT/SvEiJ
000265	MY/HuLeJ
001045	SI/Col Tyrp1b Dnahc11iv/J
002142	STOCK 11R30m/J
000064	STOCK a Tyrp1b Sisi/J
002238	STOCK a Tyrp1b shmy/J
001432	STOCK a/a Tyrp1b sks/Tyrp1b +/J
000594	STOCK T(2;8)26H a/T(2;8)26H a Tyrp1+/Tyrp1b/J
001101	STOCK T(3;4)5Rk Tyrp1b/J
000274	TSJ/LeJ

View Strains carrying other alleles of Tyrp1     (17 strains)

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Mouse Phenome Database
Wellcome Trust Centre for Human Genetics: Mouse Recombinant Inbred Line (RIL) Genotype Data for AKXD RI Line

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

       

• tumorigenesis
• increased resistance to lymphoma (MGI Ref ID J:4179)
  • unlike most other AKXD RI strains of this set, the majority of 20 females survived to 18 months of age in good health and lymphomas were not the major cause of death
• lymphoblastic lymphoma (MGI Ref ID J:69716)
  • of 3 lymphomas characterized 1 was a T cell lymphoblastic lymphoma
• splenic marginal zone lymphoma (MGI Ref ID J:69716)
  • of 3 lymphomas characterized 2 were splenic marginal zone lymphomas

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
      genes regulating lymphoma development
Genetics Research
      Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis

Sensorineural Research
Eye Defects
      hereditary glaucoma

Genes & Alleles

Gene & Allele Information

Allele Symbol		GpnmbR150X
Allele Name		iris pigment dispersion
Allele Type		Spontaneous
Common Name(s)		Gpnmbipd;
Strain of Origin		DBA/2J
Gene Symbol and Name		Gpnmb, glycoprotein (transmembrane) nmb
Chromosome		6
Gene Common Name(s)		Dchil; HGFIN; NMB; Osteoactivin; dendritic cell associated heparan sulfate proteoglycans dependent integrin ligand; ipd; iris pigment dispersion;
General Note		The mouse strain DBA/2J develops glaucoma associated with iris stromal atrophy and iris pigment dispersion phneotypes. Genetic studies defined two separate loci that contribute to the overall phenotype in the DBA/2J mouse, ipd and isa. Either mutation in a homozygous state contributes to glaucoma, while mice homozygous for both mutations develop an earlier onset and more severe iris disease.
Molecular Note		The underlying mutation responsible for the phenotype in the iris pigment dispersion mouse was identified as a C to T substitution that resulted in a nonsense mutation, Arg150stop. [MGI Ref ID J:75398]
Allele Symbol		Tyrp1isa
Allele Name		iris stromal atrophy
Allele Type		Spontaneous
Common Name(s)		isa;
Strain of Origin		DBA/2J
Gene Symbol and Name		Tyrp1, tyrosinase-related protein 1
Chromosome		4
Gene Common Name(s)		B; CAS2; CATB; GP75; TRP; TRP-1; TRP1; TYRP; Tyrp; b; b-PROTEIN; brown; iris stromal atrophy; isa; tyrosinase-related protein;
General Note		The mouse strain DBA/2J develops glaucoma associated with iris stromal atrophy and iris pigment dispersion phneotypes. Genetic studies defined two separate loci that contribute to the overall phenotype in the DBA/2J mouse, ipd and isa. Either mutation in a homozygous state contributes to glaucoma, while mice homozygous for both mutations develop an earlier onset and more severe iris disease.
Molecular Note		Expression of a BAC containing the wild-type Tyrp1 gene rescues the phenotype of isa mice. The isa phenotype has been identified in numerous aged stocks carrying Tyrp1b allele. It is therefore most probable, that the Tyrp1b alleleis responsible for the isa phenotype. [MGI Ref ID J:75398]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Anderson MG; Smith RS; Savinova OV; Hawes NL; Chang B; Zabaleta A; Wilpan R; Heckenlively JR; Davisson M; John SW. 2001. Genetic modification of glaucoma associated phenotypes between AKXD-28/Ty and DBA/2J mice. BMC Genet 2(1):1. [PubMed: 11178107]  [MGI Ref ID J:82878]

Gpnmb^R150X related

Abdelmagid SM; Barbe MF; Rico MC; Salihoglu S; Arango-Hisijara I; Selim AH; Anderson MG; Owen TA; Popoff SN; Safadi FF. 2008. Osteoactivin, an anabolic factor that regulates osteoblast differentiation and function. Exp Cell Res 314(13):2334-51. [PubMed: 18555216]  [MGI Ref ID J:138006]

Anderson MG; Libby RT; Mao M; Cosma IM; Wilson LA; Smith RS; John SW. 2006. Genetic context determines susceptibility to intraocular pressure elevation in a mouse pigmentary glaucoma. BMC Biol 4:20. [PubMed: 16827931]  [MGI Ref ID J:128215]

Anderson MG; Nair KS; Amonoo LA; Mehalow A; Trantow CM; Masli S; John SW. 2008. GpnmbR150X allele must be present in bone marrow derived cells to mediate DBA/2J glaucoma. BMC Genet 9:30. [PubMed: 18402690]  [MGI Ref ID J:134670]

Anderson MG; Smith RS; Hawes NL; Zabaleta A; Chang B; Wiggs JL; John SW. 2002. Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice. Nat Genet 30(1):81-5. [PubMed: 11743578]  [MGI Ref ID J:75398]

Anderson MG; Smith RS; Savinova OV; Hawes NL; Chang B; Zabaleta A; Wilpan R; Heckenlively JR; Davisson M; John SW. 2001. Genetic modification of glaucoma associated phenotypes between AKXD-28/Ty and DBA/2J mice. BMC Genet 2(1):1. [PubMed: 11178107]  [MGI Ref ID J:82878]

Chang B; Smith RS; Hawes NL; Anderson MG; Zabaleta A; Savinova O ; Roderick TH ; Heckenlively JR ; Davisson MT ; John SW. 1999. Interacting loci cause severe iris atrophy and glaucoma in DBA/2J mice. Nat Genet 21(4):405-9. [PubMed: 10192392]  [MGI Ref ID J:54013]

Howell GR; Libby RT; Jakobs TC; Smith RS; Phalan FC; Barter JW; Barbay JM; Marchant JK; Mahesh N; Porciatti V; Whitmore AV; Masland RH; John SW. 2007. Axons of retinal ganglion cells are insulted in the optic nerve early in DBA/2J glaucoma. J Cell Biol 179(7):1523-37. [PubMed: 18158332]  [MGI Ref ID J:131073]

Inman DM; Sappington RM; Horner PJ; Calkins DJ. 2006. Quantitative correlation of optic nerve pathology with ocular pressure and corneal thickness in the DBA/2 mouse model of glaucoma. Invest Ophthalmol Vis Sci 47(3):986-96. [PubMed: 16505033]  [MGI Ref ID J:108329]

Jakobs TC; Libby RT; Ben Y; John SW; Masland RH. 2005. Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice. J Cell Biol 171(2):313-25. [PubMed: 16247030]  [MGI Ref ID J:104518]

Libby RT; Li Y; Savinova OV; Barter J; Smith RS; Nickells RW; John SW. 2005. Susceptibility to neurodegeneration in a glaucoma is modified by bax gene dosage. PLoS Genet 1(1):e4. [PubMed: 16103918]  [MGI Ref ID J:100118]

Ripoll VM; Irvine KM; Ravasi T; Sweet MJ; Hume DA. 2007. Gpnmb is induced in macrophages by IFN-gamma and lipopolysaccharide and acts as a feedback regulator of proinflammatory responses. J Immunol 178(10):6557-66. [PubMed: 17475886]  [MGI Ref ID J:146106]

Zhou X; Li F; Kong L; Tomita H; Li C; Cao W. 2005. Involvement of inflammation, degradation, and apoptosis in a mouse model of glaucoma. J Biol Chem 280(35):31240-8. [PubMed: 15985430]  [MGI Ref ID J:101163]

Tyrp1^isa related

Anderson MG; Smith RS; Hawes NL; Zabaleta A; Chang B; Wiggs JL; John SW. 2002. Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice. Nat Genet 30(1):81-5. [PubMed: 11743578]  [MGI Ref ID J:75398]

Anderson MG; Smith RS; Savinova OV; Hawes NL; Chang B; Zabaleta A; Wilpan R; Heckenlively JR; Davisson M; John SW. 2001. Genetic modification of glaucoma associated phenotypes between AKXD-28/Ty and DBA/2J mice. BMC Genet 2(1):1. [PubMed: 11178107]  [MGI Ref ID J:82878]

Chang B; Smith RS; Hawes NL; Anderson MG; Zabaleta A; Savinova O ; Roderick TH ; Heckenlively JR ; Davisson MT ; John SW. 1999. Interacting loci cause severe iris atrophy and glaucoma in DBA/2J mice. Nat Genet 21(4):405-9. [PubMed: 10192392]  [MGI Ref ID J:54013]

Inman DM; Sappington RM; Horner PJ; Calkins DJ. 2006. Quantitative correlation of optic nerve pathology with ocular pressure and corneal thickness in the DBA/2 mouse model of glaucoma. Invest Ophthalmol Vis Sci 47(3):986-96. [PubMed: 16505033]  [MGI Ref ID J:108329]

Jakobs TC; Libby RT; Ben Y; John SW; Masland RH. 2005. Retinal ganglion cell degeneration is topological but not cell type specific in DBA/2J mice. J Cell Biol 171(2):313-25. [PubMed: 16247030]  [MGI Ref ID J:104518]

John SW; Anderson MG; Smith RS. 1999. Mouse genetics: a tool to help unlock the mechanisms of glaucoma. J Glaucoma 8(6):400-12. [PubMed: 10604301]  [MGI Ref ID J:59796]

Libby RT; Li Y; Savinova OV; Barter J; Smith RS; Nickells RW; John SW. 2005. Susceptibility to neurodegeneration in a glaucoma is modified by bax gene dosage. PLoS Genet 1(1):e4. [PubMed: 16103918]  [MGI Ref ID J:100118]

Zhou X; Li F; Kong L; Tomita H; Li C; Cao W. 2005. Involvement of inflammation, degradation, and apoptosis in a mouse model of glaucoma. J Biol Chem 280(35):31240-8. [PubMed: 15985430]  [MGI Ref ID J:101163]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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