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Former Names B6Ei.Cg-TOrl/EiJ (Changed: 09-APR-07 ) B6Ei.Cg-TOrl (Changed: 05-APR-07 ) Type Congenic; Mutant Strain; Species laboratory mouse Background Strain C57BL/6JEiJ Donor Strain Non inbred Swiss/Orl stock Generation N16p Development
The TOrl mutation arose spontaneously in approximately 1972 in the Swiss/Orl non-inbred stock. It was backcrossed several generations onto C57BL/6JOrl and imported into the laboratory of Dr. Eva Eicher at The Jackson Laboratory where it was backcrossed onto the C57BL/6J background. In 1987 C57BL/6J females were bred with heterozygous males at generation N15 to generate embryos for cryopreservation.
Strains carrying other alleles of T
003879 B10;TFLe-a/a T tf/+ tf/J 000405 B10ScSn.Cg-T/J 004591 B6 x B6Ei.Cg-TWis/EiJ 000953 B6 x BALB/cBy-T4J/J 001518 B6 x STOCK T tf/th45 tf/J 000567 B6.Cg-T2J +/+ Qkqk/J 001015 B6.Cg-T4Or/J 000350 B6By.Cg-KitW-v MitfMi-wh T/J 002282 BTBR T+ tf/J 001053 C3Sn.AK-Thp/EiJ 000545 C57BL/6J-T2J/J 001199 C57BL/6J-T5J/J 001961 C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei 001814 STOCK Tc/J View Strains carrying other alleles of T (14 strains)
Congenic Nomenclature
Mammalian Phenotype Terms assigned by genotype
TOrl/T+
B6.Cg-TOrl/EiJ
- reproductive system phenotype
- primary sex reversal (MGI Ref ID J:71077)
- on a B6 background, mice carrying this allele and a Y chromosome from AKR, LEWES/Ei, MA/MyJ, PL/J, RF/J, or the WSB/Ei inbred strain develop as infertile females chromosomal males carrying this allele with a Y chromosome from an AKR, LEWES/Ei, MA/MyJ, PL/J, RF/J, or WSB/Ei background develop as fertile females
- chromosomal males carrying this allele with a Y chromosome from a BUB/BnJ, C57BL/6JEi, SJL/J, ST/bJ, or SWR/J develop as males on a B6 background, mice carrying this allele and a Y chromosome derived from BUB/BnJ, C57BL/6JEi, SJL/J, ST/bJ, or the SWR/J inbred strain develop as males
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
TOrl/T+
involves: C57BL/6J * Orl:Swiss
| Allele Symbol | TOrl | ||
|---|---|---|---|
| Allele Name | T-Orleans | ||
| Common Name(s) | TtOrl; | ||
| Strain of Origin | Orl:Swiss | ||
| Gene Symbol and Name | T, brachyury | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | Bra; Low; Lr; MGC104817; T1; TFT; Tl2; Tl3; brachyury-like 2; brachyury-like 3; cou; coupe; low ratio; me75; | ||
| General Note | TOrl, T-Orleans, semidominant. Arose spontaneously in a noninbred Swiss/Orl stock (J:15078). TOrl/+ Qk mice are short-tailed and quaking, suggesting that TOrl is a deletion involving both the T and Qk loci. TOrl is indistinguishable from Thp except that it does not have a maternal effect (J:11933). However, it is inseparably associated with a chromosomal region derived from a naturally occurring t haplotype. Its t-like properties are a consequence of closely linked t-haplotype genes, not of the deletion itself (J:6957). Silver et al. (J:6957) suggest the symbol Tt-Orl for this mutant complex. | ||
| Molecular Note | The deletion comprising this allele includes T through, at least, Qk as all short-tailed mice from matings of TOrl/+ males to Qkqk/Qkqk females have a "quaking" phenotype. In addition this allele expresses the t- haplotype specific proteins p63/6.9a, TCP-4B, TCP-5, TCP-9, and TCP-8B. [MGI Ref ID J:6957] | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455] [MGI Ref ID J:71077]
TOrl related
Bennett D. 1975. The T-locus of the mouse. Cell 6:441-454. [MGI Ref ID J:11933]Moutier R. 1973. t Mouse News Lett 49:42. [MGI Ref ID J:15078]
Silver LM; Lukralle D; Garrels JI. 1983. TOr1 is a novel, variant form of mouse chromosome 17 with a deletion in a partial t haplotype. Nature 301(5899):422-4. [PubMed: 6823317] [MGI Ref ID J:6957]
Wang E; Cortopassi G. 1994. Mice with duplications and deletions at the Tme locus have altered MnSOD activity. J Biol Chem 269(36):22463-5. [PubMed: 8077189] [MGI Ref ID J:20170]
Colony Maintenance
Breeding & Husbandry A large percent (~25%) of heterozygotes develop severe spina bifida and can not be used as breeders.
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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