JAX Mice Database - 001054 B6.Cg-T<Orl>/EiJ

Strain Name:	B6.Cg-T^Orl/EiJ
Stock Number:	001054
Availability:	Repository-Cryopreserved

Description
Phenotype
Genes & alleles
Genotyping
Health & husbandry
References
Purchasing information

Description

Strain Information

Former Names B6Ei.Cg-T^Orl/EiJ (Changed: 09-APR-07 )

B6Ei.Cg-T^Orl (Changed: 05-APR-07 )

Type Congenic; Mutant Strain;

Species laboratory mouse

Background Strain C57BL/6JEiJ

Donor Strain Non inbred Swiss/Orl stock

Generation N16p

Development
The T^Orl mutation arose spontaneously in approximately 1972 in the Swiss/Orl non-inbred stock. It was backcrossed several generations onto C57BL/6JOrl and imported into the laboratory of Dr. Eva Eicher at The Jackson Laboratory where it was backcrossed onto the C57BL/6J background. In 1987 C57BL/6J females were bred with heterozygous males at generation N15 to generate embryos for cryopreservation.

Related Strains

Strains carrying other alleles of T

003879 B10;TFLe-a/a T tf/+ tf/J

000405 B10ScSn.Cg-T/J

004591 B6 x B6Ei.Cg-T^Wis/EiJ

000953 B6 x BALB/cBy-T^4J/J

001518 B6 x STOCK T tf/t^h45 tf/J

000567 B6.Cg-T^2J +/+ Qk^qk/J

001015 B6.Cg-T^4Or/J

000350 B6By.Cg-Kit^W-v Mitf^Mi-wh T/J

002282 BTBR T⁺ tf/J

001053 C3Sn.AK-T^hp/EiJ

000545 C57BL/6J-T^2J/J

001199 C57BL/6J-T^5J/J

001961 C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei

001814 STOCK T^c/J

View Strains carrying other alleles of T (14 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

T^Orl/T⁺
B6.Cg-T^Orl/EiJ
reproductive system phenotype

primary sex reversal (MGI Ref ID J:71077)

on a B6 background, mice carrying this allele and a Y chromosome from AKR, LEWES/Ei, MA/MyJ, PL/J, RF/J, or the WSB/Ei inbred strain develop as infertile females chromosomal males carrying this allele with a Y chromosome from an AKR, LEWES/Ei, MA/MyJ, PL/J, RF/J, or WSB/Ei background develop as fertile females

chromosomal males carrying this allele with a Y chromosome from a BUB/BnJ, C57BL/6JEi, SJL/J, ST/bJ, or SWR/J develop as males on a B6 background, mice carrying this allele and a Y chromosome derived from BUB/BnJ, C57BL/6JEi, SJL/J, ST/bJ, or the SWR/J inbred strain develop as males

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.
T^Orl/T⁺
involves: C57BL/6J * Orl:Swiss
limbs/digits/tail phenotype

abnormal tail morphology (MGI Ref ID J:15078)

short tail (MGI Ref ID J:15078)

Genes & Alleles

Gene & Allele Information

Allele Symbol		T^Orl
Allele Name		T-Orleans
Common Name(s)		Tt^Orl;
Strain of Origin		Orl:Swiss
Gene Symbol and Name		T, brachyury
Chromosome		17
Gene Common Name(s)		Bra; Low; Lr; MGC104817; T1; TFT; Tl2; Tl3; brachyury-like 2; brachyury-like 3; cou; coupe; low ratio; me75;
General Note		T^Orl, T-Orleans, semidominant. Arose spontaneously in a noninbred Swiss/Orl stock (J:15078). T^Orl/+ Qk mice are short-tailed and quaking, suggesting that T^Orl is a deletion involving both the T and Qk loci. T^Orl is indistinguishable from Thp except that it does not have a maternal effect (J:11933). However, it is inseparably associated with a chromosomal region derived from a naturally occurring t haplotype. Its t-like properties are a consequence of closely linked t-haplotype genes, not of the deletion itself (J:6957). Silver et al. (J:6957) suggest the symbol T^t-Orl for this mutant complex.
Molecular Note		The deletion comprising this allele includes T through, at least, Qk as all short-tailed mice from matings of T^Orl/+ males to Qk^qk/Qk^qk females have a "quaking" phenotype. In addition this allele expresses the t- haplotype specific proteins p63/6.9a, TCP-4B, TCP-5, TCP-9, and TCP-8B. [MGI Ref ID J:6957]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

Selected Reference(s)

Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455] [MGI Ref ID J:71077]

Additional References

T^Orl related
Bennett D. 1975. The T-locus of the mouse. Cell 6:441-454. [MGI Ref ID J:11933]
Moutier R. 1973. t Mouse News Lett 49:42. [MGI Ref ID J:15078]
Silver LM; Lukralle D; Garrels JI. 1983. TOr1 is a novel, variant form of mouse chromosome 17 with a deletion in a partial t haplotype. Nature 301(5899):422-4. [PubMed: 6823317] [MGI Ref ID J:6957]
Wang E; Cortopassi G. 1994. Mice with duplications and deletions at the Tme locus have altered MnSOD activity. J Biol Chem 269(36):22463-5. [PubMed: 8077189] [MGI Ref ID J:20170]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry A large percent (~25%) of heterozygotes develop severe spina bifida and can not be used as breeders.

Breeding & Husbandry	A large percent (~25%) of heterozygotes develop severe spina bifida and can not be used as breeders.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Weeks of Age Price* Gender

Cryorecovery Fee $1900.00

*Price(s) in US dollars ($)

Weeks of Age	Price*	Gender
Cryorecovery Fee	$1900.00

Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. *Cryorecovery to establish a Dedicated Supply for greater quantities of mice.* One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX^® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX^® Services, Tel: 1-800-422-6423 or 1-207-288-5845.
Genomic DNA is available for this strain from the Mouse DNA Resource.

Pricing for International shipping destinations View USA, Canada and Mexico Pricing

Weeks of Age Price* Gender

Cryorecovery Fee $2470.00

*Price(s) in US dollars ($)

Weeks of Age	Price*	Gender
Cryorecovery Fee	$2470.00

Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. *Cryorecovery to establish a Dedicated Supply for greater quantities of mice.* One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX^® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX^® Services, Tel: 1-800-422-6423 or 1-207-288-5845.
Genomic DNA is available for this strain from the Mouse DNA Resource.

View USA, Canada and Mexico Pricing View International Pricing

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Ordering and Purchasing Information

Purchasing Information
JAX^® Mice Orders
Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

003879	B10;TFLe-a/a T tf/+ tf/J
000405	B10ScSn.Cg-T/J
004591	B6 x B6Ei.Cg-T^Wis/EiJ
000953	B6 x BALB/cBy-T^4J/J
001518	B6 x STOCK T tf/t^h45 tf/J
000567	B6.Cg-T^2J +/+ Qk^qk/J
001015	B6.Cg-T^4Or/J
000350	B6By.Cg-Kit^W-v Mitf^Mi-wh T/J
002282	BTBR T⁺ tf/J
001053	C3Sn.AK-T^hp/EiJ
000545	C57BL/6J-T^2J/J
001199	C57BL/6J-T^5J/J
001961	C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei
001814	STOCK T^c/J

Strain Name:

B6.Cg-TOrl/EiJ

Stock Number:

001054

Availability:

Repository-Cryopreserved

Description

Strain Information

Related Strains

Additional Web Information

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

TOrl/T+

TOrl/T+

Genes & Alleles

Gene & Allele Information

Genotyping

Genotyping Information

Helpful Links

References

References

Selected Reference(s)

Additional References

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Supply Details

General Terms and Conditions

The Jackson Laboratory's Genotype Promise

B6.Cg-T^Orl/EiJ

T^Orl/T⁺

T^Orl/T⁺