Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6Ei.Cg-TOrl/EiJ    (Changed: 09-APR-07 ) B6Ei.Cg-TOrl    (Changed: 05-APR-07 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6JEiJ Donor Strain Non inbred Swiss/Orl stock Generation N16p

Development
The T^Orl mutation arose spontaneously in approximately 1972 in the Swiss/Orl non-inbred stock. It was backcrossed several generations onto C57BL/6JOrl and imported into the laboratory of Dr. Eva Eicher at The Jackson Laboratory where it was backcrossed onto the C57BL/6J background. In 1987 C57BL/6J females were bred with heterozygous males at generation N15 to generate embryos for cryopreservation.

Related Strains

Strains carrying other alleles of T

003879	B10;TFLe-a/a T tf/+ tf/J
000405	B10ScSn.Cg-T/J
004591	B6 x B6Ei.Cg-TWis/EiJ
000953	B6 x BALB/cBy-T4J/J
001518	B6 x STOCK T tf/th45 tf/J
000567	B6.Cg-T2J +/+ Qkqk/J
001015	B6.Cg-T4Or/J
000350	B6By.Cg-KitW-v MitfMi-wh T/J
002282	BTBR T+ tf/J
001053	C3Sn.AK-Thp/EiJ
000545	C57BL/6J-T2J/J
001199	C57BL/6J-T5J/J
001961	C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei
001814	STOCK Tc/J

View Strains carrying other alleles of T     (14 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

T^Orl/T⁺

        B6.Cg-T^Orl/EiJ

• reproductive system phenotype
• primary sex reversal (MGI Ref ID J:71077)
  • on a B6 background, mice carrying this allele and a Y chromosome from AKR, LEWES/Ei, MA/MyJ, PL/J, RF/J, or the WSB/Ei inbred strain develop as infertile females chromosomal males carrying this allele with a Y chromosome from an AKR, LEWES/Ei, MA/MyJ, PL/J, RF/J, or WSB/Ei background develop as fertile females
  • chromosomal males carrying this allele with a Y chromosome from a BUB/BnJ, C57BL/6JEi, SJL/J, ST/bJ, or SWR/J develop as males on a B6 background, mice carrying this allele and a Y chromosome derived from BUB/BnJ, C57BL/6JEi, SJL/J, ST/bJ, or the SWR/J inbred strain develop as males

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

T^Orl/T⁺

        involves: C57BL/6J * Orl:Swiss

• limbs/digits/tail phenotype
• abnormal tail morphology (MGI Ref ID J:15078)
• short tail (MGI Ref ID J:15078)

Genes & Alleles

Gene & Allele Information

Allele Symbol		TOrl
Allele Name		T-Orleans
Allele Type		Spontaneous
Common Name(s)		T(t-Orl;
Strain of Origin		Orl:Swiss
Gene Symbol and Name		T, brachyury
Chromosome		17
Gene Common Name(s)		Bra; Low; Lr; MGC104817; T1; TFT; Tl2; Tl3; brachyury-like 2; brachyury-like 3; cou; coupe; low ratio; me75;
Molecular Note		The deletion comprising this allele includes T through, at least, Qk as all short-tailed mice from matings of TOrl/+ males to Qkqk/Qkqk females have a "quaking" phenotype. In addition this allele expresses the t- haplotype specific proteins p63/6.9a, TCP-4B, TCP-5, TCP-9, and TCP-8B. [MGI Ref ID J:6957]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455]  [MGI Ref ID J:71077]

Additional References

T^Orl related

Bennett D. 1975. The T-locus of the mouse. Cell 6:441-454.  [MGI Ref ID J:11933]

Moutier R. 1973. t Mouse News Lett 49:42.  [MGI Ref ID J:15078]

Silver LM; Lukralle D; Garrels JI. 1983. TOr1 is a novel, variant form of mouse chromosome 17 with a deletion in a partial t haplotype. Nature 301(5899):422-4. [PubMed: 6823317]  [MGI Ref ID J:6957]

Wang E; Cortopassi G. 1994. Mice with duplications and deletions at the Tme locus have altered MnSOD activity. J Biol Chem 269(36):22463-5. [PubMed: 8077189]  [MGI Ref ID J:20170]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	A large percent (~25%) of heterozygotes develop severe spina bifida and can not be used as breeders.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). Genomic DNA is available for this strain from the Mouse DNA Resource.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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