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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129T1/Sv-Oca2+ Tyrc-ch-Aft/J (Changed: 31-MAY-11 ) 129T1/Sv-p+ Tyrc-ch-Aft/J (Changed: 11-FEB-08 ) Type Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N14F3p
Generation DefinitionsDescription
Aft is a dominant mutation that causes increased postnatal lethality in heterozygotes and nearly 100% embryonic or perinatal lethality in homozygotes. Aft/+ mice can be identified by tail kinks near the tail tip and syndactyly of the third and fourth digits of the hind feet, a trait which is often unilateral. This syndactyly does not result from bone fusion, but rather from the persistence of the skin web that normally is removed via apoptosis during development. The tail kinks appear to result from cartilage overgrowth and fusion. Alopecia is found at six to eight months of age and can progress into bleeding ulcerations. Histology of Aft/+ skin shows fewer follicles, fibrosis, and an increase in the number of mast cells. The penetrance of this mutation is only approximately 65% on the 129/Sv and C57BL/6J backgrounds and the expressivity is variable. The most prevalent trait in Aft/+ mice is tail kinks, followed by syndactyly then skin lesions. The Aft mutation interacts with T such that double heterozygosity for Aft and T together increases the likelihood of having no tail relative to heterozygosity for T alone. Aft maps within the vicinity of brachyury modifier 1 on chromosome 9. (Lane, 1987; Ruvinsky et al., 2002.)Development
Aft arose spontaneously in 1984 on the 129/Sv-p+ Tyrc-ch-KitW-54J background in the laboratory of Dr. Jane Barker at The Jackson Laboratory. KitW-54J was bred away from Aft and in 1988 N14F2 heterozygotes of either gender were bred with 129/Sv mates to generate embryos for cryopreservation.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Wild-type from the colony | ||
| 002065 129T2/SvEmsJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Oca2+ allele
000090 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J 000091 129T1/Sv-Oca2+ Tyrc-ch Dnd1Ter/J 000822 B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J 000494 J.Cg-Oca2+ Tyr+ Lystbg/J View Strains carrying Oca2+ (4 strains)
000090 129S1/Sv-Oca2+ Tyr+ KitlSl-J/J 000091 129T1/Sv-Oca2+ Tyrc-ch Dnd1Ter/J 000004 ABP/LeJ 000822 B6 x 129S1/SvEi Oca2+ Tyr+-Vsx2or-J/J 000577 B6 x STOCK a Oca2p Hps5ru2 Ednrbs/J 001059 B6By.Cg-Oca2p/J 002460 C3H/HeJ-Oca2p-J Is(7;1)40H/J 000513 C3H/HeJ-Oca2p-J/J 001136 C57BL/6J-Oca2p-un+2J/J 001506 C57BL/6J-Oca2p-un+3J/J 001810 C57BL/6J-Oca2p-un+4J/J 001513 C57BL/6J-Oca2p-un+5J/J 001499 C57BL/6J-Oca2p-un+6J/J 001033 C57BL/6J-Oca2p-un+J/J 000028 C57BL/6J-Oca2p-un/J 000619 FS/EiJ 000494 J.Cg-Oca2+ Tyr+ Lystbg/J 000306 STOCK Dll3pu + Tyrc-ch/+ Oca2p Tyrc-ch/J 001584 STOCK Oca2p-J/Oca2p-bs/J 001585 STOCK Oca2p-d/Oca2p-25H/J 000823 STOCK Oca2p-d/Oca2p-6H/J 001747 STOCK Oca2p-d/Oca2p-cp/J 001618 STOCK Oca2p Prop1df/J
Additional Web Information
New 129 Nomenclature Bulletin
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Aft/Aft+
involves: 129S1/Sv
- limbs/digits/tail phenotype
- kinked tail
- 50% penetrance (MGI Ref ID J:12972)
- syndactyly
- syndactyly of digits 3 and 4 of the hindfeet, 50% penetrance (MGI Ref ID J:12972)
Aft/Aft+
involves: 129S1/Sv * C57BL/6J
- limbs/digits/tail phenotype
- kinked tail
- tail kinks are most likely caused by varying degrees of cartilage overgrowth and fusion and a dysmorphogenesis of bony elements attached to the fusion site (MGI Ref ID J:81166)
- syndactyly
- interdigital webbing
- syndactyly is caused by the persistence of the skin web between the digits and not due to fused bony elements (MGI Ref ID J:81166)
- integument phenotype
- alopecia
- incompletely penetrant progressive late-onset hair loss (around 6-8 months of age) that starts locally around the snout and back of ears (MGI Ref ID J:81166)
- decreased hair follicle number
- skin shows focal areas of decreased hair follicle density with fibrous tracts, pigment incontinence and increased mast cells (MGI Ref ID J:81166)
- disheveled coat
- at around 6-8 months of age, when local hair loss begins, the fur appears clumpy and unkempt (MGI Ref ID J:81166)
- skin lesions
- late-onset (6-8 months of age) of skin lesions following hair loss that progress into bleeding ulcerations (MGI Ref ID J:81166)
This mouse can be used to support research in many areas including:Aft related
Developmental Biology Research
Skeletal Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Aft | ||
|---|---|---|---|
| Allele Name | abnormal feet and tail | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | 129S1/Sv-KitW-54J Tyrc-ch | ||
| Gene Symbol and Name | Aft, abnormal feet and tail | ||
| Chromosome | 9 | ||
| Allele Symbol | Oca2+ | ||
| Allele Name | wild type | ||
| Allele Type | Not Specified | ||
| Gene Symbol and Name | Oca2, oculocutaneous albinism II | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | BEY; BEY1; BEY2; BOCA; D15S12; D7H15S12; D7Icr28RN; D7Nic1; DNA segment, Chr 7, Institute for Cancer Research 28RN; DNA segment, Chr 7, Nicholls 1; DNA segment, Chr 7, human D15S12; EYCL; EYCL2; EYCL3; HCL3; P; PED; SHEP1; p; pink-eyed dilution; | ||
References
References provided by MGI
Additional References
Ruvinsky I; Chertkov O; Borue X th; Agulnik SI; Gibson-Brown JJ; Lyle SR; Silver LM. 2002. Genetics analysis of mouse mutations Abnormal feet and tail and rough coat, which cause developmental abnormalities and alopecia. Mamm Genome 13(12):675-9. [PubMed: 12514744] [MGI Ref ID J:81166]
Aft relatedOca2+ relatedLane PW. 1987. Abnormal feet and tail (Aft) Mouse News Lett 78:56-57. [MGI Ref ID J:12972]
Ruvinsky I; Chertkov O; Borue X th; Agulnik SI; Gibson-Brown JJ; Lyle SR; Silver LM. 2002. Genetics analysis of mouse mutations Abnormal feet and tail and rough coat, which cause developmental abnormalities and alopecia. Mamm Genome 13(12):675-9. [PubMed: 12514744] [MGI Ref ID J:81166]
The RIKEN BioResource Center. 2006. Information obtained from The RIKEN BioResource Center Unpublished :. [MGI Ref ID J:104881]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| Wild-type from the colony | ||
| 002065 129T2/SvEmsJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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