Strain Name:

B6.Cg-Igha Thy1a Gpi1a/J

Stock Number:

001317

Availability:

Repository- Live

Description

Strain Information

Former Names C57BL/6J-Igh-1a Thy1a Gpi1a    (Changed: 15-DEC-04 )
C57BL/6J-Igha Thy1a Gpi1a    (Changed: 15-DEC-04 )
C57BL/6J-Igha Thy1a Gpi1a/J    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain B6.C-Igha, B6.PL-Thy1a
GenerationF60 (28-JAN-09)

Appearance
black
Related Genotype: a/a

Description
This congenic strain was made by mating together, not backcrossing, three individual congenic strains carrying differential alleles already on a C57BL/6J genetic background (B6.C-Igha, B6.PL-Thy1a, and B6.CAST-Gpi1a). This triple congenic is useful as a cellular marker in transplantation studies in conjunction with C57BL/6J inbred mice (Ighb, Thy1b, Gpi1b). Igh is a B cell specific marker, Thy1 is a T cell specific surface marker, and Gpi1 is an enzyme that is widely expressed in mouse tissues, cultured fibroblasts, and in several types of tumors.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   Gpi1a     (8 strains)

View Strains carrying   Thy1a     (14 strains)

View Strains carrying other alleles of Gpi1     (10 strains)

View Strains carrying other alleles of Igh     (13 strains)

View Strains carrying other alleles of Thy1     (29 strains)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers: B cell specific marker
Immunology and Inflammation Research
      B cell specific surface marker

Gpi1a related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers

Thy1a related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
      T cell specific surface marker

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Gpi1a
Allele Name a variant
Allele Type Not Applicable
Gene Symbol and Name Gpi1, glucose phosphate isomerase 1
Chromosome 7
Gene Common Name(s) AMF; GNPI; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; MF; NK; NK/GPI; NLK; Org; PGI; PHI; SA36; autocrine motility factor; glucose phosphate isomerase 1, regulatory; glucose phosphate isomerase 1, structural; glucose phosphate isomerase 1, temporal; maturation factor; neuroleukin;
Molecular Note This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1b. Two differences were silent, while a third difference at position 282 predicts an aspartate for the a allele and an asparagine for the b allele. A fourth difference in the 3' untranslated region was also noted. [MGI Ref ID J:28033]
 
Allele Symbol Thy1a
Allele Name a variant
Allele Type Not Applicable
Common Name(s) Thy-1.1; Thy1.1; Thy1a; theta-AKR; thetaAKR;
Gene Symbol and Name Thy1, thymus cell antigen 1, theta
Chromosome 9
Gene Common Name(s) CD7; CD90; FLJ33325; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2; theta;
General Note

The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that isthe same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).

 
Gene Symbol and Name Igh, immunoglobulin heavy chain complex
Chromosome 12

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Fabien N; Bergerot I; Maguer-Satta V; Orgiazzi J; Thivolet C. 1995. Pancreatic lymph nodes are early targets of T cells during adoptive transfer of diabetes in NOD mice. J Autoimmun 8(3):323-34. [PubMed: 7575994]  [MGI Ref ID J:26213]

Trowbridge IS; Mazauskas C. 1976. Immunological properties of murine thymus-dependent lymphocyte surface glycoproteins. Eur J Immunol 6(8):557-62. [PubMed: 61878]  [MGI Ref ID J:5707]

Additional References

Gpi1a related

Charles DJ; Lee CY. 1980. Biochemical and immunological characterization of genetic variants of phosphoglucose isomerase from mouse. Biochem Genet 18(1-2):153-69. [PubMed: 6155905]  [MGI Ref ID J:6330]

Chen J; Reifsnyder PC; Scheuplein F; Schott WH; Mileikovsky M; Soodeen-Karamath S; Nagy A; Dosch MH; Ellis J; Koch-Nolte F; Leiter EH. 2005. 'Agouti NOD': identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells. Mamm Genome 16(10):775-83. [PubMed: 16261419]  [MGI Ref ID J:102639]

Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866]  [MGI Ref ID J:107466]

Collinson JM; Quinn JC; Hill RE; West JD. 2003. The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo. Dev Biol 255(2):303-12. [PubMed: 12648492]  [MGI Ref ID J:82632]

DeLorenzo RJ; Ruddle FH. 1969. Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem Genet 3(2):151-62. [PubMed: 5364923]  [MGI Ref ID J:5141]

Ito CY; Li CY; Bernstein A; Dick JE; Stanford WL. 2003. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice. Blood 101(2):517-23. [PubMed: 12393491]  [MGI Ref ID J:81387]

Pearce SR; Morgan MJ; Ball S; Peters J; Faik P. 1995. Sequence characterization of alleles Gpi1-Sa and Gpi1-Sb at the glucose phosphate isomerase structural locus. Mamm Genome 6(8):537-9. [PubMed: 8589524]  [MGI Ref ID J:28033]

Szuber N; Buss JL; Soe-Lin S; Felfly H; Trudel M; Ponka P. 2008. Alternative treatment paradigm for thalassemia using iron chelators. Exp Hematol 36(7):773-85. [PubMed: 18456387]  [MGI Ref ID J:136991]

Thy1a related

Beck PL; Li Y; Wong J; Chen CW; Keenan CM; Sharkey KA; McCafferty DM. 2007. Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation. Gastroenterology 132(5):1778-90. [PubMed: 17449036]  [MGI Ref ID J:128325]

Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866]  [MGI Ref ID J:107466]

D'Eustachio P; Owens GC; Edelman GM; Cunningham BA. 1985. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A 82(22):7631-5. [PubMed: 3865183]  [MGI Ref ID J:8111]

Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063]  [MGI Ref ID J:113109]

Green MC; Sweet HO. 1975. [Hx - Hm - W.] Mouse News Lett 52:38.  [MGI Ref ID J:13675]

Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin.  [MGI Ref ID J:110093]

REIF AE; ALLEN JM. 1964. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med 120:413-33. [PubMed: 14207060]  [MGI Ref ID J:24839]

Ranheim EA; Tarbell KV; Krogsgaard M; Mallet-Designe V; Teyton L; McDevitt HO; Weissman IL. 2004. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity 37(8):555-67. [PubMed: 15763918]  [MGI Ref ID J:128250]

Read S; Hogan TV; Zwar TD; Gleeson PA; Van Driel IR. 2007. Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells. Gastroenterology 133(2):547-58. [PubMed: 17603058]  [MGI Ref ID J:128303]

Reif AE; Allen JM. 1966. Mouse thymic iso-antigens. Nature 209(22):521-3. [PubMed: 5919593]  [MGI Ref ID J:5012]

Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198]  [MGI Ref ID J:133382]

Wuthrich M; Warner T; Klein BS. 2005. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol 175(8):5288-97. [PubMed: 16210634]  [MGI Ref ID J:119110]

Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566]  [MGI Ref ID J:126126]

Zaleski M; Klein J. 1974. Immune response of mice to Thy-1. 1 antigen: genetic control by alleles at the Ir-5 locus loosely linked to the H-2 complex. J Immunol 113(4):1170-7. [PubMed: 4606643]  [MGI Ref ID J:5487]

Zaleski MB. 1975. Immune response of mice to the Thy-1.1 antigen: effect of the Ir-5 alleles studies in 129/J and B10.129(6M) mice Immunogenetics 2:241-8.  [MGI Ref ID J:30773]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $95.30Female or MaleHomozygous for Igha, Homozygous for Thy1a, Homozygous for Gpi1a

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $123.90Female or MaleHomozygous for Igha, Homozygous for Thy1a, Homozygous for Gpi1a

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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