Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names MgfSl-con    (Changed: 15-DEC-04 ) Type Congenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C3H/HeSnJ Donor Strain (C3H/HeH x 101/H)F1 Generation N12p

Description
Both homozygous and heterozygous mice with the contrasted induced mutation (Kitl^Sl-con) are viable. Male homozygous mutant mice are fertile but females are usually sterile. Mice heterozygous for the contrasted mutation are recognizable soon after birth by dark pigmentation of the genital papilla with the adult coat being slightly lighter than normal. Homozygous mutant mice also have dark genitalia and a markedly diluted coat and mild macrocytic anemia.

Control Information

	Control
	Wild-type from the colony
	000661 C3H/HeSnJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Kitl

000090	129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
002993	B6.Cg-KitlSl-18H/EiJ
008656	B6.Cg-KitlSl-gb/MbeJ
000124	B6.Cg-KitlSl Krt71Ca/J
000160	B6.D2-KitlSl-d/J
000291	C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
003252	C57BL/6J-KitlSl-20J/J
000979	STOCK KitlSl-16J/J
000161	WB.D2-KitlSl-d/J
000693	WC/ReJ KitlSl/J
100401	WCB6F1/J KitlSl KitlSl-d

View Strains carrying other alleles of Kitl     (11 strains)

Additional Web Information

JAX^® NOTES, February 2001; 481. Mgf Gene Name Changes to Kitl.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Kitl^Sl-con/Kitl⁺

        either: (involves: C3H/HeJ) or (involves: C3H/HeN)

• pigmentation phenotype
• abnormal skin pigmentation (MGI Ref ID J:23593)
• hyperpigmentation (MGI Ref ID J:23593)
  • hyperpigmentation of the genital papillae
• reproductive system phenotype
• *normal* reproductive system phenotype (MGI Ref ID J:23593)
  • both males and females were fertile
• decreased oocyte cell number (MGI Ref ID J:23593)
• skin/coat/nails phenotype
• *normal* skin/coat/nails phenotype (MGI Ref ID J:23593)
  • normal coat color
• abnormal skin pigmentation (MGI Ref ID J:23593)

Kitl^Sl-con/Kitl^Sl-con

        either: (involves: C3H/HeJ) or (involves: C3H/HeN)

• pigmentation phenotype
• abnormal coat/hair pigmentation (MGI Ref ID J:23593)
  • uniformly gray coat
• abnormal skin pigmentation (MGI Ref ID J:23593)
• hyperpigmentation (MGI Ref ID J:23593)
  • hyperpigmentation of the genitals
  • occasional abnormal pigmentation of regions of the oviduct due to the accumulation of melanocytes
• endocrine/exocrine gland phenotype
• impaired ovarian folliculogenesis (MGI Ref ID J:23593)
  • follicular development is initially delayed, and although follicular development is relatively normal around 4 weeks of age, follicles fail to be maintained after 5 weeks of age
• hematopoietic system phenotype
• increased mast cell number (MGI Ref ID J:23593)
  • accumulation of mast cells in the reproductive tract, with the highest degree of accumulation in the serosal membrane
• immune system phenotype
• increased mast cell number (MGI Ref ID J:23593)
  • accumulation of mast cells in the reproductive tract, with the highest degree of accumulation in the serosal membrane
• reproductive system phenotype
• *normal* reproductive system phenotype (MGI Ref ID J:23593)
  • no defects were observed in the postnatal testes
• decreased oocyte cell number (MGI Ref ID J:23593)
• impaired ovarian folliculogenesis (MGI Ref ID J:23593)
  • follicular development is initially delayed, and although follicular development is relatively normal around 4 weeks of age, follicles fail to be maintained after 5 weeks of age
• skin/coat/nails phenotype
• abnormal coat/hair pigmentation (MGI Ref ID J:23593)
  • uniformly gray coat
• abnormal skin pigmentation (MGI Ref ID J:23593)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Kitl^Sl-con related

Cancer Research
Growth Factors/Receptors/Cytokines

Dermatology Research
Color and White Spotting Defects

Developmental Biology Research
Neural Crest Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Hypothalamus/Pituitary Defects
Skin Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
      Mast Cell Deficiency

Neurobiology Research
Vestibular and Hearing Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient
Fertility Defects
Gonadal Tumors
      testicular teratomas

Research Tools
Immunology and Inflammation Research
      Mast Cell Deficiency

Sensorineural Research
Vestibular and Hearing Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		KitlSl-con
Allele Name		contrasted
Allele Type		Radiation induced
Common Name(s)		Mgf;
Gene Symbol and Name		Kitl, kit ligand
Chromosome		10
Gene Common Name(s)		Clo; Con; DKFZp686F2250; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
General Note		The contrasted mutation was found in a neutron irradiation experiment. Heterozygotes are recognizable soon after birth by dark pigmentation of the genital papilla. The adult coat is slightly lighter than normal. Homozygotes also have dark external genitalia but a markedly diluted coat; they have a mild macrocytic anemia. Males are fertile but females are usually sterile (J:20215). The mutation was shown to be allelic to steel by linkage and complementation tests (J:13654). The cause of the Kitl mutation is a DNA rearrangement 5' from the Kitl coding region which disrupts initiation and maintenance of ovarian follicle development (J:23593.
Molecular Note		Pulse field gel electrophoresis using cDNA and genomic clones showed that this allele is an undefined rearrangement between 90 and 2.2Mb 5' to Kitl. Northern analysis showed that the mRNA transcript is of normal size but this allele exerts tissue-specific effects on transcript abundance. [MGI Ref ID J:23593]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Arguello F; Furlanetto RW; Baggs RB; Graves BT; Harwell SE; Cohen HJ; Frantz CN. 1992. Incidence and distribution of experimental metastases in mutant mice with defective organ microenvironments (genotypes Sl/Sld and W/Wv). Cancer Res 52(8):2304-9. [PubMed: 1559233]  [MGI Ref ID J:468]

Hayashi C; Sonoda T; Nakano T; Nakayama H; Kitamura Y. 1985. Mast-cell precursors in the skin of mouse embryos and their deficiency in embryos of Sl/Sld genotype. Dev Biol 109(1):234-41. [PubMed: 3987963]  [MGI Ref ID J:7810]

Huang E; Nocka K; Beier DR; Chu TY; Buck J; Lahm HW; Wellner D; Leder P; Besmer P. 1990. The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63(1):225-33. [PubMed: 1698557]  [MGI Ref ID J:10751]

Murphy ED. 1977. Effects of mutant steel alleles on leukemogenesis and life-span in the mouse. J Natl Cancer Inst 58(1):107-10. [PubMed: 319242]  [MGI Ref ID J:5758]

Schrott A; Egg G; Spoendlin H. 1988. Intermediate filaments in the cochleas of normal and mutant (w/wv, sl/sld) mice. Arch Otorhinolaryngol 245(4):250-4. [PubMed: 2460075]  [MGI Ref ID J:9423]

Schrott A; Spoendlin H. 1987. Pigment anomaly-associated inner ear deafness. Acta Otolaryngol (Stockh) 103(5-6):451-7. [PubMed: 3618172]  [MGI Ref ID J:8813]

Wolf NS. 1978. Dissecting the hematopoietic microenvironment. II. The kinetics of the erythron of the S1/S1d mouse and the dual nature of its anemia. Cell Tissue Kinet 11(4):325-34. [PubMed: 688326]  [MGI Ref ID J:6031]

Zsebo KM; Williams DA; Geissler EN; Broudy VC; Martin FH; Atkins HL; Hsu RY; Birkett NC; Okino KH; Murdock DC; Jacobsen FW; Langley KE; Smith KA; Takeishi T; Cattanach BM; Galli SJ; Suggs SV. 1990. Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63(1):213-24. [PubMed: 1698556]  [MGI Ref ID J:10750]

Kitl^Sl-con related

Bedell MA; Brannan CI; Evans EP; Copeland NG; Jenkins NA; Donovan PJ. 1995. DNA rearrangements located over 100 kb 5' of the Steel (Sl)-coding region in Steel-panda and Steel-contrasted mice deregulate Sl expression and cause female sterility by disrupting ovarian follicle development. Genes Dev 9(4):455-70. [PubMed: 7533739]  [MGI Ref ID J:23593]

Cattanach BM; Rasberry C. 1988. A new steel allele with early post-implantation homozygous lethality Mouse News Lett 80:157-58.  [MGI Ref ID J:18280]

Kelly EM. 1974. Cloud-gray is an allele of Sl Mouse News Lett 50:52.  [MGI Ref ID J:13654]

Peters J. 1987. A new steel allele which does not lead to dilution of coat colour Mouse News Lett 77:125-6.  [MGI Ref ID J:24739]

Searle AG. 1968. New mutant (Contrasted) Mouse News Lett 38:22.  [MGI Ref ID J:20215]

van Buul PP; Tuinenburg-Bol Raap A; Goudzwaard HJ; Seelen CM; Beechey CV; Natarajan AT; Searle AG. 1991. Cytogenetic characterization of radiosensitive mouse mutants. Mutat Res 251(2):171-9. [PubMed: 1720867]  [MGI Ref ID J:4646]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	000661 C3H/HeSnJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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phone:	207-288-6470
fax:	207-288-6655

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