Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK lop13/J    (Changed: 27-OCT-11 ) NUC    (Changed: 15-DEC-04 ) Type Mutant Stock; Additional information on Genetically Engineered and Mutant Mice. Type Inbred Strain; Additional information on Inbred Strains. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F35p Generation Definitions

Appearance
albino
Related Genotype: A^w/? Tyr^c/Tyr^c

Description
Lens opacity 13 is a recessive mutation that causes bilateral cataracts that do not affect the cortex of the lens. This presents at 3 to 4 weeks of age as a white area in the center of the lens. The size of the eyes is normal. (Varnum, 1981.)

Development
The lens opacity 13 mutation arose spontaneously on the 129/Sv-ter background and was first reported in 1981. It was crossed to 129X1/SvJ and may have had some A/HeJ bred in as well. Homozygous embryos from homozygous parents at F34 were frozen in 1990. (Varnum, 1981.)

Control Information

	Control
	None Available
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Srebf2^lop13/Srebf2^lop13

        STOCK Srebf2^lop13/J

• vision/eye phenotype
• *normal* vision/eye phenotype
  • despite lens opacity, mice exhibit normal lens cortex and eye size   (MGI Ref ID J:13874)
• • abnormal lens fiber morphology
    • abnormalities of the lens fiber cells, such as swollen cortical fibers and bladder cells, are seen   (MGI Ref ID J:176249)
    • degeneration of lens fibers is seen   (MGI Ref ID J:176249)
  • cataracts
    • mice can be identified at 3 to 4 weeks of age by a bilateral white area in the center of the lens   (MGI Ref ID J:13874)
    • between 4 and 10 weeks of age nuclear cataracts progress to mature cataracts   (MGI Ref ID J:176249)
    • by 3 months of age all mice display hypermature cataracts   (MGI Ref ID J:176249)
  • • nuclear cataracts
      • bilateral nuclear cataracts at P28   (MGI Ref ID J:176249)
• integument phenotype
• abnormal skin condition
  • develop persistent wounds of the skin in areas around the eye and on the back of the neck at 3 months of age   (MGI Ref ID J:176249)
  • once formed, wounds do not heal   (MGI Ref ID J:176249)
• homeostasis/metabolism phenotype
• decreased cholesterol level
  • decreased level in the lens   (MGI Ref ID J:176249)
  • however, no difference in serum cholesterol levels is detected   (MGI Ref ID J:176249)
• • decreased brain cholesterol level   (MGI Ref ID J:176249)
  • decreased liver cholesterol level   (MGI Ref ID J:176249)
• liver/biliary system phenotype
• decreased liver cholesterol level   (MGI Ref ID J:176249)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Srebf2^lop13 related

Developmental Biology Research
Eye Defects

Sensorineural Research
Cataracts
      nuclear
Eye Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Srebf2lop13
Allele Name		lens opacity 13
Allele Type		Spontaneous
Strain of Origin		129T1/Sv-Dnd1
Gene Symbol and Name		Srebf2, sterol regulatory element binding factor 2
Chromosome		15
Gene Common Name(s)		AI608257; SREBP-2; SREBP2; SREBP2gc; Srebf2_retired; bHLHd2; expressed sequence AI608257; lens opacity 13; lop13; nuc; nuclear cataract;
Molecular Note		A C to T mutation at position 3112 in exon 18 resulting in an arginine to cysteine substitution at amino acid 1038. [MGI Ref ID J:176249]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Merath KM; Chang B; Dubielzig R; Jeannotte R; Sidjanin DJ. 2011. A spontaneous mutation in Srebf2 leads to cataracts and persistent skin wounds in the lens opacity 13 (lop13) mouse. Mamm Genome :. [PubMed: 21858719]  [MGI Ref ID J:176249]

Varnum D. 1981. Nuclear cataract (nuc) Mouse News Lett 64:59.  [MGI Ref ID J:13874]

Additional References

Srebf2^lop13 related

Chang B; Hawes NL; Hurd RE; Wang J; Howell D; Davisson MT; Roderick TH; Nusinowitz S; Heckenlively JR. 2005. Mouse models of ocular diseases. Vis Neurosci 22(5):587-93. [PubMed: 16332269]  [MGI Ref ID J:156373]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Eye Mutant Resource within the Mouse Mutant Resource collection.

Control Information

	Control
	None Available
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.