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Strain Name:

B6C3Fe a/a-Unc5crcm/J

Stock Number:

001607

Availability:

Repository-Cryopreserved

Price and Supply Information

General Terms and Conditions

Former Name      B6C3Fe a/a-Unc5h3rcm/J    (Changed: 15-DEC-04 )
      B6C3Fe-a/a-Unc5h3rcm    (Changed: 15-DEC-04 )
Genes & Alleles   Unc5c;   Unc5crcm;   a;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Spontaneous Mutation
Specieslaboratory mouse
GenerationN3

Strain Description
The cerebellum of mice homozygous for the rostral cerebellar malformation spontaneous mutation (Unc5crcm) is smaller with fewer folia, there are ectopic cerebellar cells in the midbrain, and abnormal neuronal migration. Homozygous mutant mice are ataxic and experience growth retardation early in life. Homozygous males usually do not breed.

Strain Development
The rostral cerebellar malformation mutation (rcm) arose spontaneously in the B6.C3- Pde6brd1 Hps4le/J stock recovered from cryopreservation at The Jackson Laboratory in 1989 at N40pF1. The stock was sibling mated for 2 generations then maintained by ovarian transplant-cross-intercross. The first cross used ovaries from a homozygous rcm female which were transplanted into a host that was then bred to a C57BL/6J male. The heterozygous offspring were intercrossed and an ovarian transplant was done from a homozygous female, then the host was bred with a C3HeB/FeJ-a/a male and their offspring intercrossed. Ovarian transplant was done from a resulting homozygyous female and the host was bred to B6C3Fe-a/a F1 which became the standard mate in the cross-intercross maintenance of this strain. In 1991 embryos were generated for cryopreservation by breeding N2 heterozygotes.

Mammalian Phenotype Terms assigned by genotype

Unc5crcm/Unc5crcm

        B6C3Fe a/a-Unc5crcm/J
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:1827)
    • males on this hybrid background can sire litters unlike males on an inbred C57BL/6J background
  • nervous system phenotype
  • abnormal cerebellar Purkinje cell layer (MGI Ref ID J:40243)
    • abnormal groups of migrating granule cells are associated with gaps in the Purkinje cell layer
    • abnormal Purkinje cell morphology (MGI Ref ID J:45577)
      • at E13.5 Purkinje cell progenitors extend slightly more rostrally towards the pontine area
      • ectopic Purkinje cell (MGI Ref ID J:40243)
        • in the inferior colliculus and tegmentum of the midbrain
        • ectopic cells are detected at P3 but not at P0
        • at E17.5 Purkinje cells invade the lateral regions of the pontine area
        • in the inferior colliculus and pontine area at P7
        • scattered randomly through the granule cell layer and cells are also seen in some areas of the molecular layer, all in the parafloccular lobe lateral to the midbrain
  • abnormal cerebellar granule layer (MGI Ref ID J:1827)
    • variable thickness and cell density in the parafloccular lobe lateral to the midbrain
    • throughout the cerebellum small tongue-like extensions of the molecular layer into the granule cell layer are seen
    • ectopic granule cells (MGI Ref ID J:40243)
      • an abnormal band of granule cells extends from the cerebellum into the inferior colliculus
      • ectopic cells are first detected at P3 and increase in number by P7
      • in the inferior colliculus and pontine area at P7
      • in some areas of the molecular layer in the parafloccular lobe lateral to the midbrain
  • abnormal cerebellar molecular layer (MGI Ref ID J:1827)
    • throughout the cerebellum small tongue-like extensions of the molecular layer into the granule cell layer are seen
  • abnormal cerebellum development (MGI Ref ID J:40243)
    • an abnormal band of granule cells extends from the cerebellum into the inferior colliculus
    • partial fusion of the inferior colliculus to the rostral cerebellum
    • expression analysis identifies neuroprogenitors ectopically located beneath the pia mater and at E17.5 these cells extend more rostrally than the Purkinje cell progenitors
    • ectopic cerebellar cells are seen in the inferior colliculus and pontine area at P7
    • abnormal cerebellar foliation (MGI Ref ID J:40243)
      • a reduction in size and number of folia is seen in midline sagital sections
    • abnormal cerebellar lobule formation (MGI Ref ID J:40243)
      • only 6 lobes are formed
      • vermal fissure formation is also abnormal in the lateral cerebral hemisphere
    • abnormal external granule cell layer morphology (MGI Ref ID J:40243)
      • abnormal external granule cell migration with cells migrating in cohorts rather than as single cells
      • these abnormal groups of cells are often associated with gaps in the Purkinje cell layer
  • abnormal inferior colliculus (MGI Ref ID J:40243)
    • an abnormal band of granule cells extends from the cerebellum into the inferior colliculus
    • partial fusion of the inferior colliculus to the rostral cerebellum with ectopic granule and Purkinje cells in the inferior colliculuc and tegmentum of the midbrain
  • abnormal neuronal migration (MGI Ref ID J:45577)
    • abnormal rostral migration of Purkinje and granule cells into the inferior colliculus
  • small cerebellum (MGI Ref ID J:40243)
    • less pronounced than in Unc5crcmTg(Ucp)1.23Kz homozygotes
    • seen at E17.5
  • behavior/neurological phenotype
  • abnormal gait (MGI Ref ID J:1827)
    • swaying gait seen at 12 days of age
  • ataxia (MGI Ref ID J:1827)
  • growth/size phenotype
  • postnatal growth retardation (MGI Ref ID J:1827)
    • smaller than littermates at 12 days of age but catch up in size by 5 - 6 weeks of age

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Unc5crcm/Unc5crcm

        C57BL/6J-Unc5crcm
  • lethality-postnatal
  • postnatal lethality (MGI Ref ID J:1827)
    • fewer than expected homozygotes are found at weaning; however, those that survive to weaning have a normal life span
  • reproductive system phenotype
  • male infertility (MGI Ref ID J:14268)
    • males on an inbred C57BL/6J background fail to sire litters however, males on a hybrid B6C3Fe a/a background may sire litters
  • nervous system phenotype
  • abnormal cerebellum morphology (MGI Ref ID J:46670)
    • in lateral sections the cerebellum extends further anteriorly especially in the lateral vermal and paravermal regions
    • ectopic cerebellar tissue adheres to the posterior and lateral aspects of the inferior colliculus and along the lateral aspect of the brainstem
    • ectopic tissue is present paramedially but not at the midline and does not contain cerebellar nuclear cells
    • increased dispersal of cerebellar mossy fiber terminals and additional spinocerebellar mossy fiber terminal bands lateral to the usual 5 bands are seen in the anterior and ectopic cerebellar tissue; however mossy fibers in the posterior region appear similar to wild type
    • abnormal cerebellar cortex morphology (MGI Ref ID J:14268)
      • irregular thickness of the anterior lobe
      • abnormalities of the layers are more severe in the ectopic tissue
      • abnormal cerebellar Purkinje cell layer (MGI Ref ID J:14268)
        • Purkinje and granule cells are scrambled
        • increasing degree of multilamination as the normal anterior boundary of the cerebellum is approached
        • ectopic Purkinje cell (MGI Ref ID J:46670)
          • within the granule cell layer in the ectopic cerebellar tissue
      • abnormal cerebellar granule layer (MGI Ref ID J:14268)
        • Purkinje and granule cells are scrambled
        • acellular areas interrupt this layer
        • ectopic granule cells (MGI Ref ID J:46670)
          • in the medullary regions especially in the medial area of lobule VI
          • clusters of ectopic granule cells in the molecular layer in the ectopic cerebellar tissue
    • abnormal cerebellar lobule formation (MGI Ref ID J:46670)
      • smaller lobule V and less pronounced hemispheric part of lobule VI
      • lobule VI does not appear to extend to the lateral aspect of the hemisphere
      • lobule IX is reduced and appears to be joined to lobule VIII in the midline region
      • the medial cerebellum usually has only 2 lobules rather than 3 as in wild type mice
    • small cerebellum (MGI Ref ID J:46670)
  • increased inferior colliculus size (MGI Ref ID J:46670)
    • appears to extend further posteriorly
  • behavior/neurological phenotype
  • abnormal gait (MGI Ref ID J:1827)
    • swaying gait seen at 12 days of age
  • abnormal posture (MGI Ref ID J:46670)
    • broad stance of the hind limbs
    • when walking hind limbs and toes are splayed
  • ataxia (MGI Ref ID J:14268)
    • tend to fall to the side
    • tend to fall to one side, lean to one side when walking, and only rear when support is available
  • head bobbing (MGI Ref ID J:46670)
    • tend to bob up and down or side to side
  • tremors (MGI Ref ID J:46670)
    • slight
  • growth/size phenotype
  • postnatal growth retardation (MGI Ref ID J:1827)
    • smaller than littermates at 12 days of age but catch up in size by 5 - 6 weeks of age
  • hearing/vestibular/ear phenotype
  • head bobbing (MGI Ref ID J:46670)
    • tend to bob up and down or side to side

Gene & Allele Details

Allele Symbol Unc5crcm
Allele Name rostral cerebellar malformation
Common Name(s) rcm; rcms;
Strain of OriginC57BL/6J-Hps4
Gene Symbol and Name Unc5c, unc-5 homolog C (C. elegans)
Chromosome 3
Gene Common Name(s) AI047720; B130051O18Rik; RIKEN cDNA B130051O18 gene; UNC-5 homolog (C. elegans) 3; UNC5H3; Unc5h3; expressed sequence AI047720; rcm; rostral cerebellar malformation; unc5 homolog (C. elegans) 3;
Molecular Note The mutation underlying the mutant phenotype is a tandem duplication of an exon encoding amino acids 763-818. The transcript expressed from this allele contains an in-frame insertion that is predicted to result in a protein that contains an additional 55 amino acids in the cytoplasmic region. [MGI Ref ID J:40243]
 
Allele Symbol a
Allele Name nonagouti

Control Information

  Allele   Control
 Unc5crcm  Untyped from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Related Strains

Strains carrying   a allele
003879   B10;TFLe-a/a T tf/+ tf/J
001538   B6 x B6C3Sn a/A-T(1;9)27H/J
000916   B6 x B6C3Sn a/A-T(5;12)31H/J
000602   B6 x B6C3Sn a/A-T(8;16)17H/J
000618   B6 x FSB/GnEi a/a Ctslfs/J
000577   B6 x STOCK a Oca2p Hps5ru2 Ednrbs/J
000601   B6 x STOCK a/a T(7;18)50H/J
000592   B6 x STOCK T(2;4)13H a/J
000001   B6.C3 A/a Mgrn1md/J
000785   B6;D2-a Es1e/J
000604   B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J
002807   B6C3Fe a/a-Meox2fla/J
000224   B6C3Fe a/a-Scyl1mdf/J
001037   B6C3Fe a/a-Agtpbp1pcd/J
000221   B6C3Fe a/a-Alx4lst-J/J
002062   B6C3Fe a/a-Atp7aMo-8J/J
001756   B6C3Fe a/a-Cacng2stg/J
001815   B6C3Fe a/a-Col1a2oim/J
000231   B6C3Fe a/a-Csf1op/J
000209   B6C3Fe a/a-Dh/J
000211   B6C3Fe a/a-Dstdt-J/J
000210   B6C3Fe a/a-Edardl-J/J
000207   B6C3Fe a/a-Edaraddcr/J
000182   B6C3Fe a/a-Eef1a2wst/J
001278   B6C3Fe a/a-Glra1spd/J
000241   B6C3Fe a/a-Glrbspa/J
002875   B6C3Fe a/a-Hoxd13spdh/J
000304   B6C3Fe a/a-Krt71Ca Scn8amed-J/J
000226   B6C3Fe a/a-Largemyd/J
000636   B6C3Fe a/a-Lmx1adr-J/J
001280   B6C3Fe a/a-Lse/J
001573   B6C3Fe a/a-MitfMi/J
001035   B6C3Fe a/a-Napahyh/J
000181   B6C3Fe a/a-Otogtwt/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000205   B6C3Fe a/a-Papss2bm/J
002078   B6C3Fe a/a-Pcdh15av-2J/J
000246   B6C3Fe a/a-Pitpnavb/J
001430   B6C3Fe a/a-Ptch1mes/J
000506   B6C3Fe a/a-Qkqk/J
000235   B6C3Fe a/a-Relnrl/J
000237   B6C3Fe a/a-Rorasg/J
000290   B6C3Fe a/a-Sox10Dom/J
000230   B6C3Fe a/a-Tcirg1oc/J
003612   B6C3Fe a/a-Trak1hyrt/J
001512   B6C3Fe a/a-Ttnmdm/J
000005   B6C3Fe a/a-Wc/J
000243   B6C3Fe a/a-Wnt1sw/J
000248   B6C3Fe a/a-Xpl/J
001750   B6C3Fe a/a-XsJ/J
000624   B6C3Fe a/a-anx/J
003020   B6C3Fe a/a-dep/J
002018   B6C3Fe a/a-din/J
002339   B6C3Fe a/a-nma/J
000240   B6C3Fe a/a-soc/J
000063   B6C3Fe a/a-sy/J
001055   B6C3Fe a/a-tip/J
000245   B6C3Fe a/a-tn/J
000296   B6C3Fe-a/a Hoxa13Hd Mcoln3Va-J/J
000019   B6C3Fe-a/a-Itpr1opt/J
001022   B6C3FeF1/J a/a
000971   B6EiC3 a/A-Och/J
000551   B6EiC3 a/A-Tbx15de-H/J
006450   B6EiC3 a/A-Vss/J
000557   B6EiC3-+ a/LnpUl A/J
000503   B6EiC3Sn a/A-Gy/J
001811   B6EiC3Sn a/A-Otcspf-ash/J
002343   B6EiC3Sn a/A-Otcspf/J
000391   B6EiC3Sn a/A-Pax6Sey-Dey/J
001924   B6EiC3Sn a/A-Ts(1716)65Dn
001923   B6EiC3Sn a/A-Ts(417)2Lws Tim/J
000225   C3FeLe.B6 a/a-Ptpn6me/J
000198   C3FeLe.B6-a/J
000291   C3FeLe.Cg-a/a Hm KitlSl Krt71Ca-J/J
001886   C3HeB/FeJLe a/a-gnd/J
000584   C57BL/6J-+ T(1;2)5Ca/a +/J
000284   CWD/LeJ
000670   DBA/1J
000671   DBA/2J
001057   HPT/LeJ
000260   JGBF/LeJ
000265   MY/HuLeJ
000308   SSL/LeJ
000994   STOCK a Myo5ad Mregdsu/J
000064   STOCK a Tyrp1b Sisi/J
002238   STOCK a Tyrp1b shmy/J
001433   STOCK a skt/J
000579   STOCK a tp/J
000319   STOCK a us/J
002648   STOCK a/a Cln6nclf/J
000317   STOCK a/a Egfrwa2/J
000302   STOCK a/a MitfMi-wh +/+ Itpr1opt/J
000286   STOCK a/a Myo5ad fd/+ +/J
000206   STOCK a/a Tyrc-h/J
001432   STOCK a/a Tyrp1b sks/Tyrp1b +/J
000281   STOCK a/a ma ft/ma ft/J
000312   STOCK stb + a/+ Fignfi a/J
000596   STOCK T(2;11)30H/+ x AEJ-a Gdf5bp-H/J or A/J-a Gdf5bp-J/J
000970   STOCK T(2;16)28H A/T(2;16)28H a/J
000590   STOCK T(2;4)1Sn a/J
000594   STOCK T(2;8)26H a/T(2;8)26H a Tyrp1+/Tyrp1b/J
000623   TR/DiEiJ
View Strains carrying   a     (102 strains)

Strains carrying other alleles of a
003301   (C57BL/6J x C3H-Eya1bor)F1/J
000251   AEJ.Cg-ae +/a Gdf5bp-H/J
000202   AEJ/Gn-bd/J
000199   AEJ/GnLeJ
000427   B10.CE-H13b Aw/(30NX)SnJ
000420   B10.LP-H13b Aw/Sn
000477   B10.PA-Pldnpa H3e at/SnJ
000419   B10.UW-H3b we Pax1un at/SnJ
000593   B6 x B6CBCa Aw-J/A-Grid2Lc T(2;6)7Ca MitfMi-wh/J
000502   B6 x B6CBCa Aw-J/A-Myo5aflr Gnb5flr/J
000599   B6 x B6CBCa Aw-J/A-T(5;13)264Ca KitW-v/J
002083   B6 x B6EiC3 a/A-T(7;16)235Dn/J
000507   B6 x B6EiC3 a/A-Otcspf/J
002016   B6(Cg)-Aw-J EdaTa-6J Chr YB6-Sxr/EiJ
000552   B6-Aw-J-EdaTa-6J.Cg-Sxr
001730   B6-Aw-J-EdaTa-6J.Cg-Sxrb Hya-/J
000841   B6-Aw-J.CBy-EdaTa-By/J
001809   B6-Aw-J.Cg-EdaTa-6J +/+ ArTfm/J
000600   B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
000769   B6.C/(HZ18)By-at-44J/J
000203   B6.C3-Aiy/a/J
000017   B6.C3Fe-Avy/J
000628   B6.CE-A Amy1b Amy2b/J
005505   B6.Cg-Ay Slc7a11sut/LmLlp
000021   B6.Cg-Ay/J
001572   B6.Cg-am-J/J
100409   B6129PF1/J-Aw-J/Aw
004200   B6;CBACa Aw-J/A-Npr2cn-2J/J
000505   B6C3 Aw-J/A-Mutedmu/J
000604   B6C3 a/A-T(10;13)199H +/+ Lystbg-J/J or Lystbg-2J/J
000065   B6C3Fe a/a-we Pax1un at/J
000314   B6CBACa Aw-J/A-EdaTa/J-XO
000501   B6CBACa Aw-J/A-Aifm1Hq/J
001046   B6CBACa Aw-J/A-Grid2Lc/J
000500   B6CBACa Aw-J/A-Gs/J
002703   B6CBACa Aw-J/A-Hydinhy3/J
000247   B6CBACa Aw-J/A-Kcnj6wv/J
000287   B6CBACa Aw-J/A-Plp1jp EdaTa/J
000515   B6CBACa Aw-J/A-SfnEr/J
000242   B6CBACa Aw-J/A-spc/J
000288   B6CBACa Aw-J/A-we a Mafbkr/J
001201   B6CBACaF1/J-Aw-J/A
001752   B6CBCa Aw-J/A-T(7;15)9H/J
006450   B6EiC3 a/A-Vss/J
000557   B6EiC3-+ a/LnpUl A/J
000504   B6EiC3Sn a/A-Cacnb4lh/J
000553   B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J
001811   B6EiC3Sn a/A-Otcspf-ash/J
002343   B6EiC3Sn a/A-Otcspf/J
001923   B6EiC3Sn a/A-Ts(417)2Lws Tim/J
000200   C3FeB6 A/Aw-J-Ankank/J
000638   C3FeB6 A/Aw-J-Spnb4qv-J/J
001203   C3FeB6F1/J A/Aw-J
001272   C3H/HeSnJ-Ahvy/J
000099   C3HeB/FeJ-Avy/J
000338   C57BL/6J Aw-J-EdaTa-6J/J
000258   C57BL/6J-Ai/a/J
000774   C57BL/6J-Asy/a/J
000569   C57BL/6J-Aw-J-EdaTa +/+ ArTfm/J
000051   C57BL/6J-Aw-J/J
000055   C57BL/6J-at-33J/J
000070   C57BL/6J-atd/J
002468   KK.Cg-Ay/J
000262   LS/LeJ
000283   LT.CAST-A/J
001759   STOCK A Tyrc Sha/J
001427   STOCK Aw us/J
View Strains carrying other alleles of a     (67 strains)

Research Applications

This mouse can be used to support research in many areas including:

Unc5crcm related

Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Receptor Defects

References

Additional References

Price and Supply Information

Strain Name: B6C3Fe a/a-Unc5crcm/J
Stock Number: 001607

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)
Cryorecovery Fee $2470.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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