Description

Strain Information

Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse and M. m. castaneus Generation N36 (08-JAN-04)

Appearance
black
Related Genotype: a/a

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying   Gpi1^a allele

000600	B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
000377	B6.C-H22c Gpi1a/(HW38)ByJ
000123	B6.C-H24c Gpi1a/(HW54)ByJ
000797	B6.CAST-Gpi1a/EiJ
002074	B6.Cg-Gpi1a Hbbd H1b/DehJ
001317	B6.Cg-Igha Thy1a Gpi1a/J
000909	B6CBy-Gpi1a/Gpi1b/J or Gpi1a-m1/Gpi1b/J
002800	C3.Cg-Gpi1a/J

View Strains carrying   Gpi1^a     (8 strains)

Strains carrying   Hba^th-J allele

000802

WB.Cg-Hbath-J/J

View Strains carrying   Hba^th-J     (1 strain)

Strains carrying other alleles of Gpi1

005730	129S6.CB(B6)-Del(1)1Brk Gpi1b/Gpi1c/BrkMdfJ
002027	129S8/SvEv-Gpi1c Hprt1b-m2/J
000600	B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
006561	B6.CBy(Cg)-Gpi1a-m1J/BrkJ
006562	B6.CBy(Cg)-Gusbmps Gpi1a-m1J/BrkJ
000909	B6CBy-Gpi1a/Gpi1b/J or Gpi1a-m1/Gpi1b/J
006560	CBy(Cg)-Gpi1a-m1J/BrkJ
003585	NOD.B6-(Gpi1-D7Mit346)/LtJ

View Strains carrying other alleles of Gpi1     (8 strains)

Strains carrying other alleles of Hba

002616	B6.129S7-Hbatm1Paz/J
003342	STOCK Hbatm1Paz Hbbtm1Tow Tg(HBA-HBBs)41Paz/J

View Strains carrying other alleles of Hba     (2 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Thalassemias -

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Hba^th-J/Hba⁺

        involves: C57BL/6J

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:32654)
  • fewer than expected heterozygous mice are found at weaning (39% compared to the expected 50%)
• growth/size phenotype
• decreased body weight (MGI Ref ID J:45721)
• hematopoietic system phenotype
• decreased hemoglobin content (MGI Ref ID J:45721)
  • low blood hemoglobin levels
• increased erythrocyte cell number (MGI Ref ID J:45721)
  • erythrocytosis
• microcytosis (MGI Ref ID J:45721)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Hematological Research
Hemoglobin Defects (thalassemia)

Gpi1^a related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research (Tissue/Cell Markers)

Hba^th-J related

Developmental Biology Research
Embryonic Lethality (Homozygous)

Hematological Research
Hemoglobin Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Gpi1a
Allele Name		a variant
Allele Type		Not Applicable
Gene Symbol and Name		Gpi1, glucose phosphate isomerase 1
Chromosome		7
Gene Common Name(s)		AMF; GNPI; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; MF; NK; NK/GPI; NLK; Org; PGI; PHI; SA-36; autocrine motility factor; glucose phosphate isomerase 1, regulatory; glucose phosphate isomerase 1, structural; glucose phosphate isomerase 1, temporal; maturation factor; neuroleukin;
Molecular Note		This allele determines a slower migrating variant of the encoded enzyme and occurs in the wild and in strains A/HeJ, AKR/J, BALB/cJ, C57BR/cdJ, C58/J, CE/J, DBA/2J, DE/J, DW/J, LP/J, MA/J, NZB, PL/J, P/J, RARF, RF/J, SJL/J, ST/bJ, and 129/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1b. Two differences were silent, while a third difference at position 282 predicts an aspartate for the a allele and an asparagine for the b allele. A fourth difference in the 3' untranslated region was also noted. [MGI Ref ID J:28033]
Allele Symbol		Hbath-J
Allele Name		alpha thalassemia Jackson
Allele Type		Chemically induced (other)
Common Name(s)		alphaMD;
Strain of Origin		C57BL/6J x Triethyllenemelamine-treated male
Gene Symbol and Name		Hba, hemoglobin alpha chain complex
Chromosome		11
General Note		The mutation was induced in a male mouse by triethyllenemelamine.
Molecular Note		This mutation was shown to be a deletion that included the Hba-x, Hba-a1 and Hba-a2 genes (J:32654). The Mpg, Rhbdf1 and Phg genes are also included in the deletion region (J:29665). [MGI Ref ID J:29665] [MGI Ref ID J:32654]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Gpi1^a related

Charles DJ; Lee CY. 1980. Biochemical and immunological characterization of genetic variants of phosphoglucose isomerase from mouse. Biochem Genet 18(1-2):153-69. [PubMed: 6155905]  [MGI Ref ID J:6330]

Chen J; Reifsnyder PC; Scheuplein F; Schott WH; Mileikovsky M; Soodeen-Karamath S; Nagy A; Dosch MH; Ellis J; Koch-Nolte F; Leiter EH. 2005. 'Agouti NOD': identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells. Mamm Genome 16(10):775-83. [PubMed: 16261419]  [MGI Ref ID J:102639]

Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866]  [MGI Ref ID J:107466]

Collinson JM; Quinn JC; Hill RE; West JD. 2003. The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo. Dev Biol 255(2):303-12. [PubMed: 12648492]  [MGI Ref ID J:82632]

DeLorenzo RJ; Ruddle FH. 1969. Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem Genet 3(2):151-62. [PubMed: 5364923]  [MGI Ref ID J:5141]

Ito CY; Li CY; Bernstein A; Dick JE; Stanford WL. 2003. Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice. Blood 101(2):517-23. [PubMed: 12393491]  [MGI Ref ID J:81387]

Pearce SR; Morgan MJ; Ball S; Peters J; Faik P. 1995. Sequence characterization of alleles Gpi1-Sa and Gpi1-Sb at the glucose phosphate isomerase structural locus. Mamm Genome 6(8):537-9. [PubMed: 8589524]  [MGI Ref ID J:28033]

Szuber N; Buss JL; Soe-Lin S; Felfly H; Trudel M; Ponka P. 2008. Alternative treatment paradigm for thalassemia using iron chelators. Exp Hematol 36(7):773-85. [PubMed: 18456387]  [MGI Ref ID J:136991]

Hba^th-J related

Chang J; Lu RH; Xu SM; Meneses J; Chan K; Pedersen R; Kan YW. 1996. Inactivation of mouse alpha-globin gene by homologous recombination: mouse model of hemoglobin H disease. Blood 88(5):1846-51. [PubMed: 8781443]  [MGI Ref ID J:35018]

Fabry ME; Costantini F; Pachnis A; Suzuka SM; Bank N; Aynedjian HS; Factor SM; Nagel RL. 1992. High expression of human beta S- and alpha-globins in transgenic mice: erythrocyte abnormalities, organ damage, and the effect of hypoxia. Proc Natl Acad Sci U S A 89(24):12155-9. [PubMed: 1465455]  [MGI Ref ID J:129537]

Fabry ME; Nagel RL; Pachnis A; Suzuka SM; Costantini F. 1992. High expression of human beta S- and alpha-globins in transgenic mice: hemoglobin composition and hematological consequences. Proc Natl Acad Sci U S A 89(24):12150-4. [PubMed: 1465454]  [MGI Ref ID J:129579]

Fabry ME; Sengupta A; Suzuka SM; Costantini F; Rubin EM; Hofrichter J; Christoph G; Manci E; Culberson D; Factor SM; et al.. 1995. A second generation transgenic mouse model expressing both hemoglobin S (HbS) and HbS-Antilles results in increased phenotypic severity. Blood 86(6):2419-28. [PubMed: 7662990]  [MGI Ref ID J:94190]

Hendrey J; Lin D; Dziadek M. 1995. Developmental analysis of the Hba(th-J) mouse mutation: effects on mouse peri-implantation development and identification of two candidate genes. Dev Biol 172(1):253-63. [PubMed: 7589805]  [MGI Ref ID J:29665]

Hendrey JA; Dziadek M. 1995. Effects on neonatal growth of the Hba(th-j) deletion on mouse chromosome 11 are not due to genomic imprinting. Growth Regul 5(3):160-3. [PubMed: 7580867]  [MGI Ref ID J:29127]

Whitney JB 3d; Martinell J; Popp RA; Russell LB; Anderson WF. 1981. Deletions in the alpha-globin gene complex in alpha-thalassemic mice. Proc Natl Acad Sci U S A 78(12):7644-7. [PubMed: 6950407]  [MGI Ref ID J:32654]

Whitney JB 3d; Popp RA. 1984. Animal model of human disease: thalassemia: alpha-thalassemia in laboratory mice. Am J Pathol 116(3):523-5. [PubMed: 6476083]  [MGI Ref ID J:7581]

Whitney JB 3d; Russell ES. 1980. Linkage of genes for adult alpha-globin and embryonic alpha-like globin chains. Proc Natl Acad Sci U S A 77(2):1087-90. [PubMed: 6153802]  [MGI Ref ID J:6282]

Whitney JB; Russell ES. 1978. New mutants and biochemical variants: Alpha thalassemia Mouse News Lett 58:47-48.  [MGI Ref ID J:45721]

Yu X; Kong Y; Dore LC; Abdulmalik O; Katein AM; Zhou S; Choi JK; Gell D; Mackay JP; Gow AJ; Weiss MJ. 2007. An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis. J Clin Invest 117(7):1856-65. [PubMed: 17607360]  [MGI Ref ID J:124209]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.