Description

Strain Information

Former Names AXB-6/PgnJ    (Changed: 15-DEC-04 ) Type Recombinant Inbred (Ri); Additional information on Recombinant Inbred Mice. Mating System Sibling x Sibling         (Female x Male) Species laboratory mouse RI progenitor A/J C57BL/6J H2 Haplotype b Generation F85+18 (04-NOV-08)

Appearance
albino
Related Genotype: a/a Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c

Important Note
This strain is homozygous/hemizygous for Cacna1f^nob2, no b wave 2, which results in impaired light- and dark-adapted electroretinogram responses.

Description
The AXB and BXA set of RI strains are useful in the genetic analysis of several complex diseases including cardiovascular disease, diabetes, cancer, cleft palate, and hydrocephalus. The individual strains within the RI set also differ in their susceptibility to infectious diseases and in their responses to alcohol, stress, and endotoxin. The strain distribution pattern (SDP) for both the AXB and BXA RI strains is available through the Mouse Genome Informatics Recombinant Inbred Strain Distribution Patterns Query Form

Development
The AXB and BXA recombinant inbred (RI) set of strains were originally generated and maintained at the University of California, San Diego, and have been imported into The Jackson Laboratory. There were originally 51 AXB and BXA strains derived from the C57BL/6J (Stock No. 000664) and A/J (Stock No. 000646) progenitor strains. Many of the original strains are either extinct or were discarded due to genetic contamination. Please refer to Sampson et al., 1998 for more information.

Related Strains

AXB Strains

001673	AXB1/PgnJ
001681	AXB10/PgnJ
001683	AXB12/PgnJ
001826	AXB13/PgnJ
001684	AXB13a/PgnJ
001685	AXB15/PgnJ
001687	AXB19/PgnJ
001686	AXB19a/PgnJ
001688	AXB19b/PgnJ
001674	AXB2/PgnJ
001690	AXB23/PgnJ
001691	AXB24/PgnJ
001676	AXB4/PgnJ
001677	AXB5/PgnJ
001679	AXB8/PgnJ

View AXB Strains     (15 strains)

Additional Web Information

JAX^® NOTES, Spring 1996; 465. AXB/BXA Recombinant Inbred Strains.

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Mouse Phenome Database
Wellcome Trust Centre for Human Genetics: Mouse Recombinant Inbred Line (RIL) Genotype Data for AXB RI Line

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects (genes regulating preferences to alcohol and stress)

Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Genetics Research (Gene Mapping: Tools for QTL Mapping, Segregation and Linkage Analysis)
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)

Sensorineural Research
Eye Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cacna1fnob2
Allele Name		no b wave 2
Allele Type		Spontaneous
Common Name(s)		Cav1.4nob2; nob2;
Strain of Origin		AXB6/PgnJ
Gene Symbol and Name		Cacna1f, calcium channel, voltage-dependent, alpha 1F subunit
Chromosome		X
Gene Common Name(s)		AIED; COD3; CORDX; CORDX3; CSNB2; CSNB2A; CSNBX2; Cav1.4; JM8; JMC8; OA2; Sfc17; no b wave 2; nob2;
Molecular Note		Sequence analysis identifies this allele as an insertion of a transposable element in exon 2. The out-of-frame insertion produces a stop codon after synthesis of 32 amino acids. Immunofluorescent staining using an antibody directed to the C terminal of the subunit protein indicates that it is not expressed in the inner or outer plexiform layers of the retina. [MGI Ref ID J:123811]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Brodkin ES; Carlezon WA Jr; Haile CN; Kosten TA; Heninger GR ; Nestler EJ. 1998. Genetic analysis of behavioral, neuroendocrine, and biochemical parameters in inbred rodents: initial studies in Lewis and Fischer 344 rats and in A/J and C57BL/6J mice. Brain Res 805(1-2):55-68. [PubMed: 9733917]  [MGI Ref ID J:50636]

Gill K; Desaulniers N; Desjardins P; Lake K. 1998. Alcohol preference in AXB/BXA recombinant inbred mice: gender differences and gender-specific quantitative trait loci. Mamm Genome 9(12):929-35. [PubMed: 9880655]  [MGI Ref ID J:52467]

Marshall JD; Mu JL; Cheah YC; Nesbitt MN; Frankel WN; Paigen B. 1992. The AXB and BXA set of recombinant inbred mouse strains. Mamm Genome 3(12):669-80. [PubMed: 1477475]  [MGI Ref ID J:3516]

Mu JL; Naggert JK; Nishina PM; Cheah YC; Paigen B. 1993. Strain distribution pattern in AXB and BXA recombinant inbred strains for loci on murine chromosomes 10, 13, 17, and 18. Mamm Genome 4(3):148-52. [PubMed: 8094988]  [MGI Ref ID J:4133]

Sampson SB; Higgins DC; Elliot RW; Taylor BA; Lueders KK; Koza RA; Paigen B. 1998. An edited linkage map for the AXB and BXA recombinant inbred mouse strains. Mamm Genome 9(9):688-94. [PubMed: 9716653]  [MGI Ref ID J:45463]

Cacna1f^nob2 related

Chang B; Heckenlively JR; Bayley PR; Brecha NC; Davisson MT; Hawes NL; Hirano AA; Hurd RE; Ikeda A; Johnson BA; McCall MA; Morgans CW; Nusinowitz S; Peachey NS; Rice DS; Vessey KA; Gregg RG. 2006. The nob2 mouse, a null mutation in Cacna1f: anatomical and functional abnormalities in the outer retina and their consequences on ganglion cell visual responses. Vis Neurosci 23(1):11-24. [PubMed: 16597347]  [MGI Ref ID J:123811]

Doering CJ; Rehak R; Bonfield S; Peloquin JB; Stell WK; Mema SC; Sauve Y; McRory JE. 2008. Modified Ca(v)1.4 expression in the Cacna1f(nob2) mouse due to alternative splicing of an ETn inserted in exon 2. PLoS ONE 3(7):e2538. [PubMed: 18596967]  [MGI Ref ID J:137986]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Mating System	Sibling x Sibling         (Female x Male)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and six weeks of age. This strain is included in the Special Mutant Stock Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note	This strain is homozygous/hemizygous for Cacna1fnob2, no b wave 2, which results in impaired light- and dark-adapted electroretinogram responses.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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