Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Tg(L-FABP, S-GH)10Bir/J    (Changed: 04-AUG-08 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F5+N1p

Related Strains

Strains carrying other alleles of Fabp1

001400	STOCK Tg(Fabp1-GH1)5Bir/J
001515	STOCK Tg(Fabp1-GH1)7Bir/J

View Strains carrying other alleles of Fabp1     (2 strains)

Strains carrying other alleles of GH1

006086	B6.SJL-Tg(HBB-GH1)420King/J
005282	NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
001400	STOCK Tg(Fabp1-GH1)5Bir/J
001515	STOCK Tg(Fabp1-GH1)7Bir/J

View Strains carrying other alleles of GH1     (4 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation

Developmental Biology Research
Growth Defects
Internal/Organ Defects

Endocrine Deficiency Research
Gastrointestinal Defects

Internal/Organ Research
Gastrointestinal Defects

Research Tools
Developmental Biology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Fabp1-GH1)10Bir
Allele Name		transgene insertion 10, Edward H Birkenmeier
Allele Type		Transgenic (random, expressed)
Common Name(s)		L-FABP(-596 to +21)/hGH transgene; Tg(L-FABP, S-GH)10Bir; short promoter L-FABP-hGH transgene;
Strain of Origin		(C57BL/6J x LT/Sv)F1
Expressed Gene		GH1, growth hormone 1, human
Promoter		Fabp1, fatty acid binding protein 1, liver, mouse, laboratory
Molecular Note		This transgene comprises a human growth hormone gene whose 5' regulatory elements have been replaced by nucleotides -596 to +21 from the rat liver fatty-acid binding protein gene. Mice of this line (from founder G0#19) have ~800 transgene copies per haploid genome, as measured by densitometric analysis of Southern blots. Expression of human growth hormone mRNA, determined by dot-blot analysis, correlates generally with that of endogenous mouse Fabp1 mRNA in liver and intestine, including a proximal-to-distal declining expression gradient in the small intestine (with somewhat higher hGH expression in the ileum); neither is expressed in several other tissues examined, but hGH, unlike Fabp1, is expressed in cecum, colon and kidneys of transgenic mice. This"ectopic" expression is suppressed by more 5' regulatory sequences present in similar transgenes with a longer Fabp1 promoter (See Tg(Fabp1-GH1)5Bir). [MGI Ref ID J:109939]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Roth KA; Rubin DC; Birkenmeier EH; Gordon JI. 1991. Expression of liver fatty acid-binding protein/human growth hormone fusion genes within the enterocyte and enteroendocrine cell populations of fetal transgenic mice. J Biol Chem 266(9):5949-54. [PubMed: 1706343]  [MGI Ref ID J:134931]

Additional References

Tg(Fabp1-GH1)10Bir related

Cohn SM; Roth KA; Birkenmeier EH; Gordon JI. 1991. Temporal and spatial patterns of transgene expression in aging adult mice provide insights about the origins, organization, and differentiation of the intestinal epithelium. Proc Natl Acad Sci U S A 88(3):1034-8. [PubMed: 1992454]  [MGI Ref ID J:134930]

Hauft SM; Sweetser DA; Rotwein PS; Lajara R; Hoppe PC; Birkenmeier EH; Gordon JI. 1989. A transgenic mouse model that is useful for analyzing cellular and geographic differentiation of the intestine during fetal development. J Biol Chem 264(14):8419-29. [PubMed: 2656678]  [MGI Ref ID J:134929]

Sweetser DA; Birkenmeier EH; Hoppe PC; McKeel DW; Gordon JI. 1988. Mechanisms underlying generation of gradients in gene expression within the intestine: an analysis using transgenic mice containing fatty acid binding protein-human growth hormone fusion genes. Genes Dev 2(10):1318-32. [PubMed: 2462524]  [MGI Ref ID J:109939]

Trahair JF; Neutra MR; Gordon JI. 1989. Use of transgenic mice to study the routing of secretory proteins in intestinal epithelial cells: analysis of human growth hormone compartmentalization as a function of cell type and differentiation. J Cell Biol 109(6 Pt 2):3231-42. [PubMed: 2689454]  [MGI Ref ID J:137510]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.