Strain Name:

B6.MRL-Cacng2stg-wag/J

Stock Number:

001883

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6J-Cacng2stg-wag/J    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse

Development
The waggler mutation arose spontaneously on the MRL/MpJ-Tnfrsf6lpr background in the laboratory of Dr. Charles Sidman in 1988. This mutation was backcrossed to C57BL/6J via backcross-intercross and the lpr mutation was removed from the strain. Ovary transplantation from homozygous females was used when it became necessary. In 1991 N6 heterozygotes were bred together to generate embryos for cryopreservation.

Control Information

  Control
   Untyped from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cacng2
008624   B6.C-Cacng2stg-3J/LetJ
001756   B6C3Fe a/a-Cacng2stg/J
View Strains carrying other alleles of Cacng2     (2 strains)

Additional Web Information

JAX® NOTES, Spring 2005, 497. Supression of Absence Epilepsy Seizures Dependent on Complex Balance of Proteins.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Mental Retardation, Autosomal Dominant 10; MRD10   (CACNG2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Cacng2stg-wag/Cacng2stg-wag

        B6.MRL-Cacng2stg-wag/J
  • behavior/neurological phenotype
  • absence seizures
    • average spike wave dischrge duration was 1.8 +/- 1.5 seconds   (MGI Ref ID J:96506)
    • average number of spike wave discharges per hour was 18.7 +/- 40.9   (MGI Ref ID J:96506)
  • nervous system phenotype
  • absence seizures
    • average spike wave dischrge duration was 1.8 +/- 1.5 seconds   (MGI Ref ID J:96506)
    • average number of spike wave discharges per hour was 18.7 +/- 40.9   (MGI Ref ID J:96506)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cacng2stg-wag related

Cell Biology Research
Channel and Transporter Defects
      calcium

Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Channel and Transporter Defects
      calcium
Epilepsy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cacng2stg-wag
Allele Name waggler
Allele Type Spontaneous
Common Name(s) wag;
Strain of OriginMRL/MpJ-Faslpr/J
Gene Symbol and Name Cacng2, calcium channel, voltage-dependent, gamma subunit 2
Chromosome 15
Gene Common Name(s) AW060990; B230105C07Rik; B930041E13Rik; Ipr328; MRD10; RIKEN cDNA B230105C07 gene; RIKEN cDNA B930041E13 gene; TARP gamma 2; expressed sequence AW060990; stargazer; stargazin; stg; wag; waggler;
General Note Waggler is a second recessive mutation at the Cacng2 locus that arose in 1988 in the MRL/MpJ-Tnfrsf6 colony maintained at The Jackson Laboratory by Dr. Charles Sidman. Mutant homozygotes are characterized by whole body tremor, an unstable gait, and growth retardation. Hydrocephaly occurs occasionally. Hearing tests by Dr. Lawrence Erway of the University of Cinncinnati showed that the mutant was not deaf. Both sexes breed; no gross neuroanatomical or histopathologic lesions have been found (J:14313).
Molecular Note The phenotype of the waggler is associated with aberrant splicing residing in the first intron. Like the stargazer allele, a RT-PCR analysis demonstrated that in addition to aberrant transcripts, some normally spliced mRNA was detected, suggesting that this mutation does not represent a complete null allele. [MGI Ref ID J:48966]

Genotyping

Genotyping Information

Genotyping Protocols

Cacng2stg-wag, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Letts VA; Felix R; Biddlecome GH; Arikkath J; Mahaffey CL; Valenzuela A ; Bartlett FS 2nd ; Mori Y ; Campbell KP ; Frankel WN. 1998. The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit [see comments] Nat Genet 19(4):340-7. [PubMed: 9697694]  [MGI Ref ID J:48966]

Letts VA; Valenzuela A; Kirley JP; Sweet HO; Davisson MT; Frankel WN. 1997. Genetic and physical maps of the stargazer locus on mouse chromosome 15. Genomics 43(1):62-8. [PubMed: 9226373]  [MGI Ref ID J:38767]

Noebels JL; Qiao X; Bronson RT; Spencer C; Davisson MT. 1990. Stargazer: a new neurological mutant on chromosome 15 in the mouse with prolonged cortical seizures [published erratum appears in Epilepsy Res 1992 Mar;11(1):72] Epilepsy Res 7(2):129-35. [PubMed: 2289471]  [MGI Ref ID J:11008]

Cacng2stg-wag related

Bao S; Chen L; Qiao X; Knusel B; Thompson RF. 1998. Impaired eye-blink conditioning in waggler, a mutant mouse with cerebellar BDNF deficiency. Learn Mem 5(4-5):355-64. [PubMed: 10454360]  [MGI Ref ID J:51153]

Chen L; Bao S; Qiao X; Thompson RF. 1999. Impaired cerebellar synapse maturation in waggler, a mutant mouse with a disrupted neuronal calcium channel gamma subunit. Proc Natl Acad Sci U S A 96(21):12132-7. [PubMed: 10518588]  [MGI Ref ID J:58123]

Letts VA; Felix R; Biddlecome GH; Arikkath J; Mahaffey CL; Valenzuela A ; Bartlett FS 2nd ; Mori Y ; Campbell KP ; Frankel WN. 1998. The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit [see comments] Nat Genet 19(4):340-7. [PubMed: 9697694]  [MGI Ref ID J:48966]

Letts VA; Kang MG; Mahaffey CL; Beyer B; Tenbrink H; Campbell KP; Frankel WN. 2003. Phenotypic heterogeneity in the stargazin allelic series. Mamm Genome 14(8):506-13. [PubMed: 12925883]  [MGI Ref ID J:84624]

Letts VA; Mahaffey CL; Beyer B; Frankel WN. 2005. A targeted mutation in Cacng4 exacerbates spike-wave seizures in stargazer (Cacng2) mice. Proc Natl Acad Sci U S A 102(6):2123-8. [PubMed: 15677329]  [MGI Ref ID J:96506]

Sweet HO. 1993. Waggler and stargazer are allelic Mouse Genome 91:312-3.  [MGI Ref ID J:30739]

Sweet HO; Bronson RT; Cook S; Spencer CA; Davisson MT. 1991. Waggler (wag). Mouse Genome 89:552-53.  [MGI Ref ID J:14311]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery of Strains Needing Progeny Testing
    At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation.
    Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation.

    Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Untyped from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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