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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6J-Cacng2stg-wag/J (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7
Generation DefinitionsDevelopment
The waggler mutation arose spontaneously on the MRL/MpJ-Tnfrsf6lpr background in the laboratory of Dr. Charles Sidman in 1988. This mutation was backcrossed to C57BL/6J via backcross-intercross and the lpr mutation was removed from the strain. Ovary transplantation from homozygous females was used when it became necessary. In 1991 N6 heterozygotes were bred together to generate embryos for cryopreservation.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Cacng2
008624 B6.C-Cacng2stg-3J/LetJ 001756 B6C3Fe a/a-Cacng2stg/J View Strains carrying other alleles of Cacng2 (2 strains)
Additional Web Information
JAX® NOTES, Spring 2005, 497. Supression of Absence Epilepsy Seizures Dependent on Complex Balance of Proteins.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Mental Retardation, Autosomal Dominant 10; MRD10 (CACNG2)
assigned by genotype
Cacng2stg-wag/Cacng2stg-wag
B6.MRL-Cacng2stg-wag/J
- behavior/neurological phenotype
- absence seizures
- nervous system phenotype
- absence seizures
This mouse can be used to support research in many areas including:Cacng2stg-wag related
Cell Biology Research
Channel and Transporter Defects
calcium
Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Channel and Transporter Defects
calcium
Epilepsy
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Cacng2stg-wag | ||
|---|---|---|---|
| Allele Name | waggler | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | wag; | ||
| Strain of Origin | MRL/MpJ-Faslpr/J | ||
| Gene Symbol and Name | Cacng2, calcium channel, voltage-dependent, gamma subunit 2 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | AW060990; B230105C07Rik; B930041E13Rik; Ipr328; MRD10; RIKEN cDNA B230105C07 gene; RIKEN cDNA B930041E13 gene; TARP gamma 2; expressed sequence AW060990; stargazer; stargazin; stg; wag; waggler; | ||
| General Note | Waggler is a second recessive mutation at the Cacng2 locus that arose in 1988 in the MRL/MpJ-Tnfrsf6 colony maintained at The Jackson Laboratory by Dr. Charles Sidman. Mutant homozygotes are characterized by whole body tremor, an unstable gait, and growth retardation. Hydrocephaly occurs occasionally. Hearing tests by Dr. Lawrence Erway of the University of Cinncinnati showed that the mutant was not deaf. Both sexes breed; no gross neuroanatomical or histopathologic lesions have been found (J:14313). | ||
| Molecular Note | The phenotype of the waggler is associated with aberrant splicing residing in the first intron. Like the stargazer allele, a RT-PCR analysis demonstrated that in addition to aberrant transcripts, some normally spliced mRNA was detected, suggesting that this mutation does not represent a complete null allele. [MGI Ref ID J:48966] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cacng2stg-wag, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Letts VA; Felix R; Biddlecome GH; Arikkath J; Mahaffey CL; Valenzuela A ; Bartlett FS 2nd ; Mori Y ; Campbell KP ; Frankel WN. 1998. The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit [see comments] Nat Genet 19(4):340-7. [PubMed: 9697694] [MGI Ref ID J:48966]
Letts VA; Valenzuela A; Kirley JP; Sweet HO; Davisson MT; Frankel WN. 1997. Genetic and physical maps of the stargazer locus on mouse chromosome 15. Genomics 43(1):62-8. [PubMed: 9226373] [MGI Ref ID J:38767]
Noebels JL; Qiao X; Bronson RT; Spencer C; Davisson MT. 1990. Stargazer: a new neurological mutant on chromosome 15 in the mouse with prolonged cortical seizures [published erratum appears in Epilepsy Res 1992 Mar;11(1):72] Epilepsy Res 7(2):129-35. [PubMed: 2289471] [MGI Ref ID J:11008]
Cacng2stg-wag relatedBao S; Chen L; Qiao X; Knusel B; Thompson RF. 1998. Impaired eye-blink conditioning in waggler, a mutant mouse with cerebellar BDNF deficiency. Learn Mem 5(4-5):355-64. [PubMed: 10454360] [MGI Ref ID J:51153]
Chen L; Bao S; Qiao X; Thompson RF. 1999. Impaired cerebellar synapse maturation in waggler, a mutant mouse with a disrupted neuronal calcium channel gamma subunit. Proc Natl Acad Sci U S A 96(21):12132-7. [PubMed: 10518588] [MGI Ref ID J:58123]
Letts VA; Felix R; Biddlecome GH; Arikkath J; Mahaffey CL; Valenzuela A ; Bartlett FS 2nd ; Mori Y ; Campbell KP ; Frankel WN. 1998. The mouse stargazer gene encodes a neuronal Ca2+-channel gamma subunit [see comments] Nat Genet 19(4):340-7. [PubMed: 9697694] [MGI Ref ID J:48966]
Letts VA; Kang MG; Mahaffey CL; Beyer B; Tenbrink H; Campbell KP; Frankel WN. 2003. Phenotypic heterogeneity in the stargazin allelic series. Mamm Genome 14(8):506-13. [PubMed: 12925883] [MGI Ref ID J:84624]
Letts VA; Mahaffey CL; Beyer B; Frankel WN. 2005. A targeted mutation in Cacng4 exacerbates spike-wave seizures in stargazer (Cacng2) mice. Proc Natl Acad Sci U S A 102(6):2123-8. [PubMed: 15677329] [MGI Ref ID J:96506]
Sweet HO. 1993. Waggler and stargazer are allelic Mouse Genome 91:312-3. [MGI Ref ID J:30739]
Sweet HO; Bronson RT; Cook S; Spencer CA; Davisson MT. 1991. Waggler (wag). Mouse Genome 89:552-53. [MGI Ref ID J:14311]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3000.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery of Strains Needing Progeny Testing.
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks.Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery of Strains Needing Progeny Testing.
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks.Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Untyped from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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