Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6-TgN(CETP)#Pnu    (Changed: 15-DEC-04 ) Type Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Beverly Paigen,   The Jackson Laboratory

Description
This transgenic strain overexpresses the simian plasma cholesteryl ester transfer protein (CETP). Homozygous transgenic are viable and fertile. Mice exhibit a negative correlation between plasma CETP activity and total plasma cholesterol, plasma apolipoprotein (apo) A-I levels, and plasma apo A-I to apo B ratio. The size of the apo A-I-containing lipoproteins is significantly reduced in mice with high plasma CETP activity. Transgenic mice on a high-fat, high-cholesterol diet have substantially higher plasma cholesterol and plasma apo B levels and the apo B-containing lipoproteins are generally larger than those in nontransgenic C57BL/6 mice on the same diet.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of CETP

003904

B6.CBA-Tg(CETP)5203Tall/J

View Strains carrying other alleles of CETP     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tg(CETP)UCTP20Pnu/0

        C57BL/6-Tg(CETP)UCTP20Pnu

• homeostasis/metabolism phenotype
• abnormal circulating cholesterol level
  • chow-fed mice have cholesterol levels about half that of male wild-type mice   (MGI Ref ID J:92448)
  • male mice fed a high fat, high cholesterol (HFHC) diet have a mean cholesterol level of 250 mg/dl compared to 163 mg/dl for male wild-type mice fed the same diet   (MGI Ref ID J:92448)
  • female mice fed a HFHC diet for 7 weeks have a mean cholesterol level of 191 mg/dl compared to 153 mg/dl for female wild-type mice fed the same diet   (MGI Ref ID J:92448)
  • chow fed mice have lower mean total cholesterol than control mice (61 mg/dl vs. 96 mg/dl   (MGI Ref ID J:118358)
  • the ratio of (VLDL + LDL) to HDL is three times higher than in wild-type mice   (MGI Ref ID J:118358)
  • mean total cholesterol levels are similar to the high levels in wild-type mice when both groups are fed a HFHC diet   (MGI Ref ID J:118358)
  • the ratio of (VLDL + LDL) to HDL remains two-three times higher on the HFHC diet   (MGI Ref ID J:118358)
• • decreased circulating HDL cholesterol level
    • HDL levels are half that found in wild-type mice when both groups are fed a chow diet   (MGI Ref ID J:118358)
    • HDL levels continue to be lower than wild-type mice when both groups are fed a HFHC diet   (MGI Ref ID J:118358)
  • increased circulating LDL cholesterol level
    • VLDL/LDL levels are a third higher in chow-fed mice compared to wild-type mice   (MGI Ref ID J:118358)
    • VLDL/LDL levels remain higher than in wild-type mice when both groups are fed a HFHC diet   (MGI Ref ID J:118358)
  • increased circulating VLDL cholesterol level
    • VLDL/LDL levels are a third higher in chow-fed mice compared to wild-type mice   (MGI Ref ID J:118358)
    • VLDL/LDL levels remain higher than in wild-type mice when both groups are fed a HFHC diet   (MGI Ref ID J:118358)
• abnormal circulating protein level
  • mice have higher blood levels of Apo-A-I and Apo-B compared to sex-matched control mice   (MGI Ref ID J:92448)
  • the levels of Apo-B but not Apo-A-I increase to a greater extent than controls when mice are fed a high fat, high cholesterol (HFHC) diet for 7 weeks   (MGI Ref ID J:92448)
  • the size of the Apo-B particles are bigger in the transgenic mice fed the HFHC diet than in the wild-type control mice fed the same diet   (MGI Ref ID J:92448)
  • the size of the Apo-A-I particles are significantly smaller in the transgenic mice fed a chow diet   (MGI Ref ID J:92448)
• cardiovascular system phenotype
• increased susceptibility to atherosclerosis
  • mice have a mean lesion area 3 times that of control mice when both groups are fed a HFHC diet for 28 weeks   (MGI Ref ID J:118358)
  • significant increases in lesion area are evident by 18 weeks of age   (MGI Ref ID J:118358)
  • lesions after 28 weeks of the HFHC diet are large and focal compared to the more diffuse ribbon lesions observed in the control mice   (MGI Ref ID J:118358)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(CETP)UCTP20Pnu
Allele Name		transgene insertion UCTP20, Pharmacia & Upjohn
Allele Type		Transgenic (random, expressed)
Common Name(s)		Tg(CETP)1Pnu;
Mutation Made By		Keith Marotti,   Pharmacia-Upjohn
Strain of Origin		C57BL/6
Expressed Gene		CETP, cholesteryl ester transfer protein, plasma, simian
Expressed Gene		CETP, cholesteryl ester transfer protein, plasma, human
Promoter		CETP, cholesteryl ester transfer protein, plasma, simian
Promoter		CETP, cholesteryl ester transfer protein, plasma, human
Molecular Note		Transgenic mice overexpress the simian plasma cholesteryl ester transfer protein. Four lines were characterized with mice exhibiting a negative correlation between plasma CETP activity and total plasma cholesterol, plasma apolipoprotein A-I levels (APOA1), and plasma APOAI to APOB ratio. This line, UCTP-20, was the highest expressor of the transgene and was selected for further study. Significant transgene expression was detected by Northern blotting in the liver, brain and heart. [MGI Ref ID J:92448]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CETP), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Marotti KR; Castle CK; Murray RW; Rehberg EF; Polites HG; Melchior GW. 1992. The role of cholesteryl ester transfer protein in primate apolipoprotein A-I metabolism. Insights from studies with transgenic mice. Arterioscler Thromb 12(6):736-44. [PubMed: 1591233]  [MGI Ref ID J:92448]

Additional References

Tg(CETP)UCTP20Pnu related

Escola-Gil JC; Julve J; Marzal-Casacuberta A; Ordonez-Llanos J; Gonzalez-Sastre F; Blanco-Vaca F. 2001. ApoA-II expression in CETP transgenic mice increases VLDL production and impairs VLDL clearance. J Lipid Res 42(2):241-8. [PubMed: 11181754]  [MGI Ref ID J:128581]

Foger B; Chase M; Amar MJ; Vaisman BL; Shamburek RD; Paigen B; Fruchart-Najib J; Paiz JA; Koch CA; Hoyt RF; Brewer HB Jr; Santamarina-Fojo S. 1999. Cholesteryl ester transfer protein corrects dysfunctional high density lipoproteins and reduces aortic atherosclerosis in lecithin cholesterol acyltransferase transgenic mice. J Biol Chem 274(52):36912-20. [PubMed: 10601244]  [MGI Ref ID J:118354]

Marotti KR; Castle CK; Boyle TP; Lin AH; Murray RW; Melchior GW. 1993. Severe atherosclerosis in transgenic mice expressing simian cholesteryl ester transfer protein. Nature 364(6432):73-5. [PubMed: 8316302]  [MGI Ref ID J:118358]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	This strain is maintained by homozygous sibling matings.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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