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Strain Name:

NOD/ShiLtJ

Stock Number:

001976

Availability:

Level 1

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General Terms and Conditions

Former Name      NOD/LtJ    (Changed: 23-FEB-07 )
Strain Common Names      Non-obese Diabetic;
Genes & Alleles   Cdh23;   Cdh23ahl;   Hc;   Hc0;

Product Information

Strain Details

Type Inbred Strain
Additional information on Inbred Strains.
Mating SystemSibling x Sibling         (Female x Male)
Specieslaboratory mouse
H2 Haplotypeg7
GenerationF120 (03-JAN-08)

Appearance
albino
Related Genotype: A/A Tyrc/Tyrc

Important Note
This strain is homozygous for Cdh23ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss that is already severe by 3 months of age.

Strain Description
Diabetes in NOD/ShiLtJ mice is characterized by insulitis, a leukocytic infiltrate of the pancreatic islets. Marked decreases in pancreatic insulin content occur in females at about 12 weeks of age and several weeks later in males. Onset of diabetes is marked by moderate glycosuria and by a non-fasting plasma glucose higher than 250 mg/dl. Diabetic mice are hypoinsulinemic and hyperglucagonemic, indicating a selective destruction of pancreatic islet beta cells. Susceptibility to IDDM in NOD/ShiLtJ mice is polygenic, and environment, including housing conditions, health status, and diet, exerts a strong effect on penetrance. NOD/ShiLtJ females are more widely used than males because the onset of IDDM symptoms occurs earlier and with a higher incidence (90-100% by 30 weeks of age). NOD/ShiLtJ males develop IDDM at a frequency of between 40-60% by 30-40 weeks of age. Male mice are useful for certain applications, including pharmaceutical studies, "accelerated transfer" of IDDM, and some in vitro studies. The major component of diabetes susceptibility in NOD mice is the unique MHC haplotype (H2g7 = Kd, Aad, Abg7, Enull, Db). NOD mice also exhibit multiple aberrant immunophenotypes including defective antigen presenting cell immunoregulatory functions, defects in the regulation of theT lymphocyte repertoire, defective NK cell function, defective cytokine production from macrophages (Fan et al., 2004) and impaired wound healing. They also lack hemolytic complement, C5. NOD/ShiLtJ mice also are severely hearing-impaired. A variety of mutations causing immunodeficiencies, targeted mutations in cytokine genes, as well as transgenes affecting immune functions, have been backcrossed into the NOD/ShiLt inbred strain background.

Strain Development
NOD inbred mice originated early on in the inbreeding of the Cataract Shionogi (CTS) strain. These mice were originally outbred Jcl:ICR mice. At F6, the progenitors of the future NOD/Shi mice were inbred on the basis of an elevated fasting blood glucose level in cataract-free mice. At F13, the NOD progenitors were separated from what is now the NON/Shi strain. High fasting blood glucose levels continued to be the basis for selection of the latter strain, while the NOD progenitors at F13 and later were selected on the basis of a normal fasting blood glucose level. In 1974, at F20, a female in the "normoglycemic" line spontaneously developed overt insulin-dependent diabetes mellitus with insulitis (IDDM). Selective breeding of the progeny of this diabetic female produced the nonobese diabetic (NOD) strain. Originally restricted to distribution in Japan, NOD substrains were distributed during the early 1980s to Australia and the United States. NOD and NON strains were imported from a colony in Kyoto, Japan by Dr. M. Hattori to the Joslin Diabetes Ceneter in Boston in 1984. Breeder pairs from this importation were sent from The Joslin Diabetes Center to Dr. E Leiter at The Jackson Laboratory, and are the source of the production strains NOD/ShiLtJ and NON/ShiLtJ. The current generation of inbreeding is F83.

Gene & Allele Details

Allele Symbol Cdh23ahl
Allele Name age related hearing loss 1
Common Name(s) Cdh23753A; mdfw;
Strain of OriginC57BL/6J
Gene Symbol and Name Cdh23, cadherin 23 (otocadherin)
Chromosome 10
Gene Common Name(s) 4930542A03Rik; DFNB12; DKFZp434P2350; FLJ00233; FLJ36499; KIAA1774; KIAA1812; MGC102761; RIKEN cDNA 4930542A03 gene; USH1D; W; age related hearing loss 1; ahl; bob; bobby; bus; bustling; mdfw; modifier of deaf waddler; neuroscience mutagenesis facility, 112; neuroscience mutagenesis facility, 181; neuroscience mutagenesis facility, 252; nmf112; nmf181; nmf252; v; waltzer;
Molecular Note Genetic complementation tests have shown allelism between the mdfw (modifier of deaf waddler) locus and the ahl locus. Further analysis has identified an association between ahl and a G to A transition at nucleotide position 753 of Cdh23. This hypomorphic allele causes in frame skipping of exon 7 and reduced message stability. Twenty-seven strains classified with ahl and carrying the 753A allele include: CD1, RBF/DnJ, PL/J, AKR/J, RF/J, BALB/cBy, A/WySnJ, P/J, SENCARA/PtJ, DBA/1J, ALS/LtJ, C58/J, C57BLKS/J, 129/ReJ, C57BR/cd, SKH2/J, BUB/Bn, MA/MyJ, LP/J, 129/ScJ, NOR/LtJ, A/J, C57BL/6, NOD/LyJ, DBA/2J, ALR/LtJ, C57L/J. Strains classified with ahl that DO NOT carry this mutation include: C3H/HeSnJ, I/LnJ,YBR/Ei, MRL/MpJ. [J:86905]
 
Allele Symbol Hc0
Allele Name deficient
Common Name(s) C5-; C5-d; C5-def; C5-deficient; hco;
Gene Symbol and Name Hc, hemolytic complement
Chromosome 2
Gene Common Name(s) C5; C5a; CPAMD4; FLJ17816; FLJ17822; He; MGC142298; RGD1561905;
General Note

This is an allele characteristic of various inbred mouse strains including the following: A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ

Hc was identified as a candidate gene for Abhr2 in a microarray analysis of lung mRNA from A/J, C3H/HeJ, and (A/J x C3H/HeJ)F1 x A/J backcross animals. Hc genotype shows statistically significant correlation to allergen-induced bronchial hyperresponsive phenotype. The A/J allele contains a 2 bp deletion resulting in deficient Hc mRNA and protein production and is associated with susceptibility to allergen-induced bronchial hyperresponsiveness. (J:108211)

Molecular Note A 2 base "TA" deletion at positions 62 and 63 of an 83 base pair exon near the 5' end of the gene is found in the following mouse strains: A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ. The consequence of this deletion is the creation of a stop codon starting four bases after the deletion. A truncated product of 216 amino acids is predicted as a result although contradictory reports exist that a larger pro-C5 protein may be synthesized. Nevertheless, macrophages from mouse strains carrying this allele do not secrete complement 5. [J:23983]

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

NOD Strains
001289   NOD/ShiLt
006778   NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ
004939   NOD/ShiLtJ-Leprdb-5J/LtJ
View NOD Strains     (3 strains)

Strains carrying   Cdh23ahl allele
001137   129P1/ReJ
000690   129P3/J
002065   129T2/SvEmsJ
000691   129X1/SvJ
000646   A/J
000647   A/WySnJ
003070   ALR/LtJ
003072   ALS/LtJ
004502   B6;AKR-Lxl2/J
001026   BALB/cByJ
000653   BUB/BnJ
005494   C3.129S1(B6)-Grm1rcw/J
000664   C57BL/6J
004764   C57BL/6J-Cdh23v-8J/J
003129   C57BL/6J-Epha4rb-2J/J
004820   C57BL/6J-Kcne12J/J
004703   C57BL/6J-Nmf134/J
004811   C57BL/6J-nmf110/J
004812   C57BL/6J-nmf111/J
004747   C57BL/6J-nmf118/J
004656   C57BL/6J-nmf88/J
004391   C57BL/6J-Chr 13A/J/NaJ
004385   C57BL/6J-Chr 7A/J/NaJ
000662   C57BLKS/J
000667   C57BR/cdJ
000668   C57L/J
000669   C58/J
000657   CE/J
000670   DBA/1J
001140   DBA/1LacJ
000671   DBA/2J
007048   DBA/2J-Gpnmb+/SjJ
002106   KK/HlJ
000675   LG/J
000676   LP/J
000677   MA/MyJ
002050   NOR/LtJ
000679   P/J
002747   SENCARB/PtJ
002335   SKH2/J
003392   STOCK Crb1rd8/J
View Strains carrying   Cdh23ahl     (41 strains)

Strains carrying   Hc0 allele
000645   A/HeJ
000646   A/J
000647   A/WySnJ
000648   AKR/J
000460   B10.D2-Hc0 H2d H2-T18c/o2SnJ
000461   B10.D2-Hc0 H2d H2-T18c/oSnJ
000657   CE/J
000671   DBA/2J
007048   DBA/2J-Gpnmb+/SjJ
001800   FVB/NJ
001491   FVB/NMob
001303   NOD.CB17-Prkdcscid/J
000684   NZB/BlNJ
000682   RF/J
000688   ST/bJ
000689   SWR/J
View Strains carrying   Hc0     (16 strains)

Strains carrying other alleles of Cdh23
002756   B6.CAST-Cdh23Ahl+/Kjn
002432   B6J x B6.C-H2bm1/ByJ-Cdh23v-J/J
002552   C57BL/6J-Cdh23v-2J/J
004764   C57BL/6J-Cdh23v-8J/J
004819   C57BL/6J-Cdh23v-9J/J
005016   CByJ;B6-Cdh23v-10J/J
000275   V/LeJ
View Strains carrying other alleles of Cdh23     (7 strains)

View Strains carrying other alleles of Hc     (5 strains)

Phenotypic Data

Body Weight Information - JAX® Mice Strain NOD/ShiLtJ (001976)
(This chart reflects the typical correlation between body weight and age for mice maintained in production colonies at The Jackson Laboratory.)
Mouse Phenome Database
Festing Inbred Strain Characteristics: NOD
NEW -- JAX® Physiological Data Summary
NEW -- JAX® Physiological Data Protocol

Additional Web Information

NEW -- JAX® Physiological Data Protocol
NEW -- JAX® Physiological Data Summary
Genetic Quality Control Annual Report
JAX Notes, Spring 1999; 477. Control Strains for NOD/LtJ Mice in Diabetes Research.
JAX Notes, Spring 2003; 489. Malocclusion in the Laboratory Mouse.
JAX Notes, Spring 2003; 489. Role of NK and NKT Cells in Immunity and Disease.
JAX Notes, Spring 2007; 505. Research Using NOD/ShiLtJ Mouse Suggests Novel Therapies for Type 1 Diabetes.
JAX Notes, Summer 1992; 450. Myoepitheliomas in Inbred Laboratory Mice.
JAX Notes, Summer 2003; 490. Hydrocephalus in Laboratory Mice.
JAX Notes, Winter 1993; 452. NOD/LtJ Mice Available.
JAX Notes, Winter 1999; 476. Using NOD/LtJ Males in Diabetes Research.
JAX Notes, Winter 2006; 504. JAX® Mice: the Gold Standard Just Got Better.

Animal Health Reports

Room Number           AX8

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Lymphoid Tissue Defects (hematopoietic defects)

Diabetes and Obesity Research
Hyperglycemia
Hypoinsulinemia
Impaired Wound Healing
Islet Transplantation Studies
Type 1 Diabetes (IDDM)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)

Internal/Organ Research
Wound Healing (delayed/impaired)

Neurobiology Research
Vestibular and Hearing Defects (Age related hearing loss)

Research Tools
Immunology and Inflammation Research (NK Cell Deficiency)

Sensorineural Research
Vestibular and Hearing Defects (Age related hearing loss)

Cdh23ahl related

Neurobiology Research
Vestibular and Hearing Defects (Age related hearing loss)

Sensorineural Research
Vestibular and Hearing Defects (Age related hearing loss)

Hc0 related

Immunology and Inflammation Research
Immunodeficiency (specific complement deficiency)

Research Tools
Immunology and Inflammation Research (specific complement deficiency) (C5 complement)

References

Selected Reference(s)

Makino S; Kunimoto K; Muraoka Y; Mizushima Y; Katagiri K; Tochino Y. 1980. Breeding of a non-obese, diabetic strain of mice. Jikken Dobutsu 29(1):1-13. [PubMed: 6995140]  [J:25411]

Serreze DV; Chapman HD; Varnum DS; Gerling I; Leiter EH; Shultz LD. 1997. Initiation of autoimmune diabetes in NOD/Lt mice is MHC class I-dependent. J Immunol 158(8):3978-86. [PubMed: 9103469]  [J:39473]

Additional References

Price and Supply Information

Strain Name: NOD/ShiLtJ
Stock Number: 001976

Price Details

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Supply Details

Standard SupplyLevel 1. JAX Ready Strain® (readily available).
Supply Notes Mice are not available above eight weeks of age.
Shipped at a specific age in weeks. Mice at a precise age in days and littermates are also available.
Strains that must be genotyped are not available until five to seven weeks of age.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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