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Strain Name:

C3A.BLiA-Pde6b+.O20-Prph2Rd2/J

Stock Number:

001979

Availability:

Repository- Live

Price and Supply Information

General Terms and Conditions

Former Name      C3A.BLiA-Pde6b+.O20-RdsRd2/J    (Changed: 08-DEC-06 )
      C3A.BLiA-Pde6b+.O20-Prph2Rd2    (Changed: 15-DEC-04 )
Genes & Alleles   Pde6b;   Pde6b+;   Prph2;   Prph2Rd2;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donor Strain C57BL/LiA
Generation?+F69 (20-DEC-04)

Appearance
agouti
Related Genotype: A/A

Strain Development
This strain was developed in the laboratory of Dr. Willem J. De Grit at Erasmus Universiteit, Rotterdam, the Netherlands. The Prph2Rd2 allele was transferred from strain O20/A to C3A.BLiA-Pde6b+ via more than 20 backcross-intercross cycles (Schalken et al. 1990; S. Sanyal, personal communication). C3A.BLiA-Pde6b+ is a congenic strain having the wildtype Pde6b allele of C57BL/LiA on the C3Hf/HeA background. Since its arrival at The Jackson Laboratory, Stock No. 001979 has been maintained by sibling mating of mice homozygous for both Pde6b+ and Prph2Rd2.

Mammalian Phenotype Terms assigned by genotype

Prph2Rd2/Prph2+

        either: (involves: C3H * O20/A) or (involves: C57BL/LiA * O20/A)
  • vision/eye phenotype
  • abnormal photoreceptor outer segment morphology (MGI Ref ID J:25582)
    • in pigmented heterozygous mice outer segments resemble those in albino mice but have more disc membranes that appear more tightly packed and less vacuolated
  • nervous system phenotype
  • abnormal photoreceptor outer segment morphology (MGI Ref ID J:25582)
    • in pigmented heterozygous mice outer segments resemble those in albino mice but have more disc membranes that appear more tightly packed and less vacuolated

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Prph2Rd2/Prph2+

        either: (involves: BALB/c * O20/A) or (involves: GR/A * O20/A) or (involves: O20/A * STS/A)
  • vision/eye phenotype
  • abnormal eye development (MGI Ref ID J:25582)
    • at P14, considerable portions of the retinal surface have fewer outer segments and those present have disc structures that are irregularly oriented
  • abnormal retinal layer morphology (MGI Ref ID J:25582)
    • abnormal retinal photoreceptor morphology (MGI Ref ID J:25582)
      • at 18 months, some photoreceptor synaptic terminals have multiple synaptic ribbons
      • abnormal photoreceptor inner segment morphology (MGI Ref ID J:25582)
        • occasional inner segments with swollen endoplasmic reticuli and vacuoles
      • abnormal photoreceptor outer segment morphology (MGI Ref ID J:25582)
        • outer segments are delayed in development but eventually appear throughout the retinal surface
        • outer segments are reduced in length and morphologically abnormal appearing as round masses present from the apical end of the inner segments to the pigment epthelial villous processes
        • these round masses contain irregular whorls of disc membranes that appear to be swollen and vacuolated
        • at 18 months, outer segments become reduced and somewhat patchy
      • increased retinal cone cell number (MGI Ref ID J:25582)
        • the percentage of cones in the retina increases from 6 months on, with greater increases in the periphery than in the center of the retina
    • abnormal retinal pigment epithelium morphology (MGI Ref ID J:25582)
      • pigment epithelial cells contain larger and more numerous phagosomes
      • peak turnover of phagosomes during the light dark cycle is shifted towards the end of the light period
    • thin retinal outer nuclear layer (MGI Ref ID J:25582)
      • maximal outer nuclear layer thickness is only 50-60% of wild type
      • at 18 months, outer nuclear layer thickness is markedly decreased
      • thinning of the outer nuclear layer is first seen in the peripheral retina and later seen in the central retina
  • nervous system phenotype
  • abnormal retinal photoreceptor morphology (MGI Ref ID J:25582)
    • at 18 months, some photoreceptor synaptic terminals have multiple synaptic ribbons
    • abnormal photoreceptor inner segment morphology (MGI Ref ID J:25582)
      • occasional inner segments with swollen endoplasmic reticuli and vacuoles
    • abnormal photoreceptor outer segment morphology (MGI Ref ID J:25582)
      • outer segments are delayed in development but eventually appear throughout the retinal surface
      • outer segments are reduced in length and morphologically abnormal appearing as round masses present from the apical end of the inner segments to the pigment epthelial villous processes
      • these round masses contain irregular whorls of disc membranes that appear to be swollen and vacuolated
      • at 18 months, outer segments become reduced and somewhat patchy
    • increased retinal cone cell number (MGI Ref ID J:25582)
      • the percentage of cones in the retina increases from 6 months on, with greater increases in the periphery than in the center of the retina
  • pigmentation phenotype
  • abnormal retinal pigment epithelium morphology (MGI Ref ID J:25582)
    • pigment epithelial cells contain larger and more numerous phagosomes
    • peak turnover of phagosomes during the light dark cycle is shifted towards the end of the light period

Prph2Rd2/Prph2+

        either: C.O20-Prph2Rd2 or C3.O20-Prph2Rd2
  • vision/eye phenotype
  • abnormal Muller cell morphology (MGI Ref ID J:27850)
    • at 2 months, high numbers of Muller cells and lower numbers of fibrillar tangles and horizontal fibers are seen relative to homozygous mice
    • at 16 months, the density of Muller cells is increased compared to 2 months
  • thin retinal outer nuclear layer (MGI Ref ID J:27850)
    • at 16 months, the outer nuclear layer is reduced to 3 to 4 rows
  • nervous system phenotype
  • abnormal Muller cell morphology (MGI Ref ID J:27850)
    • at 2 months, high numbers of Muller cells and lower numbers of fibrillar tangles and horizontal fibers are seen relative to homozygous mice
    • at 16 months, the density of Muller cells is increased compared to 2 months

Prph2Rd2/Prph2Rd2

        O20/A-Prph2Rd2
  • vision/eye phenotype
  • abnormal retinal pigment epithelium morphology (MGI Ref ID J:25582)
    • phagosomes containing debris from the outer nuclear layer are absent
  • absent retinal rod cells (MGI Ref ID J:6051)
    • at 7-10 months rods are absent
  • retinal degeneration (MGI Ref ID J:6051)
    • slight degenerative changes are seen in the outer nuclear layer starting at 5 weeks
    • degeneration slowly progresses until at 7-10 months rods are absent and the photoreceptor cell nuclei are reduced to 1 row
  • retinal outer nuclear layer degeneration (MGI Ref ID J:6051)
    • slow degeneration beginning at 5 weeks and progressing until at 7-10 months rods are absent and the photoreceptor cell nuclei are reduced to 1 row
  • nervous system phenotype
  • absent retinal rod cells (MGI Ref ID J:6051)
    • at 7-10 months rods are absent
  • pigmentation phenotype
  • abnormal retinal pigment epithelium morphology (MGI Ref ID J:25582)
    • phagosomes containing debris from the outer nuclear layer are absent

Prph2Rd2/Prph2Rd2

        either: C.O20-Prph2Rd2 or C3.O20-Prph2Rd2
  • vision/eye phenotype
  • abnormal Muller cell morphology (MGI Ref ID J:27850)
    • at P11 small numbers of Muller cells and radial fibers are found in the outer plexiform layer
    • at P28 the number of Muller cells is increased, the cells tend to be unevenly distributed, and occasional fibrillar tangles are seen in the inner plexiform layer
    • at 2 months of age the density of Muller cells has increased dramatically, in the periphery these cells have fibers that penetrate the outer nuclear layer and cover it externally, and the number of fibrillar tangles is increased
    • at 9 months of age the density of Muller cells, fibrillar tangles, and horizontal fibers in the plexiform layers is further increased
  • thin retinal outer nuclear layer (MGI Ref ID J:27850)
    • at 9 months, the outer nuclear layer is reduced to 2 to 3 rows
    • at 18 months, no recognizable outer nuclear layer is seen
  • nervous system phenotype
  • abnormal Muller cell morphology (MGI Ref ID J:27850)
    • at P11 small numbers of Muller cells and radial fibers are found in the outer plexiform layer
    • at P28 the number of Muller cells is increased, the cells tend to be unevenly distributed, and occasional fibrillar tangles are seen in the inner plexiform layer
    • at 2 months of age the density of Muller cells has increased dramatically, in the periphery these cells have fibers that penetrate the outer nuclear layer and cover it externally, and the number of fibrillar tangles is increased
    • at 9 months of age the density of Muller cells, fibrillar tangles, and horizontal fibers in the plexiform layers is further increased

Gene & Allele Details

Allele Symbol Pde6b+
Allele Name wild type
Mutation Made By Frank Kooy,   University of Antwerp
Gene Symbol and Name Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Chromosome 5
Gene Common Name(s) CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10;
 
Allele Symbol Prph2Rd2
Allele Name retinal degeneration 2
Common Name(s) Prph2Rds; Rd-2; Rds; RdsRd2; rds-; retinal degeneration slow;
Strain of OriginO20/A
Gene Symbol and Name Prph2, peripherin 2
Chromosome 17
Gene Common Name(s) AOFMD; AVMD; PRPH; RDS; RP7; RSRDS; Rd-2; Rd2; Rds; TSPAN22; retinal degeneration 2; retinal degeneration, slow; retinal degeneration, slow (retinitis pigmentosa 7);
General Note The rate of retinal degeneration in mutants doubly homozygous for two retinal degeneration mutations (Pde6brd1 and RdsRd2) is intermediate between those of the two homozygotes (J:12044). The double homozygote shows an intermediate level of mRNAs for the beta subunit of cGMP-PDE and for several other phototransduction related proteins, suggesting an interaction between Pde6brd1 and RdsRd2 (J:2579).
Molecular Note The mutation is an insertion of approximately 10 kb in the gene after nucleotide 899 (numbering of the encoded mRNA), disrupting the protein coding sequence in exon 2. The inserted DNA was similar to both the TSE of mice, repeated elements found in the H2 complex, and to the mouse early transposon (ETn). Northern blot analysis demonstrated that an aberrant 12 kb transcript was produced from this allele, although at reduced levels compared to wild type. This allele is predicted to encode a truncated protein with its carboxy terminal 116 amino acids replaced by 35 amino acids from sequences in the insertion. [MGI Ref ID J:9635]

Control Information

  Control
   001912 C3A.BLiA-Pde6b+/J
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Related Strains

Strains carrying   Pde6b+ allele
002802   C3.BLiA Pde6b+-Krd/J
001912   C3A.BLiA-Pde6b+/J
003648   C3Sn.BLiA-Pde6b+/Dn
004828   FVB.129P2-Pde6b+ Tyrc-ch/AntJ
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
View Strains carrying   Pde6b+     (5 strains)

Strains carrying   Prph2Rd2 allele
001957   C3A Pde6brd1.O20/A-Prph2Rd2/J
001981   O20/A-Prph2Rd2/J
View Strains carrying   Prph2Rd2     (2 strains)

Strains carrying other alleles of Pde6b
004202   B6.C3 Pde6brd1 Hps4le/+ +-Lmx1adr-8J/J
000002   B6.C3-Pde6brd1 Hps4le/J
004297   B6.CXB1-Pde6brd10/J
001022   B6C3FeF1/J a/a
000652   BDP/J
000653   BUB/BnJ
002439   C3.129P2(B6)-B2mtm1Unc/J
005494   C3.129S1(B6)-Grm1rcw/J
000480   C3.MRL-Faslpr/J
001957   C3A Pde6brd1.O20/A-Prph2Rd2/J
005973   C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326   C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968   C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
001906   C3Ga.Cg-Catb/J
001904   C3H-Atcayji-hes/J
000659   C3H/HeJ
000784   C3H/HeJ-Faslgld/J
000509   C3H/HeJ-Lystbg-2J/J
002433   C3H/HeJ-Spnb4qv-lnd2J/J
005972   C3H/HeJBirLtJ
001824   C3H/HeJSxJ
000635   C3H/HeOuJ
000474   C3H/HeSn
001431   C3H/HeSn-ocd/J
000661   C3H/HeSnJ
002235   C3H/HeSnJ-Ctnna2cdf/J
002333   C3H/HeSnJ-gri/J
006435   C3HeB.SW-Soaa/MonJ
000658   C3HeB/FeJ
001576   C3HeB/FeJ-Atp7btx-J/J
002588   C3HeB/FeJ-Eya1bor/J
001533   C3HeB/FeJ-Mc1rE-so Gli3Xt-J/J
001886   C3HeB/FeJLe a/a-gnd/J
001908   C3HfB/BiJ
001502   C3Sn.B6-Epha4rb/J
001547   C3Sn.Cg-Cm/J
004766   C57BL/6J-Pde6brd1-2J/J
000656   CBA/J
000813   CBA/J-Atp7aMo-pew/J
000660   DA/HuSnJ
000023   FL/1ReJ
000025   FL/4ReJ
003024   FVB.129P2(B6)-Fmr1tm1Cgr/J
002539   FVB.129P2-Abcb4tm1Bor/J
002935   FVB.129S2(B6)-Ccnd1tm1Wbg/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
003078   FVB.Cg-Tg(WapIgf1)39Dlr/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
002374   FVB/N-Tg(MMTV-PyVT)634Mul/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
002384   FVB/N-Tg(UcpDta)1Kz/J
001800   FVB/NJ
003487   FVB/NJ-Tg(XGFAP-lacZ)3Mes/J
001491   FVB/NMob
000734   MOLD/RkJ
000550   MOLF/EiJ
002423   NON/ShiLtJ
000679   P/J
000680   PL/J
100299   PLSJLF1/J
000269   SB/LeJ
005651   SJL.AK-Thy1a/TseJ
000686   SJL/J
000688   ST/bJ
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002648   STOCK a/a Cln6nclf/J
000279   STOCK gr +/+ Ap3d1mh/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
004770   SW.B6-Soab/J
002023   SWR.M-Emv21 Emv22/J
000689   SWR/J
000939   SWR/J-Clcn1adr-mto/J
000692   WB/ReJ KitW/J
100410   WBB6F1/J-KitW/KitW-v/J
000693   WC/ReJ KitlSl/J
100401   WCB6F1/J KitlSl KitlSl-d
View Strains carrying other alleles of Pde6b     (77 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           A1

Research Applications

This mouse can be used to support research in many areas including:

Pde6b+ related

Mouse/Human Gene Homologs
retinitis pigmentosa, wildtype

Sensorineural Research
Retinal Degeneration (wild-type)

Prph2Rd2 related

Mouse/Human Gene Homologs
retinal degeneration, slow

Research Tools
Sensorineural Research (retinal degeneration)

Sensorineural Research
Retinal Degeneration

References

Selected Reference(s)

Schalken JJ; Janssen JJ; Sanyal S; Hawkins RK; de Grip WJ. 1990. Development and degeneration of retina in rds mutant mice: immunoassay of the rod visual pigment rhodopsin. Biochim Biophys Acta 1033(1):103-9. [PubMed: 2137350]  [MGI Ref ID J:109932]

Additional References

Price and Supply Information

Strain Name: C3A.BLiA-Pde6b+.O20-Prph2Rd2/J
Stock Number: 001979

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)Genotype(s) Provided
Individual Mouse Price $118.90Homozygous for Prph2Rd2
Pair $237.70Homozygous for Prph2Rd2 x Homozygous for Prph2Rd2

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845.
Usually shipped between four and eight weeks of age.
This strain is included in the Eye Mutant Resource within the Mouse Mutant Resource collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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