Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6J-azh/J    (Changed: 15-DEC-04 ) Type Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N12pN1

Appearance
black
Related Genotype: a/a

Description
The sperm of mice homozygous for the abnormal spermatozoon head shape mutation (azh) have a characteristic ladle shape and up to 40% lack a flagella. However, most male mice do breed and litter sizes are more than half as large as those of unaffected litter mates. There are defects in sperm-head packaging of the chromatin as well as acrosomal and flagellar development. Slight but significant reductions in measurements of sperm motility occur. In vitro, sperm from homozygous mutant mice fail to fertilize ova with intact zonae pellucidae, but are successful in fertilizing zona-free ova. Further analysis of these mice reveal defects in the microtubular manchette which surrounds the nucleus.

Control Information

	Control
	Heterozygote from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Hook1^azh/Hook1^azh

        involves: C57BL/6J * SWR/J

• reproductive system phenotype
• abnormal male germ cell morphology (MGI Ref ID J:77010)
• abnormal spermatid morphology (MGI Ref ID J:19269)
  • distorted acrosome
  • 100% of sperm nuclei have abnormal shapes
• teratozoospermia (MGI Ref ID J:13990)
  • all sperm in homozygous males are affected, having a characteristic ladle shape
  • spermatozoa are often club-shaped or crescent formed, looped in the midpiece of the flagellum and detached tails are often seen
• absent sperm flagellum (MGI Ref ID J:13990)
  • up to 40% lack a flagella
• detached sperm flagellum (MGI Ref ID J:77010)
• abnormal spermatogenesis (MGI Ref ID J:77010)
  • some focal spermatozoa formation, decreased efficiency in transition from round to elongated spermatids, and spermatozoa with abnormal head shapes are found
• abnormal spermatid morphology (MGI Ref ID J:19269)
  • distorted acrosome
  • 100% of sperm nuclei have abnormal shapes
• abnormal spermiogenesis (MGI Ref ID J:19242)
  • nuclear invaginations, nuclear evaginations, abnormal positioning of the microtubules, and ectopic manchette
• arrest of spermatogenesis (MGI Ref ID J:77010)
  • histology shows arrest of spermatogenesis at variable stages in 25% of seminiferous tubules
• teratozoospermia (MGI Ref ID J:13990)
  • all sperm in homozygous males are affected, having a characteristic ladle shape
  • spermatozoa are often club-shaped or crescent formed, looped in the midpiece of the flagellum and detached tails are often seen
• absent sperm flagellum (MGI Ref ID J:13990)
  • up to 40% lack a flagella
• detached sperm flagellum (MGI Ref ID J:77010)
• decreased litter size (MGI Ref ID J:13990)
• reduced male fertility (MGI Ref ID J:13990)
  • most males breed and litter sizes are more than half as large as those of unaffected litter mates

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Hook1^azh related

Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Hook1azh
Allele Name		abnormal spermatazoon head shape
Allele Type		Radiation induced
Common Name(s)		azh;
Strain of Origin		C57BL/6J x SWR/J
Gene Symbol and Name		Hook1, hook homolog 1 (Drosophila)
Chromosome		4
Gene Common Name(s)		A930033L17Rik; HK1; MGC10642; RIKEN cDNA A930033L17 gene; abnormal spermatazoon head shape; abnormal spermatozoon head shape; azh;
Molecular Note		The azh mutation has been identified as a deletion of exons 10 and 11. The genomic deletion was an estimated 2021 bp in size. [MGI Ref ID J:77010]

Genotyping

Genotyping Information

Genotyping Protocols

Hook1^azh, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Meistrich ML; Kasai K; Olds-Clarke P; MacGregor GR; Berkowitz AD; Tung KS. 1994. Deficiency in fertilization by morphologically abnormal sperm produced by azh mutant mice. Mol Reprod Dev 37(1):69-77. [PubMed: 8129933]  [MGI Ref ID J:16158]

Meistrich ML; Trostle-Weige PK; Russell LD. 1990. Abnormal manchette development in spermatids of azh/azh mutant mice. Am J Anat 188(1):74-86. [PubMed: 2346121]  [MGI Ref ID J:19242]

Mochida K; Tres LL; Kierszenbaum AL. 1999. Structural and biochemical features of fractionated spermatid manchettes and sperm axonemes of the azh/azh mutant mouse. Mol Reprod Dev 52(4):434-44. [PubMed: 10092124]  [MGI Ref ID J:53384]

Russell LD; Russell JA; MacGregor GR; Meistrich ML. 1991. Linkage of manchette microtubules to the nuclear envelope and observations of the role of the manchette in nuclear shaping during spermiogenesis in rodents. Am J Anat 192(2):97-120. [PubMed: 1759685]  [MGI Ref ID J:19269]

Additional References

Mendoza-Lujambio I; Burfeind P; Dixkens C; Meinhardt A; Hoyer-Fender S; Engel W; Neesen J. 2002. The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse. Hum Mol Genet 11(14):1647-58. [PubMed: 12075009]  [MGI Ref ID J:77010]

Hook1^azh related

Akutsu H; Tres LL; Tateno H; Yanagimachi R; Kierszenbaum AL. 2001. Offspring from normal mouse oocytes injected with sperm heads from the azh/azh mouse display more severe sperm tail abnormalities than the original mutant. Biol Reprod 64(1):249-56. [PubMed: 11133681]  [MGI Ref ID J:68453]

Hugenholtz AP. 1984. Abnormal spermatazoon head shape Mouse News Lett 71:35.  [MGI Ref ID J:13990]

Mendoza-Lujambio I; Burfeind P; Dixkens C; Meinhardt A; Hoyer-Fender S; Engel W; Neesen J. 2002. The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse. Hum Mol Genet 11(14):1647-58. [PubMed: 12075009]  [MGI Ref ID J:77010]

Moreno RD; Palomino J; Schatten G. 2006. Assembly of spermatid acrosome depends on microtubule organization during mammalian spermiogenesis. Dev Biol 293(1):218-27. [PubMed: 16540102]  [MGI Ref ID J:108463]

Schumacher JM; Artzt K; Braun RE. 1998. Spermatid perinuclear ribonucleic acid-binding protein binds microtubules in vitro and associates with abnormal manchettes in vivo in mice. Biol Reprod 59(1):69-76. [PubMed: 9674995]  [MGI Ref ID J:89468]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Heterozygote from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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