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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.SJL-Ptprca Pep3b/BoyJ (Changed: 02-MAR-06 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Sibling x Sibling (Female x Male) Species laboratory mouse Background Strain C57BL/6 Donor Strain SJL/J Generation N22F62 (03-JAN-08) 
Appearance
black
Related Genotype: a/aDescription
This is a congenic strain used in transplant studies because it carries the differential B cell antigen originally designated Ly5.1 and CD45.1 The current use of the Ptprc designation for Cd45 and Ly5 was based on work in humans following the report of Charbonneau and colleagues who first showed that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) was homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth. The b allele is normally present in the BALB and C57BL inbred strains.Development
The Ptprca allele and closely linked Pepcb allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprca by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
JAX® NOTES, Summer 1994; 458. Ly5 Gene Nomenclature, C57BL/6J and SJL/J - A History of Change.
Phenotype
Phenotype Information
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Genetics Research (Tissue/Cell Markers: B cell specific marker)
Genes & Alleles
Gene & Allele Information
| Gene Symbol and Name | Pepc, peptidase C | ||
|---|---|---|---|
| Chromosome | 1 | ||
| Gene Common Name(s) | Dip-1; Pep-3; Pep3; dipeptidase 1; peptidase 3; | ||
| Gene Symbol and Name | Ptprc, protein tyrosine phosphatase, receptor type, C | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Janowska-Wieczorek A; Majka M; Kijowski J; Baj-Krzyworzeka M; Reca R; Turner AR; Ratajczak J; Emerson SG; Kowalska MA; Ratajczak MZ. 2001. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood 98(10):3143-9. [PubMed: 11698303] [MGI Ref ID J:109863]
Schluns KS; Williams K; Ma A; Zheng XX; Lefrancois L. 2002. Cutting edge: requirement for IL-15 in the generation of primary and memory antigen-specific CD8 T cells. J Immunol 168(10):4827-31. [PubMed: 11994430] [MGI Ref ID J:109854]
Yang G; Hisha H; Cui Y; Fan T; Jin T; Li Q; Lian Z; Hosaka N; Li Y; Ikehara S. 2002. A new assay method for late CFU-S formation and long-term reconstituting activity using a small number of pluripotent hemopoietic stem cells. Stem Cells 20(3):241-8. [PubMed: 12004082] [MGI Ref ID J:109853]
Additional References
Charbonneau H; Tonks NK; Walsh KA; Fischer EH. 1988. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc Natl Acad Sci U S A 85(19):7182-6. [PubMed: 2845400] [MGI Ref ID J:22340]
Held W; Clevers H; Grosschedl R. 2003. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33(5):1393-8. [PubMed: 12731066] [MGI Ref ID J:85394]
Morse HC 3rd. 1992. Genetic nomenclature for loci controlling surface antigens of mouse hemopoietic cells [published erratum appears in J Immunol 1993 Mar 15;150(6):2563] J Immunol 149(10):3129-34. [PubMed: 1431091] [MGI Ref ID J:3170]
Morse HC 3rd; Shen FW; Hammerling U. 1987. Genetic nomenclature for loci controlling mouse lymphocyte antigens. Immunogenetics 25(2):71-8. [PubMed: 3817907] [MGI Ref ID J:8603]
Shen FW; Saga Y; Litman G; Freeman G; Tung JS; Cantor H; Boyse EA. 1985. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A 82(21):7360-3. [PubMed: 3864163] [MGI Ref ID J:8080]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX4
Colony Maintenance
Mating System Sibling x Sibling (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided 3 weeks $43.20 Female Homozygous for Ptprca , Homozygous for Pepcb $38.00 Male Homozygous for Ptprca , Homozygous for Pepcb 4 weeks $43.20 Female Homozygous for Ptprca , Homozygous for Pepcb $38.00 Male Homozygous for Ptprca , Homozygous for Pepcb 5 weeks $43.20 Female Homozygous for Ptprca , Homozygous for Pepcb $38.00 Male Homozygous for Ptprca , Homozygous for Pepcb 6 weeks $46.90 Female Homozygous for Ptprca , Homozygous for Pepcb $41.90 Male Homozygous for Ptprca , Homozygous for Pepcb 7 weeks $46.90 Female Homozygous for Ptprca , Homozygous for Pepcb $41.90 Male Homozygous for Ptprca , Homozygous for Pepcb 8 weeks $46.90 Female Homozygous for Ptprca , Homozygous for Pepcb $41.90 Male Homozygous for Ptprca , Homozygous for Pepcb
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided 3 weeks $56.20 Female Homozygous for Ptprca , Homozygous for Pepcb $49.40 Male Homozygous for Ptprca , Homozygous for Pepcb 4 weeks $56.20 Female Homozygous for Ptprca , Homozygous for Pepcb $49.40 Male Homozygous for Ptprca , Homozygous for Pepcb 5 weeks $56.20 Female Homozygous for Ptprca , Homozygous for Pepcb $49.40 Male Homozygous for Ptprca , Homozygous for Pepcb 6 weeks $61.00 Female Homozygous for Ptprca , Homozygous for Pepcb $54.50 Male Homozygous for Ptprca , Homozygous for Pepcb 7 weeks $61.00 Female Homozygous for Ptprca , Homozygous for Pepcb $54.50 Male Homozygous for Ptprca , Homozygous for Pepcb 8 weeks $61.00 Female Homozygous for Ptprca , Homozygous for Pepcb $54.50 Male Homozygous for Ptprca , Homozygous for Pepcb
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Level 2. Up to 100 mice. Larger quantities or custom orders arranged upon request. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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