Strain Name:

B6.SJL-Ptprca Pepcb/BoyJ

Stock Number:

002014

Availability:

Level 2

This is a congenic strain used in transplant studies because it carries the differential B cell antigen originally designated Ly5.1 and CD45.1. The b allele of Ptprc is normally present in the BALB and C57BL inbred strains.

Description

Strain Information

Former Names B6.SJL-Ptprca Pep3b/BoyJ    (Changed: 02-MAR-06 )
Type Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemSibling x Sibling         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain SJL/J
GenerationN22F62 (03-JAN-08)

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Appearance
black
Related Genotype: a/a

Description
This is a congenic strain used in transplant studies because it carries the differential B cell antigen originally designated Ly5.1 and CD45.1 The current use of the Ptprc designation for Cd45 and Ly5 was based on work in humans following the report of Charbonneau and colleagues who first showed that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) was homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth. The b allele is normally present in the BALB and C57BL inbred strains.

Development
The Ptprca allele and closely linked Pepcb allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprca by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pepc
006259   B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J
000824   LT.B6-Pepca/J
View Strains carrying other alleles of Pepc     (2 strains)

View Strains carrying other alleles of Ptprc     (11 strains)

Additional Web Information

Genetic Quality Control Annual Report
JAX® NOTES, Summer 1994; 458. Ly5 Gene Nomenclature, C57BL/6J and SJL/J - A History of Change.

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers: B cell specific marker

Genes & Alleles

Gene & Allele Information

 
Gene Symbol and Name Pepc, peptidase C
Chromosome 1
Gene Common Name(s) Dip-1; Pep-3; Pep3; dipeptidase 1; peptidase 3;
 
Gene Symbol and Name Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome 1
Gene Common Name(s) B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;

Genotyping

Genotyping Information

The Jackson Laboratory has not developed a genotyping assay for the B6.SJL-Ptprca Pepcb/BoyJ mouse strain. The following reference describes an assay: Zebedee SL et al. 1991. Eur J Immunogenet 1991 Jun;18(3):155-63. PMID: 1834169. Alternatively, antibodies for Ptprca (CD45.1, Ly5.1) can be used with FACS analysis.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Janowska-Wieczorek A; Majka M; Kijowski J; Baj-Krzyworzeka M; Reca R; Turner AR; Ratajczak J; Emerson SG; Kowalska MA; Ratajczak MZ. 2001. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood 98(10):3143-9. [PubMed: 11698303]  [MGI Ref ID J:109863]

Schluns KS; Williams K; Ma A; Zheng XX; Lefrancois L. 2002. Cutting edge: requirement for IL-15 in the generation of primary and memory antigen-specific CD8 T cells. J Immunol 168(10):4827-31. [PubMed: 11994430]  [MGI Ref ID J:109854]

Yang G; Hisha H; Cui Y; Fan T; Jin T; Li Q; Lian Z; Hosaka N; Li Y; Ikehara S. 2002. A new assay method for late CFU-S formation and long-term reconstituting activity using a small number of pluripotent hemopoietic stem cells. Stem Cells 20(3):241-8. [PubMed: 12004082]  [MGI Ref ID J:109853]

Additional References

Charbonneau H; Tonks NK; Walsh KA; Fischer EH. 1988. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc Natl Acad Sci U S A 85(19):7182-6. [PubMed: 2845400]  [MGI Ref ID J:22340]

Held W; Clevers H; Grosschedl R. 2003. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33(5):1393-8. [PubMed: 12731066]  [MGI Ref ID J:85394]

Morse HC 3rd. 1992. Genetic nomenclature for loci controlling surface antigens of mouse hemopoietic cells [published erratum appears in J Immunol 1993 Mar 15;150(6):2563] J Immunol 149(10):3129-34. [PubMed: 1431091]  [MGI Ref ID J:3170]

Morse HC 3rd; Shen FW; Hammerling U. 1987. Genetic nomenclature for loci controlling mouse lymphocyte antigens. Immunogenetics 25(2):71-8. [PubMed: 3817907]  [MGI Ref ID J:8603]

Shen FW; Saga Y; Litman G; Freeman G; Tung JS; Cantor H; Boyse EA. 1985. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A 82(21):7360-3. [PubMed: 3864163]  [MGI Ref ID J:8080]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX4
Room Number           RB03

Colony Maintenance

Mating SystemSibling x Sibling         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
3 weeks $43.20FemaleHomozygous for Ptprca , Homozygous for Pepcb
$38.00MaleHomozygous for Ptprca , Homozygous for Pepcb
4 weeks $43.20FemaleHomozygous for Ptprca , Homozygous for Pepcb
$38.00MaleHomozygous for Ptprca , Homozygous for Pepcb
5 weeks $45.35FemaleHomozygous for Ptprca , Homozygous for Pepcb
$38.00MaleHomozygous for Ptprca , Homozygous for Pepcb
6 weeks $49.25FemaleHomozygous for Ptprca , Homozygous for Pepcb
$44.00MaleHomozygous for Ptprca , Homozygous for Pepcb
7 weeks $49.25FemaleHomozygous for Ptprca , Homozygous for Pepcb
$44.00MaleHomozygous for Ptprca , Homozygous for Pepcb
8 weeks $49.25FemaleHomozygous for Ptprca , Homozygous for Pepcb
$44.00MaleHomozygous for Ptprca , Homozygous for Pepcb

Additional Supply Details

Supply Notes
  • Volume discounts are available for this strain. View our Volume Pricing Program for information on quantities and discounts.

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
3 weeks $56.20FemaleHomozygous for Ptprca , Homozygous for Pepcb
$49.40MaleHomozygous for Ptprca , Homozygous for Pepcb
4 weeks $56.20FemaleHomozygous for Ptprca , Homozygous for Pepcb
$49.40MaleHomozygous for Ptprca , Homozygous for Pepcb
5 weeks $59.00FemaleHomozygous for Ptprca , Homozygous for Pepcb
$49.40MaleHomozygous for Ptprca , Homozygous for Pepcb
6 weeks $64.10FemaleHomozygous for Ptprca , Homozygous for Pepcb
$57.20MaleHomozygous for Ptprca , Homozygous for Pepcb
7 weeks $64.10FemaleHomozygous for Ptprca , Homozygous for Pepcb
$57.20MaleHomozygous for Ptprca , Homozygous for Pepcb
8 weeks $64.10FemaleHomozygous for Ptprca , Homozygous for Pepcb
$57.20MaleHomozygous for Ptprca , Homozygous for Pepcb

Additional Supply Details

Supply Details

Standard SupplyLevel 2. Up to 100 mice. Larger quantities or custom orders arranged upon request.
Supply Notes
  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
  • Strains that must be genotyped are not available until five to seven weeks of age.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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