Description

Strain Information

Former Names B6.SJL-Ptprca Pep3b/BoyJ    (Changed: 02-MAR-06 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Sibling x Sibling         (Female x Male)   01-MAR-06 Species laboratory mouse Background Strain C57BL/6 Donor Strain SJL/J Generation N22F62 (03-JAN-08)

View larger image

Appearance
black
Related Genotype: a/a

Description
This is a congenic strain used in transplant studies because it carries the differential B cell antigen originally designated Ly5.1 and CD45.1 The current use of the Ptprc designation for Cd45 and Ly5 was based on work in humans following the report of Charbonneau and colleagues who first showed that a protein-tyrosine phosphatase (human placental protein-tyrosine phosphatase 1B) was homologous to the CD45 protein. Ptprc is one of a family of protein-tyrosine phosphatase genes involved in the regulation of cell growth. The b allele is normally present in the BALB and C57BL inbred strains.

Development
The Ptprc^a allele and closely linked Pepc^b allele were serially backcrossed from SJL/J onto C57BL/6 with selection at each generation for Ptprc^a by serotyping (Shen et al., 1985). In 1990 this strain, then housed at Sloan Kettering, was imported into The Jackson Laboratory from Edward Boyse at generation N22. Since its arrival, the strain has been maintained by sibling inbreeding. In 2005 it reached generation N22F55.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pepc

006259	B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J
000824	LT.B6-Pepca/J

View Strains carrying other alleles of Pepc     (2 strains)

Strains carrying other alleles of Ptprc

007172	129S6.129X1(B6)-Ptprctm1Weis/J
002725	B6.129-Ptprctm1Holm/J
008451	B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
007594	B6.129P2-Ptrpca Ightm1Mnz/J
007171	B6.129X1-Ptprctm1Weis/J
006259	B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J
007687	BKa.Cg-Sox17tm1Sjm Ptprcb Thy1a/J
007686	BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J
007173	C.129X1(B6)-Ptprctm1Weis/J
006584	CBy.SJL(B6)-Ptprca/J
005616	NOD.C-(Ptprc-D1Mit262)/WehiJ

View Strains carrying other alleles of Ptprc     (11 strains)

Additional Web Information

Genetic Quality Control Annual Report
JAX^® NOTES, Summer 1994; 458. Ly5 Gene Nomenclature, C57BL/6J and SJL/J - A History of Change.

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers: B cell specific marker

Genes & Alleles

Gene & Allele Information

Gene Symbol and Name		Pepc, peptidase C
Chromosome		1
Gene Common Name(s)		Dip-1; Pep-3; Pep3; dipeptidase 1; peptidase 3;
Gene Symbol and Name		Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome		1
Gene Common Name(s)		B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;

Genotyping

Genotyping Information

The Jackson Laboratory has not developed a genotyping assay for the B6.SJL-Ptprc^a Pepc^b/BoyJ mouse strain. The following reference describes an assay: Zebedee SL et al. 1991. Eur J Immunogenet 1991 Jun;18(3):155-63. PMID: 1834169. Alternatively, antibodies for Ptprca (CD45.1, Ly5.1) can be used with FACS analysis.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Janowska-Wieczorek A; Majka M; Kijowski J; Baj-Krzyworzeka M; Reca R; Turner AR; Ratajczak J; Emerson SG; Kowalska MA; Ratajczak MZ. 2001. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood 98(10):3143-9. [PubMed: 11698303]  [MGI Ref ID J:109863]

Schluns KS; Williams K; Ma A; Zheng XX; Lefrancois L. 2002. Cutting edge: requirement for IL-15 in the generation of primary and memory antigen-specific CD8 T cells. J Immunol 168(10):4827-31. [PubMed: 11994430]  [MGI Ref ID J:109854]

Yang G; Hisha H; Cui Y; Fan T; Jin T; Li Q; Lian Z; Hosaka N; Li Y; Ikehara S. 2002. A new assay method for late CFU-S formation and long-term reconstituting activity using a small number of pluripotent hemopoietic stem cells. Stem Cells 20(3):241-8. [PubMed: 12004082]  [MGI Ref ID J:109853]

Additional References

Charbonneau H; Tonks NK; Walsh KA; Fischer EH. 1988. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc Natl Acad Sci U S A 85(19):7182-6. [PubMed: 2845400]  [MGI Ref ID J:22340]

Held W; Clevers H; Grosschedl R. 2003. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33(5):1393-8. [PubMed: 12731066]  [MGI Ref ID J:85394]

Morse HC 3rd. 1992. Genetic nomenclature for loci controlling surface antigens of mouse hemopoietic cells [published erratum appears in J Immunol 1993 Mar 15;150(6):2563] J Immunol 149(10):3129-34. [PubMed: 1431091]  [MGI Ref ID J:3170]

Morse HC 3rd; Shen FW; Hammerling U. 1987. Genetic nomenclature for loci controlling mouse lymphocyte antigens. Immunogenetics 25(2):71-8. [PubMed: 3817907]  [MGI Ref ID J:8603]

Shen FW; Saga Y; Litman G; Freeman G; Tung JS; Cantor H; Boyse EA. 1985. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A 82(21):7360-3. [PubMed: 3864163]  [MGI Ref ID J:8080]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX4
Room Number           RB03

Colony Maintenance

Mating System	Sibling x Sibling         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Level 2. Up to 100 mice. Larger quantities or custom orders arranged upon request.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.