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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Major Histocompatibility Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Sibling x Sibling (Female x Male) Species laboratory mouse Background Strain C57BL/10SnSg Donor Strain DBA/1 Generation N11F?+46 (03-JAN-08) Appearance
black
Related Genotype: a/aImportant Note
This strain is homozygous for a naturally occurring missense mutation in Tyk2 resulting in a loss of protein function. The mutation is associated with lymphocyte hyporesponsiveness to interleukin 12. Disruption of the IL12 signaling pathway results in an increased susceptibility to intracellular pathogens such as Toxoplasma gondii and a resistance to autoimmune disease such as collagen induced arthritis.
Control Information
| Control | ||
|---|---|---|
| 000666 C57BL/10SnJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying H2q allele
000945 B6.SW/1CyJ 002032 NOD.SW-H2q/J View Strains carrying H2q (2 strains)
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:H2q related
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Genes & Alleles
Gene & Allele Information
| Allele Symbol | H2q | ||
|---|---|---|---|
| Allele Name | q variant | ||
| Allele Type | Not Applicable | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note |
The q variant has been observed in the following strains: DBA/1, DBA/1J, BUB/BnJ, STOLI/Lw, SWR/J Genbank ID for this allele: M14827 | ||
| Allele Symbol | Tyk2E775K | ||
| Allele Name | E775K | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | B10.D1-H2q/SgJ | ||
| Gene Symbol and Name | Tyk2, tyrosine kinase 2 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | JTK1; | ||
| Molecular Note | This allele corresponds to a substitution of G to A at nucleotide position 2538, resulting in an amino acid substitution that replaces glutamic acid with lysine at position 775. The missense mutation occurs at highly conserved APE motif of the Jak homology 2 (JH2) domain. Although mRNA transcript is detectable in spleen, anti-Tyk2 specific antibody cannot detect protein either in the activated or inactivated form. [MGI Ref ID J:85848] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Ortmann R; Smeltz R; Yap G; Sher A; Shevach EM. 2001. A heritable defect in IL-12 signaling in B10.Q/J mice. I. In vitro analysis. J Immunol 166(9):5712-9. [PubMed: 11313413] [MGI Ref ID J:69051]
Shaw MH; Boyartchuk V; Wong S; Karaghiosoff M; Ragimbeau J; Pellegrini S; Muller M; Dietrich WF; Yap GS. 2003. A natural mutation in the Tyk2 pseudokinase domain underlies altered susceptibility of B10.Q/J mice to infection and autoimmunity. Proc Natl Acad Sci U S A 100(20):11594-9. [PubMed: 14500783] [MGI Ref ID J:85848]
Yap GS; Ortmann R; Shevach E; Sher A. 2001. A heritable defect in IL-12 signaling in B10.Q/J mice. II. Effect on acute resistance to Toxoplasma gondii and rescue by IL-18 treatment. J Immunol 166(9):5720-5. [PubMed: 11313414] [MGI Ref ID J:120304]
H2q relatedTyk2E775K relatedChen K; Wei Y; Sharp GC; Braley-Mullen H. 2001. Induction of experimental autoimmune thyroiditis in IL-12-/- mice. J Immunol 167(3):1720-7. [PubMed: 11466396] [MGI Ref ID J:120397]
Fischer Lindahl K. 1997. On naming H2 haplotypes: functional significance of MHC class Ib alleles. Immunogenetics 46(1):53-62. [PubMed: 9148789] [MGI Ref ID J:41130]
Hultqvist M; Olofsson P; Holmberg J; Backstrom BT; Tordsson J; Holmdahl R. 2004. Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene. Proc Natl Acad Sci U S A 101(34):12646-51. [PubMed: 15310853] [MGI Ref ID J:92437]
Jasinski JM; Yu L; Nakayama M; Li MM; Lipes MA; Eisenbarth GS; Liu E. 2006. Transgenic insulin (B:9-23) T-cell receptor mice develop autoimmune diabetes dependent upon RAG genotype, H-2g7 homozygosity, and insulin 2 gene knockout. Diabetes 55(7):1978-84. [PubMed: 16804066] [MGI Ref ID J:111874]
Klein J; Figueroa F; David CS. 1983. H-2 haplotypes, genes and antigens: second listing. II. The H-2 complex. Immunogenetics 17(6):553-96. [PubMed: 6407984] [MGI Ref ID J:7097]
Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin. [MGI Ref ID J:110093]
Lin H; Rathmell JC; Gray GS; Thompson CB; Leiden JM; Alegre ML. 1998. Cytotoxic T lymphocyte antigen 4 (CTLA4) blockade accelerates the acute rejection of cardiac allografts in CD28-deficient mice: CTLA4 can function independently of CD28. J Exp Med 188(1):199-204. [PubMed: 9653096] [MGI Ref ID J:115143]
Murphy DB. 1986. Overview: the murine MHC. In: Handbook of Experimental Immunology. Vol. 3, Genetic and Molecular Immunology. Blackwell Scientific Publ., Oxford. [MGI Ref ID J:30731]
Nicholson SM; Jokinen DM; Canto MC; Kim BS; Melvold RW. 1995. Genetic analysis of susceptibility to Theiler's murine encephalomyelitis virus-induced demyelinating disease in the SWR strain. J Neuroimmunol 59(1-2):19-28. [PubMed: 7797616] [MGI Ref ID J:27344]
Ortman RA; Holderbaum D; Qu XM; Banerjee S; Haqqi TM. 1994. BUB/BnJ (H-2q) is a TCR deletion mutant mouse strain (TCR V beta a, KJ16-) that is susceptible to type II collagen-induced arthritis. J Immunol 152(8):4175-82. [PubMed: 8144978] [MGI Ref ID J:17601]
Reed-Loisel LM; Sullivan BA; Laur O; Jensen PE. 2005. An MHC class Ib-restricted TCR that cross-reacts with an MHC class Ia molecule. J Immunol 174(12):7746-52. [PubMed: 15944277] [MGI Ref ID J:109978]
Verschelde C; Michonneau D; Trescol-Biemont MC; Berberich I; Schimpl A; Bonnefoy-Berard N. 2006. Overexpression of the antiapoptotic protein A1 promotes the survival of double positive thymocytes awaiting positive selection. Cell Death Differ 13(7):1213-21. [PubMed: 16294210] [MGI Ref ID J:126130]
Yap GS; Ortmann R; Shevach E; Sher A. 2001. A heritable defect in IL-12 signaling in B10.Q/J mice. II. Effect on acute resistance to Toxoplasma gondii and rescue by IL-18 treatment. J Immunol 166(9):5720-5. [PubMed: 11313414] [MGI Ref ID J:120304]
Ortmann R; Smeltz R; Yap G; Sher A; Shevach EM. 2001. A heritable defect in IL-12 signaling in B10.Q/J mice. I. In vitro analysis. J Immunol 166(9):5712-9. [PubMed: 11313413] [MGI Ref ID J:69051]
Shaw MH; Boyartchuk V; Wong S; Karaghiosoff M; Ragimbeau J; Pellegrini S; Muller M; Dietrich WF; Yap GS. 2003. A natural mutation in the Tyk2 pseudokinase domain underlies altered susceptibility of B10.Q/J mice to infection and autoimmunity. Proc Natl Acad Sci U S A 100(20):11594-9. [PubMed: 14500783] [MGI Ref ID J:85848]
Yap GS; Ortmann R; Shevach E; Sher A. 2001. A heritable defect in IL-12 signaling in B10.Q/J mice. II. Effect on acute resistance to Toxoplasma gondii and rescue by IL-18 treatment. J Immunol 166(9):5720-5. [PubMed: 11313414] [MGI Ref ID J:120304]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX29
Room Number MP19
Colony Maintenance
Mating System Sibling x Sibling (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided 3-5 weeks $35.20 Female or Male Homozygous for H2q 6 weeks $37.10 Female or Male Homozygous for H2q 7 weeks $39.00 Female or Male Homozygous for H2q 8 weeks $40.90 Female or Male Homozygous for H2q 9 weeks $42.80 Female or Male Homozygous for H2q 10 weeks $44.70 Female or Male Homozygous for H2q
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided 3-5 weeks $45.80 Female or Male Homozygous for H2q 6 weeks $48.30 Female or Male Homozygous for H2q 7 weeks $50.80 Female or Male Homozygous for H2q 8 weeks $53.30 Female or Male Homozygous for H2q 9 weeks $55.80 Female or Male Homozygous for H2q 10 weeks $58.30 Female or Male Homozygous for H2q
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Level 3. Up to 50 mice. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
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| Important Note | This strain is homozygous for a naturally occurring missense mutation in Tyk2 resulting in a loss of protein function. The mutation is associated with lymphocyte hyporesponsiveness to interleukin 12. Disruption of the IL12 signaling pathway results in an increased susceptibility to intracellular pathogens such as Toxoplasma gondii and a resistance to autoimmune disease such as collagen induced arthritis. |
Control Information
| Control | ||
|---|---|---|
| 000666 C57BL/10SnJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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