Description

Strain Information

Former Names 129S8/SvEv-Gpi1c Hprtb-m2/J    (Changed: 23-NOV-05 ) 129S8/SvEv@J-Gpi1c Hprtb-m2    (Changed: 15-DEC-04 ) Type Insertion; Additional information on Mice with Chromosomal Aberrations. Type Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation ?+F5p

Development
The Hprt1^b-m2 mutation was induced in ES cells by retroviral insertion.

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Gpi1

005730	129S6.CB(B6)-Del(1)1Brk Gpi1b/Gpi1c/BrkMdfJ
000600	B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
000377	B6.C-H22c Gpi1a/(HW38)ByJ
000123	B6.C-H24c Gpi1a/(HW54)ByJ
001622	B6.CAST-Gpi1a.Cg-Hbath-J
000797	B6.CAST-Gpi1a/EiJ
006561	B6.CBy(Cg)-Gpi1a-m1J/BrkJ
006562	B6.CBy(Cg)-Gusbmps Gpi1a-m1J/BrkJ
002074	B6.Cg-Gpi1a Hbbd H1b/DehJ
001317	B6.Cg-Igha Thy1a Gpi1a/J
000909	B6CBy-Gpi1a/Gpi1b/J or Gpi1a-m1/Gpi1b/J
002800	C3.Cg-Gpi1a/J
006560	CBy(Cg)-Gpi1a-m1J/BrkJ
003585	NOD.B6-(Gpi1-D7Mit346)/LtJ

View Strains carrying other alleles of Gpi1     (14 strains)

Strains carrying other alleles of Hprt1

004302	129S1-Hprt1tm1(cre)Mnn/J
002171	B6.129P2-Hprt1b-m3/J
003138	B6;129-Hprt1tm1Detl/J
000807	RBJ/DnJ

View Strains carrying other alleles of Hprt1     (4 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Hprt1^b-m2/Y

        involves: 129S/SvEv

• behavior/neurological phenotype
• *normal* behavior/neurological phenotype (MGI Ref ID J:15485)
  • mice exhibit normal behavior

Hprt1^b-m2/Y

        involves: 129 * 129S/SvEv

• nervous system phenotype
• decreased dopamine level (MGI Ref ID J:115724)
  • striatal concentrations of dopamine are lower than in wild-type mice
• behavior/neurological phenotype
• *normal* behavior/neurological phenotype (MGI Ref ID J:115724)
  • mice exhibit overall similar behavior as observed in wild-type mice in open field, bridge, passive avoidance and apomorphine-induced activity tests
• abnormal locomotor activation (MGI Ref ID J:115724)
  • male mice spend less time in the center of an open field than Hprt1^b-m1 male mice
• abnormal motor coordination/ balance (MGI Ref ID J:115724)
  • male mice make more slips when climbing than escape platform in a swim test compared to Hprt1^b-m1 male mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Mouse/Human Gene Homologs
Lesch-Nyhan syndrome

Genes & Alleles

Gene & Allele Information

Allele Symbol		Gpi1c
Allele Name		c variant
Allele Type		Not Applicable
Strain of Origin		wild
Gene Symbol and Name		Gpi1, glucose phosphate isomerase 1
Chromosome		7
Gene Common Name(s)		AMF; GNPI; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; MF; NK; NK/GPI; NLK; Org; PGI; PHI; SA-36; autocrine motility factor; glucose phosphate isomerase 1, regulatory; glucose phosphate isomerase 1, structural; glucose phosphate isomerase 1, temporal; maturation factor; neuroleukin;
Molecular Note		This allele determines a fast migrating variant of the encoded enzyme and occurs in the wild.
Allele Symbol		Hprt1b-m2
Allele Name		hypoxanthine guanine phosphoribosyl transferase B, mutation 2
Allele Type		Transgenic (random, gene disruption)
Common Name(s)		Hprt-bm2; hprtmb2;
Strain of Origin		129S/SvEv-Gpi1
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Hprt1, hypoxanthine guanine phosphoribosyl transferase 1
Chromosome		X
Gene Common Name(s)		C81579; HGPRT; HPGRT; HPRT; Hgprtase; MGC112554; expressed sequence C81579;
Molecular Note		A retroviral sequence was inserted into intron 5. [MGI Ref ID J:3354]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Williamson DJ; Hooper ML; Melton DW. 1992. Mouse models of hypoxanthine phosphoribosyltransferase deficiency. J Inherit Metab Dis 15(4):665-73. [PubMed: 1528024]  [MGI Ref ID J:3354]

Additional References

Gpi1^c related

Padua RA; Bulfield G; Peters J. 1978. Biochemical genetics of a new glucosephosphate isomerase allele (Gpi-1c) from wild mice. Biochem Genet 16(1-2):127-43. [PubMed: 25645]  [MGI Ref ID J:5973]

Hprt1^b-m2 related

Finger S; Heavens RP; Sirinathsinghji DJ; Kuehn MR; Dunnett SB. 1988. Behavioral and neurochemical evaluation of a transgenic mouse model of Lesch-Nyhan syndrome. J Neurol Sci 86(2-3):203-13. [PubMed: 3221240]  [MGI Ref ID J:115724]

Kuehn MR; Bradley A; Robertson EJ; Evans MJ. 1987. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326(6110):295-8. [PubMed: 3029599]  [MGI Ref ID J:15485]

Zheng B; Mills AA; Bradley A. 1999. A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice. Nucleic Acids Res 27(11):2354-60. [PubMed: 10325425]  [MGI Ref ID J:115976]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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