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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names C57BL/6-Tg(g2b/mumem)3Storb/J (Changed: 18-JUN-08 ) Type Coisogenic; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N3
Related Strains
Strains carrying other alleles of Igh-6
View Strains carrying other alleles of Igh-6 (12 strains)
Phenotype
Phenotype Information
assigned by genotype
Tg(Igh-3/Igh-6VD93)1241Ust/?
C57BL/6-Tg(Igh-3/Igh-6VD93)1241Ust
- immune system phenotype
- abnormal B cell morphology (MGI Ref ID J:135209)
- splenic B cells co-express gamma 2b (Igh-3) and mu (Igh-6) on all Igh-3+ cells
- decreased pre-B cell number (MGI Ref ID J:135209)
- less than 1% of splenic lymphocytes are either mu or kappa positive in 2 week old mice
- by 4 weeks of age, mice exhibit approximately 3 fold fewer mu or kappa positive splenic B cells
- hematopoietic system phenotype
- abnormal B cell morphology (MGI Ref ID J:135209)
- splenic B cells co-express gamma 2b (Igh-3) and mu (Igh-6) on all Igh-3+ cells
- decreased pre-B cell number (MGI Ref ID J:135209)
- less than 1% of splenic lymphocytes are either mu or kappa positive in 2 week old mice
- by 4 weeks of age, mice exhibit approximately 3 fold fewer mu or kappa positive splenic B cells
This mouse can be used to support research in many areas including:
Igh-6 relatedImmunology and Inflammation Research
Immunology and Inflammation Research
Immunodeficiency (B cell deficiency)
Research Tools
Cancer Research (B cell deficiency)
Immunology and Inflammation Research (B cell deficiency)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Igh-3/Igh-6VD93)1241Ust | ||
|---|---|---|---|
| Allele Name | transgene insertion 1241 Ursula Storb | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(Igh)Bri128; Tg(g2b/mumem)3Storb; g2b-mumem; | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Igh-6, immunoglobulin heavy chain 6 (heavy chain of IgM), mouse, laboratory | ||
| Promoter | Igh-3, immunoglobulin heavy chain 3 (serum IgG2b), mouse, laboratory | ||
| Molecular Note | The transgene construct consists of the variable and constant regions from mouse gamma2b (Igh-3) clone (VD93) fused to membrane exons from the mu (Igh-6) gene (2.6kb fragment from pVH176mu). [MGI Ref ID J:135209] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Roth PE; Doglio L; Manz JT; Kim JY; Lo D; Storb U. 1993. Immunoglobulin gamma 2b transgenes inhibit heavy chain gene rearrangement, but cannot promote B cell development. J Exp Med 178(6):2007-21. [PubMed: 8245779] [MGI Ref ID J:135209]
Additional References
Roth PE; Kurtz B; Lo D; Storb U. 1995. lambda 5, but not mu, is required for B cell maturation in a unique gamma 2b transgenic mouse line. J Exp Med 181(3):1059-70. [PubMed: 7869028] [MGI Ref ID J:23341]
Storb U; Roth P; Kurtz BK. 1994. Gamma 2b transgenic mice as a model for the role of immunoglobulins in B cell development. Immunol Res 13(4):291-8. [PubMed: 7616056] [MGI Ref ID J:135221]
Tsang H; Pinkert C; Hagman J; Lostrum M; Brinster RL; Storb U. 1988. Cloning of a gamma 2b gene encoding anti-Pseudomonas aeruginosa H chains and its introduction into the germ line of mice. J Immunol 141(1):308-14. [PubMed: 2454261] [MGI Ref ID J:135210]
Tg(Igh-3/Igh-6VD93)1241Ust relatedRoth PE; Kurtz B; Lo D; Storb U. 1995. lambda 5, but not mu, is required for B cell maturation in a unique gamma 2b transgenic mouse line. J Exp Med 181(3):1059-70. [PubMed: 7869028] [MGI Ref ID J:23341]
Storb U; Roth P; Kurtz BK. 1994. Gamma 2b transgenic mice as a model for the role of immunoglobulins in B cell development. Immunol Res 13(4):291-8. [PubMed: 7616056] [MGI Ref ID J:135221]
Tsang H; Pinkert C; Hagman J; Lostrum M; Brinster RL; Storb U. 1988. Cloning of a gamma 2b gene encoding anti-Pseudomonas aeruginosa H chains and its introduction into the germ line of mice. J Immunol 141(1):308-14. [PubMed: 2454261] [MGI Ref ID J:135210]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
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