Description

Strain Information

Former Names SJL/J-Tg(Aa26 Ab62)4Strasbourg/J    (Changed: 18-JUN-08 ) Type Congenic; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N3

Related Strains

Strains carrying   Tg(H2-Aa)26Dim allele

002081

B6.Cg-Tg(H2-Aa)26Dim Tg(H2-Ab1)62Dim/J

View Strains carrying   Tg(H2-Aa)26Dim     (1 strain)

Strains carrying   Tg(H2-Ab1)62Dim allele

002081

B6.Cg-Tg(H2-Aa)26Dim Tg(H2-Ab1)62Dim/J

View Strains carrying   Tg(H2-Ab1)62Dim     (1 strain)

Strains carrying other alleles of H2-Ab1

001162	B6(C)-H2-Ab1bm12/KhEgJ
003625	B6.C-H2-Ab1bm12/KhEg-Mc1re-J/J
002956	B6NTac.Cg-H2-Ab1tm1Gru Tg(Ab1TL)1Gru/J
006021	NOD.Cg-H2-Ab1tm1Gru Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell/EllJ
006023	NOD.Cg-H2-Ab1tm1Gru Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell/EllJ
004256	NOD.Cg-H2-Ab1tm1Gru/Dvs
004606	NOD.Cg-Prkdcscid H2-Ab1tm1Doi Tg(HLA-DQA1,HLA-DQB1)1Dv/SzJ
005589	NOD.Cg-Prkdcscid H2-Ab1tm1Doi/SzJ
006024	NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD4,HLA-DQA1,HLA-DQB1)N8Ell/EllJ
006022	NOD.Cg-Rag1tm1Mom H2-Ab1tm1Gru Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell/EllJ

View Strains carrying other alleles of H2-Ab1     (10 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(H2-Aa)26Dim/? Tg(H2-Ab1)62Dim/?

        SJL.Cg-Tg(H2-Aa)26Dim Tg(H2-Ab1)62Dim

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:110897)
  • normal B and T cell populations as determined by flow cytometry
• deviant class II histocompatibility locus (MGI Ref ID J:110897)
  • class II A^k surface expression is increased 3-10X on transgenic splenocytes

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(H2-Aa)26Dim/? Tg(H2-Ab1)62Dim/?

        B6.Cg-Tg(H2-Aa)26Dim Tg(H2-Ab1)62Dim

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:110897)
  • normal B and T cell populations as determined by flow cytometry
• abnormal antigen presentation via MHC class II (MGI Ref ID J:110897)
  • increased efficiency of antigen presentation by splenocytes in response to KLH, however, in vivo antibody response to KLH as measured by ELISA is similar for control and transgenic
• deviant class II histocompatibility locus (MGI Ref ID J:110897)
  • class II A^k surface expression is increased 3-10X on transgenic splenocytes, however, class II A^b surface expression is the same in controls and transgenics
• increased immunoglobulin level (MGI Ref ID J:110897)
  • secondary, but not primary, antibody response is increased in response to the synthetic copolymer HGAL

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(H2-Aa)26Dim
Allele Name		transgene insertion 26, Diane Mathis
Allele Type		Transgenic (random, expressed)
Common Name(s)		Aa26;
Strain of Origin		(C57BL/6 x SJL)F2
Expressed Gene		H2-Aa, histocompatibility 2, class II antigen A, alpha, mouse, laboratory
Molecular Note		A transgenic construct containing the entire mouse (AKR/J strain) H2-Aa kappa genomic DNA sequence was microinjected into (C57BL/6 x SJL)F2 embryos. Two lines Aalpha10 and Aalpha26 were generated. Line 26 was crossed to mice carrying Tg(H2-Ab1)62Dim and the resulting double transgenic line was donated to The Jackson Laboratory. The construct was also injected into C57BL/6 embryos to have the transgene on a homogeneous genetic background. Line 46 was established (Aalpha46). S1-mapping shows normal patterns of high expression in spleen, lung and thymus. [MGI Ref ID J:110897]
Allele Symbol		Tg(H2-Ab1)62Dim
Allele Name		transgene insertion 62, Diane Mathis
Allele Type		Transgenic (random, expressed)
Common Name(s)		Ab62;
Strain of Origin		(C57BL/6 x SJL)F2
Expressed Gene		H2-Ab1, histocompatibility 2, class II antigen A, beta 1, mouse, laboratory
Molecular Note		A transgenic construct containing the entire mouse (AKR/J strain) H2-Ab1 kappa genomic DNA sequence was microinjected into (C57BL/6 x SJL)F2 embryos, generating line Aalpha62. Line 62 was crossed to mice carrying Tg(H2-Aa)26Dim and the resulting double transgenic line was donated to The Jackson Laboratory. The construct was also injected into C57BL/6 embryos to have the transgene on a homogeneous genetic background. Line 42 was established (Aalpha42). S1-mapping shows normal patterns of high expression in spleen, lung and thymus. [MGI Ref ID J:110897]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Fehling HJ; van Ewijk W; Pasquali JL; Waltzinger C; Le Meur M; Gerlinger P; Benoist C; Mathis D. 1990. Functional consequences of overexpressed Ia antigens in AK alpha/AK beta transgenic mice. J Immunol 144(8):2865-74. [PubMed: 1969873]  [MGI Ref ID J:110897]

Additional References

Le Meur M; Waltzinger C; Gerlinger P; Benoist C; Mathis D. 1989. Restricted assembly of MHC class II molecules in transgenic mice. J Immunol 142(1):323-7. [PubMed: 2491873]  [MGI Ref ID J:135137]

Tg(H2-Aa)26Dim related

Le Meur M; Waltzinger C; Gerlinger P; Benoist C; Mathis D. 1989. Restricted assembly of MHC class II molecules in transgenic mice. J Immunol 142(1):323-7. [PubMed: 2491873]  [MGI Ref ID J:135137]

Tg(H2-Ab1)62Dim related

Le Meur M; Waltzinger C; Gerlinger P; Benoist C; Mathis D. 1989. Restricted assembly of MHC class II molecules in transgenic mice. J Immunol 142(1):323-7. [PubMed: 2491873]  [MGI Ref ID J:135137]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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General Terms and Conditions

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fax:	207-288-6655

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