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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names NOD/Lt-Tg(RipTAg)1Lt/J (Changed: 23-FEB-07 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation pF26+F1p Donating Investigator Edward Leiter, The Jackson Laboratory Description
Homozygous NOD/Lt-Tg(RipTag)1Lt mice develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Additional control strains are available depending on the researchers needs. Please refer to JAX Notes No. 477 for a complete list of control strains available for NOD/LtJ mice in diabetes research. Additional control strains are available depending on the researchers needs. | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Ins2-TAg)1Lt allele
002380 NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ View Strains carrying Tg(Ins2-TAg)1Lt (1 strain)
008215 (C57BL/6-Tg(TRAMP)8247Ng/J X FVB/NJ)F1/J 003382 B10.D2-Tg(C3-1-TAg)cJeg/J 008247 B6.Cg-Tg(Ela1-TAg*)289Mjt/J 003380 B6.FVB-Tg(C3-1-TAg)cJeg/J 003135 C57BL/6-Tg(TRAMP)8247Ng/J 003445 C57BL/6J-Tg(Amy1TAg)354Knw/J 003446 C57BL/6J-Tg(Amy1TAg)501Knw/J 003477 C57BL/6J-Tg(SV)419Bri/J 003476 C57BL/6J-Tg(SV)427Bri/J 002233 C57BL/6J-Tg(SV)7Bri/J 003189 C57BL/6J-Tg(WAPTAg)3Knw/J 003188 C57BL/6J-Tg(WapTAg)1Knw 005564 FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ 003381 FVB-Tg(C3-1-TAg)cJeg/J
Phenotype
Phenotype Information
assigned by genotype
Tg(Ins2-TAg)1Lt/0
NOD/ShiLt-Tg(Ins2-TAg)1Lt
- tumorigenesis
- insulinoma (MGI Ref ID J:92449)
- transgenic mice develop beta cell adenomas
This mouse can be used to support research in many areas including:
TAg relatedCancer Research
Increased Tumor Incidence
Adenomas: pancreatic b cells
Diabetes and Obesity Research
Pancreatic B Cell Adenomas
Apoptosis Research
Extracellular Modulators
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ins2-TAg)1Lt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Edward H Leiter | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(RipTAg)1Lt; | ||
| Mutation Made By | David Serreze, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | TAg, SV40 large T-antigen, SV40 | ||
| Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer. | |||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Homozygous transgenic mice on an NOD/Lt genetic background develop pancreatic beta cell adenomas. They are able to produce 2-3 litters before the insulin secreted from the adenoma makes them too hypoglycemic. | ||
| Molecular Note | The transgene contains rat insulin 2 promoter (Rip) and the wild-type allele of the SV40 large T antigen (TAg) gene. [MGI Ref ID J:92449] | ||
Genotyping
Genotyping Information
Genotyping Protocols
TAg, QPCR
TAg, SV, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hamaguchi K; Gaskins HR; Leiter EH. 1991. NIT-1, a pancreatic beta-cell line established from a transgenic NOD/Lt mouse. Diabetes 40(7):842-9. [PubMed: 1647994] [MGI Ref ID J:92449]
Additional References
Tg(Ins2-TAg)1Lt relatedSerreze DV; Chapman HD; Varnum DS; Gerling I; Leiter EH; Shultz LD. 1997. Initiation of autoimmune diabetes in NOD/Lt mice is MHC class I-dependent. J Immunol 158(8):3978-86. [PubMed: 9103469] [MGI Ref ID J:39473]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Additional control strains are available depending on the researchers needs. Please refer to JAX Notes No. 477 for a complete list of control strains available for NOD/LtJ mice in diabetes research. Additional control strains are available depending on the researchers needs. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
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Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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