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Strain Name:

B6.129P2-Apobtm1Unc/J

Stock Number:

002053

Availability:

Repository- Live

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General Terms and Conditions

Genes & Alleles   Apob;   Apobtm1Unc;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHeterozygote x +/+ sibling         (Female x Male)
Mating System+/+ sibling x Heterozygote         (Female x Male)
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129P2 via E14TG2a ES cell line
Donating Investigator Nobuyo Maeda,   Univ of North Carolina at Chapel Hill
GenerationN10F31 (07-DEC-07)

Appearance
black
Related Genotype: a/a

Strain Description
The Apobtm1Unc targeted mutation produces a truncated form of the apolipoprotein B protein (APOB70)and no apoB100 that is similar to human familial hypobetalipoproteinemia condition. Expression of apoB48 is not altered. Homozygous mice show greatly reduced levels of plasma APOB, beta-lipoproteins, and total cholesterol. They also have reduced plasma triglyceride concentrations, fasting chylomicronemia, and reduced high density lipoprotein (HDL) cholesterol. Homozygotes also have a high incidence of exencephaly and hydrocephaly. Heterozygous mice show a slight increase over wildtype in the incidence of hydrocephaly.

Strain Development
A targeting vector was designed to replace the two low density lipoprotein receptor binding domains of the endogenous exon 26 with oligonucleotides encoding human betas-globin. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Properly slected ES cells were injected into pseudopregnant females and the resultant chimeric males were mated with C57BL/6J mice. The resulting modified locus contains three repeats each containing a stop codon.

Related Disease (OMIM) Terms

Apolipoprotein B; APOB
Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Apobtm1Unc/Apob+

        involves: 129P2/OlaHsd * C57BL/6J
  • homeostasis/metabolism phenotype
  • abnormal circulating cholesterol level (MGI Ref ID J:16352)
    • reduced plasma concentration
    • decreased circulating HDL cholesterol level (MGI Ref ID J:16352)
  • abnormal triglyceride level (MGI Ref ID J:16352)
    • reduced plasma concentration

Apobtm1Unc/Apobtm1Unc

        involves: 129P2/OlaHsd * C57BL/6J
  • homeostasis/metabolism phenotype
  • abnormal circulating cholesterol level (MGI Ref ID J:16352)
    • reduced plasma concentration
    • decreased circulating HDL cholesterol level (MGI Ref ID J:16352)
  • abnormal triglyceride level (MGI Ref ID J:16352)
    • reduced plasma concentration
  • nervous system phenotype
  • exencephaly (MGI Ref ID J:16352)
    • in mice that die perinatally
    • in mice that die perinatally
  • hydroencephaly (MGI Ref ID J:16352)
    • in 30% of mice that survive until weaning
    • in 30% of mice that survive until weaning

Gene & Allele Details

Allele Symbol Apobtm1Unc
Allele Name targeted mutation 1, University of North Carolina
Mutation Made By Nobuyo Maeda,   Univ of North Carolina at Chapel Hill
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Apob, apolipoprotein B
Chromosome 12
Gene Common Name(s) AI315052; Aa1064; Ac1-060; ApoB-100; ApoB-48; FLDB; expressed sequence AI315052;
General Note Day 9 embryos show excessive cell death in the alar plate of the hind brain, possibly associated with vitamin E deficiency (J:24237).
Molecular Note An insertion-type vector was used to create mutations in exon 26 in sequences encoding a region in the encoded protein thought to be important for LDL receptor binding. An in-frame stop codon was also inserted. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB48 isoform is unaffected by this mutation, while a truncated mutant ApoB100 isoform is produced from this allele. [MGI Ref ID J:16352]

Control Information

  Allele   Control
 Apobtm1Unc  Wild-type from the colony
 Apobtm1Unc  000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Apobtm1Unc

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of Apob     (9 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           A1

Research Applications

This mouse can be used to support research in many areas including:

Apobtm1Unc related

Cardiovascular Research
Atherosclerosis
Hypocholesterolemia
Hypotriglyceridemia

Developmental Biology Research
Neural Tube Defects

Mouse/Human Gene Homologs
hypobetalipoproteinemia, familial

Neurobiology Research
Neural Tube Defects

References

Selected Reference(s)

Homanics GE; Smith TJ; Zhang SH; Lee D; Young SG; Maeda N. 1993. Targeted modification of the apolipoprotein B gene results in hypobetalipoproteinemia and developmental abnormalities in mice. Proc Natl Acad Sci U S A 90(6):2389-93. [PubMed: 8460149]  [MGI Ref ID J:16352]

Additional References

Price and Supply Information

Strain Name: B6.129P2-Apobtm1Unc/J
Stock Number: 002053

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Neural Tube Defect Resource collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
Request Form Strain from the Neural Tube Defect Resource. First time use requires submission of a Request Form, please inquire.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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