Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Background Strain C57BL/6 Donor Strain 129P2 via E14TG2a ES cell line Generation N10F36 (06-JAN-09) Generation Definitions   Donating Investigator Nobuyo Maeda,   Univ of North Carolina at Chapel Hill

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Apoa1^tm1Unc targeted mutation have no APOA1 protein in their plasma. They have severely reduced levels of plasma cholesterol and HDL-cholesterol after overnight fasting. They also show a deficiency in alpha-migrating HDL particles. The Apoa1^tm1Unc mutant mice appear to develop normally.

Development
The Apoa1^tm1Unc mutant strain was developed in the laboratory of Dr. Nobuyo Maeda at the University of North Carolina at Chapel Hill. The 129-derived E14TG2a ES cell line was used. The C57BL/6J strain was produced by backcrossing the Apoa1^tm1Unc mutation 10 times to C57BL/6J inbred mice.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Apoa1^tm1Unc/Apoa1⁺

        B6.129P2-Apoa1^tm1Unc

• homeostasis/metabolism phenotype
• decreased circulating cholesterol level
  • plasma total cholesterol is 67% of wild-type   (MGI Ref ID J:22986)

Apoa1^tm1Unc/Apoa1^tm1Unc

        B6.129P2-Apoa1^tm1Unc/J

• homeostasis/metabolism phenotype
• abnormal vascular wound healing
  • reendothelialization is delayed compared to in wild-type mice following injury to the carotid artery   (MGI Ref ID J:118063)
  • mice exhibit a 52% larger area of non-reendothelialized carotid artery following injury compared to in wild-type mice   (MGI Ref ID J:118063)
• decreased circulating cholesterol level
  • serum total cholesterol levels are reduced by one third compared to in wild-type mice   (MGI Ref ID J:114205)
  • esterified cholesterol is reduced 7-fold and free cholesterol 1.6-fold compared to in wild-type mice   (MGI Ref ID J:114205)
• • decreased circulating HDL cholesterol level
    • Apoa1-poor HDL and Apoa1-rich HDL   (MGI Ref ID J:114205)
• decreased circulating phospholipid level
  • serum phospholipids levels are reduced by one third compared to in wild-type mice   (MGI Ref ID J:114205)
• increased circulating cholesterol level
  • serum total cholesterol, free cholesterol and esterified cholesterol levels in mice treated with silver nitrate to induce an acute phase response are increased more than in similarly treated wild-type mice   (MGI Ref ID J:114205)
• increased circulating triglyceride level
  • triglyceride levels in mice treated with silver nitrate to induce an acute phase response are increased moderately compared to in similarly treated wild-type mice   (MGI Ref ID J:114205)
• cardiovascular system phenotype
• abnormal vascular wound healing
  • reendothelialization is delayed compared to in wild-type mice following injury to the carotid artery   (MGI Ref ID J:118063)
  • mice exhibit a 52% larger area of non-reendothelialized carotid artery following injury compared to in wild-type mice   (MGI Ref ID J:118063)

Apoa1^tm1Unc/Apoa1^tm1Unc

        B6.129P2-Apoa1^tm1Unc

• homeostasis/metabolism phenotype
• decreased circulating cholesterol level
  • plasma total cholesterol is 25% of wild-type   (MGI Ref ID J:22986)
  • plasma cholesteryl ester synthesis is reduced   (MGI Ref ID J:22986)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Apoa1^tm1Unc/Apoa1⁺

        involves: 129P2/OlaHsd * C57BL/6J

• homeostasis/metabolism phenotype
• decreased circulating cholesterol level
  • at 8 weeks of age, total cholesterol levels are decreased to 54% of wild-type   (MGI Ref ID J:2109)
• • decreased circulating HDL cholesterol level
    • at 8 weeks of age, HDL levels are decreased to 40% of wild-type   (MGI Ref ID J:2109)

Apoa1^tm1Unc/Apoa1^tm1Unc

        involves: 129P2/OlaHsd * C57BL/6J

• homeostasis/metabolism phenotype
• decreased circulating cholesterol level
  • at 8 weeks of age, total cholesterol levels are decreased to 33% of wild-type   (MGI Ref ID J:2109)
• • decreased circulating HDL cholesterol level
    • at 8 weeks of age, HDL levels are decreased to 17% of wild-type   (MGI Ref ID J:2109)

Apoa1^tm1Unc/Apoa1^tm1Unc

        involves: 129P2/OlaHsd

• homeostasis/metabolism phenotype
• abnormal cellular cholesterol metabolism
  • HDL cholesteryl ether clearance is over 20-fold higher than in wild-type mice and varies depending on the organ examined   (MGI Ref ID J:120501)
  • however, the clearance rate could be rescued by increasing plasma HDL cholesteryl ester levels   (MGI Ref ID J:120501)
• • decreased cholesterol efflux   (MGI Ref ID J:129136)
• decreased circulating cholesterol level   (MGI Ref ID J:129136)
• • decreased circulating HDL cholesterol level   (MGI Ref ID J:129136)
• decreased circulating phospholipid level   (MGI Ref ID J:129136)
• cellular phenotype
• abnormal cellular cholesterol metabolism
  • HDL cholesteryl ether clearance is over 20-fold higher than in wild-type mice and varies depending on the organ examined   (MGI Ref ID J:120501)
  • however, the clearance rate could be rescued by increasing plasma HDL cholesteryl ester levels   (MGI Ref ID J:120501)
• • decreased cholesterol efflux   (MGI Ref ID J:129136)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoa1^tm1Unc related

Cardiovascular Research
Hypocholesterolemia

Mouse/Human Gene Homologs
apolipoprotein A1 deficiency, amyloid polyneuropathy-nephropathy

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Apoa1tm1Unc
Allele Name		targeted mutation 1, University of North Carolina
Allele Type		Targeted (knock-out)
Common Name(s)		A1-; Apo A-1 KO; Apo A1-; ApoA-I-; apoa1tm1unc;
Mutation Made By		Nobuyo Maeda,   Univ of North Carolina at Chapel Hill
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14TG2a
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Apoa1, apolipoprotein A-I
Chromosome		9
Gene Common Name(s)		Alp-1; Apoa-1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2; apoA-I; brain protein 14; liver 20-30 thousand M.Wt protein 1; serum protein 1; serum protein 2;
Molecular Note		A genomic fragment containing exon 2 was replaced by a neomycin resistance cassette. The protein was not detectable in plasma by Ouchterlony double-immunodiffusion tests in homozygous mice. [MGI Ref ID J:2109]

Genotyping

Genotyping Information

Genotyping Protocols

Apoa1^tm1Unc, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Williamson R; Lee D; Hagaman J; Maeda N. 1992. Marked reduction of high density lipoprotein cholesterol in mice genetically modified to lack apolipoprotein A-I. Proc Natl Acad Sci U S A 89(15):7134-8. [PubMed: 1496008]  [MGI Ref ID J:2109]

Additional References

Ishiguro H; Yoshida H; Major AS; Zhu T; Babaev VR; Linton MF; Fazio S. 2001. Retrovirus-mediated expression of apolipoprotein A-I in the macrophage protects against atherosclerosis in vivo. J Biol Chem 276(39):36742-8. [PubMed: 11477092]  [MGI Ref ID J:71811]

Miettinen HE; Rayburn H; Krieger M. 2001. Abnormal lipoprotein metabolism and reversible female infertility in HDL receptor (SR-BI)-deficient mice. J Clin Invest 108(11):1717-22. [PubMed: 11733567]  [MGI Ref ID J:73138]

Parks JS; Li H; Gebre AK; Smith TL; Maeda N. 1995. Effect of apolipoprotein A-I deficiency on lecithin:cholesterol acyltransferase activation in mouse plasma. J Lipid Res 36(2):349-55. [PubMed: 7751823]  [MGI Ref ID J:22986]

Apoa1^tm1Unc related

Alam K; Meidell RS; Spady DK. 2001. Effect of up-regulating individual steps in the reverse cholesterol transport pathway on reverse cholesterol transport in normolipidemic mice. J Biol Chem 276(19):15641-9. [PubMed: 11278646]  [MGI Ref ID J:69417]

Boisvert WA; Black AS; Curtiss LK. 1999. ApoA1 reduces free cholesterol accumulation in atherosclerotic lesions of ApoE-deficient mice transplanted with ApoE-expressing macrophages. Arterioscler Thromb Vasc Biol 19(3):525-30. [PubMed: 10073953]  [MGI Ref ID J:55595]

Cabana VG; Feng N; Reardon CA; Lukens J; Webb NR; de Beer FC; Getz GS. 2004. Influence of apoA-I and apoE on the formation of serum amyloid A-containing lipoproteins in vivo and in vitro. J Lipid Res 45(2):317-25. [PubMed: 14595002]  [MGI Ref ID J:121045]

Cabana VG; Reardon CA; Wei B; Lukens JR; Getz GS. 1999. SAA-only HDL formed during the acute phase response in apoA-I+/+ and apoA-I-/- mice. J Lipid Res 40(6):1090-103. [PubMed: 10357841]  [MGI Ref ID J:55744]

Callow MJ; Dudoit S; Gong EL; Speed TP; Rubin EM. 2000. Microarray expression profiling identifies genes with altered expression in HDL-deficient mice. Genome Res 10(12):2022-9. [PubMed: 11116096]  [MGI Ref ID J:115384]

Chiesa G; Parolini C; Canavesi M; Colombo N; Sirtori CR; Fumagalli R; Franceschini G; Bernini F. 1998. Human apolipoproteins A-I and A-II in cell cholesterol efflux: studies with transgenic mice. Arterioscler Thromb Vasc Biol 18(9):1417-23. [PubMed: 9743230]  [MGI Ref ID J:129136]

Chroni A; Duka A; Kan HY; Liu T; Zannis VI. 2005. Point mutations in apolipoprotein A-I mimic the phenotype observed in patients with classical lecithin:cholesterol acyltransferase deficiency. Biochemistry 44(43):14353-66. [PubMed: 16245952]  [MGI Ref ID J:105606]

Combes V; Coltel N; Alibert M; van Eck M; Raymond C; Juhan-Vague I; Grau GE; Chimini G. 2005. ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology. Am J Pathol 166(1):295-302. [PubMed: 15632021]  [MGI Ref ID J:95235]

Fagan AM; Christopher E; Taylor JW; Parsadanian M; Spinner M; Watson M; Fryer JD; Wahrle S; Bales KR; Paul SM; Holtzman DM. 2004. ApoAI deficiency results in marked reductions in plasma cholesterol but no alterations in amyloid-beta pathology in a mouse model of Alzheimer's disease-like cerebral amyloidosis. Am J Pathol 165(4):1413-22. [PubMed: 15466405]  [MGI Ref ID J:93658]

Goodrum JF; Bouldin TW; Zhang SH; Maeda N; Popko B. 1995. Nerve regeneration and cholesterol reutilization occur in the absence of apolipoproteins E and A-I in mice. J Neurochem 64(1):408-16. [PubMed: 7798939]  [MGI Ref ID J:22362]

Gruen ML; Plummer MR; Zhang W; Posey KA; Linton M; Fazio S; Hasty AH. 2005. Persistence of high density lipoprotein particles in obese mice lacking apolipoprotein A-I. J Lipid Res 46(9):2007-14. [PubMed: 15995171]  [MGI Ref ID J:100492]

Hajri T; Elliott-Bryant R; Sipe JD; Liang JS; Hayes KC; Cathcart ES. 1998. The acute phase response in apolipoprotein A-1 knockout mice: apolipoprotein serum amyloid A and lipid distribution in plasma high density lipoproteins. Biochim Biophys Acta 1394(2-3):209-18. [PubMed: 9795222]  [MGI Ref ID J:114205]

Hirsch-Reinshagen V; Zhou S; Burgess BL; Bernier L; McIsaac SA; Chan JY; Tansley GH; Cohn JS; Hayden MR; Wellington CL. 2004. Deficiency of ABCA1 impairs apolipoprotein E metabolism in brain. J Biol Chem 279(39):41197-207. [PubMed: 15269218]  [MGI Ref ID J:93331]

Hopkins PC; Huang Y; McGuire JG; Pitas RE. 2002. Evidence for differential effects of apoE3 and apoE4 on HDL metabolism. J Lipid Res 43(11):1881-9. [PubMed: 12401887]  [MGI Ref ID J:123999]

Hughes SD; Verstuyft J; Rubin EM. 1997. HDL deficiency in genetically engineered mice requires elevated LDL to accelerate atherogenesis. Arterioscler Thromb Vasc Biol 17(9):1725-9. [PubMed: 9327769]  [MGI Ref ID J:43915]

Iqbal J; Hussain MM. 2005. Evidence for multiple complementary pathways for efficient cholesterol absorption in mice. J Lipid Res 46(7):1491-501. [PubMed: 15834127]  [MGI Ref ID J:100497]

Ishii T; Hasegawa T; Pai CI; Yvgi-Ohana N; Timberg R; Zhao L; Majdic G; Chung BC; Orly J; Parker KL. 2002. The roles of circulating high-density lipoproteins and trophic hormones in the phenotype of knockout mice lacking the steroidogenic acute regulatory protein. Mol Endocrinol 16(10):2297-309. [PubMed: 12351695]  [MGI Ref ID J:79229]

Ji Y; Wang N; Ramakrishnan R; Sehayek E; Huszar D; Breslow JL; Tall AR. 1999. Hepatic scavenger receptor BI promotes rapid clearance of high density lipoprotein free cholesterol and its transport into bile. J Biol Chem 274(47):33398-402. [PubMed: 10559220]  [MGI Ref ID J:115122]

Jolley CD; Dietschy JM; Turley SD. 2000. Induction of bile acid synthesis by cholesterol and cholestyramine feeding is unimpaired in mice deficient in apolipoprotein AI. Hepatology 32(6):1309-16. [PubMed: 11093738]  [MGI Ref ID J:87499]

Komori H; Arai H; Kashima T; Huby T; Kita T; Ueda Y. 2008. Coexpression of CLA-1 and human PDZK1 in murine liver modulates HDL cholesterol metabolism. Arterioscler Thromb Vasc Biol 28(7):1298-303. [PubMed: 18403724]  [MGI Ref ID J:159814]

Kypreos KE. 2008. ABCA1 promotes the de novo biogenesis of apolipoprotein CIII-containing HDL particles in vivo and modulates the severity of apolipoprotein CIII-induced hypertriglyceridemia. Biochemistry 47(39):10491-502. [PubMed: 18767813]  [MGI Ref ID J:141173]

Kypreos KE; Zannis VI. 2007. Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCAT. Biochem J 403(2):359-67. [PubMed: 17206937]  [MGI Ref ID J:121679]

Lee M; Calabresi L; Chiesa G; Franceschini G; Kovanen PT. 2002. Mast cell chymase degrades apoE and apoA-II in apoA-I-knockout mouse plasma and reduces its ability to promote cellular cholesterol efflux. Arterioscler Thromb Vasc Biol 22(9):1475-81. [PubMed: 12231569]  [MGI Ref ID J:102936]

Lefterov I; Fitz NF; Cronican AA; Fogg A; Lefterov P; Kodali R; Wetzel R; Koldamova R. 2010. Apolipoprotein A-I deficiency increases cerebral amyloid angiopathy and cognitive deficits in APP/PS1DeltaE9 mice. J Biol Chem 285(47):36945-57. [PubMed: 20739292]  [MGI Ref ID J:166877]

Li H; Reddick RL; Maeda N. 1993. Lack of apoA-I is not associated with increased susceptibility to atherosclerosis in mice. Arterioscler Thromb 13(12):1814-21. [PubMed: 8241102]  [MGI Ref ID J:28517]

Major AS; Dove DE; Ishiguro H; Su YR; Brown AM; Liu L; Carter KJ; Linton MF; Fazio S. 2001. Increased cholesterol efflux in apolipoprotein AI (ApoAI)-producing macrophages as a mechanism for reduced atherosclerosis in ApoAI((-/-)) mice. Arterioscler Thromb Vasc Biol 21(11):1790-5. [PubMed: 11701467]  [MGI Ref ID J:107390]

Moore RE; Navab M; Millar JS; Zimetti F; Hama S; Rothblat GH; Rader DJ. 2005. Increased atherosclerosis in mice lacking apolipoprotein A-I attributable to both impaired reverse cholesterol transport and increased inflammation. Circ Res 97(8):763-71. [PubMed: 16151025]  [MGI Ref ID J:114636]

Murphy AJ; Akhtari M; Tolani S; Pagler T; Bijl N; Kuo CL; Wang M; Sanson M; Abramowicz S; Welch C; Bochem AE; Kuivenhoven JA; Yvan-Charvet L; Tall AR. 2011. ApoE regulates hematopoietic stem cell proliferation, monocytosis, and monocyte accumulation in atherosclerotic lesions in mice. J Clin Invest 121(10):4138-49. [PubMed: 21968112]  [MGI Ref ID J:178453]

Norata GD; Callegari E; Marchesi M; Chiesa G; Eriksson P; Catapano AL. 2005. High-density lipoproteins induce transforming growth factor-beta2 expression in endothelial cells. Circulation 111(21):2805-11. [PubMed: 15911702]  [MGI Ref ID J:112255]

Norata GD; Marchesi P; Pirillo A; Uboldi P; Chiesa G; Maina V; Garlanda C; Mantovani A; Catapano AL. 2008. Long pentraxin 3, a key component of innate immunity, is modulated by high-density lipoproteins in endothelial cells. Arterioscler Thromb Vasc Biol 28(5):925-31. [PubMed: 18218986]  [MGI Ref ID J:159816]

Parks JS; Li H; Gebre AK; Smith TL; Maeda N. 1995. Effect of apolipoprotein A-I deficiency on lecithin:cholesterol acyltransferase activation in mouse plasma. J Lipid Res 36(2):349-55. [PubMed: 7751823]  [MGI Ref ID J:22986]

Quan G; Xie C; Dietschy JM; Turley SD. 2003. Ontogenesis and regulation of cholesterol metabolism in the central nervous system of the mouse. Brain Res Dev Brain Res 146(1-2):87-98. [PubMed: 14643015]  [MGI Ref ID J:86935]

Seetharam D; Mineo C; Gormley AK; Gibson LL; Vongpatanasin W; Chambliss KL; Hahner LD; Cummings ML; Kitchens RL; Marcel YL; Rader DJ; Shaul PW. 2006. High-density lipoprotein promotes endothelial cell migration and reendothelialization via scavenger receptor-B type I. Circ Res 98(1):63-72. [PubMed: 16339487]  [MGI Ref ID J:118063]

Sorci-Thomas MG; Thomas M; Curtiss L; Landrum M. 2000. Single repeat deletion in ApoA-I blocks cholesterol esterification and results in rapid catabolism of delta6 and wild-type ApoA-I in transgenic mice. J Biol Chem 275(16):12156-63. [PubMed: 10766851]  [MGI Ref ID J:61733]

Spady DK; Woollett LA; Meidell RS; Hobbs HH. 1998. Kinetic characteristics and regulation of HDL cholesteryl ester and apolipoprotein transport in the apoA-I-/- mouse. J Lipid Res 39(7):1483-92. [PubMed: 9684752]  [MGI Ref ID J:120501]

Suzuki M; Pritchard DK; Becker L; Hoofnagle AN; Tanimura N; Bammler TK; Beyer RP; Bumgarner R; Vaisar T; de Beer MC; de Beer FC; Miyake K; Oram JF; Heinecke JW. 2010. High-density lipoprotein suppresses the type I interferon response, a family of potent antiviral immunoregulators, in macrophages challenged with lipopolysaccharide. Circulation 122(19):1919-27. [PubMed: 20974999]  [MGI Ref ID J:179479]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Wang W; Xu H; Shi Y; Nandedkar S; Zhang H; Gao H; Feroah T; Weihrauch D; Schulte ML; Jones DW; Jarzembowski J; Sorci-Thomas M; Pritchard KA Jr. 2010. Genetic deletion of apolipoprotein A-I increases airway hyperresponsiveness, inflammation, and collagen deposition in the lung. J Lipid Res 51(9):2560-70. [PubMed: 20498409]  [MGI Ref ID J:164463]

Wang Y; X738b X8000 X52c7; Sawashita J; X6fa4 X4e0b X4ec1 X5b50; Qian J; X94b1 X91d1 X6cfd; Zhang B; X5f20 X84d3 X8339; Fu X; X4ed8 X7b11 X5f71; Tian G; X7530 X8015; Chen L; X9648 X78ca; Mori M; X68ee X653f X4e4b; Higuchi K; X6a0b X53e3 X4eac X4e00. 2011. ApoA-I deficiency in mice is associated with redistribution of apoA-II and aggravated AApoAII amyloidosis. J Lipid Res 52(8):1461-1470. [PubMed: 21622630]  [MGI Ref ID J:174979]

Webb NR; de Beer MC; Asztalos BF; Whitaker N; van der Westhuyzen DR; de Beer FC. 2004. Remodeling of HDL remnants generated by scavenger receptor class B type I. J Lipid Res 45(9):1666-73. [PubMed: 15210842]  [MGI Ref ID J:93254]

Webb NR; de Beer MC; van der Westhuyzen DR; Kindy MS; Banka CL; Tsukamoto K; Rader DL; de Beer FC. 1997. Adenoviral vector-mediated overexpression of serum amyloid A in apoA-I-deficient mice. J Lipid Res 38(8):1583-90. [PubMed: 9300780]  [MGI Ref ID J:42632]

Wilhelm AJ; Zabalawi M; Grayson JM; Weant AE; Major AS; Owen J; Bharadwaj M; Walzem R; Chan L; Oka K; Thomas MJ; Sorci-Thomas MG. 2009. Apolipoprotein A-I and its role in lymphocyte cholesterol homeostasis and autoimmunity. Arterioscler Thromb Vasc Biol 29(6):843-9. [PubMed: 19286630]  [MGI Ref ID J:162193]

Wilhelm AJ; Zabalawi M; Owen JS; Shah D; Grayson JM; Major AS; Bhat S; Gibbs DP Jr; Thomas MJ; Sorci-Thomas MG. 2010. Apolipoprotein A-I modulates regulatory T cells in autoimmune LDLr-/-, ApoA-I-/- mice. J Biol Chem 285(46):36158-69. [PubMed: 20833724]  [MGI Ref ID J:166862]

Zabalawi M; Bharadwaj M; Horton H; Cline M; Willingham M; Thomas MJ; Sorci-Thomas MG. 2007. Inflammation and skin cholesterol in LDLr-/-, apoA-I-/- mice: link between cholesterol homeostasis and self-tolerance? J Lipid Res 48(1):52-65. [PubMed: 17071966]  [MGI Ref ID J:117480]

Zabalawi M; Bhat S; Loughlin T; Thomas MJ; Alexander E; Cline M; Bullock B; Willingham M; Sorci-Thomas MG. 2003. Induction of fatal inflammation in LDL receptor and ApoA-I double-knockout mice fed dietary fat and cholesterol. Am J Pathol 163(3):1201-13. [PubMed: 12937162]  [MGI Ref ID J:85174]

de Beer MC; van der Westhuyzen DR; Whitaker NL; Webb NR; de Beer FC. 2005. SR-BI-mediated selective lipid uptake segregates apoA-I and apoA-II catabolism. J Lipid Res 46(10):2143-50. [PubMed: 16061955]  [MGI Ref ID J:104721]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This Apoa1tm1Unc strain is maintained by homozygous sibling matings. Homozygous mice may be ordered. Expected coat color from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls

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• This strain is included in the Induced Mutant Resource Colony collection.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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