Strain Name:

C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ

Stock Number:

002063

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names C57BL/6JEi-Chr YAKR Tg(Sry)1Ei    (Changed: 16-MAY-07 )
C57BL/6JEi-YAKR TgN(Sry)1Ei    (Changed: 15-DEC-04 )
Type Consomic;
Additional information on Consomic Mice.
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationB6JEi-YN31+N3
 
Donating Investigator Eva Eicher,   The Jackson Laboratory

Description
In this strain, XX mice that carry the Tg(Sry)1Ei transgene are sex-reversed males. XYAKR/J mice heterozygous for TOrl on a C57BL/6J background develop as sex reversed females, but in the presence of the Tg(Sry)1Ei transgene these XY mice develop as normal males with testes.

Development
The 129-derived 14.6-kb of genomic sequence encoding Sry, described by Gubbay et al. (Nature, 1990. v346:245), was injected into the male pronuclei of fertilized eggs obtained from mating C57BL/6JEi females with C57BL/6JEi-Chr YAKR/J males. Three XY transgenic male founders were obtained and each generated a line that was maintained by backcrossing transgenic males, obligatorily carrying the consomic Chr YAKR/J, to C57BL/6JEi females (Eicher et al., 2001). All three strains were independently cryopreserved.

Related Strains

View Other Related Strains     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(Sry)1Ei/0

        C57BL/6JEi-Chr YAKR
  • reproductive system phenotype
  • infertility (MGI Ref ID J:71077)
    • chromosomal females develop as males but are sterile due to the presence of 2 X chromosomes and the absence of a Y chromosome
  • primary sex reversal (MGI Ref ID J:71077)
    • chromosomal females carrying this transgene develop as males
    • chromosomal males that would have developed as sex-reversed females develop as males when carrying this transgene

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

TOrl/T+ Tg(Sry)1Ei/0

        B6.Cg-TOrl/EiJ
  • reproductive system phenotype
  • primary sex reversal (MGI Ref ID J:71077)
    • chromosomal females carrying this transgene develop as males
    • chromosomal males with a C57BL/6 background and an AKR Y chromosome develop as sex-reversed female; in the presence of this transgene testes develop
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tg(Sry)1Ei related

Developmental Biology Research
Internal/Organ Defects (gonads)

Reproductive Biology Research
Developmental Defects Affecting Gonads

Research Tools
Developmental Biology Research
Reproductive Biology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Sry)1Ei
Allele Name transgene insertion 1, Eva M Eicher
Allele Type Transgenic (random, expressed)
Common Name(s) Tg(Sry-129)1Ei;
Strain of OriginC57BL/6JEi-Chr YAKR
General Note Three transgenic lines (referred to as TgN(Sry-129)1Ei, TgN(Sry-129)2Ei, and TgN(Sry-129)3Ei were generated which all produced sex-reversed XX transgenic male offspring. No copy number or insertion location information was provided.
Molecular Note This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. [MGI Ref ID J:71077]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455]  [MGI Ref ID J:71077]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery of Strains Needing Progeny Testing.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent.

    Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

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