Description

Strain Information

Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Progeny Tested         (Female x Male) Species laboratory mouse Generation N7F34 (07-DEC-07)

Appearance
albino
Related Genotype: A/A Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c

Description
Mice homozygous for the male sterility and histoincompatibility spontaneous mutation (mshi) are characterized by reduced testis size and sterility. The testes are highly disorganized and appear to have a block in the regulation of male germ cell proliferation. Reproduction is normal in homozygous mutant female mice. In addition to the male reproductive defects this mutation also affects histocompatibility in both sexes. Most homozygous mutant mice reject sex-matched skin grafts from BALB/cBy mice.

Control Information

	Control
	Untyped from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

mshi/mshi

        BALB/cBy

• reproductive system phenotype
• abnormal seminiferous tubule morphology (MGI Ref ID J:37165)
  • the tubules are vacuolated with inadequate tubule lumina development
• Leydig cell hyperplasia (MGI Ref ID J:37165)
  • leydig cells occupy relatively more of the testes in homozygotes than in age-matched wild-type controls
• abnormal Sertoli cell morphology (MGI Ref ID J:37165)
  • the Sertoli cells are displaced from their normal position in homozygotes
• abnormal seminiferous epithelium morphology (MGI Ref ID J:37165)
  • the seminiferous epithelium is disorganized
• arrest of male meiosis (MGI Ref ID J:37165)
  • by 3 weeks a delay in the onset of spermatogenesis is apparent and by 5 weeks greatly reduced numbers of spermatogenic cells are seen in homozygotes
  • the spermatogenic cells that are present do not progress past the meiotic spermatocyte stages
• male infertility (MGI Ref ID J:37165)
  • males are completely sterile
• immune system phenotype
• decreased length of allograft survival (MGI Ref ID J:37165)
  • homozygous mice reject sex-matched skin grafts from congenic BALB/cBy mice unlike wild-type mice
• endocrine/exocrine gland phenotype
• abnormal seminiferous tubule morphology (MGI Ref ID J:37165)
  • the tubules are vacuolated with inadequate tubule lumina development
• Leydig cell hyperplasia (MGI Ref ID J:37165)
  • leydig cells occupy relatively more of the testes in homozygotes than in age-matched wild-type controls
• abnormal Sertoli cell morphology (MGI Ref ID J:37165)
  • the Sertoli cells are displaced from their normal position in homozygotes
• abnormal seminiferous epithelium morphology (MGI Ref ID J:37165)
  • the seminiferous epithelium is disorganized

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

mshi related

Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects (males only)

Genes & Alleles

Gene & Allele Information

Allele Symbol		mshi
Allele Name		male sterility and histoincompatibility
Allele Type		Spontaneous
Strain of Origin		BALB/cBy

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Rule MC; Mutcherson RJ 2nd; Foss AD; Nguyen TK; Myrie KA; King TR. 1999. Mouse male sterility and histoincompatibility (mshi) maps between the D10Mit51/168/212 cluster and D10Mit213. Mamm Genome 10(5):447-50. [PubMed: 10337616]  [MGI Ref ID J:54499]

Ward-Bailey PF; Johnson KR; Handel MA; Harris BS; Davisson MT. 1996. A new mouse mutation causing male sterility and histoincompatibility. Mamm Genome 7(11):793-7. [PubMed: 8875885]  [MGI Ref ID J:37165]

Additional References

mshi related

Clemente EJ; Furlong RA; Loveland KL; Affara NA. 2006. Gene expression study in the juvenile mouse testis: identification of stage-specific molecular pathways during spermatogenesis. Mamm Genome 17(9):956-75. [PubMed: 16964443]  [MGI Ref ID J:112872]

Rabionet M; van der Spoel AC; Chuang CC; von Tumpling-Radosta B; Litjens M; Bouwmeester D; Hellbusch CC; Korner C; Wiegandt H; Gorgas K; Platt FM; Grone HJ; Sandhoff R. 2008. Male germ cells require polyenoic sphingolipids with complex glycosylation for completion of meiosis: a link to ceramide synthase-3. J Biol Chem 283(19):13357-69. [PubMed: 18308723]  [MGI Ref ID J:137091]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Colony Maintenance

Mating System	Progeny Tested         (Female x Male)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Untyped from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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