Strain Name:

B6.129P2-Hprtb-m3/J

Stock Number:

002171

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Mutant mice have no HPRT detectable by Western blot analysis and no detectable HPRT enzyme activity in brain homogenates. They appear to have normal brain purine content, but de novo purine synthesis is accelerated four to five-fold. The Hprtb-m3 mutation has been used in preimplantation studies to determine when the maternal and paternal alleles of Hprt are activated during early embryonic development. However, the null mutant does not show the characteristics of Lesch-Nyhan disease.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129P2-Hprtb-m3/J    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129P2 via E14TG2a ES cell line
 
Donating InvestigatorDr. Ted Friedman,   UCSD School of Medicine

Appearance
black
Related Genotype: a/a

Description
HPRT - embryonic stem cells were obtained by selecting for spontaneous mutation by incubation in medium containing 6-thioguanine. HPRT - males have no overt phenotype of abnormal behavior. The mutation is due to a large deletion in the Hprt gene. Mutant mice have no HPRT detectable by Western blot analysis and no detectable HPRT enzyme activity in brain homogenates. They appear to have normal brain purine content, but de novo purine synthesis is accelerated four to five-fold. The Hprtb-m3 mutation has been used in preimplantation studies to determine when the maternal and paternal alleles of Hprt are activated during early embryonic development. Either administration of amphetamine or inhibition of adenine phosphoribosyltransferase (APRT) activity stimulates locomotor and stereotypic behaviors in HPRT-deficient mice. However, the null mutant for both Hprt and Aprt does not show the characteristics of Lesch-Nyhan disease.

Development
HPRT - embryonic stem cells, derived from the E14TG2a ES cell line, were obtained by selecting for spontaneous mutation by incubation in medium containing 6-thioguanine. Selected ES stem cells were implanted into (C57BL/6Lac X CBA/CaLac) F1 host blastula, and injected blastocysts were fostered in (C57BL/6JLac X CBA/CaLac) F1 females. Germline transmitting chimeras were then crossed with BALB/c mice, and the line was subsequently backcrossed to C57BL/6J for 18 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Hprtb-m3 allele
012480   NOD.Cg-Prkdcscid Il2rgtm1Wjl Hprtb-m3/EshJ
View Strains carrying   Hprtb-m3     (1 strain)

Strains carrying other alleles of Hprt
004302   129S1/Sv-Hprttm1(cre)Mnn/J
002027   129S8/SvEv-Gpi1c Hprtb-m2/J
008710   B6.129P2(129S4)-Hprttm10(Ple162-EGFP/cre)Ems/Mmjax
008877   B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax
009114   B6.129P2(129S4)-Hprttm14(Ple103-EGFP/cre)Ems/Mmjax
009116   B6.129P2(129S4)-Hprttm16(Ple167-EGFP/cre)Ems/Mmjax
008709   B6.129P2(129S4)-Hprttm9(Ple178-EGFP/cre)Ems/Mmjax
009113   B6.129P2(Cg)-Hprttm13(Ple54-EGFP)Ems/Mmjax
009115   B6.129P2(Cg)-Hprttm15(Ple111-EGFP)Ems/Mmjax
009348   B6.129P2(Cg)-Hprttm17(Ple48-lacZ)Ems/Mmjax
009118   B6.129P2(Cg)-Hprttm18(Ple90-EGFP)Ems/Mmjax
012572   B6.129P2(Cg)-Hprttm19(Ple88-lacZ)Ems/Mmjax
009353   B6.129P2(Cg)-Hprttm20(Ple53-EGFP)Ems/Mmjax
009596   B6.129P2(Cg)-Hprttm33(Ple183-EGFP)Ems/Mmjax
010770   B6.129P2(Cg)-Hprttm34(Ple186-EGFP)Ems/Mmjax
012574   B6.129P2(Cg)-Hprttm38(Ple17-lacZ)Ems/Mmjax
012575   B6.129P2(Cg)-Hprttm39(Ple24-lacZ)Ems/Mmjax
008706   B6.129P2(Cg)-Hprttm4(Ple88-EGFP)Ems/Mmjax
012576   B6.129P2(Cg)-Hprttm40(Ple34-lacZ)Ems/Mmjax
010805   B6.129P2(Cg)-Hprttm41(Ple160-lacZ)Ems/Mmjax
012331   B6.129P2(Cg)-Hprttm42(Ple131-lacZ)Ems/Mmjax
012577   B6.129P2(Cg)-Hprttm43(Ple140-lacZ)Ems/Mmjax
010709   B6.129P2(Cg)-Hprttm44(Ple49-lacZ)Ems/Mmjax
012333   B6.129P2(Cg)-Hprttm45(Ple67-lacZ)Ems/Mmjax
012733   B6.129P2(Cg)-Hprttm53(CAG-lacZ)Ems/Mmjax
010789   B6.129P2(Cg)-Hprttm54(Ple233-EGFP)Ems/Mmjax
012578   B6.129P2(Cg)-Hprttm56(Ple25-lacZ)Ems/Mmjax
012579   B6.129P2(Cg)-Hprttm58(Ple119-lacZ)Ems/Mmjax
012580   B6.129P2(Cg)-Hprttm59(Ple123-lacZ)Ems/Mmjax
012581   B6.129P2(Cg)-Hprttm62(Ple153-lacZ)Ems/Mmjax
012342   B6.129P2(Cg)-Hprttm63(Ple12-lacZ)Ems/Mmjax
012347   B6.129P2(Cg)-Hprttm64(Ple170-lacZ)Ems/Mmjax
012582   B6.129P2(Cg)-Hprttm67(Ple238-lacZ)Ems/Mmjax
012583   B6.129P2(Cg)-Hprttm68(Ple127-lacZ)Ems/Mmjax
008707   B6.129P2(Cg)-Hprttm7(Ple185-EGFP)Ems/Mmjax
012656   B6.129P2(Cg)-Hprttm70(Ple240-lacZ)Ems/Mmjax
012657   B6.129P2(Cg)-Hprttm71(Ple155-lacZ)Ems/Mmjax
012659   B6.129P2(Cg)-Hprttm73(Ple142-lacZ)Ems/Mmjax
012734   B6.129P2(Cg)-Hprttm74(Ple232-lacZ)Ems/Mmjax
008708   B6.129P2(Cg)-Hprttm8(Ple151-EGFP)Ems/Mmjax
023678   B6.Cg-Hprttm333(Ple281-icre/ERT2)Ems/Mmjax
023685   B6.Cg-Hprttm340(Ple252-icre/ERT2)Ems/Mmjax
023686   B6.Cg-Hprttm341(Ple273-icre/ERT2)Ems/Mmjax
023688   B6.Cg-Hprttm343(Ple270-icre/ERT2)Ems/Mmjax
014536   B6.Cg-Hprttm75(Ple143-lacZ)Ems/Mmjax
021427   B6;129-Hprttm1(CMV-GFP)Nat/J
003138   B6;129-Hprttm1Detl/J
021428   B6;129-Hprttm2(CMV-tdTomato)Nat/J
000807   RBJ/DnJ
024746   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Hprttm1(tetO-Dkk1)Spdl Tg(TCF/Lef1-lacZ)34Efu/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
009349   STOCK Hprttm31(Ple67-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
010707   STOCK Hprttm37(lacZ)Ems/Mmjax
012335   STOCK Hprttm50(Ple55-lacZ)Ems/Mmjax
013764   STOCK Hprttm57(Ple26-lacZ)Ems/Mmjax
012353   STOCK Hprttm65(Ple53-lacZ)Ems/Mmjax
012354   STOCK Hprttm66(Ple5-lacZ)Ems/Mmjax
012584   STOCK Hprttm69(Ple134-lacZ)Ems/Mmjax
View Strains carrying other alleles of Hprt     (59 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Lesch-Nyhan Syndrome; LNS
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Kelley-Seegmiller Syndrome   (HPRT1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Hprtb-m3/Y

        B6.129P2-Hprtb-m3
  • nervous system phenotype
  • abnormal dopaminergic neuron morphology
    • dopaminergic neurons from E14 Hprt-deficient mice differentiate in culture, but dendrite outgrowth is decelerated and the average dendrite length per neuron is lower than in control neurons; the difference in length increased over time in culture with the deficit being 15% after 2 days in culture to 32% at day 8   (MGI Ref ID J:107966)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Hprtb-m3/Y

        involves: 129P2/OlaHsd
  • behavior/neurological phenotype
  • abnormal sleep pattern
    • mice spend less time sleeping than wild-type mice   (MGI Ref ID J:3354)
  • increased grooming behavior
    • mice exhibit increased grooming time and trauma to the ears and flanks compared to in wild-type mice   (MGI Ref ID J:3354)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hprtb-m3 related

Metabolism Research

Neurobiology Research
Neurodegeneration

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Hprtb-m3
Allele Name hypoxanthine guanine phosphoribosyl transferase B, mutation 3
Allele Type Spontaneous
Common Name(s) Hprt-;
Mutation Made By Elizabeth Simpson,   Centre for Molecular Medicine & Therapeutics, University of British Columbia
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Hprt, hypoxanthine guanine phosphoribosyl transferase
Chromosome X
Gene Common Name(s) C81579; HGPRT; Hgprtase; Hprt1; expressed sequence C81579; hypoxanthine guanine phosphoribosyl transferase 1;
General Note HPRT- embryonic stem cells were obtained by selecting for spontaneous mutation by incubation in medium containing 6-thioguanine. HPRT- males have no overt phenotype of abnormal behavior (J:15483). The mutation is due to a large deletion in the Hprt gene.In situ hybridization studies showed HPRT mRNA in high levels in most neurons, but not in glial cells, in normal mice. No HPRT mRNA was detected in the brains of male mice carrying this deletion (J:2058). Mutant mice have no HPRT detectable by Western blot analysis and no detectable HPRT enzyme activity in brain homogenates. They appear to have normal brain purine content, but de novo purine synthesis is accelerated four- to fivefold (J:11842). The Hprtb-m3 mutation has been used in preimplantation studies to determine when the maternal and paternal alleles of Hprt are activated during early embryonic development (J:2389). Either administration of amphetamine (J:1847) or inhibition of adenine phosphoribosyltransferase (APRT) activity (J:4123) stimulates locomotor and stereotypic behaviors in HPRT-deficient mice. However, the null mutant for both Hprt and Aprt does not show the characteristics of Lesch-Nyhan disease (J:35822).Cells from mice hemizygous or homozygous for this mutation are HPRT-deficient and resistant to the drug 6-thioguanine (6TG).
Molecular Note The allele contains a ~55 kb deletion spanning the promoter and first 2 exons. Subsequent direct sequence comparison with wild type DNA defined the exact breakpoints of the deletion, which lies 415 bp after the 3' end of exon 2, and determined the deletion size to be 36 kb. [MGI Ref ID J:144244] [MGI Ref ID J:41459]

Genotyping

Genotyping Information

Genotyping Protocols

Hprtb-m3, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hooper M; Hardy K; Handyside A; Hunter S; Monk M. 1987. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature 326(6110):292-5. [PubMed: 3821905]  [MGI Ref ID J:15483]

Additional References

Gu JJ; Tolin AK; Jain J; Huang H; Santiago L; Mitchell BS. 2003. Targeted disruption of the inosine 5'-monophosphate dehydrogenase type I gene in mice. Mol Cell Biol 23(18):6702-12. [PubMed: 12944494]  [MGI Ref ID J:85443]

Jinnah HA; Gage FH; Friedmann T. 1991. Amphetamine-induced behavioral phenotype in a hypoxanthine-guanine phosphoribosyltransferase-deficient mouse model of Lesch-Nyhan syndrome. Behav Neurosci 105(6):1004-12. [PubMed: 1777100]  [MGI Ref ID J:1847]

Jinnah HA; Page T; Friedmann T. 1993. Brain purines in a genetic mouse model of Lesch-Nyhan disease. J Neurochem 60(6):2036-45. [PubMed: 8492116]  [MGI Ref ID J:11842]

Moore TF; Whittingham DG. 1992. Imprinting of phosphoribosyltransferases during preimplantation development of the mouse mutant, Hprtb-m3. Development 115(4):1011-6. [PubMed: 1451655]  [MGI Ref ID J:2389]

Wu CL; Melton DW. 1993. Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet 3(3):235-40. [PubMed: 8485579]  [MGI Ref ID J:4123]

Hprtb-m3 related

Boer P; Brosh S; Wasserman L; Hammel I; Zoref-Shani E; Sperling O. 2001. Decelerated rate of dendrite outgrowth from dopaminergic neurons in primary cultures from brains of hypoxanthine phosphoribosyltransferase-deficient knockout mice. Neurosci Lett 303(1):45-8. [PubMed: 11297820]  [MGI Ref ID J:107966]

Egami K; Yitta S; Kasim S; Lewers JC; Roberts RC; Lehar M; Jinnah HA. 2007. Basal ganglia dopamine loss due to defect in purine recycling. Neurobiol Dis 26(2):396-407. [PubMed: 17374562]  [MGI Ref ID J:134847]

Engle SJ; Womer DE; Davies PM; Boivin G; Sahota A; Simmonds HA; Stambrook PJ; Tischfield JA. 1996. HPRT-APRT-deficient mice are not a model for lesch-nyhan syndrome. Hum Mol Genet 5(10):1607-10. [PubMed: 8894695]  [MGI Ref ID J:35822]

Gu JJ; Stegmann S; Gathy K; Murray R; Laliberte J; Ayscue L; Mitchell BS. 2000. Inhibition of T lymphocyte activation in mice heterozygous for loss of the IMPDH II gene. J Clin Invest 106(4):599-606. [PubMed: 10953035]  [MGI Ref ID J:86045]

Gu JJ; Tolin AK; Jain J; Huang H; Santiago L; Mitchell BS. 2003. Targeted disruption of the inosine 5'-monophosphate dehydrogenase type I gene in mice. Mol Cell Biol 23(18):6702-12. [PubMed: 12944494]  [MGI Ref ID J:85443]

Guibinga GH; Murray F; Barron N; Pandori W; Hrustanovic G. 2013. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22(22):4502-15. [PubMed: 23804752]  [MGI Ref ID J:202270]

Hacke K; Szakmary A; Cuddihy AR; Rozengurt N; Lemp NA; Aubrecht J; Lawson GW; Rao NP; Crooks GM; Schiestl RH; Kasahara N. 2012. Combined preconditioning and in vivo chemoselection with 6-thioguanine alone achieves highly efficient reconstitution of normal hematopoiesis with HPRT-deficient bone marrow. Exp Hematol 40(1):3-13.e3. [PubMed: 22001673]  [MGI Ref ID J:191414]

Heckroth JA. 1994. A quantitative morphological analysis of the cerebellar nuclei in normal and lurcher mutant mice. II. Volumetric changes in cytological components. J Comp Neurol 343(1):183-92. [PubMed: 8027435]  [MGI Ref ID J:17783]

Jinnah HA; Gage FH; Friedmann T. 1991. Amphetamine-induced behavioral phenotype in a hypoxanthine-guanine phosphoribosyltransferase-deficient mouse model of Lesch-Nyhan syndrome. Behav Neurosci 105(6):1004-12. [PubMed: 1777100]  [MGI Ref ID J:1847]

Jinnah HA; Gage FH; Friedmann T. 1990. Animal models of Lesch-Nyhan syndrome. Brain Res Bull 25(3):467-75. [PubMed: 2292045]  [MGI Ref ID J:2057]

Jinnah HA; Hess EJ; Wilson MC; Gage FH; Friedmann T. 1992. Localization of hypoxanthine-guanine phosphoribosyltransferase messenger RNA in the mouse brain by insitu hybridization Mol Cell Neurosci 3(1):64-78.  [MGI Ref ID J:2058]

Jinnah HA; Jones MD; Wojcik BE; Rothstein JD; Hess EJ; Friedmann T; Breese GR. 1999. Influence of age and strain on striatal dopamine loss in a genetic mouse model of Lesch-Nyhan disease. J Neurochem 72(1):225-9. [PubMed: 9886073]  [MGI Ref ID J:51651]

Jinnah HA; Page T; Friedmann T. 1993. Brain purines in a genetic mouse model of Lesch-Nyhan disease. J Neurochem 60(6):2036-45. [PubMed: 8492116]  [MGI Ref ID J:11842]

Kamiyama H; Rauenzahn S; Shim JS; Karikari CA; Feldmann G; Hua L; Kamiyama M; Schuler FW; Lin MT; Beaty RM; Karanam B; Liang H; Mullendore ME; Mo G; Hidalgo M; Jaffee E; Hruban RH; Jinnah HA; Roden RB; Jimeno A; Liu JO; Maitra A; Eshleman JR. 2013. Personalized chemotherapy profiling using cancer cell lines from selectable mice. Clin Cancer Res 19(5):1139-46. [PubMed: 23340293]  [MGI Ref ID J:202087]

Keebaugh AC; Mitchell HA; Gaval-Cruz M; Freeman KG; Edwards GL; Weinshenker D; Thomas JW. 2011. PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse. PLoS One 6(7):e22381. [PubMed: 21818316]  [MGI Ref ID J:175856]

Kuehn MR; Bradley A; Robertson EJ; Evans MJ. 1987. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326(6110):295-8. [PubMed: 3029599]  [MGI Ref ID J:15485]

Mikolaenko I; Rao LM; Roberts RC; Kolb B; Jinnah HA. 2005. A Golgi study of neuronal architecture in a genetic mouse model for Lesch-Nyhan disease. Neurobiol Dis 20(2):479-90. [PubMed: 15908225]  [MGI Ref ID J:102545]

Moore TF; Whittingham DG. 1992. Imprinting of phosphoribosyltransferases during preimplantation development of the mouse mutant, Hprtb-m3. Development 115(4):1011-6. [PubMed: 1451655]  [MGI Ref ID J:2389]

Ordway JM; Tallaksen-Greene S; Gutekunst CA; Bernstein EM; Cearley JA; Wiener HW; Dure LS 4th; Lindsey R; Hersch SM; Jope RS; Albin RL; Detloff PJ. 1997. Ectopically expressed CAG repeats cause intranuclear inclusions and a progressive late onset neurological phenotype in the mouse. Cell 91(6):753-63. [PubMed: 9413985]  [MGI Ref ID J:44728]

Thompson S; Clarke AR; Pow AM; Hooper ML; Melton DW. 1989. Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Cell 56(2):313-21. [PubMed: 2912572]  [MGI Ref ID J:161048]

Tsuda H; Maynard-Currie CE; Reid LH; Yoshida T; Edamura K; Maeda N ; Smithies O ; Jakobovits A. 1997. Inactivation of the mouse HPRT locus by a 203-bp retroposon insertion and a 55-kb gene-targeted deletion: establishment of new HPRT-deficient mouse embryonic stem cell lines. Genomics 42(3):413-21. [PubMed: 9205113]  [MGI Ref ID J:41459]

Tucker KL; Wang Y; Dausman J; Jaenisch R. 1997. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res 25(18):3745-6. [PubMed: 9278500]  [MGI Ref ID J:92995]

Visser JE; Smith DW; Moy SS; Breese GR; Friedmann T; Rothstein JD; Jinnah HA. 2002. Oxidative stress and dopamine deficiency in a genetic mouse model of Lesch--Nyhan disease. Brain Res Dev Brain Res 133(2):127-39. [PubMed: 11882343]  [MGI Ref ID J:75479]

Wattanachanya L; Lu WD; Kundu RK; Wang L; Abbott MJ; O'Carroll D; Quertermous T; Nissenson RA. 2013. Increased bone mass in mice lacking the adipokine apelin. Endocrinology 154(6):2069-80. [PubMed: 23584856]  [MGI Ref ID J:198465]

Williamson DJ; Hooper ML; Melton DW. 1992. Mouse models of hypoxanthine phosphoribosyltransferase deficiency. J Inherit Metab Dis 15(4):665-73. [PubMed: 1528024]  [MGI Ref ID J:3354]

Wu CL; Melton DW. 1993. Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet 3(3):235-40. [PubMed: 8485579]  [MGI Ref ID J:4123]

Yang GS; Banks KG; Bonaguro RJ; Wilson G; Dreolini L; de Leeuw CN; Liu L; Swanson DJ; Goldowitz D; Holt RA; Simpson EM. 2009. Next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the Hprt1 locus. Genomics 93(3):196-204. [PubMed: 18950699]  [MGI Ref ID J:144244]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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