Description

Strain Information

Former Names B6.129P2-Hprtb-m3/J    (Changed: 15-DEC-04 ) Type Chemically Induced Mutation; Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6J Donor Strain 129P2 via E14TG2a ES cell line   Donating Investigator Ted Friedman,   UCSD School of Medicine

Appearance
black
Related Genotype: a/a

Description
HPRT-embryonic stem cells were obtained by selecting for spontaneous mutation by incubation in medium containing 6-thioguanine. HPRT-males have no overt phenotype of abnormal behavior. The mutation is due to a large deletion in the Hprt gene. Mutant mice have no HPRT detectable by Western blot analysis and no detectable HPRT enzyme activity in brain homogenates. They appear to have normal brain purine content, but de novo purine synthesis is accelerated four- to fivefold. The Hprt^b-m3 mutation has been used in preimplantation studies to determine when the maternal and paternal alleles of Hprt are activated during early embryonic development. Either administration of amphetamine or inhibition of adenine phosphoribosyltransferase (APRT) activity stimulates locomotor and stereotypic behaviors in HPRT-deficient mice. However, the null mutant for both Hprt and Aprt does not show the characteristics of Lesch-Nyhan disease.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Hprt1

004302	129S1-Hprt1tm1(cre)Mnn/J
002027	129S8/SvEv-Gpi1c Hprt1b-m2/J
003138	B6;129-Hprt1tm1Detl/J
000807	RBJ/DnJ

View Strains carrying other alleles of Hprt1     (4 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Lesch-Nyhan Syndrome; LNS - Models with phenotypic similarity to human disease where etiologies involve orthologs.1

1 Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Hprt1^b-m3/Y

        B6.129P2-Hprt1^b-m3

• nervous system phenotype
• abnormal dopaminergic neuron morphology (MGI Ref ID J:107966)
  • dopaminergic neurons from E14 Hprt-deficient mice differentiate in culture, but dendrite outgrowth is decelerated and the average dendrite length per neuron is lower than in control neurons; the difference in length increased over time in culture with the deficit being 15% after 2 days in culture to 32% at day 8

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Hprt1^b-m3/Y

        involves: 129P2/OlaHsd

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:15483)
• behavior/neurological phenotype
• abnormal sleep pattern (MGI Ref ID J:3354)
  • mice spend less time sleeping than wild-type mice
• increased grooming behavior (MGI Ref ID J:3354)
  • mice exhibit increased grooming time and trauma to the ears and flanks compared to in wild-type mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Hprt1^b-m3 related

Metabolism Research

Neurobiology Research
Neurodegeneration

Genes & Alleles

Gene & Allele Information

Allele Symbol		Hprt1b-m3
Allele Name		hypoxanthine guanine phosphoribosyl transferase B, mutation 3
Allele Type		Spontaneous
Mutation Made By		Ted Friedman,   UCSD School of Medicine
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Hprt1, hypoxanthine guanine phosphoribosyl transferase 1
Chromosome		X
Gene Common Name(s)		C81579; HGPRT; HPGRT; HPRT; Hgprtase; MGC112554; expressed sequence C81579;
General Note		HPRT- embryonic stem cells were obtained by selecting for spontaneous mutation by incubation in medium containing 6-thioguanine. HPRT- males have no overt phenotype of abnormal behavior (J:15483). The mutation is due to a large deletion in the Hprt1 gene. In situ hybridization studies showed HPRT mRNA in high levels in most neurons, but not in glial cells, in normal mice. No HPRT mRNA was detected in the brains of male mice carrying this deletion (J:2058). Mutant mice have no HPRT detectable by Western blot analysis and no detectable HPRT enzyme activity in brain homogenates. They appear to have normal brain purine content, but de novo purine synthesis is accelerated four- to fivefold (J:11842). The Hprt1b-m3 mutation has been used in preimplantation studies to determine when the maternal and paternal alleles of Hprt1 are activated during early embryonic development (J:2389). Either administration of amphetamine (J:1847) or inhibition of adenine phosphoribosyltransferase (APRT) activity (J:4123) stimulates locomotor and stereotypic behaviors in HPRT-deficient mice. However, the null mutant for both Hprt1 and Aprt does not show the characteristics of Lesch-Nyhan disease (J:35822).Cells from mice hemizygous or homozygous for this mutation are HPRT-deficient and resistant to the drug 6-thioguanine (6TG).
Molecular Note		The allele carries a 55 kb deletion spanning the promoter and first 2 exons of the gene. [MGI Ref ID J:41459]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Hooper M; Hardy K; Handyside A; Hunter S; Monk M. 1987. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature 326(6110):292-5. [PubMed: 3821905]  [MGI Ref ID J:15483]

Additional References

Gu JJ; Tolin AK; Jain J; Huang H; Santiago L; Mitchell BS. 2003. Targeted disruption of the inosine 5'-monophosphate dehydrogenase type I gene in mice. Mol Cell Biol 23(18):6702-12. [PubMed: 12944494]  [MGI Ref ID J:85443]

Jinnah HA; Gage FH; Friedmann T. 1991. Amphetamine-induced behavioral phenotype in a hypoxanthine-guanine phosphoribosyltransferase-deficient mouse model of Lesch-Nyhan syndrome. Behav Neurosci 105(6):1004-12. [PubMed: 1777100]  [MGI Ref ID J:1847]

Jinnah HA; Page T; Friedmann T. 1993. Brain purines in a genetic mouse model of Lesch-Nyhan disease. J Neurochem 60(6):2036-45. [PubMed: 8492116]  [MGI Ref ID J:11842]

Moore TF; Whittingham DG. 1992. Imprinting of phosphoribosyltransferases during preimplantation development of the mouse mutant, Hprtb-m3. Development 115(4):1011-6. [PubMed: 1451655]  [MGI Ref ID J:2389]

Wu CL; Melton DW. 1993. Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet 3(3):235-40. [PubMed: 8485579]  [MGI Ref ID J:4123]

Hprt1^b-m3 related

Boer P; Brosh S; Wasserman L; Hammel I; Zoref-Shani E; Sperling O. 2001. Decelerated rate of dendrite outgrowth from dopaminergic neurons in primary cultures from brains of hypoxanthine phosphoribosyltransferase-deficient knockout mice. Neurosci Lett 303(1):45-8. [PubMed: 11297820]  [MGI Ref ID J:107966]

Egami K; Yitta S; Kasim S; Lewers JC; Roberts RC; Lehar M; Jinnah HA. 2007. Basal ganglia dopamine loss due to defect in purine recycling. Neurobiol Dis 26(2):396-407. [PubMed: 17374562]  [MGI Ref ID J:134847]

Engle SJ; Womer DE; Davies PM; Boivin G; Sahota A; Simmonds HA; Stambrook PJ; Tischfield JA. 1996. HPRT-APRT-deficient mice are not a model for lesch-nyhan syndrome. Hum Mol Genet 5(10):1607-10. [PubMed: 8894695]  [MGI Ref ID J:35822]

Gu JJ; Stegmann S; Gathy K; Murray R; Laliberte J; Ayscue L; Mitchell BS. 2000. Inhibition of T lymphocyte activation in mice heterozygous for loss of the IMPDH II gene. J Clin Invest 106(4):599-606. [PubMed: 10953035]  [MGI Ref ID J:86045]

Gu JJ; Tolin AK; Jain J; Huang H; Santiago L; Mitchell BS. 2003. Targeted disruption of the inosine 5'-monophosphate dehydrogenase type I gene in mice. Mol Cell Biol 23(18):6702-12. [PubMed: 12944494]  [MGI Ref ID J:85443]

Jinnah HA; Gage FH; Friedmann T. 1991. Amphetamine-induced behavioral phenotype in a hypoxanthine-guanine phosphoribosyltransferase-deficient mouse model of Lesch-Nyhan syndrome. Behav Neurosci 105(6):1004-12. [PubMed: 1777100]  [MGI Ref ID J:1847]

Jinnah HA; Hess EJ; Wilson MC; Gage FH; Friedmann T. 1992. Localization of hypoxanthine-guanine phosphoribosyltransferase messenger RNA in the mouse brain by insitu hybridization Mol Cell Neurosci 3(1):64-78.  [MGI Ref ID J:2058]

Jinnah HA; Jones MD; Wojcik BE; Rothstein JD; Hess EJ; Friedmann T; Breese GR. 1999. Influence of age and strain on striatal dopamine loss in a genetic mouse model of Lesch-Nyhan disease. J Neurochem 72(1):225-9. [PubMed: 9886073]  [MGI Ref ID J:51651]

Jinnah HA; Page T; Friedmann T. 1993. Brain purines in a genetic mouse model of Lesch-Nyhan disease. J Neurochem 60(6):2036-45. [PubMed: 8492116]  [MGI Ref ID J:11842]

Mikolaenko I; Rao LM; Roberts RC; Kolb B; Jinnah HA. 2005. A Golgi study of neuronal architecture in a genetic mouse model for Lesch-Nyhan disease. Neurobiol Dis 20(2):479-90. [PubMed: 15908225]  [MGI Ref ID J:102545]

Moore TF; Whittingham DG. 1992. Imprinting of phosphoribosyltransferases during preimplantation development of the mouse mutant, Hprtb-m3. Development 115(4):1011-6. [PubMed: 1451655]  [MGI Ref ID J:2389]

Ordway JM; Tallaksen-Greene S; Gutekunst CA; Bernstein EM; Cearley JA; Wiener HW; Dure LS 4th; Lindsey R; Hersch SM; Jope RS; Albin RL; Detloff PJ. 1997. Ectopically expressed CAG repeats cause intranuclear inclusions and a progressive late onset neurological phenotype in the mouse. Cell 91(6):753-63. [PubMed: 9413985]  [MGI Ref ID J:44728]

Tsuda H; Maynard-Currie CE; Reid LH; Yoshida T; Edamura K; Maeda N ; Smithies O ; Jakobovits A. 1997. Inactivation of the mouse HPRT locus by a 203-bp retroposon insertion and a 55-kb gene-targeted deletion: establishment of new HPRT-deficient mouse embryonic stem cell lines. Genomics 42(3):413-21. [PubMed: 9205113]  [MGI Ref ID J:41459]

Tucker KL; Wang Y; Dausman J; Jaenisch R. 1997. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res 25(18):3745-6. [PubMed: 9278500]  [MGI Ref ID J:92995]

Visser JE; Smith DW; Moy SS; Breese GR; Friedmann T; Rothstein JD; Jinnah HA. 2002. Oxidative stress and dopamine deficiency in a genetic mouse model of Lesch--Nyhan disease. Brain Res Dev Brain Res 133(2):127-39. [PubMed: 11882343]  [MGI Ref ID J:75479]

Williamson DJ; Hooper ML; Melton DW. 1992. Mouse models of hypoxanthine phosphoribosyltransferase deficiency. J Inherit Metab Dis 15(4):665-73. [PubMed: 1528024]  [MGI Ref ID J:3354]

Wu CL; Melton DW. 1993. Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet 3(3):235-40. [PubMed: 8485579]  [MGI Ref ID J:4123]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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