Strain Name:

STOCK Tg(B19-RNAi:Il3)241Ckn/J

Stock Number:

002182

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Tg(B19-RNAi:IL3)241Ckn/J    (Changed: 01-FEB-07 )
FVB.KIV-ASIL3/(241N)J    (Changed: 22-JAN-07 )
FVB.KIV-ASIL3 (241N)    (Changed: 15-DEC-04 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse

Development
The transgenic construct consists of a 990 bp fragment corresponding to the full length antisense murine Il3 cDNA under the control of the B19 parvovirus promoter and a 3’ T cell dominant control region from the 3’ end of the human CD2 gene. The construct was injected into fertilized eggs from FVB/N female mice mated to CB6F1 males in the laboratory of Dr. Cynthia Dunbar at the NIH. Founder line 241 exhibits the highest penetrance of the two phenotypes. This transgenic was crossed to a strain designated KIV to generate heterozygosity at the Hprt locus. (Unfortunately, we do not have information regarding the origins or characteristics of strain KIV.) The strain was then backcrossed to FVB/N for three generations. The donating investigator reports that these transgenic mice manifest a phenotype identical to the strain reported in Cockayne DA et al. Blood 84:2699 (1994). In 1993, The Jackson Laboratory imported this line and mated transgenic males to FVB/NMob females for an additional backcross. The line was cryopreserved in 1994.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(B19-RNAi:Il3)241Ckn/?

        involves: BALB/cJ * C57BL/6J * FVB/N
  • mortality/aging
  • premature death
    • incomplete penetrance of phenotypes results in some mice developing either a fatal neurological disorder or fatal pre-B cell lymphproliferative syndrome by 3-6 months of age   (MGI Ref ID J:107618)
  • immune system phenotype
  • abnormal B cell differentiation
    • evidence of lymphoproliferative syndrome is observed by 3 months of age in spleen and lymph nodes   (MGI Ref ID J:107618)
    • lymphoproliferation is characterized by cellular infiltrate populated primarily by pre-B cells (B220+/sIgM-)   (MGI Ref ID J:107618)
    • bone marrow, thymus, spinal cord and skeletal muscle fibers develop cellular infiltrate late in the disease process   (MGI Ref ID J:107618)
    • increased pre-B cell number
      • 90% of cells in lymph node express B220+/sIgM- surface antigens, however, absolute number of peripheral T cells is unchanged   (MGI Ref ID J:107618)
  • decreased interleukin-3 secretion
    • ConA-stimulated splenocytes from asymptomatic transgenic mice produce significantly less IL3 than stimulated controls   (MGI Ref ID J:107618)
  • enlarged lymph nodes
    • peripheral adenopathy   (MGI Ref ID J:107618)
    • observed in mice with lymphoproliferative syndrome   (MGI Ref ID J:107618)
    • contain cellular infiltrate of immature-appearing, mitotically active round cells   (MGI Ref ID J:107618)
  • enlarged spleen
    • observed in mice with lymphoproliferative syndrome   (MGI Ref ID J:107618)
    • contains cellular infiltrate of immature-appearing, mitotically active round cells   (MGI Ref ID J:107618)
  • behavior/neurological phenotype
  • ataxia
    • observed in mice with neurological disorder between 3-6 months of age   (MGI Ref ID J:107618)
  • circling
    • first observed in mice with neurological disorder between 3-6 months of age, progressing to ataxia, then wasting within 2-4 weeks   (MGI Ref ID J:107618)
  • hindlimb paralysis
    • occurs in some mice in late stages of lymphoproliferative syndrome   (MGI Ref ID J:107618)
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • evidence of lymphoproliferative syndrome is observed by 3 months of age in spleen and lymph nodes   (MGI Ref ID J:107618)
    • lymphoproliferation is characterized by cellular infiltrate populated primarily by pre-B cells (B220+/sIgM-)   (MGI Ref ID J:107618)
    • bone marrow, thymus, spinal cord and skeletal muscle fibers develop cellular infiltrate late in the disease process   (MGI Ref ID J:107618)
    • increased pre-B cell number
      • 90% of cells in lymph node express B220+/sIgM- surface antigens, however, absolute number of peripheral T cells is unchanged   (MGI Ref ID J:107618)
  • enlarged spleen
    • observed in mice with lymphoproliferative syndrome   (MGI Ref ID J:107618)
    • contains cellular infiltrate of immature-appearing, mitotically active round cells   (MGI Ref ID J:107618)
  • growth/size/body phenotype
  • cachexia
    • observed in mice with neurological disorder   (MGI Ref ID J:107618)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tg(B19-RNAi:Il3)241Ckn related

Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects

Neurobiology Research
Ataxia (Movement) Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(B19-RNAi:Il3)241Ckn
Allele Name transgene insertion 241, Debra A Cockayne
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) AS-IL-3;
Strain of OriginFVB/N X (BALB/c x C57BL/6)F1
Expressed Gene Il3, antisense RNA,
Promoter B19, B19 parvovirus, virus
General Note Of 5 transgenic founder lines, two exhibited no abnormal phenotype and were not further characterized. One of the other founder mice died of neurologic disease at 3 months, without having bred. Founders 241 and 244 transmitted the transgene and both the lymphoproliferative and neurologic phenotypes to their progeny. The pathology of the neurologic and of the lymphoproliferative disorders differs between the two lines only in penetrance and in age of onset (3-6 months for line 241 compared to 6-12 months for line 244).
Molecular Note This transgene consists of the full length, 990-bp mouse interleukin 3 cDNA, in reverse (antisense) orientation, under control of the B19 parvovirus promoter and a T-cell dominant control region from the 3' end of the human CD2 gene. A human beta-globin gene segment including the 3' end of exon 2, all of intron 2 and exon 3, the polyadenylation signal and some 3' flanking DNA is present just downstream of the Il3 sequence. Interleukin 3 antisense RNA is detected by RT-PCR analysis in mature CD3+ T lymphocytes of transgenic animals both with lymphoproliferation and with no disease phenotype, in transgenic B220+IgM- pre-B cells, and in brains of both asymptomatic and neurologically affected transgenic mice. Il3 antisense transcripts are present in cultured splenocytes from asymptomatic transgenic mice following in vitro concanavalin A (ConA) stimulation, and ELISA of supernatants from these cultures demonstrates a 5-fold reduction in interleukin 3 secretion compared to similar cultures of control splenocytes. [MGI Ref ID J:107618]
 
 

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cockayne DA; Bodine DM; Cline A; Nienhuis AW; Dunbar CE. 1994. Transgenic mice expressing antisense interleukin-3 RNA develop a B-cell lymphoproliferative syndrome or neurologic dysfunction. Blood 84(8):2699-710. [PubMed: 7919383]  [MGI Ref ID J:107618]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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