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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6J-TgN(MTnlacZ)204Bri (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+6F12p
Generation DefinitionsDonating Investigator Dr. Ralph Brinster, University of Pennsylvania Appearance
black
Related Genotype: a/aDescription
These lacZ transgenic mice carry no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes from this strain stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions.Development
The mouse Mt1 (metallothionein) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of the vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites that appear to be important for enhanced expression.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
002211 129S7/SvEvBrd-Mt1tm1Bri Mt2tm1Bri/J 017833 B6.Cg-Tg(BCR/ABL)623Hkp/J 002210 B6.Cg-Tg(Mt1)174Bri/J 008850 B6;SJL-Tg(Mt1-LDLR)93-4Reh/AgnJ 003210 C57BL/6-Tg(Crh)227.1Pbl/J 002599 C57BL/6-Tg(ML5sHEL)5Ccg/J 005967 C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn 005968 C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn/Pgn 002028 D2.Cg-Tg(pHRD)1Ust/J 002421 FVB/N-Tg(MtTGFA)100Lmb/J 002675 FVB/N-Tg(MtTPRMET)243Lng/J 002775 FVB/N-Tg(MtTPRMET)773Lng/J 006610 NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ 002209 STOCK Tg(Mt1)174Bri/J 002422 STOCK Tg(MtTGFA)42Lmb/J 003722 SW.Cg-Tg(MtTGFA)42Lmb/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: inducible hepatic cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(MTn-lacZ)204Bri | ||
|---|---|---|---|
| Allele Name | transgene insertion 204, Ralph L Brinster | ||
| Allele Type | Transgenic (Reporter) | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | lacZ is expressed in hepatocytes. Protein product is localized to cell nucleus by SV40 large T-antigen nuclear localization signal peptide. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | Mt1, metallothionein 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(MTn-lacZ)204Bri, transgene insertion 204, Ralph L Brinster | ||
| Chromosome | UN | ||
| General Note | Transgenic mice on a C57BL/6J background display no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions. | ||
| Molecular Note | The mouse Mt1 (metallothionein 1) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of a vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites thought to be important for enhanced expression. [MGI Ref ID J:92451] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Rhim JA; Sandgren EP; Degen JL; Palmiter RD; Brinster RL. 1994. Replacement of diseased mouse liver by hepatic cell transplantation. Science 263(5150):1149-52. [PubMed: 8108734] [MGI Ref ID J:92451]
Additional References
Tg(MTn-lacZ)204Bri relatedKanatsu-Shinohara M; Morimoto T; Toyokuni S; Shinohara T. 2004. Regulation of mouse spermatogonial stem cell self-renewing division by the pituitary gland. Biol Reprod 70(6):1731-7. [PubMed: 14766726] [MGI Ref ID J:132826]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This colony is maintained by homozygous sibling matings. Expected coat color from breeding:Black Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3000.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
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General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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