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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names C57BL/6J-TgN(MTnlacZ)204Bri (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N?+6F12p Donating Investigator Ralph Brinster, University of Pennsylvania Appearance
black
Related Genotype: a/aDescription
These lacZ transgenic mice carry no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes from this strain stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions.Development
The mouse Mt1 (metallothionein) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of the vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites that appear to be important for enhanced expression.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (178 strains)
002211 129S7/SvEvBrd-Mt1tm1Bri Mt2tm1Bri/J 002210 B6.Cg-Tg(Mt1)174Bri/J 003210 C57BL/6-Tg(Crh)227.1Pbl/J 002599 C57BL/6-Tg(ML5sHEL)5Ccg/J 005968 C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn/Pgn 002028 D2.Cg-Tg(pHRD)1Ust/J 002421 FVB/N-Tg(MtTGFA)100Lmb/J 002675 FVB/N-Tg(MtTPRMET)243Lng/J 002775 FVB/N-Tg(MtTPRMET)773Lng/J 004252 NOD.B6-Tg(ML5sHEL)5Ccg/Dvs 006610 NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ 002209 STOCK Tg(Mt1)174Bri/J 002422 STOCK Tg(MtTGFA)42Lmb/J 003722 SW.Cg-Tg(MtTGFA)42Lmb/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Mt1 relatedResearch Tools
lacZ Expression
Genetics Research (Tissue/Cell Markers: inducible hepatic cell marker)
lacZ relatedMetabolism Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(MTn-lacZ)204Bri | ||
|---|---|---|---|
| Allele Name | transgene insertion 204, Ralph L Brinster | ||
| Allele Type | Transgenic (Reporter) | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | lacZ is expressed in hepatocytes. Protein product is localized to cell nucleus by SV40 large T-antigen nuclear localization signal peptide. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | Mt1, metallothionein 1, mouse, laboratory | ||
| General Note | Transgenic mice on a C57BL/6J background display no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions. | ||
| Molecular Note | The mouse Mt1 (metallothionein 1) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of a vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites thought to be important for enhanced expression. [MGI Ref ID J:92451] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Rhim JA; Sandgren EP; Degen JL; Palmiter RD; Brinster RL. 1994. Replacement of diseased mouse liver by hepatic cell transplantation. Science 263(5150):1149-52. [PubMed: 8108734] [MGI Ref ID J:92451]
Additional References
Tg(MTn-lacZ)204Bri relatedKanatsu-Shinohara M; Morimoto T; Toyokuni S; Shinohara T. 2004. Regulation of mouse spermatogonial stem cell self-renewing division by the pituitary gland. Biol Reprod 70(6):1731-7. [PubMed: 14766726] [MGI Ref ID J:132826]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This colony is maintained by homozygous sibling matings. Expected coat color from breeding:Black Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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