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Strain Name:

B6;CBA-Tg(CD3E)26Cpt/J

Stock Number:

002194

Availability:

Repository-Cryopreserved

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Genes & Alleles   CD3E;   Rag1;   Tg(CD3E)26Cpt;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Cox Terhorst,   Beth Israel Deaconess Medical Center
GenerationF?+2

Appearance
agouti
Related Genotype: A/A

Strain Description
Homozygous mice carrying the (CD3E)26Cpt transgene lack mature T lymphocytes and NK cells. The thymus is especially small.

Mammalian Phenotype Terms assigned by genotype

Tg(CD3E)26Cpt/Tg(CD3E)26Cpt

        involves: C57BL/6 * CBA
  • life span-post-weaning/aging
  • decreased survivor rate (MGI Ref ID J:134589)
    • only 40% of mice survive until weaning
  • lethality-prenatal/perinatal
  • neonatal lethality (MGI Ref ID J:134589)
    • 11% of mice die within 24 hours of birth
  • lethality-postnatal
  • postnatal lethality (MGI Ref ID J:134589)
    • 29% of mice die before weaning
  • growth/size phenotype
  • decreased body weight (MGI Ref ID J:134589)
    • mice weigh 29% less than age- and sex- matched controls at three weeks of age and 27% less than controls at 7 weeks of age
  • immune system phenotype
  • abnormal thymus medulla morphology (MGI Ref ID J:81711)
    • no clear distinction exists between the cortex and the medulla
  • absent NK cells (MGI Ref ID J:81711)
    • NK cells are absent in these mice based upon the lack of splenocytes expressing LGL-1, NK1.1, and "bright" levels of AGM-1
    • the NK target cell line YAC-1 is not lysed when co-cultured with activated splenocytes also indicating an absence of NK cells
  • arrested T cell differentiation (MGI Ref ID J:81711)
    • very few thymocytes are present with the vast majority of them arrested in the double negative stage
  • decreased thymocyte number (MGI Ref ID J:81711)
    • mean thymocyte number in mice up to 6 months of age is decreased 70-fold compared to controls
  • decreased uterine NK cell number (MGI Ref ID J:134589)
    • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls
    • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta
  • reproductive system phenotype
  • abnormal uterus morphology (MGI Ref ID J:134589)
    • the uterine arterioles undergo abnormal histological changes during the pregnancy of homozygote transgenic females mated with homozygotic transgenic males
    • at 10 days of gestation, the endothelial cells become cuboidal with thickening of the arteriolar media and adventitia occurring
    • at 12 days of gestation, the endothelium becomes flat and deposition of lipid and foam cells occurs
    • at 14 days of gestation, loss of vessel integrity occurs with a discontinous endothelium and leakage of blood
    • similar histological changes are observed during the first 12 days of gestation that result from breeding homozygote transgenic females with wild-type CD-1 males
    • abnormal endometrium morphology (MGI Ref ID J:134589)
      • the metrial gland and uterine NK cells are absent from the uteri of pregnant mice at day 14 of gestation
  • decreased litter size (MGI Ref ID J:134589)
    • matings of homozygous mice produce on average 6 pups while control mice produce 9 pus
  • reduced fertility (MGI Ref ID J:134589)
    • fetal viability is low with 67% of embryos being in the late stages of resorption at E14
    • the loss of fetuses starts at 10 days post-conception
  • embryogenesis phenotype
  • abnormal placenta morphology (MGI Ref ID J:134589)
    • placenta morphology is abnormal in homozygote transgenic females mated with wild-type CD-1 males
    • at 12 days of gestation, the placenta becomes more disorganized with variation in appearances between implantation sites and trophoblast cells moving adjacent to decidual artioles
    • abnormal placental labyrinth morphology (MGI Ref ID J:134589)
      • placenta labyrinth morphology is abnormal in homozygote transgenic females mated with wild-type CD-1 males
      • extremely large syncytia are present with peripheral nuclei surrounding a vacuolated cytoplasm
      • extensive smooth muscle development is sometimes found adjacent to the labyrinthine trophoblast
    • small placenta (MGI Ref ID J:134589)
      • the placenta of mice 14 days after gestation is 45% the size of control CBA mice
      • reductions of placenta size are evident at 10 days of gestation
  • hematopoietic system phenotype
  • abnormal thymus medulla morphology (MGI Ref ID J:81711)
    • no clear distinction exists between the cortex and the medulla
  • absent NK cells (MGI Ref ID J:81711)
    • NK cells are absent in these mice based upon the lack of splenocytes expressing LGL-1, NK1.1, and "bright" levels of AGM-1
    • the NK target cell line YAC-1 is not lysed when co-cultured with activated splenocytes also indicating an absence of NK cells
  • arrested T cell differentiation (MGI Ref ID J:81711)
    • very few thymocytes are present with the vast majority of them arrested in the double negative stage
  • decreased thymocyte number (MGI Ref ID J:81711)
    • mean thymocyte number in mice up to 6 months of age is decreased 70-fold compared to controls
  • decreased uterine NK cell number (MGI Ref ID J:134589)
    • uterine NK cells in pregnant mice at 14 days of gestation are only found at 3% the number found in CBA controls
    • the uterine NK cells are restricted in their location to the spongiotrophoblast region of the placenta

Gene & Allele Details

Allele Symbol Tg(CD3E)26Cpt
Allele Name transgene insertion 26, Cox Terhorst
Common Name(s) CD3epsilon; Tgepsilon26; tgE26;
Mutation Made By Cox Terhorst,   Beth Israel Deaconess Medical Center
Strain of Origin(C57BL/6J x CBA/J)F2
Expressed Gene CD3E, CD3e molecule, epsilon (CD3-TCR complex), human
Promoter Rag1, recombination activating gene 1, mouse, laboratory
Molecular Note The transgene contains CD3 antigen, epsilon polypeptide (Human) sequences. 30-35 copies of the transgene integrated into the genome. [MGI Ref ID J:81711]

Control Information

  Control
   100011 B6CBAF1/J (approximate)
   000664 C57BL/6J (approximate)
   000656 CBA/J (approximate)
   There are no appropriate physiological controls for this mutant strain. However, if you must have a control you may use any of the following strains: B6CBAF1/J (Stock No. 100011), C57BL/6J (Stock No. 000664), and CBA/J (Stock No. 000656). These strains also serve as DNA controls.
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(CD3E)26Cpt

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Tg(CD3E)26Cpt allele
002506   STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
View Strains carrying   Tg(CD3E)26Cpt     (1 strain)

View Strains carrying other alleles of Rag1     (17 strains)

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Immunology and Inflammation Research (B and T cell deficiency)
Immunology and Inflammation Research (NK Cell Deficiency)

CD3E related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Immunology and Inflammation Research (T cell deficiency)

References

Selected Reference(s)

Wang B; Biron C; She J; Higgins K; Sunshine MJ; Lacy E; Lonberg N; Terhorst C. 1994. A block in both early T lymphocyte and natural killer cell development in transgenic mice with high-copy numbers of the human CD3E gene. Proc Natl Acad Sci U S A 91(20):9402-6. [PubMed: 7937778]  [MGI Ref ID J:81711]

Additional References

Price and Supply Information

Strain Name: B6;CBA-Tg(CD3E)26Cpt/J
Stock Number: 002194

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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