Strain Name:

B6.Cg-Tg(Mt1)174Bri/J

Stock Number:

002210

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names C57BL/6J-Tg(Mt1)174Bri/J    (Changed: 05-APR-07 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Development
The "Tg(Mt-1)Bri 174" transgene was designed with a mouse metallothionein 1 gene (modified with an additional 2 nucleotides located 9 nucleotides upstream of the translational initiation codon) flanked by 10kb of 5' and 7kb of 3' regulatory sequences. The construct was injected into fertilized eggs from (C57BL/6 x SJL)F1 female and male origin. Mutant mice were maintained by crossing heterozygous carriers to B6SJLF1 animals. Subsequently B6SJL heterozygotes were mated to CD-1 (outbred strain) and carriers from this mating were bred to produce homozygotes. The resulting strain (on a mixed C57BL/6, SJL, and CD-1 genetic background - see Stock No. 002209) was backcrossed at least 5 generations to C57BL/6J after arrival at The Jackson Laboratory to generate this congenic strain (Stock No. 002210).

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Mt1)174Bri allele
002209   STOCK Tg(Mt1)174Bri/J
View Strains carrying   Tg(Mt1)174Bri     (1 strain)

View Strains carrying other alleles of Mt1     (13 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(Mt1)174Bri/0

        involves: C57BL/6 * SJL
  • homeostasis/metabolism phenotype
  • abnormal mineral level (MGI Ref ID J:29102)
    • mice have 50% more zinc in the liver and 3-fold more zinc in the pancreas than is found in non-transgenic littermate controls

Tg(Mt1)174Bri/Tg(Mt1)174Bri

        involves: C57BL/6 * SJL
  • digestive/alimentary phenotype
  • abnormal mineral absorption (MGI Ref ID J:47419)
    • 2 hours after oral dosing with zinc, mice have about a 2-fold less zinc concentration in the blood than in control mice
  • homeostasis/metabolism phenotype
  • altered response to myocardial infarction (MGI Ref ID J:85798)
    • the mean percentage of apoptotic cells present in the myocardium after ischemia/reperfusion is 14.4% compared to 26.3%
    • myocardial oxidative damage is reduced by almost two-thirds as measured by levels of lipid peroxidation found in the heart after ischemia/reperfusion
    • decreased infarction size (MGI Ref ID J:85798)
      • only small scattered infarct zones are present after ischemia/reperfusion-induced myocardial infarction
      • area of tissue with infarctions visible is only about half that of non-transgenic controls
  • cardiovascular system phenotype
  • altered response to myocardial infarction (MGI Ref ID J:85798)
    • the mean percentage of apoptotic cells present in the myocardium after ischemia/reperfusion is 14.4% compared to 26.3%
    • myocardial oxidative damage is reduced by almost two-thirds as measured by levels of lipid peroxidation found in the heart after ischemia/reperfusion
    • decreased infarction size (MGI Ref ID J:85798)
      • only small scattered infarct zones are present after ischemia/reperfusion-induced myocardial infarction
      • area of tissue with infarctions visible is only about half that of non-transgenic controls
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Mt1 related

Metabolism Research

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Mt1)174Bri
Allele Name transgene insertion 174, Ralph L Brinster
Allele Type Transgenic (random, expressed)
Common Name(s) MT-TG; MTtrans; TgMTI;
Mutation Made By Ralph Brinster,   University of Pennsylvania
Strain of Origin(C57BL/6 x SJL)F2
Expressed Gene Mt1, metallothionein 1, mouse, laboratory
Molecular Note The transgene contains mouse metallothionein 1 sequence with 10kb of upstream sequence and 7kb of downstream sequence. 56 copies of the transgene integrated in this line. [MGI Ref ID J:92452]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Mt1)174Bri, STD PCR, vers. 3

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Palmiter RD; Sandgren EP; Koeller DM; Brinster RL. 1993. Distal regulatory elements from the mouse metallothionein locus stimulate gene expression in transgenic mice. Mol Cell Biol 13(9):5266-75. [PubMed: 8355681]  [MGI Ref ID J:92452]

Additional References

Kang YJ; Li Y; Sun X; Sun X. 2003. Antiapoptotic effect and inhibition of ischemia/reperfusion-induced myocardial injury in metallothionein-overexpressing transgenic mice. Am J Pathol 163(4):1579-86. [PubMed: 14507664]  [MGI Ref ID J:85798]

Waelput W; Broekaert D; Vandekerckhove J; Brouckaert P; Tavernier J; Libert C. 2001. A mediator role for metallothionein in tumor necrosis factor-induced lethal shock. J Exp Med 194(11):1617-24. [PubMed: 11733576]  [MGI Ref ID J:73099]

Tg(Mt1)174Bri related

Davis SR; McMahon RJ; Cousins RJ. 1998. Metallothionein knockout and transgenic mice exhibit altered intestinal processing of zinc with uniform zinc-dependent zinc transporter-1 expression. J Nutr 128(5):825-31. [PubMed: 9566988]  [MGI Ref ID J:47419]

Davis SR; Samuelson DA; Cousins RJ. 2001. Metallothionein expression protects against carbon tetrachloride-induced hepatotoxicity, but overexpression and dietary zinc supplementation provide no further protection in metallothionein transgenic and knockout mice. J Nutr 131(2):215-22. [PubMed: 11160536]  [MGI Ref ID J:107225]

Deng DX; Cai L; Chakrabarti S; Cherian MG. 1999. Increased radiation-induced apoptosis in mouse thymus in the absence of metallothionein. Toxicology 134(1):39-49. [PubMed: 10413187]  [MGI Ref ID J:57169]

Ebadi M; Brown-Borg H; El Refaey H; Singh BB; Garrett S; Shavali S; Sharma SK. 2005. Metallothionein-mediated neuroprotection in genetically engineered mouse models of Parkinson's disease. Brain Res Mol Brain Res 134(1):67-75. [PubMed: 15790531]  [MGI Ref ID J:97105]

Ebadi M; Sharma S. 2006. Metallothioneins 1 and 2 attenuate peroxynitrite-induced oxidative stress in Parkinson disease. Exp Biol Med (Maywood) 231(9):1576-83. [PubMed: 17018883]  [MGI Ref ID J:129277]

Giralt M; Penkowa M; Lago N; Molinero A; Hidalgo J. 2002. Metallothionein-1+2 protect the CNS after a focal brain injury. Exp Neurol 173(1):114-28. [PubMed: 11771944]  [MGI Ref ID J:119136]

Iszard MB; Liu J; Liu Y; Dalton T; Andrews GK; Palmiter RD; Klaassen CD. 1995. Characterization of metallothionein-I-transgenic mice. Toxicol Appl Pharmacol 133(2):305-12. [PubMed: 7645027]  [MGI Ref ID J:29102]

Kang YJ; Li Y; Sun X; Sun X. 2003. Antiapoptotic effect and inhibition of ischemia/reperfusion-induced myocardial injury in metallothionein-overexpressing transgenic mice. Am J Pathol 163(4):1579-86. [PubMed: 14507664]  [MGI Ref ID J:85798]

Lau JC; Cherian MG. 1998. Developmental changes in hepatic metallothionein, zinc, and copper levels in genetically altered mice. Biochem Cell Biol 76(4):615-23. [PubMed: 10099782]  [MGI Ref ID J:53230]

Lee DK; Fu K; Liang L; Dalton T; Palmiter RD; Andrews GK. 1996. Transgenic mouse blastocysts that overexpress metallothionein-I resist cadmium toxicity in vitro. Mol Reprod Dev 43(2):158-66. [PubMed: 8824913]  [MGI Ref ID J:31252]

Molinero A; Penkowa M; Hernandez J; Camats J; Giralt M; Lago N; Carrasco J; Campbell IL; Hidalgo J. 2003. Metallothionein-I overexpression decreases brain pathology in transgenic mice with astrocyte-targeted expression of interleukin-6. J Neuropathol Exp Neurol 62(3):315-28. [PubMed: 12638735]  [MGI Ref ID J:104847]

Ono S; Koropatnick DJ; Cherian MG. 1997. Regional brain distribution of metallothionein, zinc and copper in toxic milk mutant and transgenic mice. Toxicology 124(1):1-10. [PubMed: 9392450]  [MGI Ref ID J:44523]

Oz HS; Chen T; de Villiers WJ; McClain CJ. 2005. Metallothionein overexpression does not protect against inflammatory bowel disease in a murine colitis model. Med Sci Monit 11(3):BR69-73. [PubMed: 15735556]  [MGI Ref ID J:127866]

Sharma SK; El Refaey H; Ebadi M. 2006. Complex-1 activity and 18F-DOPA uptake in genetically engineered mouse model of Parkinson's disease and the neuroprotective role of coenzyme Q10. Brain Res Bull 70(1):22-32. [PubMed: 16750479]  [MGI Ref ID J:112754]

Song Y; Wang J; Li Y; Du Y; Arteel GE; Saari JT; Kang YJ; Cai L. 2005. Cardiac metallothionein synthesis in streptozotocin-induced diabetic mice, and its protection against diabetes-induced cardiac injury. Am J Pathol 167(1):17-26. [PubMed: 15972948]  [MGI Ref ID J:99513]

Waalkes MP; Liu J; Kasprzak KS; Diwan BA. 2004. Minimal influence of metallothionein over-expression on nickel carcinogenesis in mice. Toxicol Lett 153(3):357-64. [PubMed: 15454311]  [MGI Ref ID J:93373]

Waelput W; Broekaert D; Vandekerckhove J; Brouckaert P; Tavernier J; Libert C. 2001. A mediator role for metallothionein in tumor necrosis factor-induced lethal shock. J Exp Med 194(11):1617-24. [PubMed: 11733576]  [MGI Ref ID J:73099]

Wesselkamper SC; McDowell SA; Medvedovic M; Dalton TP; Deshmukh HS; Sartor MA; Case LM; Henning LN; Borchers MT; Tomlinson CR; Prows DR; Leikauf GD. 2006. The role of metallothionein in the pathogenesis of acute lung injury. Am J Respir Cell Mol Biol 34(1):73-82. [PubMed: 16166738]  [MGI Ref ID J:120196]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice can be bred. The expected coat color from breeding is black.
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


(3.2)