Strain Name:

B6.Cg-Tg(Mt1)174Bri/J

Stock Number:

002210

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C57BL/6J-Tg(Mt1)174Bri/J    (Changed: 05-APR-07 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Development
The "Tg(Mt-1)Bri 174" transgene was designed with a mouse metallothionein 1 gene (modified with an additional 2 nucleotides located 9 nucleotides upstream of the translational initiation codon) flanked by 10kb of 5' and 7kb of 3' regulatory sequences. The construct was injected into fertilized eggs from (C57BL/6 x SJL)F1 female and male origin. Mutant mice were maintained by crossing heterozygous carriers to B6SJLF1 animals. Subsequently B6SJL heterozygotes were mated to CD-1 (outbred strain) and carriers from this mating were bred to produce homozygotes. The resulting strain (on a mixed C57BL/6, SJL, and CD-1 genetic background - see Stock No. 002209) was backcrossed at least 5 generations to C57BL/6J after arrival at The Jackson Laboratory to generate this congenic strain (Stock No. 002210).

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Mt1)174Bri allele
002209   STOCK Tg(Mt1)174Bri/J
View Strains carrying   Tg(Mt1)174Bri     (1 strain)

View Strains carrying other alleles of Mt1     (15 strains)

Strains carrying other alleles of Tg(Mt1)174Bri
002209   STOCK Tg(Mt1)174Bri/J
View Strains carrying other alleles of Tg(Mt1)174Bri     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Mt1)174Bri/0

        B6.Cg-Tg(Mt1)174Bri/J
  • mortality/aging
  • decreased sensitivity to xenobiotic induced morbidity/mortality
    • treated-treated mice exhibit increased mean survival time compared with similarly treated wild-type mice   (MGI Ref ID J:120196)
  • homeostasis/metabolism phenotype
  • decreased sensitivity to xenobiotic induced morbidity/mortality
    • treated-treated mice exhibit increased mean survival time compared with similarly treated wild-type mice   (MGI Ref ID J:120196)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(Mt1)174Bri/0

        involves: C57BL/6 * SJL
  • homeostasis/metabolism phenotype
  • abnormal mineral level
    • mice have 50% more zinc in the liver and 3-fold more zinc in the pancreas than is found in non-transgenic littermate controls   (MGI Ref ID J:29102)

Tg(Mt1)174Bri/Tg(Mt1)174Bri

        involves: C57BL/6 * SJL
  • digestive/alimentary phenotype
  • abnormal intestinal mineral absorption
    • 2 hours after oral dosing with zinc, mice have about a 2-fold less zinc concentration in the blood than in control mice   (MGI Ref ID J:47419)
  • homeostasis/metabolism phenotype
  • altered response to myocardial infarction
    • the mean percentage of apoptotic cells present in the myocardium after ischemia/reperfusion is 14.4% compared to 26.3%   (MGI Ref ID J:85798)
    • myocardial oxidative damage is reduced by almost two-thirds as measured by levels of lipid peroxidation found in the heart after ischemia/reperfusion   (MGI Ref ID J:85798)
    • decreased infarction size
      • only small scattered infarct zones are present after ischemia/reperfusion-induced myocardial infarction   (MGI Ref ID J:85798)
      • area of tissue with infarctions visible is only about half that of non-transgenic controls   (MGI Ref ID J:85798)
  • cardiovascular system phenotype
  • altered response to myocardial infarction
    • the mean percentage of apoptotic cells present in the myocardium after ischemia/reperfusion is 14.4% compared to 26.3%   (MGI Ref ID J:85798)
    • myocardial oxidative damage is reduced by almost two-thirds as measured by levels of lipid peroxidation found in the heart after ischemia/reperfusion   (MGI Ref ID J:85798)
    • decreased infarction size
      • only small scattered infarct zones are present after ischemia/reperfusion-induced myocardial infarction   (MGI Ref ID J:85798)
      • area of tissue with infarctions visible is only about half that of non-transgenic controls   (MGI Ref ID J:85798)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Mt1)174Bri
Allele Name transgene insertion 174, Ralph L Brinster
Allele Type Transgenic (random, expressed)
Common Name(s) MT-TG; MTtrans; TgMTI;
Mutation Made By Ralph Brinster,   University of Pennsylvania
Strain of Origin(C57BL/6 x SJL)F2
Expressed Gene Mt1, metallothionein 1, mouse, laboratory
Promoter Mt1, metallothionein 1, mouse, laboratory
Molecular Note The transgene contains mouse metallothionein 1 sequence with 10kb of upstream sequence and 7kb of downstream sequence. 56 copies of the transgene integrated in this line. [MGI Ref ID J:92452]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Mt1)174Bri, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Palmiter RD; Sandgren EP; Koeller DM; Brinster RL. 1993. Distal regulatory elements from the mouse metallothionein locus stimulate gene expression in transgenic mice. Mol Cell Biol 13(9):5266-75. [PubMed: 8355681]  [MGI Ref ID J:92452]

Additional References

Kang YJ; Li Y; Sun X; Sun X. 2003. Antiapoptotic effect and inhibition of ischemia/reperfusion-induced myocardial injury in metallothionein-overexpressing transgenic mice. Am J Pathol 163(4):1579-86. [PubMed: 14507664]  [MGI Ref ID J:85798]

Waelput W; Broekaert D; Vandekerckhove J; Brouckaert P; Tavernier J; Libert C. 2001. A mediator role for metallothionein in tumor necrosis factor-induced lethal shock. J Exp Med 194(11):1617-24. [PubMed: 11733576]  [MGI Ref ID J:73099]

Tg(Mt1)174Bri related

Davis SR; McMahon RJ; Cousins RJ. 1998. Metallothionein knockout and transgenic mice exhibit altered intestinal processing of zinc with uniform zinc-dependent zinc transporter-1 expression. J Nutr 128(5):825-31. [PubMed: 9566988]  [MGI Ref ID J:47419]

Davis SR; Samuelson DA; Cousins RJ. 2001. Metallothionein expression protects against carbon tetrachloride-induced hepatotoxicity, but overexpression and dietary zinc supplementation provide no further protection in metallothionein transgenic and knockout mice. J Nutr 131(2):215-22. [PubMed: 11160536]  [MGI Ref ID J:107225]

Deng DX; Cai L; Chakrabarti S; Cherian MG. 1999. Increased radiation-induced apoptosis in mouse thymus in the absence of metallothionein. Toxicology 134(1):39-49. [PubMed: 10413187]  [MGI Ref ID J:57169]

Ebadi M; Brown-Borg H; El Refaey H; Singh BB; Garrett S; Shavali S; Sharma SK. 2005. Metallothionein-mediated neuroprotection in genetically engineered mouse models of Parkinson's disease. Brain Res Mol Brain Res 134(1):67-75. [PubMed: 15790531]  [MGI Ref ID J:97105]

Ebadi M; Sharma S. 2006. Metallothioneins 1 and 2 attenuate peroxynitrite-induced oxidative stress in Parkinson disease. Exp Biol Med (Maywood) 231(9):1576-83. [PubMed: 17018883]  [MGI Ref ID J:129277]

Giralt M; Penkowa M; Lago N; Molinero A; Hidalgo J. 2002. Metallothionein-1+2 protect the CNS after a focal brain injury. Exp Neurol 173(1):114-28. [PubMed: 11771944]  [MGI Ref ID J:119136]

Iszard MB; Liu J; Liu Y; Dalton T; Andrews GK; Palmiter RD; Klaassen CD. 1995. Characterization of metallothionein-I-transgenic mice. Toxicol Appl Pharmacol 133(2):305-12. [PubMed: 7645027]  [MGI Ref ID J:29102]

Kang YJ; Li Y; Sun X; Sun X. 2003. Antiapoptotic effect and inhibition of ischemia/reperfusion-induced myocardial injury in metallothionein-overexpressing transgenic mice. Am J Pathol 163(4):1579-86. [PubMed: 14507664]  [MGI Ref ID J:85798]

Kelly EJ; Palmiter RD. 1996. A murine model of Menkes disease reveals a physiological function of metallothionein. Nat Genet 13(2):219-22. [PubMed: 8640230]  [MGI Ref ID J:33276]

Lau JC; Cherian MG. 1998. Developmental changes in hepatic metallothionein, zinc, and copper levels in genetically altered mice. Biochem Cell Biol 76(4):615-23. [PubMed: 10099782]  [MGI Ref ID J:53230]

Lee DK; Fu K; Liang L; Dalton T; Palmiter RD; Andrews GK. 1996. Transgenic mouse blastocysts that overexpress metallothionein-I resist cadmium toxicity in vitro. Mol Reprod Dev 43(2):158-66. [PubMed: 8824913]  [MGI Ref ID J:31252]

Molinero A; Penkowa M; Hernandez J; Camats J; Giralt M; Lago N; Carrasco J; Campbell IL; Hidalgo J. 2003. Metallothionein-I overexpression decreases brain pathology in transgenic mice with astrocyte-targeted expression of interleukin-6. J Neuropathol Exp Neurol 62(3):315-28. [PubMed: 12638735]  [MGI Ref ID J:104847]

Ono S; Koropatnick DJ; Cherian MG. 1997. Regional brain distribution of metallothionein, zinc and copper in toxic milk mutant and transgenic mice. Toxicology 124(1):1-10. [PubMed: 9392450]  [MGI Ref ID J:44523]

Oz HS; Chen T; de Villiers WJ; McClain CJ. 2005. Metallothionein overexpression does not protect against inflammatory bowel disease in a murine colitis model. Med Sci Monit 11(3):BR69-73. [PubMed: 15735556]  [MGI Ref ID J:127866]

Sharma SK; El Refaey H; Ebadi M. 2006. Complex-1 activity and 18F-DOPA uptake in genetically engineered mouse model of Parkinson's disease and the neuroprotective role of coenzyme Q10. Brain Res Bull 70(1):22-32. [PubMed: 16750479]  [MGI Ref ID J:112754]

Song Y; Wang J; Li Y; Du Y; Arteel GE; Saari JT; Kang YJ; Cai L. 2005. Cardiac metallothionein synthesis in streptozotocin-induced diabetic mice, and its protection against diabetes-induced cardiac injury. Am J Pathol 167(1):17-26. [PubMed: 15972948]  [MGI Ref ID J:99513]

Waalkes MP; Liu J; Kasprzak KS; Diwan BA. 2004. Minimal influence of metallothionein over-expression on nickel carcinogenesis in mice. Toxicol Lett 153(3):357-64. [PubMed: 15454311]  [MGI Ref ID J:93373]

Waelput W; Broekaert D; Vandekerckhove J; Brouckaert P; Tavernier J; Libert C. 2001. A mediator role for metallothionein in tumor necrosis factor-induced lethal shock. J Exp Med 194(11):1617-24. [PubMed: 11733576]  [MGI Ref ID J:73099]

Wesselkamper SC; McDowell SA; Medvedovic M; Dalton TP; Deshmukh HS; Sartor MA; Case LM; Henning LN; Borchers MT; Tomlinson CR; Prows DR; Leikauf GD. 2006. The role of metallothionein in the pathogenesis of acute lung injury. Am J Respir Cell Mol Biol 34(1):73-82. [PubMed: 16166738]  [MGI Ref ID J:120196]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice can be bred. The expected coat color from breeding is black.
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing
Order this mouse

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $1980.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing
Order this mouse

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2574.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

General Supply Notes

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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