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Strain Name:

B6.129S7-Rag1tm1Mom/J

Stock Number:

002216

Availability:

Level 2

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Genes & Alleles   Rag1;   Rag1tm1Mom;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S7 via AB1 ES cell line (+Hprt-bm2)
Donating Investigator IMR Colony,   The Jackson Laboratory
GenerationN10F34 (20-DEC-06)

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the Rag1tm1Mom mutation produce no mature T cells or B cells. Their phenotype can be described as a "non-leaky" severe combined immune deficiency (Prkdcscid/Prkdcscid) (Prkdcscid mice produce some B cells and IgM). They have no CD3+ or T cell receptor (TCR) alpha-beta positive cells. The thymus of the mutant mice contains 15 to 130 times fewer cells than heterozygous or wildtype siblings. The thymocytes are CD8-CD4- and most are IL2 receptor-positive. Neither the spleen nor the bone marrow contain any IgM or IgD staining cells, indicating an absence of mature B cells. These and other data suggest that B cell and T cell development has been arrested at an early stage. Macroscopically, the mutants are indistinguishable from heterozygotes or normal wildtype siblings.

Strain Development
The Rag1tm1Mom mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A replacement targeting vector with the Pgk-neo marker was used. Homologous recombination of the targeting vector resulted in a 1356 bp deletion in the 5' end of the coding sequence. The 129-derived AB1 ES cell line was used. The C57BL/6J strain was generated by backcrossing mice carrying the Rag1tm1Mom mutation 10 times to C57BL/6J inbred mice.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Rag1tm1Mom/Rag1tm1Mom

        involves: 129S7/SvEvBrd * CD-1
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype (MGI Ref ID J:1934)
    • normal activity, coordination and strength; normal nociception
  • immune system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:1934)
    • spleen and bone marrow lacked mature B cells; B cells were IgM and IgD negative, and only 1/4 to 1/3 were B220-positive
    • defective V(D)J recombination
  • abnormal T cell differentiation (MGI Ref ID J:1934)
    • thymocyte development arrested at an early stage; cells were larger than normal, and were CD3-, TCR alphabeta-negative, CD8- CD4-
  • abnormal inguinal lymph node morphology (MGI Ref ID J:1934)
    • stroma-like structure found in inguinal lymph nodes location, with few, if any, nonerythroid cells recoverable from this structure
  • decreased IgM level (MGI Ref ID J:1934)
    • no detectable IgM found in serum
  • spleen hypoplasia (MGI Ref ID J:1934)
    • spleen contained 5 to 9 times fewer cells than wild type or heterozygous controls
  • thymus hypoplasia (MGI Ref ID J:1934)
    • thymus contained 15 to 300 times fewer cells than wild type or heterozygous controls
  • nervous system phenotype
  • *normal* nervous system phenotype (MGI Ref ID J:1934)
    • brain was normal in structure and size
  • hematopoietic system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:1934)
    • spleen and bone marrow lacked mature B cells; B cells were IgM and IgD negative, and only 1/4 to 1/3 were B220-positive
    • defective V(D)J recombination
  • abnormal T cell differentiation (MGI Ref ID J:1934)
    • thymocyte development arrested at an early stage; cells were larger than normal, and were CD3-, TCR alphabeta-negative, CD8- CD4-
  • spleen hypoplasia (MGI Ref ID J:1934)
    • spleen contained 5 to 9 times fewer cells than wild type or heterozygous controls
  • thymus hypoplasia (MGI Ref ID J:1934)
    • thymus contained 15 to 300 times fewer cells than wild type or heterozygous controls

Rag1tm1Mom/Rag1tm1Mom

        involves: 129S7/SvEvBrd * C57BL/6 * FVB * NOD
  • hematopoietic system phenotype
  • decreased lymphocyte cell number (MGI Ref ID J:111874)
    • non-transgenic Rag1-null mice display lymphopenia more severe than transgenic Rag1-null mice
  • small thymus (MGI Ref ID J:111874)
    • nontransgenic Rag1 homozygotes have extremely small thymi
  • immune system phenotype
  • decreased lymphocyte cell number (MGI Ref ID J:111874)
    • non-transgenic Rag1-null mice display lymphopenia more severe than transgenic Rag1-null mice
  • small thymus (MGI Ref ID J:111874)
    • nontransgenic Rag1 homozygotes have extremely small thymi

Rag1tm1Mom/Rag1tm1Mom

        involves: 129S7/SvEvBrd * C57BL/6
  • immune system phenotype
  • abnormal lymphatic vessel morphology (MGI Ref ID J:119344)
    • upon infection with Mycoplasma pulmonis, airway lymphatic vessel remodeling is largely absent from mutants, compared to significant invasion of region by lymphatic vessels in infected wild-type mice
  • abnormal response to infection (MGI Ref ID J:119344)
    • airway vascular and airway lymphatic vessel remodeling are impaired or absent compared to wild-type mice after M. pulmonis infection
  • decreased neutrophil cell number (MGI Ref ID J:119344)
    • 2 weeks or 1 month after infection with M. pulmonis, neutrophil numbers in the trachea are elevated above uninfected controls but are significantly lower than numbers in trachea of infected wild-type mice
  • hematopoietic system phenotype
  • decreased neutrophil cell number (MGI Ref ID J:119344)
    • 2 weeks or 1 month after infection with M. pulmonis, neutrophil numbers in the trachea are elevated above uninfected controls but are significantly lower than numbers in trachea of infected wild-type mice
  • respiratory system phenotype
  • abnormal respiratory system morphology (MGI Ref ID J:119344)
    • after 4 weeks, mice show impaired vascular remodeling of the airways in response to Mycoplasma pulmonis infection whereas wild-type show complex growth and reorganization ot the vascular beds

Gene & Allele Details

Allele Symbol Rag1tm1Mom
Allele Name targeted mutation 1, Peter Mombaerts
Common Name(s) RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-;
Mutation Made By Peter Mombaerts,   Max Planck Institute of Biophysics
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Rag1, recombination activating gene 1
Chromosome 2
Gene Common Name(s) MGC43321; RNF74; Rag-1;
Molecular Note A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036]

Control Information

  Allele   Control
 Rag1tm1Mom  000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Rag1tm1Mom

Colony Maintenance

Breeding & HusbandryThis Rag1tm1Mom strain is maintained by mating homozygous siblings. This strain should be housed under pathogen free conditions similar to Prkdcscid/Prkdcscid mice or any other immunodeficient strain. Only homozygous mice may be ordered.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   Rag1tm1Mom     (15 strains)

Strains carrying other alleles of Rag1
002194   B6;CBA-Tg(CD3E)26Cpt/J
002506   STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
View Strains carrying other alleles of Rag1     (2 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report
JAX Notes, Spring 2006; 501. Choosing an Immunodeficient Mouse Model.

Animal Health Reports

Room Number           AX30

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Immunology and Inflammation Research (B and T cell deficiency)

Rag1tm1Mom related

Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)

Hematological Research
Immunological Defects (B and T cell deficiency)

Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)

Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)

Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)

References

Selected Reference(s)

Mombaerts P; Iacomini J; Johnson RS; Herrup K; Tonegawa S; Papaioannou VE. 1992. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68(5):869-77. [PubMed: 1547488]  [MGI Ref ID J:1934]

Additional References

Price and Supply Information

Strain Name: B6.129S7-Rag1tm1Mom/J
Stock Number: 002216

Price Details

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Supply Details

Standard SupplyLevel 2. Up to 100 mice. Larger quantities or custom orders arranged upon request.
Supply Notes Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
Strains that must be genotyped are not available until five to seven weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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