Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Richard Hynes,   Massachusetts Institute of Technology

Description
Mice homozygous Selp^tm1Hyn targeted mutation are viable and fertile. Homozygous mutant mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (2-3-fold). Circulating lymphocyte and monocyte counts are normal. It can be used as model for human leukocyte adhesion deficiencies (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).

Development
A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was deleted by the insertion of PGK-neo cassette. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells and this line was maintained on a mixed C57BL/6 and 129S2 background.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Selp^tm1Hyn allele

008441	B6.129S2-Selptm1Hyn Selltm1Hyn/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
003805	B6;129S-Selptm1Hyn Selltm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J
008442	C.129S2(B6)-Selptm1Hyn Selltm1Hyn/J
008433	C.129S2(B6)-Selptm1Hyn/J

View Strains carrying   Selp^tm1Hyn     (7 strains)

Strains carrying other alleles of Selp

002285	B6.129S7-Icam1tm1Bay Selptm1Bay/J
002289	B6.129S7-Selptm1Bay/J

View Strains carrying other alleles of Selp     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Selp^tm1Hyn/Selp^tm1Hyn

        involves: 129S2/SvPas * C57BL/6J

• immune system phenotype
• abnormal leukocyte rolling (MGI Ref ID J:77329)
  • exhibit virtually total absence of leukocyte rolling in mesenteric venules
  • two secretagogues (the calcium ionophore A23187 and hydrogen peroxide) that result in increased rolling in wild-type fail to stimulate any significant rolling in homozygotes
• impaired neutrophil recruitment (MGI Ref ID J:77329)
  • exhibit delayed recruitment of neutrophils to the peritoneal cavity upon experimentally induced inflammation
• increased neutrophil cell number (MGI Ref ID J:77329)
  • exhibit a 2.4-fold increase in circulating neutrophil counts
• hematopoietic system phenotype
• increased neutrophil cell number (MGI Ref ID J:77329)
  • exhibit a 2.4-fold increase in circulating neutrophil counts
• homeostasis/metabolism phenotype
• increased bleeding time (MGI Ref ID J:31431)
  • exhibit a 40% prolongation of the bleeding time on amputation of the tip of the tail
• cardiovascular system phenotype
• hemorrhage (MGI Ref ID J:31431)
  • hemorrhagic skin lesions induced by injection of lipopolysaccharide followed by tumor necrosis factor are larger by a factor of two compared to controls

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Selp^tm1Hyn/Selp^tm1Hyn

        involves: 129S2/SvPas

• immune system phenotype
• abnormal leukocyte rolling (MGI Ref ID J:29814)
  • rolling of leukocytes in subcutaneous vessels is significantly lower under both basal conditions and post-ischemia-reperfusion, but not completely eliminated
  • leukocyte rolling is reduced even four hours after challenge; exhibit significantly fewer rolling cells and their velocity is reduced
• impaired macrophage recruitment (MGI Ref ID J:29814)
  • macrophage recruitment in a peritonitis model is reduced even 48 hours after challenge
• increased neutrophil cell number (MGI Ref ID J:29814)
  • increased numbers of circulating neutrophils
  • clearing of neutrophils from the circulation is delayed in the first 2 to 3 hours after neutrophil injection, but is normal by 5 hours
• hematopoietic system phenotype
• increased neutrophil cell number (MGI Ref ID J:29814)
  • increased numbers of circulating neutrophils
  • clearing of neutrophils from the circulation is delayed in the first 2 to 3 hours after neutrophil injection, but is normal by 5 hours

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Selp^tm1Hyn related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Selptm1Hyn
Allele Name		targeted mutation 1, Richard O Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		P-;
Mutation Made By		Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Selp, selectin, platelet
Chromosome		1
Gene Common Name(s)		CD62; CD62P; FLJ45155; GMP140; GRMP; Grmp; LECAM3; MGC124632; P-selectin; PADGEM; PSEL; PSELECT; granulocyte membrane protein;
Molecular Note		A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was deleted by the insertion of PGK-neo cassette. Mice were treated with lipopolysaccharide to augment the normally low levels of selectin in lung andliver tissue. A longer transcript, resulting from aberrant or cryptic splicing involving the neomycin cassette, was detected at low levels in mutant mice by Northern blot analysis. RT-PCR further confirmed the presence of low levels of transcript in homozygous mice. Immunofluorescence analysis of activated platelets and lung sections from homozygous mutant mice showed an absence of encoded protein. These results were further confirmed by flow cytometric analysis of activated platelets and metabolic labeling of lung tissue. [MGI Ref ID J:77329]

Genotyping

Genotyping Information

Genotyping Protocols

Selp^tm1Hyn, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Mayadas TN; Johnson RC; Rayburn H; Hynes RO; Wagner DD. 1993. Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell 74(3):541-54. [PubMed: 7688665]  [MGI Ref ID J:77329]

Additional References

Johnson RC; Mayadas TN; Frenette PS; Mebius RE; Subramaniam M; Lacasce A; Hynes RO; Wagner DD. 1995. Blood cell dynamics in P-selectin-deficient mice. Blood 86(3):1106-14. [PubMed: 7542495]  [MGI Ref ID J:28190]

Selp^tm1Hyn related

Andre P; Denis CV; Ware J; Saffaripour S; Hynes RO; Ruggeri ZM; Wagner DD. 2000. Platelets adhere to and translocate on von willebrand factor presented by endothelium in stimulated veins Blood 96(10):3322-8. [PubMed: 11071623]  [MGI Ref ID J:65812]

Andre P; Hartwell D; Hrachovinova I; Saffaripour S; Wagner DD. 2000. Pro-coagulant state resulting from high levels of soluble P-selectin in blood. Proc Natl Acad Sci U S A 97(25):13835-40. [PubMed: 11095738]  [MGI Ref ID J:125020]

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Combes V; Rosenkranz AR; Redard M; Pizzolato G; Lepidi H; Vestweber D; Mayadas TN; Grau GE. 2004. Pathogenic role of P-selectin in experimental cerebral malaria: importance of the endothelial compartment. Am J Pathol 164(3):781-6. [PubMed: 14982832]  [MGI Ref ID J:88446]

Connolly ES Jr; Winfree CJ; Prestigiacomo CJ; Kim SC; Choudhri TF; Hoh BL; Naka Y; Solomon RA; Pinsky DJ. 1997. Exacerbation of cerebral injury in mice that express the P-selectin gene: identification of P-selectin blockade as a new target for the treatment of stroke. Circ Res 81(3):304-10. [PubMed: 9285631]  [MGI Ref ID J:43110]

Dole VS; Bergmeier W; Mitchell HA; Eichenberger SC; Wagner DD. 2005. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin. Blood 106(7):2334-9. [PubMed: 15956287]  [MGI Ref ID J:119376]

Egami K; Murohara T; Aoki M; Matsuishi T. 2006. Ischemia-induced angiogenesis: role of inflammatory response mediated by P-selectin. J Leukoc Biol 79(5):971-6. [PubMed: 16641139]  [MGI Ref ID J:108660]

Fernekorn U; Butcher EC; Behrends J; Hartz S; Kruse A. 2004. Functional involvement of P-selectin and MAdCAM-1 in the recruitment of alpha4beta7-integrin-expressing monocyte-like cells to the pregnant mouse uterus. Eur J Immunol 34(12):3423-33. [PubMed: 15484189]  [MGI Ref ID J:94597]

Fernekorn U; Butcher EC; Behrends J; Karsten CM; Robke A; Schulze TJ; Kirchner H; Kruse A. 2007. Selectin, platelet plays a critical role in granulocyte access to the pregnant mouse uterus under physiological and pathological conditions. Biol Reprod 76(4):645-53. [PubMed: 17151351]  [MGI Ref ID J:121141]

Forlow SB; White EJ; Barlow SC; Feldman SH; Lu H; Bagby GJ; Beaudet AL; Bullard DC; Ley K. 2000. Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice J Clin Invest 106(12):1457-66. [PubMed: 11120753]  [MGI Ref ID J:66426]

Frederix K; Chauhan AK; Kisucka J; Zhao BQ; Hoff EI; Spronk HM; Ten Cate H; Wagner DD. 2007. Platelet adhesion receptors do not modulate infarct volume after a photochemically induced stroke in mice. Brain Res 1185:239-45. [PubMed: 17996853]  [MGI Ref ID J:130124]

Frenette J; Chbinou N; Godbout C; Marsolais D; Frenette PS. 2003. Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading. Am J Physiol Regul Integr Comp Physiol 285(4):R727-32. [PubMed: 12829442]  [MGI Ref ID J:109393]

Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043]  [MGI Ref ID J:31626]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Ginhoux F; Collin MP; Bogunovic M; Abel M; Leboeuf M; Helft J; Ochando J; Kissenpfennig A; Malissen B; Grisotto M; Snoeck H; Randolph G; Merad M. 2007. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204(13):3133-46. [PubMed: 18086862]  [MGI Ref ID J:130817]

Gironella M; Molla M; Salas A; Soriano A; Sans M; Closa D; Engel P; Salas A; Pique JM; Panes J. 2002. The role of P-selectin in experimental colitis as determined by antibody immunoblockade and genetically deficient mice. J Leukoc Biol 72(1):56-64. [PubMed: 12101263]  [MGI Ref ID J:124457]

Goerge T; Ho-Tin-Noe B; Carbo C; Benarafa C; Remold-O'Donnell E; Zhao BQ; Cifuni SM; Wagner DD. 2008. Inflammation induces hemorrhage in thrombocytopenia. Blood 111(10):4958-64. [PubMed: 18256319]  [MGI Ref ID J:135316]

Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362]  [MGI Ref ID J:107382]

Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773]  [MGI Ref ID J:50212]

Horie Y; Wolf R; Anderson DC; Granger DN. 1997. Hepatic leukostasis and hypoxic stress in adhesion molecule-deficient mice after gut ischemia/reperfusion. J Clin Invest 99(4):781-8. [PubMed: 9045883]  [MGI Ref ID J:39161]

Johnson RC; Mayadas TN; Frenette PS; Mebius RE; Subramaniam M; Lacasce A; Hynes RO; Wagner DD. 1995. Blood cell dynamics in P-selectin-deficient mice. Blood 86(3):1106-14. [PubMed: 7542495]  [MGI Ref ID J:28190]

Kaifi JT; Hall LR; Diaz C; Sypek J; Diaconu E; Lass JH; Pearlman E. 2000. Impaired eosinophil recruitment to the cornea in P-selectin-deficient mice in onchocerca volvulus keratitis (River blindness) Invest Ophthalmol Vis Sci 41(12):3856-61. [PubMed: 11053286]  [MGI Ref ID J:65551]

Massaguer A; Perez-Del-Pulgar S; Engel P; Serratosa J; Bosch J; Pizcueta P. 2002. Concanavalin-A-induced liver injury is severely impaired in mice deficient in P-selectin. J Leukoc Biol 72(2):262-70. [PubMed: 12149416]  [MGI Ref ID J:124455]

Mayadas TN. 1995. Gene knockout on p-selectin: its biology and function. Trends Cardiovasc Med 5(4):149-157.  [MGI Ref ID J:28867]

Mayadas TN; Mendrick DL; Brady HR; Tang T; Papayianni A; Assmann KJ; Wagner DD; Hynes RO; Cotran RS. 1996. Acute passive anti-glomerular basement membrane nephritis in P-selectin-deficient mice. Kidney Int 49(5):1342-9. [PubMed: 8731099]  [MGI Ref ID J:113194]

McCafferty DM; Kanwar S; Granger DN; Kubes P. 2000. E/P-selectin-deficient mice: an optimal mutation for abrogating antigen but not tumor necrosis factor-alpha-induced immune responses Eur J Immunol 30(8):2362-71. [PubMed: 10940927]  [MGI Ref ID J:63951]

McCafferty DM; Smith CW; Granger DN; Kubes P. 1999. Intestinal inflammation in adhesion molecule-deficient mice: an assessment of P-selectin alone and in combination with ICAM-1 or E-selectin. J Leukoc Biol 66(1):67-74. [PubMed: 10410991]  [MGI Ref ID J:56590]

Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599]  [MGI Ref ID J:134224]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Patrick AL; Rullo J; Beaudin S; Liaw P; Fox-Robichaud AE. 2007. Hepatic leukocyte recruitment in response to time-limited expression of TNF-alpha and IL-1beta. Am J Physiol Gastrointest Liver Physiol 293(4):G663-72. [PubMed: 17656447]  [MGI Ref ID J:126664]

Planck SR; Han YB; Park JM; O'Rourke L; Gutierrez-Ramos JC; Rosenbaum JT. 1998. The effect of genetic deficiency of adhesion molecules on the course of endotoxin-induced uveitis. Curr Eye Res 17(9):941-6. [PubMed: 9746442]  [MGI Ref ID J:114206]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Singh I; Zibari GB; Brown MF; Granger DN; Eppihimer M; Zizzi H; Cruz L; Meyer K; Gonzales E; McDonald JC. 1999. Role of P-selectin expression in hepatic ischemia and reperfusion injury. Clin Transplant 13(1 Pt 2):76-82. [PubMed: 10081641]  [MGI Ref ID J:103151]

Stokol T; O'Donnell P; Xiao L; Knight S; Stavrakis G; Botto M; von Andrian UH; Mayadas TN. 2004. C1q governs deposition of circulating immune complexes and leukocyte Fcgamma receptors mediate subsequent neutrophil recruitment. J Exp Med 200(7):835-46. [PubMed: 15466618]  [MGI Ref ID J:93949]

Subramaniam M; Frenette PS; Saffaripour S; Johnson RC; Hynes RO; Wagner DD. 1996. Defects in hemostasis in P-selectin-deficient mice. Blood 87(4):1238-42. [PubMed: 8608210]  [MGI Ref ID J:31431]

Subramaniam M; Saffaripour S; Watson SR; Mayadas TN; Hynes RO; Wagner DD. 1995. Reduced recruitment of inflammatory cells in a contact hypersensitivity response in P-selectin-deficient mice. J Exp Med 181(6):2277-82. [PubMed: 7539046]  [MGI Ref ID J:110854]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Tang T; Frenette PS; Hynes RO; Wagner DD; Mayadas TN. 1996. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest 97(11):2485-90. [PubMed: 8647940]  [MGI Ref ID J:107411]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Tu L; Poe JC; Kadono T; Venturi GM; Bullard DC; Tedder TF; Steeber DA. 2002. A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. J Immunol 169(4):2034-43. [PubMed: 12165530]  [MGI Ref ID J:120206]

Wang L; Brown JR; Varki A; Esko JD. 2002. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest 110(1):127-36. [PubMed: 12093896]  [MGI Ref ID J:112426]

Yamada S; Mayadas TN; Yuan F; Wagner DD; Hynes RO; Melder RJ; Jain RK. 1995. Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin. Blood 86(9):3487-92. [PubMed: 7579454]  [MGI Ref ID J:29814]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	The Selptm1Hyn strain is maintained as a homozygote. The donating lab maintained these mice by avoiding brother-sister matings. Homozygous mice may be ordered.
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.