Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Frank Chisari,   Scripps Research Institute

Appearance
black
Related Genotype: a/a

Description
This strain is referred to as "50-4" or "Tg(Alb1-HBV)Bri44" in the primary reference.

Control Information

	Control
	Noncarrier female
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Alb

007853	129S/SvEv-Tg(Alb1-Ren)1Unc/CofJ
000186	B6.Cg-Albc/J
007238	B6.Cg-Tg(Alb-PLG)1Dgi/J
007244	B6;SJL-Tg(Alb-F5)2Dgi/J
007245	B6;SJL-Tg(Alb-F5)3Dgi/J

View Strains carrying other alleles of Alb     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(Alb1HBV)44Bri/0

        involves: C57BL/6J * SJL/J

• tumorigenesis
• liver tumor (MGI Ref ID J:86165)
  • 9-12 months after the onset of hepatocellular injury, mutants develop liver tumors, with all mice developing hepatocellular neoplasms by 18-21 months of age
  • multiple tumors of various sizes are present in the same liver and tumors are highly vascularized
  • liver tumors develop more rapidly in mice derived from transgenic by nontransgenic (C57BL/6J x SJL/J) matings than in mice derived by repeated backcrossing against C57BL/6J mice
  • in mutants backcrossed against C57BL/6J, males develop liver tumors more rapidly than females
  • younger males tend to harbor adenomas, while carcinomas are more common in older males
• hepatocellular carcinoma (MGI Ref ID J:86165)
• liver adenoma (MGI Ref ID J:86165)
  • hepatocellular adenomas
• liver/biliary system phenotype
• abnormal liver morphology (MGI Ref ID J:86165)
  • mutants develop hepatocellular injury at around 2-3 months of age, followed by hyperplasia and neoplasia

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Death Receptors

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Increased Tumor Incidence
      Hepatomas: hepatacellular carcinoma

Virology Research
Hepatitis B Virus

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Alb1HBV)44Bri
Allele Name		transgene insertion 44, Ralph L Brinster
Allele Type		Transgenic (random, expressed)
Common Name(s)		50-4; 50.4; HBs-tg; HBsAg; Tg(Alb1-HBV)Bri44;
Mutation Made By		Frank Chisari,   Scripps Research Institute
Expressed Gene		HBV, hepatitis B virus large envelope polypeptide,
Promoter		Alb, albumin, mouse, laboratory
Molecular Note		Sequence encoding the hepatitis B virus large envelope polypeptide was placed downstream of the mouse albumin promoter, thereby expressing the peptide in hepatocytes. [MGI Ref ID J:86165]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Alb1HBV)44Bri, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Chisari FV; Klopchin K; Moriyama T; Pasquinelli C; Dunsford HA; Sell S; Pinkert CA; Brinster RL; Palmiter RD. 1989. Molecular pathogenesis of hepatocellular carcinoma in hepatitis B virus transgenic mice. Cell 59(6):1145-56. [PubMed: 2598264]  [MGI Ref ID J:86165]

Additional References

McGlynn KA; Hunter K; LeVoyer T; Roush J; Wise P; Michielli RA; Shen FM; Evans AA; London WT; Buetow KH. 2003. Susceptibility to aflatoxin B1-related primary hepatocellular carcinoma in mice and humans. Cancer Res 63(15):4594-601. [PubMed: 12907637]  [MGI Ref ID J:85696]

Singh M; Kumar V. 2003. Transgenic mouse models of hepatitis B virus-associated hepatocellular carcinoma. Rev Med Virol 13(4):243-53. [PubMed: 12820186]  [MGI Ref ID J:84502]

Tg(Alb1HBV)44Bri related

Bao JJ; Lee BP; Stephens LC; Sahin AA; Van NT; Johnston DA; Ou CN; Kuo MT. 2000. Elevated expression of hepatic proliferative markers during early hepatocarcinogenesis in hepatitis-B virus transgenic mice lacking mdr1a-encoded P-glycoprotein Mol Carcinog 29(2):103-11. [PubMed: 11074607]  [MGI Ref ID J:66205]

Barone M; Spano D; D'Apolito M; Centra M; Lasalandra C; Capasso M; Di Leo A; Volinia S; Arcelli D; Rosso N; Francavilla A; Tiribelli C; Iolascon A. 2006. Gene expression analysis in HBV transgenic mouse liver: a model to study early events related to hepatocarcinogenesis. Mol Med 12(4-6):115-23. [PubMed: 16953557]  [MGI Ref ID J:115973]

Chemin I; Ohgaki H; Chisari FV; Wild CP. 1999. Altered expression of hepatic carcinogen metabolizing enzymes with liver injury in HBV transgenic mouse lineages expressing various amounts of hepatitis B surface antigen. Liver 19(2):81-7. [PubMed: 10220736]  [MGI Ref ID J:91033]

Chen Y; Wei H; Sun R; Tian Z. 2005. Impaired function of hepatic natural killer cells from murine chronic HBsAg carriers. Int Immunopharmacol 5(13-14):1839-52. [PubMed: 16275620]  [MGI Ref ID J:103638]

Chisari FV; Filippi P; Buras J; McLachlan A; Popper H; Pinkert CA; Palmiter RD; Brinster RL. 1987. Structural and pathological effects of synthesis of hepatitis B virus large envelope polypeptide in transgenic mice. Proc Natl Acad Sci U S A 84(19):6909-13. [PubMed: 3477814]  [MGI Ref ID J:94462]

Crawford DR; Ostrowski S; Vakharia D; Ilic Z; Sell S. 2006. Separate Origins of Hepatitis B Virus Surface Antigen-Negative Foci and Hepatocellular Carcinomas in Transgenic HBsAg (alb/psx) Mice. Am J Pathol 169(1):223-32. [PubMed: 16816375]  [MGI Ref ID J:110171]

Dunsford HA; Sell S; Chisari FV. 1990. Hepatocarcinogenesis due to chronic liver cell injury in hepatitis B virus transgenic mice. Cancer Res 50(11):3400-7. [PubMed: 1692259]  [MGI Ref ID J:101631]

Ghebranious N; Sell S. 1998. Hepatitis B injury, male gender, aflatoxin, and p53 expression each contribute to hepatocarcinogenesis in transgenic mice. Hepatology 27(2):383-91. [PubMed: 9462635]  [MGI Ref ID J:47062]

Ghebranious N; Sell S. 1998. The mouse equivalent of the human p53ser249 mutation p53ser246 enhances aflatoxin hepatocarcinogenesis in hepatitis B surface antigen transgenic and p53 heterozygous null mice. Hepatology 27(4):967-73. [PubMed: 9537435]  [MGI Ref ID J:47088]

Huang SN; Chisari FV. 1995. Strong, sustained hepatocellular proliferation precedes hepatocarcinogenesis in hepatitis B surface antigen transgenic mice. Hepatology 21(3):620-6. [PubMed: 7875658]  [MGI Ref ID J:90880]

Kaplanski C; Chisari FV; Wild CP. 1997. Minisatellite rearrangements are increased in liver tumours induced by transplacental aflatoxin B1 treatment of hepatitis B virus transgenic mice, but not in spontaneously arising tumours. Carcinogenesis 18(4):633-9. [PubMed: 9111192]  [MGI Ref ID J:39821]

Kuo MT; Zhao JY; Teeter LD; Ikeguchi M; Chisari FV. 1992. Activation of multidrug resistance (P-glycoprotein) mdr3/mdr1a gene during the development of hepatocellular carcinoma in hepatitis B virus transgenic mice. Cell Growth Differ 3(8):531-40. [PubMed: 1356418]  [MGI Ref ID J:3153]

Lechel A; Holstege H; Begus Y; Schienke A; Kamino K; Lehmann U; Kubicka S; Schirmacher P; Jonkers J; Rudolph KL. 2007. Telomerase deletion limits progression of p53-mutant hepatocellular carcinoma with short telomeres in chronic liver disease. Gastroenterology 132(4):1465-75. [PubMed: 17433324]  [MGI Ref ID J:128326]

McGlynn KA; Hunter K; LeVoyer T; Roush J; Wise P; Michielli RA; Shen FM; Evans AA; London WT; Buetow KH. 2003. Susceptibility to aflatoxin B1-related primary hepatocellular carcinoma in mice and humans. Cancer Res 63(15):4594-601. [PubMed: 12907637]  [MGI Ref ID J:85696]

Qu X; Yu J; Bhagat G; Furuya N; Hibshoosh H; Troxel A; Rosen J; Eskelinen EL; Mizushima N; Ohsumi Y; Cattoretti G; Levine B. 2003. Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J Clin Invest 112(12):1809-20. [PubMed: 14638851]  [MGI Ref ID J:86953]

Reifenberg K; Hildt E; Lecher B; Wiese E; Nusser P; Ott S; Yamamura K; Rutter G; Lohler J. 2006. IFNgamma expression inhibits LHBs storage disease and ground glass hepatocyte appearance, but exacerbates inflammation and apoptosis in HBV surface protein-accumulating transgenic livers. Liver Int 26(8):986-93. [PubMed: 16953839]  [MGI Ref ID J:135035]

Roh S; Kim K. 2003. Overcoming tolerance in hepatitis B virus transgenic mice: a possible involvement of regulatory T cells. Microbiol Immunol 47(6):453-60. [PubMed: 12906106]  [MGI Ref ID J:127655]

Schirmbeck R; Dikopoulos N; Kwissa M; Leithauser F; Lamberth K; Buus S; Melber K; Reimann J. 2003. Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants. Eur J Immunol 33(12):3342-52. [PubMed: 14635042]  [MGI Ref ID J:87140]

Sun D; Ren H; Oertel M; Sellers RS; Zhu L. 2007. Loss of p27Kip1 enhances tumor progression in chronic hepatocyte injury-induced liver tumorigenesis with widely ranging effects on Cdk2 or Cdc2 activation. Carcinogenesis 28(9):1859-66. [PubMed: 17434927]  [MGI Ref ID J:125423]

Trobonjaca Z; Kroger A; Stober D; Leithauser F; Moller P; Hauser H; Schirmbeck R; Reimann J. 2002. Activating immunity in the liver. II. IFN-beta attenuates NK cell-dependent liver injury triggered by liver NKT cell activation. J Immunol 168(8):3763-70. [PubMed: 11937527]  [MGI Ref ID J:126190]

Wiemann SU; Satyanarayana A; Buer J; Kamino K; Manns MP; Rudolph KL. 2005. Contrasting effects of telomere shortening on organ homeostasis, tumor suppression, and survival during chronic liver damage. Oncogene 24(9):1501-9. [PubMed: 15608677]  [MGI Ref ID J:96885]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier female
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

OncoMouse^® requires a license from DuPont, see Licenses for Strains with OncoMouse^® Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.