Strain Name:

C57BL/6J-Tg(LckIl4)1315Dbl/J

Stock Number:

002230

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationN?+17
 
Donating Investigator Roger Perlmutter,   Amgen

Appearance
black
Related Genotype: a/a

Description
Transgenic mice overexpressing the IL4 transgene under the control of the Lck promoter show severe osteoporosis of both cortical and trabecular bone by 2 months in both males and females. There is decreased bone formation by osteoblasts and kyphosis; characterized by long snout ("weasel face") by 6 weeks of age.

Control Information

  Control
   Noncarrier female
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Il4     (11 strains)

Strains carrying other alleles of Lck
002817   B6.129S2-Lcktm1Mak/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
002427   C3H/He-Tg(LCKprBCL2)36Sjk/J
003332   C57BL/6-Tg(LckNotch1)9Erob/J
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
View Strains carrying other alleles of Lck     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(LckIl4)1315Dbl/0

        involves: C57BL/6J * DBA/2J
  • immune system phenotype
  • abnormal T cell differentiation (MGI Ref ID J:92453)
    • an increased proportion of CD3+ thymocytes co-express alphabeta TCR in the thymus of 6 week old mice
    • abnormal T cell activation (MGI Ref ID J:92453)
      • CD4 T cells in the spleen or lymph nodes have significantly less calcium flux when activated
      • abnormal thymocyte activation (MGI Ref ID J:92453)
        • double positive thymocytes have greater increases in calcium concentration flux when activated through their TCR complex
        • single positive thymocytes have dramatically lower fluxes in intracellular calcium concentrations when activated through their TCR complex
    • decreased double-positive T cell number (MGI Ref ID J:92453)
      • the proportion and absolute number of double positive T cells is greatly reduced in the thymus
    • increased double-negative T cell number (MGI Ref ID J:92453)
      • the proportion but not absolute number of double negative T cells found in the thymus is increased
  • abnormal T cell number (MGI Ref ID J:92453)
    • absent CD8-positive T cells (MGI Ref ID J:92453)
      • CD8 T cells are absent in the spleen, lymph nodes, and in circulation
    • decreased CD4-positive T cell number (MGI Ref ID J:92453)
      • there is a 5-fold drop in the number of CD4 T cells found in lymph nodes
    • decreased double-positive T cell number (MGI Ref ID J:92453)
      • the proportion and absolute number of double positive T cells is greatly reduced in the thymus
    • increased CD4-positive T cell number (MGI Ref ID J:92453)
      • the proportion but not absolute number of CD4 T cells found in the thymus is increased
    • increased CD8-positive T cell number (MGI Ref ID J:92453)
      • the proportion and absolute number of CD8 T cells is greatly increased in the thymus
    • increased double-negative T cell number (MGI Ref ID J:92453)
      • the proportion but not absolute number of double negative T cells found in the thymus is increased
  • abnormal osteoclast physiology (MGI Ref ID J:107359)
    • tartate-resistant acid phosphatase activity by osteoclasts is markedly reduced
  • abnormal thymus morphology (MGI Ref ID J:92453)
    • abnormal thymus cell ratio (MGI Ref ID J:92453)
      • the ratio between CD4-CD8+/ CD4+CD8- T cells exceeds 1 compared to 0.3 in controls
    • thymus hypoplasia (MGI Ref ID J:92453)
      • thymus is visibly smaller with 4 to 5 fold less cells than controls
      • thymic cortex hypoplasia (MGI Ref ID J:92453)
        • thymus lacks a clearly defined cortical region with thymocytes spread out through the medulla in a loosely packed manner
  • increased IgG2a level (MGI Ref ID J:92453)
    • mice infected with Syphacia obvelata pinworm have higher serum levels of IgG2a
  • increased interleukin-4 secretion (MGI Ref ID J:92453)
    • unstimulated thymocytes isolated from mice as young as E18 secrete IL-4 in culture
    • the amount of IL-4 secreted is 25 to 70 pg/ml over a 24 hour period
  • increased susceptibility to parasitic infection (MGI Ref ID J:92453)
    • conventionally housed transgenic mice in one facility invariably developed severe intestinal infection with the Syphacia obvelata pinworm
    • megacolon, rectal prolapse, and cecal perforation were frequent with this infection and mice invariably died between 6-12 weeks of age
  • skeleton phenotype
  • abnormal cancellous bone morphology (MGI Ref ID J:107359)
    • trabecular mass in vertebrae and long bones is substantially decreased
  • abnormal osteoblast physiology (MGI Ref ID J:107359)
    • osteocalcin level in sera are half that of wild-type mice indicating less osteoblast activity
    • there is a profound decrease in alkaline phosphatase activity of osteoblasts lining the periosteum, endosteum, and trabeculae
    • percent of bone surface labeled in vivo with tetracycline is 32.2% versus 49.0% in wild-type controls
  • abnormal osteoclast physiology (MGI Ref ID J:107359)
    • tartate-resistant acid phosphatase activity by osteoclasts is markedly reduced
  • decreased bone strength (MGI Ref ID J:107359)
    • both the stiffness and the failure load of femurs is significantly reduced
  • decreased cortical bone thickness (MGI Ref ID J:107359)
    • cortical thinning of the skeleton occurs by 2 months of age with bones looking translucent upon necropsy
    • there is a significant reduction in the mean cortical thickness and percent cortical area at the midshaft of the radius and the ulna
  • increased diameter of femur (MGI Ref ID J:107359)
    • the anterior-posterior cross section of femurs is significantly increased
    • the medial-lateral cross section is not increased giving the bones a more rounded appearance
  • kyphosis (MGI Ref ID J:107359)
    • between 3 to 6 months of age, mice develop an exaggerated curvature of the spine
  • osteoporosis (MGI Ref ID J:107359)
    • decreased bone mass but no excess of unmineralized matrix indicates these mice are osteoperotic
  • homeostasis/metabolism phenotype
  • hyperphosphatemia (MGI Ref ID J:107359)
    • mice are slightly hyperphosphatemic with values of 9.2 mg/dl compared to 8.1 mg/dl in controls
  • limbs/digits/tail phenotype
  • increased diameter of femur (MGI Ref ID J:107359)
    • the anterior-posterior cross section of femurs is significantly increased
    • the medial-lateral cross section is not increased giving the bones a more rounded appearance
  • hematopoietic system phenotype
  • abnormal T cell differentiation (MGI Ref ID J:92453)
    • an increased proportion of CD3+ thymocytes co-express alphabeta TCR in the thymus of 6 week old mice
    • abnormal T cell activation (MGI Ref ID J:92453)
      • CD4 T cells in the spleen or lymph nodes have significantly less calcium flux when activated
      • abnormal thymocyte activation (MGI Ref ID J:92453)
        • double positive thymocytes have greater increases in calcium concentration flux when activated through their TCR complex
        • single positive thymocytes have dramatically lower fluxes in intracellular calcium concentrations when activated through their TCR complex
    • decreased double-positive T cell number (MGI Ref ID J:92453)
      • the proportion and absolute number of double positive T cells is greatly reduced in the thymus
    • increased double-negative T cell number (MGI Ref ID J:92453)
      • the proportion but not absolute number of double negative T cells found in the thymus is increased
  • abnormal T cell number (MGI Ref ID J:92453)
    • absent CD8-positive T cells (MGI Ref ID J:92453)
      • CD8 T cells are absent in the spleen, lymph nodes, and in circulation
    • decreased CD4-positive T cell number (MGI Ref ID J:92453)
      • there is a 5-fold drop in the number of CD4 T cells found in lymph nodes
    • decreased double-positive T cell number (MGI Ref ID J:92453)
      • the proportion and absolute number of double positive T cells is greatly reduced in the thymus
    • increased CD4-positive T cell number (MGI Ref ID J:92453)
      • the proportion but not absolute number of CD4 T cells found in the thymus is increased
    • increased CD8-positive T cell number (MGI Ref ID J:92453)
      • the proportion and absolute number of CD8 T cells is greatly increased in the thymus
    • increased double-negative T cell number (MGI Ref ID J:92453)
      • the proportion but not absolute number of double negative T cells found in the thymus is increased
  • abnormal thymus morphology (MGI Ref ID J:92453)
    • abnormal thymus cell ratio (MGI Ref ID J:92453)
      • the ratio between CD4-CD8+/ CD4+CD8- T cells exceeds 1 compared to 0.3 in controls
    • thymus hypoplasia (MGI Ref ID J:92453)
      • thymus is visibly smaller with 4 to 5 fold less cells than controls
      • thymic cortex hypoplasia (MGI Ref ID J:92453)
        • thymus lacks a clearly defined cortical region with thymocytes spread out through the medulla in a loosely packed manner
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Il4 related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(LckIl4)1315Dbl
Allele Name transgene insertion 1315, David Lewis
Allele Type Transgenic (random, expressed)
Mutation Made By David Lewis,   University of Washington
Strain of Origin(C57BL/6J x DBA/2J)F2
Expressed Gene Il4, interleukin 4, mouse, laboratory
Promoter Lck, lymphocyte protein tyrosine kinase, mouse, laboratory
Molecular Note Transgenic mice overexpress the IL4 transgene under the control of the Lck promoter (lymphocyte protein tyrosine kinase). 8 copies of the transgene were estimated to have integrated into the genome. Expression of the transgene was detected in thymus tissue and by thymocytes. [MGI Ref ID J:92453]

Genotyping

Genotyping Information

Genotyping Protocols

Human Growth Hormone Polyadenylation Signal Sequence, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Lewis DB; Yu CC; Forbush KA; Carpenter J; Sato TA; Grossman A; Liggitt DH; Perlmutter RM. 1991. Interleukin 4 expressed in situ selectively alters thymocyte development. J Exp Med 173(1):89-100. [PubMed: 1824637]  [MGI Ref ID J:92453]

Additional References

Tg(LckIl4)1315Dbl related

Izbicki G; Or R; Christensen TG; Segel MJ; Fine A; Goldstein RH; Breuer R. 2002. Bleomycin-induced lung fibrosis in IL-4-overexpressing and knockout mice. Am J Physiol Lung Cell Mol Physiol 283(5):L1110-6. [PubMed: 12376365]  [MGI Ref ID J:107354]

Lewis DB; Liggitt HD; Effmann EL; Motley ST; Teitelbaum SL; Jepsen KJ; Goldstein SA; Bonadio J; Carpenter J; Perlmutter RM. 1993. Osteoporosis induced in mice by overproduction of interleukin 4. Proc Natl Acad Sci U S A 90(24):11618-22. [PubMed: 8265598]  [MGI Ref ID J:107359]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier female
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


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Contact Information
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Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

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