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Strain Name:

B6.129-Apoetm1Unc Ldlrtm1Her/J

Stock Number:

002246

Availability:

Repository-Cryopreserved

Price and Supply Information

General Terms and Conditions

Genes & Alleles   Apoe;   Apoetm1Unc;   Ldlr;   Ldlrtm1Her;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129P2 via E14TG2a ES cell line and 129S7 via AB1 ES cell line (+Hprt-bm2)
Donating Investigator The Jackson Laboratory,  
GenerationF15p (25-JUL-04)

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the Apoetm1Unc and Ldlrtm1Hertargeted mutations on a normal chow diet show cholesterol levels corresponding to those seen in the Apoetm1Unc targeted mutant strain. The lipoprotein pattern in homozygous mice also resembles that seen in the Apoetm1Unc targeted mutant strain (VLDL & chylomicron remnants). The double targeted mice have a marked increase in both APOB48 and APOB100. In addition, APOAIV is increased.

Strain Development
This double mutant was made by mating the B6.129P2-Apoetm1Unc mutant strain (Stock number 002052) at backcross generation B6N10 and the B6.129S7-Ldlrtm1Her mutant strain (Stock number 002207) at backcross generation B6N10.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Apoetm1Unc/Apoetm1Unc Ldlrtm1Her/Ldlrtm1Her

        involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J
  • cardiovascular system phenotype
  • abnormal impulse conducting system conduction (MGI Ref ID J:95598)
    • abnormal ST interval (MGI Ref ID J:95598)
      • elevated STU segment as a result of induced hypoxia
      • noticeable at 16% oxygen concentration
      • ECG is corrected by 10 minutes after restoration of normal oxygen
      • hypoxia response unaffected by treatment with a thrombin inhibitor
  • altered response to myocardial infarction (MGI Ref ID J:95598)
    • mice treated with a thrombin inhibitor display lower Troponin-T levels and fewer fibrinogen + cells than untreated mice 48 hours after hypoxia
    • decreased infarction size (MGI Ref ID J:95598)
      • in mice treated with a thrombin inhibitor 48 hours after hypoxia
      • infarctions are irregular in shape and subendocardial or intramural
  • atherosclerotic lesions (MGI Ref ID J:95598)
    • two out of 8 hypoxic hearts show thrombosis as well as atherosclerotic plaques
  • homeostasis/metabolism phenotype
  • altered response to myocardial infarction (MGI Ref ID J:95598)
    • mice treated with a thrombin inhibitor display lower Troponin-T levels and fewer fibrinogen + cells than untreated mice 48 hours after hypoxia
    • decreased infarction size (MGI Ref ID J:95598)
      • in mice treated with a thrombin inhibitor 48 hours after hypoxia
      • infarctions are irregular in shape and subendocardial or intramural
  • thrombosis (MGI Ref ID J:95598)

Gene & Allele Details

Allele Symbol Apoetm1Unc
Allele Name targeted mutation 1, University of North Carolina
Common Name(s) APOE KO; AopE(-); ApoE-KO; Apoetm1Un; apoE-; apoE0; epsilon-;
Mutation Made By Nobuyo Maeda,   Univ of North Carolina at Chapel Hill
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Apoe, apolipoprotein E
Chromosome 7
Gene Common Name(s) AD2; AI255918; APOEA; LPG; MGC1571; apoprotein; expressed sequence AI255918;
General Note

In Apoetm1Unc mutant mice also carrying Csf1op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:40136). The results may have been due to reduced macrophages or M-CSF, but were not due to reductions in circulating lipoprotein (J:40136).

Molecular Note Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. [MGI Ref ID J:1050]
 
Allele Symbol Ldlrtm1Her
Allele Name targeted mutation 1, Joachim Herz
Common Name(s) LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her;
Mutation Made By Joachim Herz,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Ldlr, low density lipoprotein receptor
Chromosome 9
Gene Common Name(s) FH; FHC; LDLRA;
General Note When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777)
Molecular Note Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Apoetm1Unc
Ldlr tm1Her

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   Apoetm1Unc     (9 strains)

View Strains carrying   Ldlrtm1Her     (11 strains)

Strains carrying other alleles of Ldlr
005061   C57BL/6J-LdlrHlb301/J
View Strains carrying other alleles of Ldlr     (1 strain)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Neurodegeneration

Apoetm1Unc related

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia

Mouse/Human Gene Homologs
Alzheimer's
hyperlipoproteinemia, type III

Ldlrtm1Her related

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia

Metabolism Research
Lipid Metabolism

Mouse/Human Gene Homologs
hypercholesterolemia, familial

References

Selected Reference(s)

Ishibashi S; Herz J; Maeda N; Goldstein JL; Brown MS. 1994. The two-receptor model of lipoprotein clearance: tests of the hypothesis in knockout mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins. Proc Natl Acad Sci U S A 91(10):4431-5. [PubMed: 8183926]  [MGI Ref ID J:18138]

Additional References

Price and Supply Information

Strain Name: B6.129-Apoetm1Unc Ldlrtm1Her/J
Stock Number: 002246

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)
Cryorecovery Fee $2470.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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