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Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S2 via D3 ES cell line Generation N?+10F14 (05-DEC-07) Donating Investigator IMR Colony, The Jackson Laboratory Appearance
black
Related Genotype: a/aDescription
Mice homozygous Ltatm1Dch targeted mutation are viable and fertile. Homozygous mutant mice show abnormal development of peripheral lymphoid organs with no detectable popliteal, inguinal, para-aortic, mesenteric, axillary, or cervical lymph nodes, and no detectable Peyer's patches. Morphological changes in the spleen white pulp were accompanied by alterations in T and B cell content. CD4+ and CD8+ T cells counts in peripheral blood are normal but there is a four-fold increase in B cells. Neutrophil, monocyte, and platelet counts are normal. The thymus contains normal numbers of CD4+CD8+, CD4+, CD8+, and CD4-CD8- T cells. Splenic T cells develop normal MHC class I and class II-restricted allocytotoxic responses. Also known as tumor necrosis factor beta, Tnfb.Development
The targeting strategy resulted in the elemination of exon 3 (4 exons total) and the introduction of a stop codon in exon 2. The predominant 1.5 and 1.8 kb Lta transcripts are missing and a major 3 kb transcript consistent with the structure of the targeted gene is present. 129 derived D3 ES cells were used for the targeting. The Ltatm1Dch targeted mutation was backcrossed to the C57BL/6J inbred strain for 5 generations (N5) prior to establishing a homozygous colony. Backcrossing is continuing to N10.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying Ltatm1Dch allele
002257 B6;129S2-Ltatm1Dch/J View Strains carrying Ltatm1Dch (1 strain)
Strains carrying other alleles of Lta
005108 B6.129P2-Ltb/Tnf/Ltatm1Dvk/J 005579 C57BL/6J-Ltahlb382/J View Strains carrying other alleles of Lta (2 strains)
Congenic Nomenclature
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
Ltatm1Dch/Ltatm1Dch
B6.129S2-Ltatm1Dch
- immune system phenotype
- abnormal immune serum protein physiology (MGI Ref ID J:80616)
- abnormal cytokine secretion (MGI Ref ID J:95222)
- chemokine expression is essentially absent
- no Ifn-gamma produced in nasal-associated lymphoid tissue but it is found in the spleen
- decreased IgG level (MGI Ref ID J:80616)
- defecient IgG antibody production
- increased IgM level (MGI Ref ID J:80616)
- 2-fold increased IgM
- abnormal mucosa-associated lymphoid tissue morphology (MGI Ref ID J:80616)
- nasal-associated lymphoid tissue poorly developed and hypocellular
- absent Peyer's patches (MGI Ref ID J:80616)
- not grossly detectable
- absent spleen germinal center (MGI Ref ID J:80616)
- absent spleen marginal zone (MGI Ref ID J:80616)
- decreased spleen white pulp amount (MGI Ref ID J:80616)
- increased leukocyte cell number (MGI Ref ID J:80616)
- 2- to 3- fold increased leukocyte count in spleen, blood, and peritoneal cavity
- hematopoietic system phenotype
- absent spleen germinal center (MGI Ref ID J:80616)
- absent spleen marginal zone (MGI Ref ID J:80616)
- decreased spleen white pulp amount (MGI Ref ID J:80616)
- increased leukocyte cell number (MGI Ref ID J:80616)
- 2- to 3- fold increased leukocyte count in spleen, blood, and peritoneal cavity
Ltatm1Dch/Ltatm1Dch
B6.129S2-Ltatm1Dch/J
- immune system phenotype
- abnormal lymph organ development (MGI Ref ID J:73100)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ltatm1Dch/Ltatm1Dch
involves: 129S2/SvPas * C57BL/6
- life span-post-weaning/aging
- abnormal induced morbidity/mortality (MGI Ref ID J:109621)
- in response to LPS and D-GalN administration, percentage of severely moribund mice is 0 at 7 hours; 3/7 mice are severely moribund at 8 hours
- immune system phenotype
- abnormal class switch recombination (MGI Ref ID J:109621)
- 19 days after immunization with OVA, mice display an isotype switch to IgG from IgM that is several fold decreased compared to wild-type
- abnormal humoral immune response (MGI Ref ID J:39746)
- in response to stimulation with sheep red blood cells (SRBC), mice fail to produce anti-SRBC IgG at day 6 and only produce a negligible amount by day 15 unlike wild-type mice
- abnormal leukocyte cell number (MGI Ref ID J:17976)
- decreased T cell number (MGI Ref ID J:17976)
- in the spleen
- increased leukocyte cell number (MGI Ref ID J:17976)
- nearly 3 fold increase
- neutrophil, monocyte, platelet counts normal
- increased B cell number (MGI Ref ID J:17976)
- 4 fold increase in B cell numbers
- 30% increase in IgM positive and B220 positive B lymphocytes
- abnormal lymph node morphology (MGI Ref ID J:17976)
- lack para-aortic lymph nodes
- abnormal axillary lymph node morphology (MGI Ref ID J:17976)
- absent
- abnormal cervical lymph node morphology (MGI Ref ID J:17976)
- absent
- abnormal inguinal lymph node morphology (MGI Ref ID J:17976)
- absent
- abnormal lymph node primary follicle (MGI Ref ID J:109621)
- only occasional BP-3 cells are found in some lymphoid follicles
- abnormal popliteal lymph node morphology (MGI Ref ID J:17976)
- absent
- absent mesenteric lymph nodes (MGI Ref ID J:17976)
- mice lack mesenteric and cervical lymph nodes
- abnormal spleen morphology (MGI Ref ID J:17976)
- mice exhibit extensive disorganization
- abnormal spleen periarteriolar lymphoid sheath morphology (MGI Ref ID J:17976)
- absent spleen marginal zone (MGI Ref ID J:17976)
- loss of a distinct marginal zone
- decreased spleen white pulp amount (MGI Ref ID J:109621)
- percentage of splenic white pulp is lower than in wild-type, Ltb nulls and Ltatm1.1Sned but higher than Ltb, Ltbr or Ltb/Tnf/Lta knockouts
- absent Peyer's patches (MGI Ref ID J:17976)
- not grossly detectable
- decreased interferon-gamma secretion (MGI Ref ID J:109621)
- in culture, OVA-specific Ifng production from purified CD4+ T cells is decreased 2-fold compared to Ltatm1.1Sned
- decreased tumor necrosis factor secretion (MGI Ref ID J:109621)
- in response to LPS, mice demonstrate a 10-fold decrease in production of TNF compared to wild-type or Lta
- both bone marrow derived macrophages and neutrophils fail to produce normal levels of TNF when stimulated
- hematopoietic system phenotype
- abnormal class switch recombination (MGI Ref ID J:109621)
- 19 days after immunization with OVA, mice display an isotype switch to IgG from IgM that is several fold decreased compared to wild-type
- abnormal leukocyte cell number (MGI Ref ID J:17976)
- decreased T cell number (MGI Ref ID J:17976)
- in the spleen
- increased leukocyte cell number (MGI Ref ID J:17976)
- nearly 3 fold increase
- neutrophil, monocyte, platelet counts normal
- increased B cell number (MGI Ref ID J:17976)
- 4 fold increase in B cell numbers
- 30% increase in IgM positive and B220 positive B lymphocytes
- abnormal spleen morphology (MGI Ref ID J:17976)
- mice exhibit extensive disorganization
- abnormal spleen periarteriolar lymphoid sheath morphology (MGI Ref ID J:17976)
- absent spleen marginal zone (MGI Ref ID J:17976)
- loss of a distinct marginal zone
- decreased spleen white pulp amount (MGI Ref ID J:109621)
- percentage of splenic white pulp is lower than in wild-type, Ltb nulls and Ltatm1.1Sned but higher than Ltb, Ltbr or Ltb/Tnf/Lta knockouts
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Ltatm1Dch related
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
| Allele Symbol | Ltatm1Dch | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, David D Chaplin | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | LT-; LT-alpha-; LTalpha-; | ||
| Mutation Made By | David Chaplin, University of Alabama at Birmingham | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Lta, lymphotoxin A | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | LT; LT-[a]; LT-alpha; LT[a]; LTalpha; Ltx; TNF beta; TNF-beta; TNFB; TNFSF1; Tnfb; Tnfsf1b; heart, lung and blood 382; hlb382; lymphotoxin; lymphotoxin alpha; tumor necrosis factor beta; | ||
| Molecular Note | A neomycin resistance cassette replaced a small portion of exon 2, all of exon 3, and a small portion of exon 4. [MGI Ref ID J:110548] [MGI Ref ID J:17976] [MGI Ref ID J:95222] | ||
Genotyping Protocols
Ltatm1Dch, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
De Togni P; Goellner J; Ruddle NH; Streeter PR; Fick A; Mariathasan S; Smith SC; Carlson R; Shornick LP; Strauss-Schoenberger J; Russell JH; Karr R; Chaplin DD. 1994. Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin [see comments] Science 264(5159):703-7. [PubMed: 8171322] [MGI Ref ID J:17976]
Jang MH; Kweon MN; Iwatani K; Yamamoto M; Terahara K; Sasakawa C; Suzuki T; Nochi T; Yokota Y; Rennert PD; Hiroi T; Tamagawa H; Iijima H; Kunisawa J; Yuki Y; Kiyono H. 2004. Intestinal villous M cells: an antigen entry site in the mucosal epithelium. Proc Natl Acad Sci U S A 101(16):6110-5. [PubMed: 15071180] [MGI Ref ID J:89586]
Kather A; Chantakru S; He H; Minhas K; Foster R; Markert UR; Pfeffer K; Croy BA. 2003. Neither lymphotoxin alpha nor lymphotoxin beta receptor expression is required for biogenesis of lymphoid aggregates or differentiation of natural killer cells in the pregnant mouse uterus. Immunology 108(3):338-45. [PubMed: 12603600] [MGI Ref ID J:82424]
Moyron-Quiroz JE; Rangel-Moreno J; Kusser K; Hartson L; Sprague F; Goodrich S; Woodland DL; Lund FE; Randall TD. 2004. Role of inducible bronchus associated lymphoid tissue (iBALT) in respiratory immunity. Nat Med 10(9):927-34. [PubMed: 15311275] [MGI Ref ID J:92841]
Schreyer SA; Vick CM; LeBoeuf RC. 2002. Loss of Lymphotoxin-alpha but Not Tumor Necrosis Factor-alpha Reduces Atherosclerosis in Mice. J Biol Chem 277(14):12364-8. [PubMed: 11809756] [MGI Ref ID J:75788]
Ltatm1Dch relatedAbe K; Yarovinsky FO; Murakami T; Shakhov AN; Tumanov AV; Ito D; Drutskaya LN; Pfeffer K; Kuprash DV; Komschlies KL; Nedospasov SA. 2003. Distinct contributions of TNF and LT cytokines to the development of dendritic cells in vitro and their recruitment in vivo. Blood 101(4):1477-83. [PubMed: 12560241] [MGI Ref ID J:115537]
Alexopoulou L; Pasparakis M; Kollias G. 1998. Complementation of lymphotoxin alpha knockout mice with tumor necrosis factor-expressing transgenes rectifies defective splenic structure and function. J Exp Med 188(4):745-54. [PubMed: 9705956] [MGI Ref ID J:110953]
Alimzhanov MB; Kuprash DV; Kosco-Vilbois MH; Luz A; Turetskaya RL ; Tarakhovsky A ; Rajewsky K ; Nedospasov SA ; Pfeffer K. 1997. Abnormal development of secondary lymphoid tissues in lymphotoxin beta-deficient mice. Proc Natl Acad Sci U S A 94(17):9302-7. [PubMed: 9256477] [MGI Ref ID J:42508]
Anders RA; Subudhi SK; Wang J; Pfeffer K; Fu YX. 2005. Contribution of the lymphotoxin beta receptor to liver regeneration. J Immunol 175(2):1295-300. [PubMed: 16002734] [MGI Ref ID J:100756]
Ansel KM; Ngo VN; Hyman PL; Luther SA; Forster R; Sedgwick JD; Browning JL; Lipp M; Cyster JG. 2000. A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature 406(6793):309-14. [PubMed: 10917533] [MGI Ref ID J:78282]
Aya K; Alhawagri M; Hagen-Stapleton A; Kitaura H; Kanagawa O; Veis Novack D. 2005. NF-(kappa)B-inducing kinase controls lymphocyte and osteoclast activities in inflammatory arthritis. J Clin Invest 115(7):1848-54. [PubMed: 15937549] [MGI Ref ID J:99806]
Beilhack A; Schulz S; Baker J; Beilhack GF; Nishimura R; Baker EM; Landan G; Herman EI; Butcher EC; Contag CH; Negrin RS. 2008. Prevention of acute graft-versus-host disease by blocking T-cell entry to secondary lymphoid organs. Blood 111(5):2919-28. [PubMed: 17989315] [MGI Ref ID J:131612]
Berger DP; Naniche D; Crowley MT; Koni PA; Flavell RA; Oldstone MB. 1999. Lymphotoxin-beta-deficient mice show defective antiviral immunity. Virology 260(1):136-47. [PubMed: 10405365] [MGI Ref ID J:102625]
Bopst M; Garcia I; Guler R; Olleros ML; Rulicke T; Muller M; Wyss S; Frei K; Le Hir M; Eugster HP. 2001. Differential effects of TNF and LTalpha in the host defense against M. bovis BCG. Eur J Immunol 31(6):1935-43. [PubMed: 11433391] [MGI Ref ID J:115600]
Brown GR; Lee EL; El-Hayek J; Kintner K; Luck C. 2005. IL-12-independent LIGHT signaling enhances MHC class II disparate CD4+ T cell alloproliferation, IFN-gamma responses, and intestinal graft-versus-host disease. J Immunol 174(8):4688-95. [PubMed: 15814693] [MGI Ref ID J:109999]
Chin RK; Lo JC; Kim O; Blink SE; Christiansen PA; Peterson P; Wang Y; Ware C; Fu YX. 2003. Lymphotoxin pathway directs thymic Aire expression. Nat Immunol 4(11):1121-7. [PubMed: 14517552] [MGI Ref ID J:86259]
Chin RK; Zhu M; Christiansen PA; Liu W; Ware C; Peltonen L; Zhang X; Guo L; Han S; Zheng B; Fu YX. 2006. Lymphotoxin pathway-directed, autoimmune regulator-independent central tolerance to arthritogenic collagen. J Immunol 177(1):290-7. [PubMed: 16785524] [MGI Ref ID J:134431]
Ching S; He L; Lai W; Quan N. 2005. IL-1 type I receptor plays a key role in mediating the recruitment of leukocytes into the central nervous system. Brain Behav Immun 19(2):127-37. [PubMed: 15664785] [MGI Ref ID J:105351]
Cui CY; Hashimoto T; Grivennikov SI; Piao Y; Nedospasov SA; Schlessinger D. 2006. Ectodysplasin regulates the lymphotoxin-beta pathway for hair differentiation. Proc Natl Acad Sci U S A 103(24):9142-7. [PubMed: 16738056] [MGI Ref ID J:111051]
De Trez C; Schneider K; Potter K; Droin N; Fulton J; Norris PS; Ha SW; Fu YX; Murphy T; Murphy KM; Pfeffer K; Benedict CA; Ware CF. 2008. The inhibitory HVEM-BTLA pathway counter regulates lymphotoxin receptor signaling to achieve homeostasis of dendritic cells. J Immunol 180(1):238-48. [PubMed: 18097025] [MGI Ref ID J:130896]
Douni E; Kollias G. 1998. A critical role of the p75 tumor necrosis factor receptor (p75TNF-R) in organ inflammation independent of TNF, lymphotoxin alpha, or the p55TNF-R. J Exp Med 188(7):1343-52. [PubMed: 9763613] [MGI Ref ID J:114744]
Eberl G; Littman DR. 2004. Thymic origin of intestinal alphabeta T Cells Revealed by Fate Mapping of RORgammat+ Cells. Science 305(5681):248-51. [PubMed: 15247480] [MGI Ref ID J:91566]
Ehlers S; Holscher C; Scheu S; Tertilt C; Hehlgans T; Suwinski J; Endres R; Pfeffer K. 2003. The lymphotoxin beta receptor is critically involved in controlling infections with the intracellular pathogens Mycobacterium tuberculosis and Listeria monocytogenes. J Immunol 170(10):5210-8. [PubMed: 12734369] [MGI Ref ID J:109995]
Elewaut D; Brossay L; Santee SM; Naidenko OV; Burdin N; De Winter H; Matsuda J; Ware CF; Cheroutre H; Kronenberg M. 2000. Membrane lymphotoxin is required for the development of different subpopulations of NK T cells. J Immunol 165(2):671-9. [PubMed: 10878339] [MGI Ref ID J:106601]
Engwerda CR; Ato M; Stager S; Alexander CE; Stanley AC; Kaye PM. 2004. Distinct roles for lymphotoxin-alpha and tumor necrosis factor in the control of Leishmania donovani infection. Am J Pathol 165(6):2123-33. [PubMed: 15579454] [MGI Ref ID J:94946]
Ericsson A; Kotarsky K; Svensson M; Sigvardsson M; Agace W. 2006. Functional characterization of the CCL25 promoter in small intestinal epithelial cells suggests a regulatory role for caudal-related homeobox (Cdx) transcription factors. J Immunol 176(6):3642-51. [PubMed: 16517733] [MGI Ref ID J:129504]
Finke D; Acha-Orbea H; Mattis A; Lipp M; Kraehenbuhl J. 2002. CD4+CD3- cells induce Peyer's patch development: role of alpha4beta1 integrin activation by CXCR5. Immunity 17(3):363-73. [PubMed: 12354388] [MGI Ref ID J:111436]
Fu YX; Huang G; Matsumoto M; Molina H; Chaplin DD. 1997. Independent signals regulate development of primary and secondary follicle structure in spleen and mesenteric lymph node. Proc Natl Acad Sci U S A 94(11):5739-43. [PubMed: 9159143] [MGI Ref ID J:78647]
Fu YX; Huang G; Wang Y; Chaplin DD. 1998. B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin alpha-dependent fashion. J Exp Med 187(7):1009-18. [PubMed: 9529317] [MGI Ref ID J:118756]
Fu YX; Huang G; Wang Y; Chaplin DD. 2000. Lymphotoxin-alpha-dependent spleen microenvironment supports the generation of memory B cells and is required for their subsequent antigen-induced activation. J Immunol 164(5):2508-14. [PubMed: 10679088] [MGI Ref ID J:126988]
Fu YX; Molina H; Matsumoto M; Huang G; Min J; Chaplin DD. 1997. Lymphotoxin-alpha (LTalpha) supports development of splenic follicular structure that is required for IgG responses. J Exp Med 185(12):2111-20. [PubMed: 9182683] [MGI Ref ID J:41079]
Fukuyama S; Hiroi T; Yokota Y; Rennert PD; Yanagita M; Kinoshita N; Terawaki S; Shikina T; Yamamoto M; Kurono Y; Kiyono H. 2002. Initiation of NALT organogenesis is independent of the IL-7R, LTbetaR, and NIK signaling pathways but requires the Id2 gene and CD3(-)CD4(+)CD45(+) cells. Immunity 17(1):31-40. [PubMed: 12150889] [MGI Ref ID J:78101]
Furtado GC; Marinkovic T; Martin AP; Garin A; Hoch B; Hubner W; Chen BK; Genden E; Skobe M; Lira SA. 2007. Lymphotoxin beta receptor signaling is required for inflammatory lymphangiogenesis in the thyroid. Proc Natl Acad Sci U S A 104(12):5026-31. [PubMed: 17360402] [MGI Ref ID J:120088]
Futterer A; Mink K; Luz A; Kosco-Vilbois MH; Pfeffer K. 1998. The lymphotoxin beta receptor controls organogenesis and affinity maturation in peripheral lymphoid tissues. Immunity 9(1):59-70. [PubMed: 9697836] [MGI Ref ID J:48837]
Gajewska BU; Alvarez D; Vidric M; Goncharova S; Stampfli MR; Coyle AJ; Gutierrez-Ramos JC; Jordana M. 2001. Generation of experimental allergic airways inflammation in the absence of draining lymph nodes. J Clin Invest 108(4):577-83. [PubMed: 11518731] [MGI Ref ID J:118404]
Goluszko E; Hjelmstrom P; Deng C; Poussin MA; Ruddle NH; Christadoss P. 2001. Lymphotoxin-alpha deficiency completely protects C57BL/6 mice from developing clinical experimental autoimmune myasthenia gravis. J Neuroimmunol 113(1):109-18. [PubMed: 11137582] [MGI Ref ID J:102964]
Hamada H; Hiroi T; Nishiyama Y; Takahashi H; Masunaga Y; Hachimura S; Kaminogawa S; Takahashi-Iwanaga H; Iwanaga T; Kiyono H; Yamamoto H; Ishikawa H. 2002. Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine. J Immunol 168(1):57-64. [PubMed: 11751946] [MGI Ref ID J:73436]
Harmsen A; Kusser K; Hartson L; Tighe M; Sunshine MJ; Sedgwick JD; Choi Y; Littman DR; Randall TD. 2002. Cutting edge: organogenesis of nasal-associated lymphoid tissue (NALT) occurs independently of lymphotoxin-alpha (LT alpha) and retinoic acid receptor-related orphan receptor-gamma, but the organization of NALT is LT alpha dependent. J Immunol 168(3):986-90. [PubMed: 11801629] [MGI Ref ID J:111963]
Heikenwalder M; Prinz M; Zeller N; Lang KS; Junt T; Rossi S; Tumanov A; Schmidt H; Priller J; Flatz L; Rulicke T; Macpherson AJ; Hollander GA; Nedospasov SA; Aguzzi A. 2008. Overexpression of lymphotoxin in T cells induces fulminant thymic involution. Am J Pathol 172(6):1555-70. [PubMed: 18483211] [MGI Ref ID J:136357]
Heikenwalder M; Zeller N; Seeger H; Prinz M; Klohn PC; Schwarz P; Ruddle NH; Weissmann C; Aguzzi A. 2005. Chronic lymphocytic inflammation specifies the organ tropism of prions. Science 307(5712):1107-10. [PubMed: 15661974] [MGI Ref ID J:96344]
Iizuka K; Chaplin DD; Wang Y; Wu Q; Pegg LE; Yokoyama WM; Fu YX. 1999. Requirement for membrane lymphotoxin in natural killer cell development. Proc Natl Acad Sci U S A 96(11):6336-40. [PubMed: 10339588] [MGI Ref ID J:55470]
Ito D; Back TC; Shakhov AN; Wiltrout RH; Nedospasov SA. 1999. Mice with a targeted mutation in lymphotoxin-alpha exhibit enhanced tumor growth and metastasis: impaired NK cell development and recruitment. J Immunol 163(5):2809-15. [PubMed: 10453025] [MGI Ref ID J:57087]
Itoh M; Miyamoto K; Ooga T; Iwahashi K; Takeuchi Y. 1999. Spontaneous accumulation of eosinophils and macrophages throughout the stroma of the epididymis and vas deferens in alymphoplasia (aly) mutant mice: I. A histological study. Am J Reprod Immunol 42(4):246-53. [PubMed: 10580607] [MGI Ref ID J:59868]
Jang MH; Kweon MN; Iwatani K; Yamamoto M; Terahara K; Sasakawa C; Suzuki T; Nochi T; Yokota Y; Rennert PD; Hiroi T; Tamagawa H; Iijima H; Kunisawa J; Yuki Y; Kiyono H. 2004. Intestinal villous M cells: an antigen entry site in the mucosal epithelium. Proc Natl Acad Sci U S A 101(16):6110-5. [PubMed: 15071180] [MGI Ref ID J:89586]
Ji H; Pettit A; Ohmura K; Ortiz-Lopez A; Duchatelle V; Degott C; Gravallese E; Mathis D; Benoist C. 2002. Critical roles for interleukin 1 and tumor necrosis factor alpha in antibody-induced arthritis. J Exp Med 196(1):77-85. [PubMed: 12093872] [MGI Ref ID J:132920]
Kajiura F; Sun S; Nomura T; Izumi K; Ueno T; Bando Y; Kuroda N; Han H; Li Y; Matsushima A; Takahama Y; Sakaguchi S; Mitani T; Matsumoto M. 2004. NF-kappaB-inducing kinase establishes self-tolerance in a thymic stroma-dependent manner. J Immunol 172(4):2067-75. [PubMed: 14764671] [MGI Ref ID J:87993]
Kang HS; Blink SE; Chin RK; Lee Y; Kim O; Weinstock J; Waldschmidt T; Conrad D; Chen B; Solway J; Sperling AI; Fu YX. 2003. Lymphotoxin is required for maintaining physiological levels of serum IgE that minimizes Th1-mediated airway inflammation. J Exp Med 198(11):1643-52. [PubMed: 14638845] [MGI Ref ID J:86810]
Kang HS; Chin RK; Wang Y; Yu P; Wang J; Newell KA; Fu YX. 2002. Signaling via LTbetaR on the lamina propria stromal cells of the gut is required for IgA production. Nat Immunol 3(6):576-82. [PubMed: 12006975] [MGI Ref ID J:76773]
Kather A; Chantakru S; He H; Minhas K; Foster R; Markert UR; Pfeffer K; Croy BA. 2003. Neither lymphotoxin alpha nor lymphotoxin beta receptor expression is required for biogenesis of lymphoid aggregates or differentiation of natural killer cells in the pregnant mouse uterus. Immunology 108(3):338-45. [PubMed: 12603600] [MGI Ref ID J:82424]
Klonowski KD; Marzo AL; Williams KJ; Lee SJ; Pham QM; Lefrancois L. 2006. CD8 T cell recall responses are regulated by the tissue tropism of the memory cell and pathogen. J Immunol 177(10):6738-46. [PubMed: 17082587] [MGI Ref ID J:140494]
Koni PA; Flavell RA. 1998. A role for tumor necrosis factor receptor type 1 in gut-associated lymphoid tissue development: genetic evidence of synergism with lymphotoxin beta. J Exp Med 187(12):1977-83. [PubMed: 9625757] [MGI Ref ID J:110881]
Koni PA; Sacca R; Lawton P; Browning JL; Ruddle NH; Flavell RA. 1997. Distinct roles in lymphoid organogenesis for lymphotoxins alpha and beta revealed in lymphotoxin beta-deficient mice. Immunity 6(4):491-500. [PubMed: 9133428] [MGI Ref ID J:39746]
Kranich J; Krautler NJ; Heinen E; Polymenidou M; Bridel C; Schildknecht A; Huber C; Kosco-Vilbois MH; Zinkernagel R; Miele G; Aguzzi A. 2008. Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8. J Exp Med 205(6):1293-302. [PubMed: 18490487] [MGI Ref ID J:137077]
Kuprash DV; Alimzhanov MB; Tumanov AV; Anderson AO; Pfeffer K; Nedospasov SA. 1999. TNF and lymphotoxin beta cooperate in the maintenance of secondary lymphoid tissue microarchitecture but not in the development of lymph nodes. J Immunol 163(12):6575-80. [PubMed: 10586051] [MGI Ref ID J:58981]
Kuprash DV; Alimzhanov MB; Tumanov AV; Grivennikov SI; Shakhov AN; Drutskaya LN; Marino MW; Turetskaya RL; Anderson AO; Rajewsky K; Pfeffer K; Nedospasov SA. 2002. Redundancy in tumor necrosis factor (TNF) and lymphotoxin (LT) signaling in vivo: mice with inactivation of the entire TNF/LT locus versus single-knockout mice. Mol Cell Biol 22(24):8626-34. [PubMed: 12446781] [MGI Ref ID J:80616]
Kwa SF; Beverley P; Smith AL. 2006. Peyer's patches are required for the induction of rapid Th1 responses in the gut and mesenteric lymph nodes during an enteric infection. J Immunol 176(12):7533-41. [PubMed: 16751400] [MGI Ref ID J:132354]
Laouar A; Haridas V; Vargas D; Zhinan X; Chaplin D; van Lier RA; Manjunath N. 2005. CD70+ antigen-presenting cells control the proliferation and differentiation of T cells in the intestinal mucosa. Nat Immunol 6(7):698-706. [PubMed: 15937486] [MGI Ref ID J:99148]
Liepinsh DJ; Grivennikov SI; Klarmann KD; Lagarkova MA; Drutskaya MS; Lockett SJ; Tessarollo L; McAuliffe M; Keller JR; Kuprash DV; Nedospasov SA. 2006. Novel lymphotoxin alpha (LTalpha) knockout mice with unperturbed tumor necrosis factor expression: reassessing LTalpha biological functions. Mol Cell Biol 26(11):4214-25. [PubMed: 16705172] [MGI Ref ID J:109621]
Lin X; Ma X; Rodriguez M; Feng X; Zoecklein L; Fu YX; Roos RP. 2003. Membrane lymphotoxin is required for resistance to Theiler's virus infection. Int Immunol 15(8):955-62. [PubMed: 12882833] [MGI Ref ID J:84938]
Lo JC; Chin RK; Lee Y; Kang HS; Wang Y; Weinstock JV; Banks T; Ware CF; Franzoso G; Fu YX. 2003. Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues. J Clin Invest 112(10):1495-505. [PubMed: 14617751] [MGI Ref ID J:113397]
Lo JC; Wang Y; Tumanov AV; Bamji M; Yao Z; Reardon CA; Getz GS; Fu YX. 2007. Lymphotoxin beta receptor-dependent control of lipid homeostasis. Science 316(5822):285-8. [PubMed: 17431181] [MGI Ref ID J:120962]
Lorenz RG; Chaplin DD; McDonald KG; McDonough JS; Newberry RD. 2003. Isolated Lymphoid Follicle Formation Is Inducible and Dependent Upon Lymphotoxin-Sufficient B Lymphocytes, Lymphotoxin beta Receptor, and TNF Receptor I Function. J Immunol 170(11):5475-82. [PubMed: 12759424] [MGI Ref ID J:83455]
Lund FE; Partida-Sanchez S; Lee BO; Kusser KL; Hartson L; Hogan RJ; Woodland DL; Randall TD. 2002. Lymphotoxin-alpha-Deficient Mice Make Delayed, But Effective, T and B Cell Responses to Influenza. J Immunol 169(9):5236-43. [PubMed: 12391242] [MGI Ref ID J:79758]
Luther SA; Lopez T; Bai W; Hanahan D; Cyster JG. 2000. BLC expression in pancreatic islets causes B cell recruitment and lymphotoxin-dependent lymphoid neogenesis. Immunity 12(5):471-81. [PubMed: 10843380] [MGI Ref ID J:110548]
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Mandik-Nayak L; Huang G; Sheehan KC; Erikson J; Chaplin DD. 2001. Signaling Through TNF Receptor p55 in TNF-alpha-Deficient Mice Alters the CXCL13/CCL19/CCL21 Ratio in the Spleen and Induces Maturation and Migration of Anergic B Cells into the B Cell Follicle. J Immunol 167(4):1920-8. [PubMed: 11489971] [MGI Ref ID J:70819]
Mariathasan S; Matsumoto M; Baranyay F; Nahm MH; Kanagawa O; Chaplin DD. 1995. Absence of lymph nodes in lymphotoxin-alpha(LT alpha)-deficient mice is due to abnormal organ development, not defective lymphocyte migration. J Inflamm 45(1):72-8. [PubMed: 7583355] [MGI Ref ID J:29136]
Matsumoto M; Fu YX; Molina H; Chaplin DD. 1997. Lymphotoxin-alpha-deficient and TNF receptor-I-deficient mice define developmental and functional characteristics of germinal centers. Immunol Rev 156:137-44. [PubMed: 9176705] [MGI Ref ID J:40209]
Matsumoto M; Fu YX; Molina H; Huang G; Kim J; Thomas DA; Nahm MH; Chaplin DD. 1997. Distinct roles of lymphotoxin alpha and the type I tumor necrosis factor (TNF) receptor in the establishment of follicular dendritic cells from non-bone marrow-derived cells. J Exp Med 186(12):1997-2004. [PubMed: 9396768] [MGI Ref ID J:44877]
Matsumoto M; Iwamasa K; Rennert PD; Yamada T; Suzuki R; Matsushima A ; Okabe M ; Fujita S ; Yokoyama M. 1999. Involvement of distinct cellular compartments in the abnormal lymphoid organogenesis in lymphotoxin-alpha-deficient mice and alymphoplasia (aly) mice defined by the chimeric analysis. J Immunol 163(3):1584-91. [PubMed: 10415063] [MGI Ref ID J:56996]
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McDonald KG; McDonough JS; Wang C; Kucharzik T; Williams IR; Newberry RD. 2007. CC chemokine receptor 6 expression by B lymphocytes is essential for the development of isolated lymphoid follicles. Am J Pathol 170(4):1229-40. [PubMed: 17392163] [MGI Ref ID J:120122]
Meier D; Bornmann C; Chappaz S; Schmutz S; Otten LA; Ceredig R; Acha-Orbea H; Finke D. 2007. Ectopic lymphoid-organ development occurs through interleukin 7-mediated enhanced survival of lymphoid-tissue-inducer cells. Immunity 26(5):643-54. [PubMed: 17521585] [MGI Ref ID J:123554]
Mendez-Fernandez YV; Hansen MJ; Rodriguez M; Pease LR. 2005. Anatomical and cellular requirements for the activation and migration of virus-specific CD8+ T cells to the brain during Theiler's virus infection. J Virol 79(5):3063-70. [PubMed: 15709026] [MGI Ref ID J:96694]
Moyron-Quiroz JE; Rangel-Moreno J; Kusser K; Hartson L; Sprague F; Goodrich S; Woodland DL; Lund FE; Randall TD. 2004. Role of inducible bronchus associated lymphoid tissue (iBALT) in respiratory immunity. Nat Med 10(9):927-34. [PubMed: 15311275] [MGI Ref ID J:92841]
Newberry RD; McDonough JS; McDonald KG; Lorenz RG. 2002. Postgestational lymphotoxin/lymphotoxin beta receptor interactions are essential for the presence of intestinal B lymphocytes. J Immunol 168(10):4988-97. [PubMed: 11994450] [MGI Ref ID J:127286]
Ngo VN; Cornall RJ; Cyster JG. 2001. Splenic T zone development is B cell dependent. J Exp Med 194(11):1649-60. [PubMed: 11733579] [MGI Ref ID J:73100]
Nolte MA; Arens R; Kraus M; van Oers MH; Kraal G; van Lier RA; Mebius RE. 2004. B cells are crucial for both development and maintenance of the splenic marginal zone. J Immunol 172(6):3620-7. [PubMed: 15004164] [MGI Ref ID J:88620]
Oldstone MB; Race R; Thomas D; Lewicki H; Homann D; Smelt S; Holz A; Koni P; Lo D; Chesebro B; Flavell R. 2002. Lymphotoxin-alpha- and lymphotoxin-beta-deficient mice differ in susceptibility to scrapie: evidence against dendritic cell involvement in neuroinvasion. J Virol 76(9):4357-63. [PubMed: 11932402] [MGI Ref ID J:126282]
Olleros ML; Guler R; Corazza N; Vesin D; Eugster HP; Marchal G; Chavarot P; Mueller C; Garcia I. 2002. Transmembrane TNF induces an efficient cell-mediated immunity and resistance to Mycobacterium bovis bacillus Calmette-Guerin infection in the absence of secreted TNF and lymphotoxin-alpha. J Immunol 168(7):3394-401. [PubMed: 11907097] [MGI Ref ID J:75580]
Pasparakis M; Kousteni S; Peschon J; Kollias G. 2000. Tumor necrosis factor and the p55TNF receptor are required for optimal development of the marginal sinus and for migration of follicular dendritic cell precursors into splenic follicles. Cell Immunol 201(1):33-41. [PubMed: 10805971] [MGI Ref ID J:62236]
Plant SR; Arnett HA; Ting JP. 2005. Astroglial-derived lymphotoxin-alpha exacerbates inflammation and demyelination, but not remyelination. Glia 49(1):1-14. [PubMed: 15382206] [MGI Ref ID J:104812]
Rangel-Moreno J; Moyron-Quiroz J; Kusser K; Hartson L; Nakano H; Randall TD. 2005. Role of CXC chemokine ligand 13, CC chemokine ligand (CCL) 19, and CCL21 in the organization and function of nasal-associated lymphoid tissue. J Immunol 175(8):4904-13. [PubMed: 16210592] [MGI Ref ID J:119053]
Rebholz B; Haase I; Eckelt B; Paxian S; Flaig MJ; Ghoreschi K; Nedospasov SA; Mailhammer R; Debey-Pascher S; Schultze JL; Weindl G; Forster I; Huss R; Stratis A; Ruzicka T; Rocken M; Pfeffer K; Schmid RM; Rupec RA. 2007. Crosstalk between Keratinocytes and Adaptive Immune Cells in an IkappaBalpha Protein-Mediated Inflammatory Disease of the Skin. Immunity 27(2):296-307. [PubMed: 17692539] [MGI Ref ID J:124343]
Rennert PD; James D; Mackay F; Browning JL; Hochman PS. 1998. Lymph node genesis is induced by signaling through the lymphotoxin beta receptor. Immunity 9(1):71-9. [PubMed: 9697837] [MGI Ref ID J:48789]
Sacca R; Turley S; Soong L; Mellman I; Ruddle NH. 1997. Transgenic expression of lymphotoxin restores lymph nodes to lymphotoxin-alpha-deficient mice. J Immunol 159(9):4252-60. [PubMed: 9379020] [MGI Ref ID J:110671]
Sedger LM; Hou S; Osvath SR; Glaccum MB; Peschon JJ; van Rooijen N; Hyland L. 2002. Bone marrow B cell apoptosis during in vivo influenza virus infection requires TNF-alpha and lymphotoxin-alpha. J Immunol 169(11):6193-201. [PubMed: 12444124] [MGI Ref ID J:118778]
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Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain is maintained by homozygous sibling matings. Expected coat color from breeding:Black Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $87.00 Female or Male Homozygous for Ltatm1Dch *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $174.00 Homozygous for Ltatm1Dch x Homozygous for Ltatm1Dch
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $113.10 Female or Male Homozygous for Ltatm1Dch *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $226.20 Homozygous for Ltatm1Dch x Homozygous for Ltatm1Dch
| Supply Notes |
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| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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