Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Rudolf Jaenisch,   Massachusetts Institute of Technology

Appearance
black, white-bellied agouti, or albino
Related Genotype: segregating for a, A^w, and Tyr^c

Description
Mice heterozygous for the Bdnf^tm1Jae mutation show about 1/2 normal levels of Bdnf mRNA, but appear normal. Mice homozygous for the Bdnf^tm1Jae mutation are smaller than normal siblings and most die within the second postnatal week. They have defective coordination of movements and balance. They also exhibit head bobbing and spinning during periods of hyperactivity. There are rare survivors to adulthood. There is excessive degeneration in all sensory ganglia examined, including the vestibular ganglion. Motor neurons are not affected. BDNF can prevent death of central motor neurons and other neurons in vitro, but does not affect these in vivo.

Development
The Bdnf^tm1Jae mutant strain was developed in the laboratory of Dr. Rudolf Jaenisch at the Whitehead Institute for Biomedical Research at the Massachusetts Institute of Technology. The 129-derived J1 ES cell line was used.

Control Information

	Control
	Wild-type from the colony
		This strain is a mixture of 129, BALB/c, and C57BL/6 inbred strains. Wildtype mice from the colony may be used as controls.
Considerations for Choosing Controls

Related Strains

Strains carrying   Bdnf^tm1Jae allele

002266

B6.129S4-Bdnftm1Jae/J

View Strains carrying   Bdnf^tm1Jae     (1 strain)

Strains carrying other alleles of Bdnf

004339

STOCK Bdnftm3Jae/J

View Strains carrying other alleles of Bdnf     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Bdnf^tm1Jae/Bdnf^tm1Jae

        involves: 129S4/SvJae

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:17123)
  • most homozygotes die in the second postnatal week, with rare survivors achieving adult stages
• behavior/neurological phenotype
• hyperactivity (MGI Ref ID J:17123)
• impaired balance (MGI Ref ID J:17123)
• circling (MGI Ref ID J:17123)
• head bobbing (MGI Ref ID J:17123)
• head tilt (MGI Ref ID J:17123)
• impaired limb coordination (MGI Ref ID J:17123)
• spinning (MGI Ref ID J:17123)
• growth/size phenotype
• decreased body size (MGI Ref ID J:17123)
  • mice are reduced in size compared to control littermates
• nervous system phenotype
• *normal* nervous system phenotype (MGI Ref ID J:17123)
  • no differences are seen in the morphology of the central and peripheral nerve processes from the trigeminal, geniculate, petrose and nodose ganglia at E10.5-11.5
• abnormal trigeminal nerve morphology (MGI Ref ID J:123022)
  • mice exhibit a 43% loss of trigeminal neurons at birth
• abnormal type I vestibular cell (MGI Ref ID J:17123)
  • no nerve chalicies are found associated with type I hair cells of the vestibule
• abnormal vestibular nerve morphology (MGI Ref ID J:17123)
  • innervation of the vestibular compartment of the inner ear is severely compromised
  • only small fiber bundles enter the saccule, utricle and ampulla of the semicircular ducts compared to controls; the remaining fibers terminate in the connective tissue adjacent to the sensory epithelia of the saccule, utricle and cristae of the semicurcular ducts
  • no nerve chalicies are found associated with type I hair cells of the vestibule
• decreased sensory neuron number (MGI Ref ID J:25566)
  • significant loss of all sensory neuron types in the nodose-petrosal, geniculate and vestibular ganglia in homozygous mice
  • no difference in the number of facial motor neurons is seen compared to controls
  • the numbers of neurons in many sensory ganglia are reduced compared to controls
  • the number of facial motor neurons and sympathetic superior cervical ganglion neurons is not different from controls
• increased neuron apoptosis (MGI Ref ID J:123022)
  • at E12 and E14, neuron apoptosis is increased 143% and 155%, respectively, compared to in wild-type mice
• small L4 dorsal root ganglion (MGI Ref ID J:17123)
  • approximately 30% of the neurons are lost from the L4 DRG at P14-16
• small geniculate ganglion (MGI Ref ID J:25566)
  • approximately half of the neurons are lost from the geniculate ganglion at E18.5
• small mesencephalic trigeminal nucleus (MGI Ref ID J:17123)
  • approximately half of the neurons are lost from the mesencephalic trigeminal nucleus at P14-16
• small nodose ganglion (MGI Ref ID J:25566)
  • greater than half of the neurons are lost from the nodose-petrosal ganglion at E18.5
  • approximately 65% of the neurons are lost from the nodose ganglion at P14-16
• small petrosal ganglion (MGI Ref ID J:25566)
  • greater than half of the neurons are lost from the nodose-petrosal ganglion at E18.5
• small trigeminal ganglion (MGI Ref ID J:17123)
  • approximately half of the neurons are lost from the trigeminal ganglion at P14-16
• small vestibular ganglion (MGI Ref ID J:25566)
  • approximately 75% of the neurons are lost from the vestibular ganglion at E18.5
  • approximately 80% of the neurons are lost from the vestibular ganglion at P14-16
  • vestibular neurons appear atrophic
• hearing/vestibular/ear phenotype
• *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:17123)
  • despite abnormal innervation of the vestibular compartment, the structural integrity of the organ of Corti, macula of the saccula and utricle, and the cristae of the semicircular ducts appear similar to controls
  • innervation of the cochlear inner and outer hair cells appears similar to controls
  • mice respond to auditory stimuli
• abnormal type I vestibular cell (MGI Ref ID J:17123)
  • no nerve chalicies are found associated with type I hair cells of the vestibule
• circling (MGI Ref ID J:17123)
• head bobbing (MGI Ref ID J:17123)
• head tilt (MGI Ref ID J:17123)

Bdnf^tm1Jae/Bdnf^tm1Jae

        B6.129S4-Bdnf^tm1Jae

• taste/olfaction phenotype
• abnormal gustatory papillae taste buds (MGI Ref ID J:90280)
  • at P7 homozygous mice have 79% fewer vallate taste buds than wild-type mice
• nervous system phenotype
• abnormal gustatory papillae taste buds (MGI Ref ID J:90280)
  • at P7 homozygous mice have 79% fewer vallate taste buds than wild-type mice
• abnormal sensory neuron innervation (MGI Ref ID J:90280)
  • innervation of the fungiform papillae is reduced with a shortfall in the geniculate ganglion neurons that supply taste neurons to the fungiform papillae

Bdnf^tm1Jae/Bdnf^tm1Jae

        involves: 129S4/SvJae * BALB/c

• nervous system phenotype
• abnormal neuron morphology (MGI Ref ID J:46125)
  • mice exhibit an increased in sympathetic neuron number (21 318+/-1 627 compared to 15 690+/-617 in wild-type mice) and size
• abnormal neuron apoptosis (MGI Ref ID J:46125)
  • sympathetic neuron apoptosis is developmentally delayed
  • cell death in culture following the withdrawal of NGF is delayed
• increased neuron number (MGI Ref ID J:46125)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Bdnf^tm1Jae related

Apoptosis Research
Extracellular Modulators

Neurobiology Research
Neurotrophic Factor Defects
Vestibular and Hearing Defects

Sensorineural Research
Vestibular and Hearing Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Bdnftm1Jae
Allele Name		targeted mutation 1, Rudolf Jaenisch
Allele Type		Targeted (knock-out)
Common Name(s)		BDNF-;
Mutation Made By		Rudolf Jaenisch,   Whitehead Institute (MIT)
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Bdnf, brain derived neurotrophic factor
Chromosome		2
Gene Common Name(s)		MGC105254; MGC34632;
Molecular Note		A part of exon 5 of the gene was deleted and replaced by a neomycin cassette. This deletion left 49 and 30 amino acids in the N and C termini, respectively. Northern blot analysis on RNA derived from cerebral cortex showed that no detectable transcriptis expressed from this allele. [MGI Ref ID J:17123]

Genotyping

Genotyping Information

Genotyping Protocols

Bdnf^tm1Jae, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Ernfors P; Lee KF; Jaenisch R. 1994. Mice lacking brain-derived neurotrophic factor develop with sensory deficits. Nature 368(6467):147-50. [PubMed: 8139657]  [MGI Ref ID J:17123]

Additional References

Fan L; Girnius S; Oakley B. 2004. Support of trigeminal sensory neurons by nonneuronal p75 neurotrophin receptors. Brain Res Dev Brain Res 150(1):23-39. [PubMed: 15126035]  [MGI Ref ID J:90280]

Ward NL; Hagg T. 2000. BDNF is needed for postnatal maturation of basal forebrain and neostriatum cholinergic neurons in vivo. Exp Neurol 162(2):297-310. [PubMed: 10739636]  [MGI Ref ID J:61704]

Bdnf^tm1Jae related

Agerman K; Baudet C; Fundin B; Willson C; Ernfors P. 2000. Attenuation of a caspase-3 dependent cell death in NT4- and p75-deficient embryonic sensory neurons. Mol Cell Neurosci 16(3):258-68. [PubMed: 10995552]  [MGI Ref ID J:123022]

Alkhamrah BA; Hoshino N; Kawano Y; Harada F; Hanada K; Maeda T. 2003. The periodontal Ruffini endings in brain derived neurotrophic factor (BDNF) deficient mice. Arch Histol Cytol 66(1):73-81. [PubMed: 12703556]  [MGI Ref ID J:83509]

Asztely F; Kokaia M; Olofsdotter K; Ortegren U; Lindvall O. 2000. Afferent-specific modulation of short-term synaptic plasticity by neurotrophins in dentate gyrus. Eur J Neurosci 12(2):662-9. [PubMed: 10712646]  [MGI Ref ID J:108125]

Baker SA; Stanford LE; Brown RE; Hagg T. 2005. Maturation but not survival of dopaminergic nigrostriatal neurons is affected in developing and aging BDNF-deficient mice. Brain Res 1039(1-2):177-88. [PubMed: 15781060]  [MGI Ref ID J:97459]

Bamji SX; Majdan M; Pozniak CD; Belliveau DJ; Aloyz R; Kohn J; Causing CG; Miller FD. 1998. The p75 neurotrophin receptor mediates neuronal apoptosis and is essential for naturally occurring sympathetic neuron death. J Cell Biol 140(4):911-23. [PubMed: 9472042]  [MGI Ref ID J:46125]

Barco A; Patterson S; Alarcon JM; Gromova P; Mata-Roig M; Morozov A; Kandel ER. 2005. Gene expression profiling of facilitated L-LTP in VP16-CREB mice reveals that BDNF is critical for the maintenance of LTP and its synaptic capture. Neuron 48(1):123-37. [PubMed: 16202713]  [MGI Ref ID J:105355]

Barton ME; Shannon HE. 2005. The seizure-related phenotype of brain-derived neurotrophic factor knockdown mice. Neuroscience 136(2):563-9. [PubMed: 16198489]  [MGI Ref ID J:104591]

Bath KG; Mandairon N; Jing D; Rajagopal R; Kapoor R; Chen ZY; Khan T; Proenca CC; Kraemer R; Cleland TA; Hempstead BL; Chao MV; Lee FS. 2008. Variant brain-derived neurotrophic factor (Val66Met) alters adult olfactory bulb neurogenesis and spontaneous olfactory discrimination. J Neurosci 28(10):2383-93. [PubMed: 18322085]  [MGI Ref ID J:132776]

Borghesani PR; Peyrin JM; Klein R; Rubin J; Carter AR; Schwartz PM; Luster A; Corfas G; Segal RA. 2002. BDNF stimulates migration of cerebellar granule cells. Development 129(6):1435-42. [PubMed: 11880352]  [MGI Ref ID J:75046]

Canals JM; Pineda JR; Torres-Peraza JF; Bosch M; Martin-Ibanez R; Munoz MT; Mengod G; Ernfors P; Alberch J. 2004. Brain-derived neurotrophic factor regulates the onset and severity of motor dysfunction associated with enkephalinergic neuronal degeneration in Huntington's disease. J Neurosci 24(35):7727-39. [PubMed: 15342740]  [MGI Ref ID J:92634]

Cao L; Dhilla A; Mukai J; Blazeski R; Lodovichi C; Mason CA; Gogos JA. 2007. Genetic modulation of BDNF signaling affects the outcome of axonal competition in vivo. Curr Biol 17(11):911-21. [PubMed: 17493809]  [MGI Ref ID J:124050]

Carter AR; Berry EM; Segal RA. 2003. Regional expression of p75NTR contributes to neurotrophin regulation of cerebellar patterning. Mol Cell Neurosci 22(1):1-13. [PubMed: 12595234]  [MGI Ref ID J:82192]

Carter AR; Chen C; Schwartz PM; Segal RA. 2002. Brain-derived neurotrophic factor modulates cerebellar plasticity and synaptic ultrastructure. J Neurosci 22(4):1316-27. [PubMed: 11850459]  [MGI Ref ID J:74586]

Causing CG; Gloster A; Aloyz R; Bamji SX; Chang E; Fawcett J; Kuchel G; Miller FD. 1997. Synaptic innervation density is regulated by neuron-derived BDNF. Neuron 18(2):257-67. [PubMed: 9052796]  [MGI Ref ID J:127889]

Chourbaji S; Brandwein C; Vogt MA; Dormann C; Hellweg R; Gass P. 2008. Nature vs. nurture: Can enrichment rescue the behavioural phenotype of BDNF heterozygous mice? Behav Brain Res 192(2):254-8. [PubMed: 18538870]  [MGI Ref ID J:137632]

Chourbaji S; Hellweg R; Brandis D; Zorner B; Zacher C; Lang UE; Henn FA; Hortnagl H; Gass P. 2004. Mice with reduced brain-derived neurotrophic factor expression show decreased choline acetyltransferase activity, but regular brain monoamine levels and unaltered emotional behavior. Brain Res Mol Brain Res 121(1-2):28-36. [PubMed: 14969734]  [MGI Ref ID J:88037]

Cooper D; Oakley B. 1998. Functional redundancy and gustatory development in bdnf null mutant mice. Brain Res Dev Brain Res 105(1):79-84. [PubMed: 9497082]  [MGI Ref ID J:45553]

Daws LC; Munn JL; Valdez MF; Frosto-Burke T; Hensler JG. 2007. Serotonin transporter function, but not expression, is dependent on brain-derived neurotrophic factor (BDNF): in vivo studies in BDNF-deficient mice. J Neurochem 101(3):641-51. [PubMed: 17254018]  [MGI Ref ID J:121235]

Djalali S; Holtje M; Grosse G; Rothe T; Stroh T; Grosse J; Deng DR; Hellweg R; Grantyn R; Hortnagl H; Ahnert-Hilger G. 2005. Effects of brain-derived neurotrophic factor (BDNF) on glial cells and serotonergic neurones during development. J Neurochem 92(3):616-27. [PubMed: 15659231]  [MGI Ref ID J:96205]

Dluzen DE. 2004. The effect of gender and the neurotrophin, BDNF, upon methamphetamine-induced neurotoxicity of the nigrostriatal dopaminergic system in mice. Neurosci Lett 359(3):135-8. [PubMed: 15050682]  [MGI Ref ID J:131485]

Dluzen DE; Gao X; Story GM; Anderson LI; Kucera J; Walro JM. 2001. Evaluation of nigrostriatal dopaminergic function in adult +/+ and +/- BDNF mutant mice. Exp Neurol 170(1):121-8. [PubMed: 11421589]  [MGI Ref ID J:70481]

Dluzen DE; McDermott JL; Anderson LI; Kucera J; Joyce JN; Osredkar T; Walro JM. 2004. Age-related changes in nigrostriatal dopaminergic function are accentuated in +/- brain-derived neurotrophic factor mice. Neuroscience 128(1):201-8. [PubMed: 15450367]  [MGI Ref ID J:93611]

Dluzen DE; Story GM; Xu K; Kucera J; Walro JM. 1999. Alterations in nigrostriatal dopaminergic function within BDNF mutant mice. Exp Neurol 160(2):500-7. [PubMed: 10619567]  [MGI Ref ID J:58936]

Donovan MJ; Lin MI; Wiegn P; Ringstedt T; Kraemer R; Hahn R; Wang S; Ibanez CF; Rafii S; Hempstead BL. 2000. Brain derived neurotrophic factor is an endothelial cell survival factor required for intramyocardial vessel stabilization Development 127(21):4531-40. [PubMed: 11023857]  [MGI Ref ID J:64681]

Duman CH; Schlesinger L; Russell DS; Duman RS. 2008. Voluntary exercise produces antidepressant and anxiolytic behavioral effects in mice. Brain Res 1199:148-58. [PubMed: 18267317]  [MGI Ref ID J:133331]

ElShamy WM; Ernfors P. 1997. Brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 complement and cooperate with each other sequentially during visceral neuron development. J Neurosci 17(22):8667-75. [PubMed: 9348335]  [MGI Ref ID J:44086]

Endres M; Fan G; Hirt L; Fujii M; Matsushita K; Liu X; Jaenisch R; Moskowitz MA. 2000. Ischemic brain damage in mice after selectively modifying BDNF or NT4 gene expression. J Cereb Blood Flow Metab 20(1):139-44. [PubMed: 10616802]  [MGI Ref ID J:60087]

English AW; Meador W; Carrasco DI. 2005. Neurotrophin-4/5 is required for the early growth of regenerating axons in peripheral nerves. Eur J Neurosci 21(10):2624-34. [PubMed: 15926911]  [MGI Ref ID J:101068]

Ernfors P; Van De Water T; Loring J; Jaenisch R. 1995. Complementary roles of BDNF and NT-3 in vestibular and auditory development. Neuron 14(6):1153-64. [PubMed: 7605630]  [MGI Ref ID J:26749]

Fan G; Copray S; Huang EJ; Jones K; Yan Q; Walro J; Jaenisch R; Kucera J. 2000. Formation of a full complement of cranial proprioceptors requires multiple neurotrophins. Dev Dyn 218(2):359-70. [PubMed: 10842362]  [MGI Ref ID J:62766]

Fan L; Girnius S; Oakley B. 2004. Support of trigeminal sensory neurons by nonneuronal p75 neurotrophin receptors. Brain Res Dev Brain Res 150(1):23-39. [PubMed: 15126035]  [MGI Ref ID J:90280]

Fox EA; Byerly MS. 2004. A mechanism underlying mature-onset obesity: evidence from the hyperphagic phenotype of brain-derived neurotrophic factor mutants. Am J Physiol Regul Integr Comp Physiol 286(6):R994-1004. [PubMed: 15142855]  [MGI Ref ID J:109376]

Fundin BT; Silos-Santiago I; Ernfors P; Fagan AM; Aldskogius H ; DeChiara TM ; Phillips HS ; Barbacid M ; Yancopoulos GD ; Rice FL. 1997. Differential dependency of cutaneous mechanoreceptors on neurotrophins, trk receptors, and P75 LNGFR. Dev Biol 190(1):94-116. [PubMed: 9331334]  [MGI Ref ID J:43425]

Gacek RR; Khetarpal U. 1998. Neurotrophin 3, not brain-derived neurotrophic factor or neurotrophin 4, knockout mice have delay in vestibular compensation after unilateral labyrinthectomy. Laryngoscope 108(5):671-8. [PubMed: 9591544]  [MGI Ref ID J:113175]

Genoud C; Knott GW; Sakata K; Lu B; Welker E. 2004. Altered synapse formation in the adult somatosensory cortex of brain-derived neurotrophic factor heterozygote mice. J Neurosci 24(10):2394-400. [PubMed: 15014114]  [MGI Ref ID J:97009]

Gines S; Bosch M; Marco S; Gavalda N; Diaz-Hernandez M; Lucas JJ; Canals JM; Alberch J. 2006. Reduced expression of the TrkB receptor in Huntington's disease mouse models and in human brain. Eur J Neurosci 23(3):649-58. [PubMed: 16487146]  [MGI Ref ID J:107146]

Grosse G; Djalali S; Deng DR; Holtje M; Hinz B; Schwartzkopff K; Cygon M; Rothe T; Stroh T; Hellweg R; Ahnert-Hilger G; Hortnag H. 2005. Area-specific effects of brain-derived neurotrophic factor (BDNF) genetic ablation on various neuronal subtypes of the mouse brain. Brain Res Dev Brain Res 156(2):111-26. [PubMed: 16099299]  [MGI Ref ID J:99374]

Hall FS; Drgonova J; Goeb M; Uhl GR. 2003. Reduced behavioral effects of cocaine in heterozygous brain-derived neurotrophic factor (BDNF) knockout mice. Neuropsychopharmacology 28(8):1485-90. [PubMed: 12784114]  [MGI Ref ID J:106206]

Harada C; Harada T; Quah HM; Namekata K; Yoshida K; Ohno S; Tanaka K; Parada LF. 2005. Role of neurotrophin-4/5 in neural cell death during retinal development and ischemic retinal injury in vivo. Invest Ophthalmol Vis Sci 46(2):669-73. [PubMed: 15671298]  [MGI Ref ID J:104963]

Henneberger C; Juttner R; Rothe T; Grantyn R. 2002. Postsynaptic action of BDNF on GABAergic synaptic transmission in the superficial layers of the mouse superior colliculus. J Neurophysiol 88(2):595-603. [PubMed: 12163512]  [MGI Ref ID J:103288]

Henneberger C; Juttner R; Schmidt SA; Walter J; Meier JC; Rothe T; Grantyn R. 2005. GluR- and TrkB-mediated maturation of GABA receptor function during the period of eye opening. Eur J Neurosci 21(2):431-40. [PubMed: 15673442]  [MGI Ref ID J:100813]

Hoshino N; Harada F; Alkhamrah BA; Aita M; Kawano Y; Hanada K; Maeda T. 2003. Involvement of brain-derived neurotrophic factor (BDNF) in the development of periodontal Ruffini endings. Anat Rec A Discov Mol Cell Evol Biol 274(1):807-16. [PubMed: 12923891]  [MGI Ref ID J:106012]

Huang YZ; Pan E; Xiong ZQ; McNamara JO. 2008. Zinc-mediated transactivation of TrkB potentiates the hippocampal mossy fiber-CA3 pyramid synapse. Neuron 57(4):546-58. [PubMed: 18304484]  [MGI Ref ID J:135687]

Jahed A; Kawaja MD. 2005. The influences of p75 neurotrophin receptor and brain-derived neurotrophic factor in the sympathetic innervation of target tissues during murine postnatal development. Auton Neurosci 118(1-2):32-42. [PubMed: 15795176]  [MGI Ref ID J:101883]

Jourdi H; Iwakura Y; Narisawa-Saito M; Ibaraki K; Xiong H; Watanabe M; Hayashi Y; Takei N; Nawa H. 2003. Brain-derived neurotrophic factor signal enhances and maintains the expression of AMPA receptor-associated PDZ proteins in developing cortical neurons. Dev Biol 263(2):216-30. [PubMed: 14597197]  [MGI Ref ID J:86443]

Kohn J; Aloyz RS; Toma JG; Haak-Frendscho M; Miller FD. 1999. Functionally antagonistic interactions between the TrkA and p75 neurotrophin receptors regulate sympathetic neuron growth and target innervation. J Neurosci 19(13):5393-408. [PubMed: 10377349]  [MGI Ref ID J:120561]

Koizumi H; Hashimoto K; Iyo M. 2006. Dietary restriction changes behaviours in brain-derived neurotrophic factor heterozygous mice: role of serotonergic system. Eur J Neurosci 24(8):2335-44. [PubMed: 17074054]  [MGI Ref ID J:117120]

Kokaia M; Ernfors P; Kokaia Z; Elmer E; Jaenisch R; Lindvall O. 1995. Suppressed epileptogenesis in BDNF mutant mice. Exp Neurol 133(2):215-24. [PubMed: 7649227]  [MGI Ref ID J:28137]

Linnarsson S; Bjorklund A; Ernfors P. 1997. Learning deficit in BDNF mutant mice. Eur J Neurosci 9(12):2581-7. [PubMed: 9517463]  [MGI Ref ID J:46745]

Linnarsson S; Willson CA; Ernfors P. 2000. Cell death in regenerating populations of neurons in BDNF mutant mice. Brain Res Mol Brain Res 75(1):61-9. [PubMed: 10648888]  [MGI Ref ID J:59901]

Liu X; Ernfors P; Wu H; Jaenisch R. 1995. Sensory but not motor neuron deficits in mice lacking NT4 and BDNF. Nature 375(6528):238-41. [PubMed: 7746325]  [MGI Ref ID J:25566]

Liu X; Jaenisch R. 2000. Severe peripheral sensory neuron loss and modest motor neuron reduction in mice with combined deficiency of brain-derived neurotrophic factor, neurotrophin 3 and neurotrophin 4/5. Dev Dyn 218(1):94-101. [PubMed: 10822262]  [MGI Ref ID J:62072]

Lyckman AW; Fan G; Rios M; Jaenisch R; Sur M. 2005. Normal eye-specific patterning of retinal inputs to murine subcortical visual nuclei in the absence of brain-derived neurotrophic factor. Vis Neurosci 22(1):27-36. [PubMed: 15842738]  [MGI Ref ID J:105280]

McGough NN; He DY; Logrip ML; Jeanblanc J; Phamluong K; Luong K; Kharazia V; Janak PH; Ron D. 2004. RACK1 and brain-derived neurotrophic factor: a homeostatic pathway that regulates alcohol addiction. J Neurosci 24(46):10542-52. [PubMed: 15548669]  [MGI Ref ID J:96588]

Nagano T; Yanagawa Y; Obata K; Narisawa-Saito M; Namba H; Otsu Y; Takei N; Nawa H. 2003. Brain-derived neurotrophic factor upregulates and maintains AMPA receptor currents in neocortical GABAergic neurons. Mol Cell Neurosci 24(2):340-56. [PubMed: 14572457]  [MGI Ref ID J:126205]

Nosrat CA; Blomlof J; ElShamy WM; Ernfors P; Olson L. 1997. Lingual deficits in BDNF and NT3 mutant mice leading to gustatory and somatosensory disturbances, respectively. Development 124(7):1333-42. [PubMed: 9118804]  [MGI Ref ID J:40033]

Okayasu I; Yamada Y; Maeda T; Yoshida N; Koga Y; Oi K. 2004. The involvement of brain-derived neurotrophic factor in the pattern generator of mastication. Brain Res 1016(1):40-7. [PubMed: 15234250]  [MGI Ref ID J:91229]

Olofsdotter K; Lindvall O; Asztely F. 2000. Increased synaptic inhibition in dentate gyrus of mice with reduced levels of endogenous brain-derived neurotrophic factor. Neuroscience 101(3):531-9. [PubMed: 11113302]  [MGI Ref ID J:118727]

Pang PT; Teng HK; Zaitsev E; Woo NT; Sakata K; Zhen S; Teng KK; Yung WH; Hempstead BL; Lu B. 2004. Cleavage of proBDNF by tPA/plasmin is essential for long-term hippocampal plasticity. Science 306(5695):487-91. [PubMed: 15486301]  [MGI Ref ID J:105428]

Patterson SL; Abel T; Deuel TA; Martin KC; Rose JC; Kandel ER. 1996. Recombinant BDNF rescues deficits in basal synaptic transmission and hippocampal LTP in BDNF knockout mice. Neuron 16(6):1137-45. [PubMed: 8663990]  [MGI Ref ID J:110757]

Pineda JR; Canals JM; Bosch M; Adell A; Mengod G; Artigas F; Ernfors P; Alberch J. 2005. Brain-derived neurotrophic factor modulates dopaminergic deficits in a transgenic mouse model of Huntington's disease. J Neurochem 93(5):1057-68. [PubMed: 15934928]  [MGI Ref ID J:99427]

Pozzo-Miller LD; Gottschalk W; Zhang L; McDermott K; Du J; Gopalakrishnan R; Oho C; Sheng ZH; Lu B. 1999. Impairments in high-frequency transmission, synaptic vesicle docking, and synaptic protein distribution in the hippocampus of BDNF knockout mice. J Neurosci 19(12):4972-83. [PubMed: 10366630]  [MGI Ref ID J:55493]

Ren-Patterson RF; Cochran LW; Holmes A; Sherrill S; Huang SJ; Tolliver T; Lesch KP; Lu B; Murphy DL. 2005. Loss of brain-derived neurotrophic factor gene allele exacerbates brain monoamine deficiencies and increases stress abnormalities of serotonin transporter knockout mice. J Neurosci Res 79(6):756-71. [PubMed: 15672416]  [MGI Ref ID J:109152]

Rice FL; Albers KM; Davis BM; Silos-Santiago I; Wilkinson GA; LeMaster AM; Ernfors P; Smeyne RJ; Aldskogius H; Phillips HS; Barbacid M; DeChiara TM; Yancopoulos GD; Dunne CE; Fundin BT. 1998. Differential dependency of unmyelinated and A delta epidermal and upper dermal innervation on neurotrophins, trk receptors, and p75LNGFR. Dev Biol 198(1):57-81. [PubMed: 9640332]  [MGI Ref ID J:107715]

Ringstedt T; Linnarsson S; Wagner J; Lendahl U; Kokaia Z; Arenas E; Ernfors P; Ibanez CF. 1998. BDNF regulates reelin expression and Cajal-Retzius cell development in the cerebral cortex. Neuron 21(2):305-15. [PubMed: 9728912]  [MGI Ref ID J:49433]

Schuhmann B; Dietrich A; Sel S; Hahn C; Klingenspor M; Lommatzsch M; Gudermann T; Braun A; Renz H; Nockher WA. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol 163(1-2):15-23. [PubMed: 15885304]  [MGI Ref ID J:101880]

Schwartz PM; Borghesani PR; Levy RL; Pomeroy SL; Segal RA. 1997. Abnormal cerebellar development and foliation in BDNF-/- mice reveals a role for neurotrophins in CNS patterning. Neuron 19(2):269-81. [PubMed: 9292718]  [MGI Ref ID J:42627]

Sedy J; Szeder V; Walro JM; Ren ZG; Nanka O; Tessarollo L; Sieber-Blum M; Grim M; Kucera J. 2004. Pacinian corpuscle development involves multiple Trk signaling pathways. Dev Dyn 231(3):551-63. [PubMed: 15376326]  [MGI Ref ID J:93853]

Stenqvist A; Agerman K; Marmigere F; Minichiello L; Ernfors P. 2005. Genetic evidence for selective neurotrophin 3 signalling through TrkC but not TrkB in vivo. EMBO Rep 6(10):973-8. [PubMed: 16142215]  [MGI Ref ID J:101705]

Sun H; Oakley B. 2002. Development of anterior gustatory epithelia in the palate and tongue requires epidermal growth factor receptor. Dev Biol 242(1):31-43. [PubMed: 11795938]  [MGI Ref ID J:74312]

Torres-Peraza J; Pezzi S; Canals JM; Gavalda N; Garcia-Martinez JM; Perez-Navarro E; Alberch J. 2007. Mice heterozygous for neurotrophin-3 display enhanced vulnerability to excitotoxicity in the striatum through increased expression of N-methyl-D-aspartate receptors. Neuroscience 144(2):462-71. [PubMed: 17081696]  [MGI Ref ID J:117952]

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Ward NL; Hagg T. 2000. BDNF is needed for postnatal maturation of basal forebrain and neostriatum cholinergic neurons in vivo. Exp Neurol 162(2):297-310. [PubMed: 10739636]  [MGI Ref ID J:61704]

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Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	The Bdnftm1Jae strain is maintained by mating heterozygous mice to normal wildtype siblings. Heterozygous mice and normal wildtype siblings may be ordered. Expected coat colors from breeding:Black, White Bellied Agouti, Albino

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
		This strain is a mixture of 129, BALB/c, and C57BL/6 inbred strains. Wildtype mice from the colony may be used as controls.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.