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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+6p
Generation DefinitionsDonating Investigator Barbara Knowles, The Jackson Laboratory Description
Mice homozygous for the Cd4tm1Knw targeted mutation have a significant block in CD4+ T cell development; 90% of their circulating T cells are CD8+. Homozygous mutant mice also show a Class II restricted deficit in helper T cell activity and other T cell responses.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Cd4tm1Knw allele
002269 B6.129S6-Cd4tm1Knw/J 003090 NOD.129S6(B6)-Cd4tm1Knw/DvsJ View Strains carrying Cd4tm1Knw (2 strains)
002447 B10.129S2(B6)-Cd4tm1Litt/J 010514 B10.Cg-H2g Tg(Cd4-Klra1)6295Dl/J 002663 B6.129S2-Cd4tm1Mak/J 002269 B6.129S6-Cd4tm1Knw/J 002664 B6;129S-Cd4tm1Mak Cd8atm1Mak/J 012895 C57BL/6-Tg(Cd4-Il17rb)5Cdon/J 014639 C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J 014644 C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J 006483 CBy.129S2(B6)-Cd4tm1Mak/J 003090 NOD.129S6(B6)-Cd4tm1Knw/DvsJ 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Cd4tm1Knw/Cd4tm1Knw
B6.129S-Cd4tm1Knw/J
- immune system phenotype
- decreased CD4-positive T cell number
- significantly decreased CD4+ cell population in spleen and thymus as compared to control (MGI Ref ID J:87828)
- increased CD8-positive T cell number
- increased CD8+ cell population in spleen and thymus as compared to control (MGI Ref ID J:87828)
- increased susceptibility to parasitic infection
- significantly increased clearance time (24-50 days) after infection by Rickettsiales member (E. chaffeensis) (MGI Ref ID J:87828)
- granulomatous inflammation in liver is more extensive at 16 days post-infection in contrast to control (MGI Ref ID J:87828)
- delayed CTL response following second challenge with E. chaffeensis infection (MGI Ref ID J:87828)
- hematopoietic system phenotype
- decreased CD4-positive T cell number
- significantly decreased CD4+ cell population in spleen and thymus as compared to control (MGI Ref ID J:87828)
- increased CD8-positive T cell number
- increased CD8+ cell population in spleen and thymus as compared to control (MGI Ref ID J:87828)
This mouse can be used to support research in many areas including:Cd4tm1Knw related
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Cd4tm1Knw | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Barbara B Knowles | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | CD4-; CD4D; | ||
| Mutation Made By | Barbara Knowles, The Jackson Laboratory | ||
| Strain of Origin | 129S/SvEv-Gpi1 | ||
| ES Cell Line Name | CCE/EK.CCE | ||
| ES Cell Line Strain | 129S/SvEv-Gpi1 | ||
| Gene Symbol and Name | Cd4, CD4 antigen | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | CD4mut; L3T4; Ly-4; W3/25; lymphocyte antigen 4; p55; | ||
| Molecular Note | A neomycin resistance gene was inserted into exon 5. [MGI Ref ID J:48775] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cd4, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
McCarrick JW 3rd; Parnes JR; Seong RH; Solter D; Knowles BB. 1993. Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2(4):183-90. [PubMed: 8364601] [MGI Ref ID J:48775]
Additional References
Cd4tm1Knw relatedBommireddy R; Engle SJ; Ormsby I; Boivin GP; Babcock GF; Doetschman T. 2004. Elimination of both CD4(+) and CD8(+) T cells but not B cells eliminates inflammation and prolongs the survival of TGFbeta1-deficient mice. Cell Immunol 232(1-2):96-104. [PubMed: 15922720] [MGI Ref ID J:99033]
Borchers MT; Crosby J; Justice P; Farmer S; Hines E; Lee JJ; Lee NA. 2001. Intrinsic AHR in IL-5 transgenic mice is dependent on CD4(+) cells and CD49d-mediated signaling. Am J Physiol Lung Cell Mol Physiol 281(3):L653-9. [PubMed: 11504693] [MGI Ref ID J:132284]
Brochard V; Combadiere B; Prigent A; Laouar Y; Perrin A; Beray-Berthat V; Bonduelle O; Alvarez-Fischer D; Callebert J; Launay JM; Duyckaerts C; Flavell RA; Hirsch EC; Hunot S. 2009. Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease. J Clin Invest 119(1):182-92. [PubMed: 19104149] [MGI Ref ID J:144702]
Chan T; Pek EA; Huth K; Ashkar AA. 2011. CD4(+) T-cells are important in regulating macrophage polarization in C57BL/6 wild-type mice. Cell Immunol 266(2):180-6. [PubMed: 21040907] [MGI Ref ID J:167012]
Crosby JR; Shen HH; Borchers MT; Justice JP; Ansay T; Lee JJ; Lee NA. 2002. Ectopic expression of IL-5 identifies an additional CD4(+) T cell mechanism of airway eosinophil recruitment. Am J Physiol Lung Cell Mol Physiol 282(1):L99-108. [PubMed: 11741821] [MGI Ref ID J:107840]
Ganta RR; Cheng C; Wilkerson MJ; Chapes SK. 2004. Delayed clearance of Ehrlichia chaffeensis infection in CD4+ T-cell knockout mice. Infect Immun 72(1):159-67. [PubMed: 14688093] [MGI Ref ID J:87828]
Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754] [MGI Ref ID J:93553]
Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890] [MGI Ref ID J:125272]
Hancock WW; Tsai TL; Madaio MP; Gasser DL. 2003. Cutting Edge: Multiple autoimmune pathways in kd/kd mice. J Immunol 171(6):2778-81. [PubMed: 12960297] [MGI Ref ID J:85380]
Justice JP; Crosby J; Borchers MT; Tomkinson A; Lee JJ; Lee NA. 2002. CD4(+) T cell-dependent airway mucus production occurs in response to IL-5 expression in lung. Am J Physiol Lung Cell Mol Physiol 282(5):L1066-74. [PubMed: 11943672] [MGI Ref ID J:108354]
Kassiotis G; Bauer J; Akassoglou K; Lassmann H; Kollias G; Probert L. 1999. A tumor necrosis factor-induced model of human primary demyelinating diseases develops in immunodeficient mice. Eur J Immunol 29(3):912-7. [PubMed: 10092095] [MGI Ref ID J:53471]
Kobrynski LJ; Sousa AO; Nahmias AJ; Lee FK. 2005. Cutting edge: antibody production to pneumococcal polysaccharides requires CD1 molecules and CD8+ T cells. J Immunol 174(4):1787-90. [PubMed: 15699104] [MGI Ref ID J:96541]
Lau LL; Spain LM. 2000. Altered aging-related thymic involution in T cell receptor transgenic, MHC-deficient, and CD4-deficient mice. Mech Ageing Dev 114(2):101-21. [PubMed: 10799708] [MGI Ref ID J:111547]
Morgan DJ; Nugent CT; Raveney BJ; Sherman LA. 2004. In a transgenic model of spontaneous autoimmune diabetes, expression of a protective class II MHC molecule results in thymic deletion of diabetogenic CD8+ T cells. J Immunol 172(2):1000-8. [PubMed: 14707073] [MGI Ref ID J:100010]
Murphy TJ; Choileain NN; Zang Y; Mannick JA; Lederer JA. 2005. CD4+CD25+ regulatory T cells control innate immune reactivity after injury. J Immunol 174(5):2957-63. [PubMed: 15728508] [MGI Ref ID J:97723]
Petrofsky M; Bermudez LE. 2005. CD4+ T cells but Not CD8+ or gammadelta+ lymphocytes are required for host protection against Mycobacterium avium infection and dissemination through the intestinal route. Infect Immun 73(5):2621-7. [PubMed: 15845464] [MGI Ref ID J:97613]
Russell MS; Dudani R; Krishnan L; Sad S. 2009. IFN-gamma expressed by T cells regulates the persistence of antigen presentation by limiting the survival of dendritic cells. J Immunol 183(12):7710-8. [PubMed: 19923462] [MGI Ref ID J:157498]
VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356] [MGI Ref ID J:72616]
Venet F; Chung CS; Huang X; Lomas-Neira J; Chen Y; Ayala A. 2009. Lymphocytes in the development of lung inflammation: a role for regulatory CD4+ T cells in indirect pulmonary lung injury. J Immunol 183(5):3472-80. [PubMed: 19641139] [MGI Ref ID J:151875]
Vieyra M; Leisman S; Raedler H; Kwan WH; Yang M; Strainic MG; Medof ME; Heeger PS. 2011. Complement regulates CD4 T-cell help to CD8 T cells required for murine allograft rejection. Am J Pathol 179(2):766-74. [PubMed: 21704012] [MGI Ref ID J:174402]
Yang R; Murillo FM; Delannoy MJ; Blosser RL; Yutzy WH 4th; Uematsu S; Takeda K; Akira S; Viscidi RP; Roden RB. 2005. B lymphocyte activation by human papillomavirus-like particles directly induces Ig class switch recombination via TLR4-MyD88. J Immunol 174(12):7912-9. [PubMed: 15944297] [MGI Ref ID J:100870]
Yang R; Wheeler CM; Chen X; Uematsu S; Takeda K; Akira S; Pastrana DV; Viscidi RP; Roden RB. 2005. Papillomavirus capsid mutation to escape dendritic cell-dependent innate immunity in cervical cancer. J Virol 79(11):6741-50. [PubMed: 15890912] [MGI Ref ID J:98389]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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