Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S6 via EK.CCE ES cell line Generation N10F27 (08-JAN-08)   Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Cd4^tm1Knw targeted mutation have a significant block in CD4⁺ T-cell development; 90% of their circulating T-cells are CD8⁺. Homozygous mutant mice also show a Class II restricted deficit in helper T-cell activity and other T-cell responses.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Cd4^tm1Knw allele

002268	B6;129S6-Cd4tm1Knw/J
003090	NOD.129S6(B6)-Cd4tm1Knw/DvsJ

View Strains carrying   Cd4^tm1Knw     (2 strains)

Strains carrying other alleles of Cd4

002447	B10.129S2(B6)-Cd4tm1Litt/J
002663	B6.129S2-Cd4tm1Mak/J
002664	B6;129S-Cd4tm1Mak Cd8atm1Mak/J
006483	CBy.129S2(B6)-Cd4tm1Mak/J
005328	NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J

View Strains carrying other alleles of Cd4     (5 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cd4^tm1Knw/Cd4^tm1Knw

        B6.129S-Cd4^tm1Knw/J

• immune system phenotype
• decreased CD4-positive T cell number (MGI Ref ID J:87828)
  • significantly decreased CD4+ cell population in spleen and thymus as compared to control
• increased CD8-positive T cell number (MGI Ref ID J:87828)
  • increased CD8+ cell population in spleen and thymus as compared to control
• increased susceptibility to parasitic infection (MGI Ref ID J:87828)
  • significantly increased clearance time (24-50 days) after infection by Rickettsiales member (E. chaffeensis)
  • granulomatous inflammation in liver is more extensive at 16 days post-infection in contrast to control
  • delayed CTL response following second challenge with E. chaffeensis infection
• hematopoietic system phenotype
• decreased CD4-positive T cell number (MGI Ref ID J:87828)
  • significantly decreased CD4+ cell population in spleen and thymus as compared to control
• increased CD8-positive T cell number (MGI Ref ID J:87828)
  • increased CD8+ cell population in spleen and thymus as compared to control

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cd4^tm1Knw related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd4tm1Knw
Allele Name		targeted mutation 1, Barbara B Knowles
Allele Type		Targeted (knock-out)
Common Name(s)		CD4-; CD4D;
Mutation Made By		Barbara Knowles,   The Jackson Laboratory
Strain of Origin		129S/SvEv-Gpi1
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Cd4, CD4 antigen
Chromosome		6
Gene Common Name(s)		CD4mut; L3T4; Ly-4; W3/25; lymphocyte antigen 4; p55;
Molecular Note		A neomycin resistance gene was inserted into exon 5. [MGI Ref ID J:48775]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cd4, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

McCarrick JW 3d; Parnes JR; Seong RH; Solter D; Knowles BB. 1993. Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2(4):183-90. [PubMed: 8364601]  [MGI Ref ID J:48775]

Additional References

Mohammed KA; Nasreen N; Ward MJ; Antony VB. 2000. Induction of acute pleural inflammation by Staphylococcus aureus. I. CD4+ T cells play a critical role in experimental empyema. J Infect Dis 181(5):1693-9. [PubMed: 10823770]  [MGI Ref ID J:62683]

Schell TD; Knowles BB; Tevethia SS. 2000. Sequential loss of cytotoxic T lymphocyte responses to simian virus 40 large T antigen epitopes in T antigen transgenic mice developing osteosarcomas. Cancer Res 60(11):3002-12. [PubMed: 10850449]  [MGI Ref ID J:62541]

VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356]  [MGI Ref ID J:72616]

Cd4^tm1Knw related

Bommireddy R; Engle SJ; Ormsby I; Boivin GP; Babcock GF; Doetschman T. 2004. Elimination of both CD4(+) and CD8(+) T cells but not B cells eliminates inflammation and prolongs the survival of TGFbeta1-deficient mice. Cell Immunol 232(1-2):96-104. [PubMed: 15922720]  [MGI Ref ID J:99033]

Borchers MT; Crosby J; Justice P; Farmer S; Hines E; Lee JJ; Lee NA. 2001. Intrinsic AHR in IL-5 transgenic mice is dependent on CD4(+) cells and CD49d-mediated signaling. Am J Physiol Lung Cell Mol Physiol 281(3):L653-9. [PubMed: 11504693]  [MGI Ref ID J:132284]

Crosby JR; Shen HH; Borchers MT; Justice JP; Ansay T; Lee JJ; Lee NA. 2002. Ectopic expression of IL-5 identifies an additional CD4(+) T cell mechanism of airway eosinophil recruitment. Am J Physiol Lung Cell Mol Physiol 282(1):L99-108. [PubMed: 11741821]  [MGI Ref ID J:107840]

Ganta RR; Cheng C; Wilkerson MJ; Chapes SK. 2004. Delayed clearance of Ehrlichia chaffeensis infection in CD4+ T-cell knockout mice. Infect Immun 72(1):159-67. [PubMed: 14688093]  [MGI Ref ID J:87828]

Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754]  [MGI Ref ID J:93553]

Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890]  [MGI Ref ID J:125272]

Hancock WW; Tsai TL; Madaio MP; Gasser DL. 2003. Cutting Edge: Multiple autoimmune pathways in kd/kd mice. J Immunol 171(6):2778-81. [PubMed: 12960297]  [MGI Ref ID J:85380]

Justice JP; Crosby J; Borchers MT; Tomkinson A; Lee JJ; Lee NA. 2002. CD4(+) T cell-dependent airway mucus production occurs in response to IL-5 expression in lung. Am J Physiol Lung Cell Mol Physiol 282(5):L1066-74. [PubMed: 11943672]  [MGI Ref ID J:108354]

Kassiotis G; Bauer J; Akassoglou K; Lassmann H; Kollias G; Probert L. 1999. A tumor necrosis factor-induced model of human primary demyelinating diseases develops in immunodeficient mice. Eur J Immunol 29(3):912-7. [PubMed: 10092095]  [MGI Ref ID J:53471]

Kobrynski LJ; Sousa AO; Nahmias AJ; Lee FK. 2005. Cutting edge: antibody production to pneumococcal polysaccharides requires CD1 molecules and CD8+ T cells. J Immunol 174(4):1787-90. [PubMed: 15699104]  [MGI Ref ID J:96541]

Lau LL; Spain LM. 2000. Altered aging-related thymic involution in T cell receptor transgenic, MHC-deficient, and CD4-deficient mice. Mech Ageing Dev 114(2):101-21. [PubMed: 10799708]  [MGI Ref ID J:111547]

Morgan DJ; Nugent CT; Raveney BJ; Sherman LA. 2004. In a transgenic model of spontaneous autoimmune diabetes, expression of a protective class II MHC molecule results in thymic deletion of diabetogenic CD8+ T cells. J Immunol 172(2):1000-8. [PubMed: 14707073]  [MGI Ref ID J:100010]

Murphy TJ; Choileain NN; Zang Y; Mannick JA; Lederer JA. 2005. CD4+CD25+ regulatory T cells control innate immune reactivity after injury. J Immunol 174(5):2957-63. [PubMed: 15728508]  [MGI Ref ID J:97723]

Petrofsky M; Bermudez LE. 2005. CD4+ T cells but Not CD8+ or gammadelta+ lymphocytes are required for host protection against Mycobacterium avium infection and dissemination through the intestinal route. Infect Immun 73(5):2621-7. [PubMed: 15845464]  [MGI Ref ID J:97613]

VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356]  [MGI Ref ID J:72616]

Yang R; Murillo FM; Delannoy MJ; Blosser RL; Yutzy WH th; Uematsu S; Takeda K; Akira S; Viscidi RP; Roden RB. 2005. B lymphocyte activation by human papillomavirus-like particles directly induces Ig class switch recombination via TLR4-MyD88. J Immunol 174(12):7912-9. [PubMed: 15944297]  [MGI Ref ID J:100870]

Yang R; Wheeler CM; Chen X; Uematsu S; Takeda K; Akira S; Pastrana DV; Viscidi RP; Roden RB. 2005. Papillomavirus capsid mutation to escape dendritic cell-dependent innate immunity in cervical cancer. J Virol 79(11):6741-50. [PubMed: 15890912]  [MGI Ref ID J:98389]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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