Strain Name:

B6.129S-Fn1tm1Hyn/J

Stock Number:

002270

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S2/SvPas via D3 ES cell line
 
Donating InvestigatorDr. Richard Hynes,   Massachusetts Institute of Technology

Description
Mice homozygous for the Fn1tm1Hyn targeted mutation die during early embryonic development. Blastocyst development and implantation of homozygotes is normal. Gastrulation is initiated and appears normal, including extensive mesodermal movement. From embryonic day 8 onwards homozygous mutant embryos deteriorate through the 10th and 11th days of gestation. Homozygous mutant embryos have a shortened anterior-posterior axes, fail to develop a notochord or somites, and have abnormal development of the heart, blood vessels, and yolk sac indicating a general deficiency in mesodermally derived tissues. Heterozygous mice are viable for at least 2 years and appear healthy and approximately the same size as wild-type littermates. Plasma levels of fibronectin in heterozygotes are 50% lower than normal wildtype siblings.

Development
A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. This mutation was created in 129S2/SvPas-derived D3 embryonic stem (ES) cells. The mice were bred to 129S4/SvJae then backcrossed to C57BL/6 for at least 11 generations.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Fn1tm1Hyn allele
008444   129S4.129S2(B6)-Fn1tm1Hyn/J
008445   B6.129S-Fn1tm1Hyn/2J
008443   D2.129S2(Cg)-Fn1tm1Hyn/J
View Strains carrying   Fn1tm1Hyn     (3 strains)

Strains carrying other alleles of Fn1
008595   B6;129P2-Fn1tm4Hyn/J
View Strains carrying other alleles of Fn1     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Glomerulopathy with Fibronectin Deposits 2; GFND2   (FN1)
Plasma Fibronectin Deficiency   (FN1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Fn1tm1Hyn/Fn1tm1Hyn

        involves: 129S2/SvPas * C57BL/6J
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • homozygotes develop severe embryonic abnormalities from E8.0 onwards and undergo degeneration during E10 and E11   (MGI Ref ID J:16247)
  • cardiovascular system phenotype
  • abnormal dorsal aorta morphology
    • at E8.5, severely affected embryos show absence of dorsal aortae while less severely affected embryos exhibit distended dorsal aortae containing only a few blood cells   (MGI Ref ID J:16247)
  • abnormal endocardium morphology
    • at E8.5, the mutant endocardium is either indistinguishable from the thickened myocardium or absent   (MGI Ref ID J:16247)
  • abnormal heart development
    • at E8.5, 13 of 20 mutant embryos have a visible primitive heart whereas the remaining seven do not   (MGI Ref ID J:16247)
    • in severely affected embryos, heart primordia fail to fuse and are positioned laterally   (MGI Ref ID J:16247)
    • abnormal cardiac jelly morphology
      • at E8.5, cardiac jelly is deficient   (MGI Ref ID J:16247)
  • abnormal vasculogenesis
    • at E8.5, homozygotes display defective vasculogenesis in the yolk sac   (MGI Ref ID J:16247)
    • mutant embryonic vessels appear variable and deformed while extraembryonic vasculature is also defective   (MGI Ref ID J:16247)
  • absent vitelline blood vessels
    • at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac   (MGI Ref ID J:16247)
  • thick myocardium
    • at E8.5, homozygotes display a thickened myocardium   (MGI Ref ID J:16247)
  • embryogenesis phenotype
  • abnormal developmental patterning   (MGI Ref ID J:16247)
    • abnormal mesoderm development
      • at E8.0, homozygotes exhibit a deficit in the trunk and headfold mesoderm   (MGI Ref ID J:16247)
      • by E8.5, mutant headfolds appear small and misshapen, with a deficit in underlying mesoderm and many pyknotic cells   (MGI Ref ID J:16247)
      • abnormal lateral plate mesoderm morphology
        • by E8.5, lateral mesoderm flanking the neural tube is reduced   (MGI Ref ID J:16247)
    • failure of initiation of embryo turning
      • at E8.5, none of the mutant embryos have initiated turning   (MGI Ref ID J:16247)
    • short rostral-caudal axis
      • at E8.0, homozygotes display a shortened anterior-posterior axis   (MGI Ref ID J:16247)
  • abnormal embryonic tissue morphology   (MGI Ref ID J:16247)
    • abnormal mesoderm development
      • at E8.0, homozygotes exhibit a deficit in the trunk and headfold mesoderm   (MGI Ref ID J:16247)
      • by E8.5, mutant headfolds appear small and misshapen, with a deficit in underlying mesoderm and many pyknotic cells   (MGI Ref ID J:16247)
      • abnormal lateral plate mesoderm morphology
        • by E8.5, lateral mesoderm flanking the neural tube is reduced   (MGI Ref ID J:16247)
    • abnormal neural tube morphology/development   (MGI Ref ID J:16247)
      • abnormal embryonic neuroepithelium morphology
        • at E8.0, homozygotes display multiple bends and distortions in the neural ectoderm   (MGI Ref ID J:16247)
      • kinked neural tube
        • at E8.5, homozygotes display a kinked neural tube   (MGI Ref ID J:16247)
    • absent notochord
      • at E8.5, homozygotes lack an organized notochord; instead, the endodermal lining of the future midgut is juxtaposed to the neural tube   (MGI Ref ID J:16247)
    • absent somites
      • at E8.5, mutant embryos lack somites whereas wild-type embryos contain 8 to 12 pairs of somites; however, condensations of cells suggestive of incipient somites are detected in 3 of 20 mutants   (MGI Ref ID J:16247)
  • abnormal extraembryonic tissue morphology   (MGI Ref ID J:16247)
    • abnormal amnion morphology
      • at E7.5, mutant embryos possess three germ layers and normal extraembryonic membranes except for a concave amnion   (MGI Ref ID J:16247)
      • at E8.0, the mutant amnion is undersized and closely apposed to the embryo, displaying a pressure deficit in the amniotic cavity   (MGI Ref ID J:16247)
    • abnormal visceral yolk sac morphology
      • at E8.5, the mesodermal and endodermal layers of the mutant yolk sac appear to split apart   (MGI Ref ID J:16247)
      • absent visceral yolk sac blood islands   (MGI Ref ID J:16247)
    • absent vitelline blood vessels
      • at E8.5, blood vessels are detected in the exocoelomic cavity rather than in the yolk sac   (MGI Ref ID J:16247)
    • failure of chorioallantoic fusion
      • at E8.5, the allantois has yet not fused with the chorion   (MGI Ref ID J:16247)
  • decreased embryo size
    • at E7.5, mutant embryos appear relatively normal, though slightly smaller than wild-type embryos   (MGI Ref ID J:16247)
  • embryonic growth retardation
    • starting at E8.0, homozygotes appear developmentally retarded and abnormal   (MGI Ref ID J:16247)
  • nervous system phenotype
  • abnormal neural tube morphology/development   (MGI Ref ID J:16247)
    • abnormal embryonic neuroepithelium morphology
      • at E8.0, homozygotes display multiple bends and distortions in the neural ectoderm   (MGI Ref ID J:16247)
    • kinked neural tube
      • at E8.5, homozygotes display a kinked neural tube   (MGI Ref ID J:16247)
  • growth/size/body phenotype
  • decreased embryo size
    • at E7.5, mutant embryos appear relatively normal, though slightly smaller than wild-type embryos   (MGI Ref ID J:16247)
  • embryonic growth retardation
    • starting at E8.0, homozygotes appear developmentally retarded and abnormal   (MGI Ref ID J:16247)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Fn1tm1Hyn related

Cardiovascular Research
Heart Abnormalities
Vascular Defects

Developmental Biology Research
Defects in Extracellular Matrix Molecules
Internal/Organ Defects
      heart
      heart: vasculature

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Fn1tm1Hyn
Allele Name targeted mutation 1, Richard Hynes
Allele Type Targeted (Null/Knockout)
Common Name(s) FN.null; FNnull; Fn-;
Mutation Made ByDr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Fn1, fibronectin 1
Chromosome 1
Gene Common Name(s) CIG; ED-B; FIBNEC; FINC; FN; FNZ; Fn-1; GFND; GFND2; LETS; MSF;
Molecular Note A PGK-neomycin resistance cassette replaced 0.8 kb of the gene including the translation initiation site and part of the signaling sequence. Plasma concentrations of fibronectin in heterozygotes were one-half those of wild-type littermates. The encoded protein was not detectable in immunoprecipitations from cultures of homozygous mutant E7.5 embryos. [MGI Ref ID J:16247]

Genotyping

Genotyping Information

Genotyping Protocols

Fn1tm1Hyn, Standard PCR
Fn1tm1Hyn, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

George EL; Georges-Labouesse EN; Patel-King RS; Rayburn H; Hynes RO. 1993. Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin. Development 119(4):1079-91. [PubMed: 8306876]  [MGI Ref ID J:16247]

Additional References

George EL; Baldwin HS; Hynes RO. 1997. Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. Blood 90(8):3073-81. [PubMed: 9376588]  [MGI Ref ID J:43434]

Georges-Labouesse EN; George EL; Rayburn H; Hynes RO. 1996. Mesodermal development in mouse embryos mutant for fibronectin. Dev Dyn 207(2):145-56. [PubMed: 8906418]  [MGI Ref ID J:36210]

Fn1tm1Hyn related

Astrof S; Kirby A; Lindblad-Toh K; Daly M; Hynes RO. 2007. Heart development in fibronectin-null mice is governed by a genetic modifier on chromosome four. Mech Dev 124(7-8):551-8. [PubMed: 17628448]  [MGI Ref ID J:134424]

George EL; Baldwin HS; Hynes RO. 1997. Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. Blood 90(8):3073-81. [PubMed: 9376588]  [MGI Ref ID J:43434]

Georges-Labouesse EN; George EL; Rayburn H; Hynes RO. 1996. Mesodermal development in mouse embryos mutant for fibronectin. Dev Dyn 207(2):145-56. [PubMed: 8906418]  [MGI Ref ID J:36210]

Ilic D; Kovacic B; Johkura K; Schlaepfer DD; Tomasevic N; Han Q; Kim JB; Howerton K; Baumbusch C; Ogiwara N; Streblow DN; Nelson JA; Dazin P; Shino Y; Sasaki K; Damsky CH. 2004. FAK promotes organization of fibronectin matrix and fibrillar adhesions. J Cell Sci 117(Pt 2):177-87. [PubMed: 14657279]  [MGI Ref ID J:87891]

Mittal A; Pulina M; Hou SY; Astrof S. 2010. Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest. Mech Dev 127(9-12):472-84. [PubMed: 20807571]  [MGI Ref ID J:165711]

Mittal A; Pulina M; Hou SY; Astrof S. 2013. Fibronectin and integrin alpha 5 play requisite roles in cardiac morphogenesis. Dev Biol 381(1):73-82. [PubMed: 23791818]  [MGI Ref ID J:200776]

Pulina MV; Hou SY; Mittal A; Julich D; Whittaker CA; Holley SA; Hynes RO; Astrof S. 2011. Essential roles of fibronectin in the development of the left-right embryonic body plan. Dev Biol 354(2):208-20. [PubMed: 21466802]  [MGI Ref ID J:173656]

Taverna D; Ullman-Cullere M; Rayburn H; Bronson RT; Hynes RO. 1998. A test of the role of alpha5 integrin/fibronectin interactions in tumorigenesis. Cancer Res 58(4):848-53. [PubMed: 9485045]  [MGI Ref ID J:45769]

Yang JT; Bader BL; Kreidberg JA; Ullman-Cullere M; Trevithick JE; Hynes RO. 1999. Overlapping and independent functions of fibronectin receptor integrins in early mesodermal development. Dev Biol 215(2):264-77. [PubMed: 10545236]  [MGI Ref ID J:58411]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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