Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Stanley Korsmeyer,   Dana-Farber Cancer Institute

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Tlx1^tm1Sjk allele

002771

B6.129S2-Tlx1tm1Sjk/J

View Strains carrying   Tlx1^tm1Sjk     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tlx1^tm1Sjk/Tlx1^tm1Sjk

        involves: 129S2/SvPas

• hematopoietic system phenotype
• absent spleen (MGI Ref ID J:17695)
  • absence of spleen in adult mice
  • embryos showed no cellular organiziation at the site of splenic development
• immune system phenotype
• absent spleen (MGI Ref ID J:17695)
  • absence of spleen in adult mice
  • embryos showed no cellular organiziation at the site of splenic development

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tlx1^tm1Sjk related

Developmental Biology Research
Internal/Organ Defects (asplenic)

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Internal/Organ Research
Spleen Defects (asplenic)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tlx1tm1Sjk
Allele Name		targeted mutation 1, Stanley J Korsmeyer
Allele Type		Targeted (knock-out)
Common Name(s)		Hox11-; Tlx-; Tlx1-;
Mutation Made By		Stanley Korsmeyer,   Dana-Farber Cancer Institute
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Tlx1, T-cell leukemia, homeobox 1
Chromosome		19
Gene Common Name(s)		HOX11; Hox-11; MGC163402; RGD1563655; TCL3; homeo box-11 cluster;
Molecular Note		Exon 1 was disrupted by the insertion of a neomycin selection cassette into codon 53. Transcript was undetected by in situ hybridization of homozygous mutant mice. [MGI Ref ID J:17695]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Roberts CW; Shutter JR; Korsmeyer SJ. 1994. Hox11 controls the genesis of the spleen. Nature 368(6473):747-9. [PubMed: 7908720]  [MGI Ref ID J:17695]

Additional References

Tlx1^tm1Sjk related

Brendolan A; Ferretti E; Salsi V; Moses K; Quaggin S; Blasi F; Cleary ML; Selleri L. 2005. A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny. Development 132(13):3113-26. [PubMed: 15944191]  [MGI Ref ID J:99062]

Cheng L; Arata A; Mizuguchi R; Qian Y; Karunaratne A; Gray PA; Arata S; Shirasawa S; Bouchard M; Luo P; Chen CL; Busslinger M; Goulding M; Onimaru H; Ma Q. 2004. Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates. Nat Neurosci 7(5):510-7. [PubMed: 15064766]  [MGI Ref ID J:90585]

Herzer U; Crocoll A; Barton D; Howells N; Englert C. 1999. The Wilms tumor suppressor gene wt1 is required for development of the spleen. Curr Biol 9(15):837-40. [PubMed: 10469569]  [MGI Ref ID J:56651]

Karrer U; Althage A; Odermatt B; Roberts CW; Korsmeyer SJ; Miyawaki S ; Hengartner H ; Zinkernagel RM. 1997. On the key role of secondary lymphoid organs in antiviral immune responses studied in alymphoplastic (aly/aly) and spleenless (Hox11(-)/-) mutant mice. J Exp Med 185(12):2157-70. [PubMed: 9182687]  [MGI Ref ID J:41084]

Lakkis FG; Arakelov A; Konieczny BT; Inoue Y. 2000. Immunologic 'ignorance' of vascularized organ transplants in the absence of secondary lymphoid tissue. Nat Med 6(6):686-8. [PubMed: 10835686]  [MGI Ref ID J:118716]

Moens CB; Selleri L. 2006. Hox cofactors in vertebrate development. Dev Biol 291(2):193-206. [PubMed: 16515781]  [MGI Ref ID J:109563]

Qian Y; Shirasawa S; Chen CL; Cheng L; Ma Q. 2002. Proper development of relay somatic sensory neurons and D2/D4 interneurons requires homeobox genes Rnx/Tlx-3 and Tlx-1. Genes Dev 16(10):1220-33. [PubMed: 12023301]  [MGI Ref ID J:76655]

Roberts CW; Sonder AM; Lumsden A; Korsmeyer SJ. 1995. Development expression of Hox11 and specification of splenic cell fate. Am J Pathol 146(5):1089-101. [PubMed: 7747804]  [MGI Ref ID J:25198]

Wardemann H; Boehm T; Dear N; Carsetti R. 2002. B-1a B cells that link the innate and adaptive immune responses are lacking in the absence of the spleen. J Exp Med 195(6):771-80. [PubMed: 11901202]  [MGI Ref ID J:75706]

Xu Y; Lopes C; Qian Y; Liu Y; Cheng L; Goulding M; Turner EE; Lima D; Ma Q. 2008. Tlx1 and Tlx3 coordinate specification of dorsal horn pain-modulatory peptidergic neurons. J Neurosci 28(15):4037-46. [PubMed: 18400903]  [MGI Ref ID J:134327]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.