Strain Name:

B6;129S2-Tlx1tm1Sjk/J

Stock Number:

002273

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Stanley J. Korsmeyer,   Dana-Farber Cancer Institute

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tlx1tm1Sjk allele
002771   B6.129S2-Tlx1tm1Sjk/J
019142   NOD.129S2(B6)-Tlx1tm1Sjk/DvsJ
View Strains carrying   Tlx1tm1Sjk     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tlx1tm1Sjk/Tlx1tm1Sjk

        involves: 129S2/SvPas
  • hematopoietic system phenotype
  • abnormal spleen development
    • at E12.5, mutant embryos display no cellular organization or mesenchymal cell condensation at the site of splenic development   (MGI Ref ID J:17695)
    • at E13.5, mutant embryos show no histological evidence of a splenic primordium within the dorsal mesogastrium   (MGI Ref ID J:17695)
    • abnormal spleen mesenchyme morphology
      • at E12.5, mutant embryos display no mesenchymal cell condensation at the site of splenic development, indicating a specific, localized defect in mesodermal cells destined to form the spleen   (MGI Ref ID J:17695)
  • absent spleen
    • all adult homozygotes appear externally normal but display complete absence of spleen   (MGI Ref ID J:17695)
    • in contrast, all other internal organs (including the stomach and pancreas) are normal in morphology and position   (MGI Ref ID J:17695)
  • increased leukocyte cell number
    • homozygotes display a ~2-fold increase in the number of white blood cells relative to wild-type mice   (MGI Ref ID J:17695)
    • however, the B-cell and T-cell profile is normal in thymus, lymph node and peripheral blood   (MGI Ref ID J:17695)
    • increased lymphocyte cell number
      • in peripheral blood smears   (MGI Ref ID J:17695)
    • increased neutrophil cell number
      • in peripheral blood smears   (MGI Ref ID J:17695)
  • increased number of Howell-Jolly bodies
    • many erythrocytes contain nuclear fragments of condensed DNA known as Howell-Jolly bodies, commonly associated with asplenia   (MGI Ref ID J:17695)
    • however, complete blood counts indicate that the number of red blood cells is normal   (MGI Ref ID J:17695)
  • immune system phenotype
  • abnormal spleen development
    • at E12.5, mutant embryos display no cellular organization or mesenchymal cell condensation at the site of splenic development   (MGI Ref ID J:17695)
    • at E13.5, mutant embryos show no histological evidence of a splenic primordium within the dorsal mesogastrium   (MGI Ref ID J:17695)
    • abnormal spleen mesenchyme morphology
      • at E12.5, mutant embryos display no mesenchymal cell condensation at the site of splenic development, indicating a specific, localized defect in mesodermal cells destined to form the spleen   (MGI Ref ID J:17695)
  • absent spleen
    • all adult homozygotes appear externally normal but display complete absence of spleen   (MGI Ref ID J:17695)
    • in contrast, all other internal organs (including the stomach and pancreas) are normal in morphology and position   (MGI Ref ID J:17695)
  • increased leukocyte cell number
    • homozygotes display a ~2-fold increase in the number of white blood cells relative to wild-type mice   (MGI Ref ID J:17695)
    • however, the B-cell and T-cell profile is normal in thymus, lymph node and peripheral blood   (MGI Ref ID J:17695)
    • increased lymphocyte cell number
      • in peripheral blood smears   (MGI Ref ID J:17695)
    • increased neutrophil cell number
      • in peripheral blood smears   (MGI Ref ID J:17695)
  • embryogenesis phenotype
  • *normal* embryogenesis phenotype
    • despite observed gene expression in the branchial region of wild-type mice, homozygotes display no detectable abnormalities in branchial arches   (MGI Ref ID J:17695)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • despite observed gene expression in the developing hindbrain and cranial ganglia of wild-type mice, homozygotes display no detectable abnormalities in the hindbrain, cranial ganglia or cranial motor nuclei   (MGI Ref ID J:17695)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tlx1tm1Sjk related

Developmental Biology Research
Internal/Organ Defects
      asplenic

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Internal/Organ Research
Spleen Defects
      asplenic

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tlx1tm1Sjk
Allele Name targeted mutation 1, Stanley J Korsmeyer
Allele Type Targeted (knock-out)
Common Name(s) Hox11-; Tlx-; Tlx1-;
Mutation Made ByDr. Stanley Korsmeyer,   Dana-Farber Cancer Institute
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Tlx1, T cell leukemia, homeobox 1
Chromosome 19
Gene Common Name(s) HOX11; Hox-11; RGD1563655; TCL3; homeo box-11 cluster;
Molecular Note Exon 1 was disrupted by the insertion of a neomycin selection cassette into codon 53. Transcript was undetected by in situ hybridization of homozygous mutant mice. [MGI Ref ID J:17695]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Roberts CW; Shutter JR; Korsmeyer SJ. 1994. Hox11 controls the genesis of the spleen. Nature 368(6473):747-9. [PubMed: 7908720]  [MGI Ref ID J:17695]

Additional References

Tlx1tm1Sjk related

Brendolan A; Ferretti E; Salsi V; Moses K; Quaggin S; Blasi F; Cleary ML; Selleri L. 2005. A Pbx1-dependent genetic and transcriptional network regulates spleen ontogeny. Development 132(13):3113-26. [PubMed: 15944191]  [MGI Ref ID J:99062]

Cheng L; Arata A; Mizuguchi R; Qian Y; Karunaratne A; Gray PA; Arata S; Shirasawa S; Bouchard M; Luo P; Chen CL; Busslinger M; Goulding M; Onimaru H; Ma Q. 2004. Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates. Nat Neurosci 7(5):510-7. [PubMed: 15064766]  [MGI Ref ID J:90585]

Guo Z; Zhao C; Huang M; Huang T; Fan M; Xie Z; Chen Y; Zhao X; Xia G; Geng J; Cheng L. 2012. Tlx1/3 and ptf1a control the expression of distinct sets of transmitter and Peptide receptor genes in the developing dorsal spinal cord. J Neurosci 32(25):8509-20. [PubMed: 22723691]  [MGI Ref ID J:185665]

Herzer U; Crocoll A; Barton D; Howells N; Englert C. 1999. The Wilms tumor suppressor gene wt1 is required for development of the spleen. Curr Biol 9(15):837-40. [PubMed: 10469569]  [MGI Ref ID J:56651]

Hu J; Huang T; Li T; Guo Z; Cheng L. 2012. c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections. J Neurosci 32(16):5362-73. [PubMed: 22514301]  [MGI Ref ID J:184447]

Huang M; Huang T; Xiang Y; Xie Z; Chen Y; Yan R; Xu J; Cheng L. 2008. Ptf1a, Lbx1 and Pax2 coordinate glycinergic and peptidergic transmitter phenotypes in dorsal spinal inhibitory neurons. Dev Biol 322(2):394-405. [PubMed: 18634777]  [MGI Ref ID J:142008]

Karrer U; Althage A; Odermatt B; Roberts CW; Korsmeyer SJ; Miyawaki S ; Hengartner H ; Zinkernagel RM. 1997. On the key role of secondary lymphoid organs in antiviral immune responses studied in alymphoplastic (aly/aly) and spleenless (Hox11(-)/-) mutant mice. J Exp Med 185(12):2157-70. [PubMed: 9182687]  [MGI Ref ID J:41084]

Lakkis FG; Arakelov A; Konieczny BT; Inoue Y. 2000. Immunologic 'ignorance' of vascularized organ transplants in the absence of secondary lymphoid tissue. Nat Med 6(6):686-8. [PubMed: 10835686]  [MGI Ref ID J:118716]

Moens CB; Selleri L. 2006. Hox cofactors in vertebrate development. Dev Biol 291(2):193-206. [PubMed: 16515781]  [MGI Ref ID J:109563]

Qian Y; Shirasawa S; Chen CL; Cheng L; Ma Q. 2002. Proper development of relay somatic sensory neurons and D2/D4 interneurons requires homeobox genes Rnx/Tlx-3 and Tlx-1. Genes Dev 16(10):1220-33. [PubMed: 12023301]  [MGI Ref ID J:76655]

Roberts CW; Sonder AM; Lumsden A; Korsmeyer SJ. 1995. Development expression of Hox11 and specification of splenic cell fate. Am J Pathol 146(5):1089-101. [PubMed: 7747804]  [MGI Ref ID J:25198]

Wardemann H; Boehm T; Dear N; Carsetti R. 2002. B-1a B cells that link the innate and adaptive immune responses are lacking in the absence of the spleen. J Exp Med 195(6):771-80. [PubMed: 11901202]  [MGI Ref ID J:75706]

Xu Y; Lopes C; Qian Y; Liu Y; Cheng L; Goulding M; Turner EE; Lima D; Ma Q. 2008. Tlx1 and Tlx3 coordinate specification of dorsal horn pain-modulatory peptidergic neurons. J Neurosci 28(15):4037-46. [PubMed: 18400903]  [MGI Ref ID J:134327]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.5)