Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S2 via D3 ES cell line Generation N11 (01-JAN-06)   Donating Investigator Richard Hynes,   Massachusetts Institute of Technology

Description
Mice homozygous for the Itga5^tm1Hyn targeted mutation die during embryonic development. Homozygous mutant embryos exhibit defects in the vasculature of the yolk sac and the embyro as well as severe defects in posterior and extraembryonic mesoderm. Implantation and initiation of gastrulation and neurulation is normal. There is normal development of notochord, somite and considerable development of brain, optic and otic anlagen and branchial arches.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Itga5^tm1Hyn/Itga5^tm1Hyn

        involves: 129S2/SvPas * C57BL/6J

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:16248)
  • homozygotes die between E10.0 and E11.0
• embryogenesis phenotype
• abnormal embryonic tissue morphology (MGI Ref ID J:16248)
• failure of somite differentiation (MGI Ref ID J:16248)
  • at E9.5, mutant embryos lack somites in the truncated posterior regions
  • in contrast, normal somite numbers (7-10 pairs) are noted anteriorly
  • at E8.0-E8.5, most mutant embryos exhibit normal numbers of somites relative to wild-type embryos (4-13 pairs)
• abnormal extraembryonic tissue morphology (MGI Ref ID J:16248)
• abnormal vitelline vasculature (MGI Ref ID J:16248)
  • at E9.5, mutant yolk sacs display abnormal blood vessels, with large numbers of blood cells leaking into the exocoelomic space
• abnormal mesoderm development (MGI Ref ID J:16248)
  • at E9.5, the extraembryonic mesodermal layer is reduced and separated from the extraembryonic endoderm
• abnormal paraxial mesoderm (MGI Ref ID J:16248)
  • at E9.5, homozygotes display a deficit in paraxial mesoderm in the region flanking the neural tube
  • mesenchymeal cells, normally found on the dorsal side of somites are absent
• abnormal rostral-caudal axis patterning (MGI Ref ID J:16248)
  • at E8.5, homozygotes display an abnormal curvature and a slightly truncated anterior-posterior axis
  • at E9.5, the posterior trunk of mutant embryos is deformed and only 1/3 of E9.0 wild-type length
  • although delayed, the anterior region (including heads, branchial arches and heart) appears relatively unaffected
• embryonic growth retardation (MGI Ref ID J:16248)
  • at E9.5, mutant embryos are developmentally retarded and equivalent in size to E9.0 wild-type embryos
  • by E10.5, mutant embryos are equivalent in size to E9.5 wild-type embryos
• incomplete embryo turning (MGI Ref ID J:16248)
  • at E9.5, mutant embryos are only partially turned into the fetal position
• growth/size phenotype
• embryonic growth retardation (MGI Ref ID J:16248)
  • at E9.5, mutant embryos are developmentally retarded and equivalent in size to E9.0 wild-type embryos
  • by E10.5, mutant embryos are equivalent in size to E9.5 wild-type embryos
• nervous system phenotype
• kinked neural tube (MGI Ref ID J:16248)
  • at E9.5, homozygotes exhibit kinked neural tubes in the posterior trunk region
• cardiovascular system phenotype
• abnormal vasculature (MGI Ref ID J:16248)
  • all homozygotes form hearts and a vascular system; however, primitive blood vessels are distended and leaky
  • at E9.5, homozygotes show vascular defects both in the embryo and in extraembryonic vasculature, with fewer blood cells present in the heart and blood vessels
• abnormal dorsal aorta morphology (MGI Ref ID J:16248)
  • at E9.5, the mutant dorsal aortae are not fully closed, with leakage of primitive blood cells into the mesoderm-deficient space
• cellular phenotype
• *normal* cellular phenotype (MGI Ref ID J:16248)
  • mutant embryonic fibroblasts are able to assemble fibronectin matrix, form focal contacts, and migrate on fibronectin

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Itga5^tm1Hyn related

Cancer Research
Genes Regulating Growth and Proliferation

Cardiovascular Research
Heart Abnormalities
Vascular Defects

Cell Biology Research
Cell Cycle Regulation
Cell Motility Defects
Defects in Cell Adhesion Molecules
Defects in Extracellular Matrix Molecules

Developmental Biology Research
Cell Motility Defects
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
Mesodermal Defects
Neural Crest Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Internal/Organ Research
Heart Abnormalities

Research Tools
Apoptosis Research
Cell Biology Research
Developmental Biology Research
Hematological Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Itga5tm1Hyn
Allele Name		targeted mutation 1, Richard Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		alpha5-; alpha5-null;
Mutation Made By		Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Itga5, integrin alpha 5 (fibronectin receptor alpha)
Chromosome		15
Gene Common Name(s)		CD49e; FNRA; Fnra; Itga5_retired; VLA5A; fibronectin receptor alpha (Itga5);
Molecular Note		A neomycin resistance cassette replaced 330 bp of the gene, deleting most of the first exon. [MGI Ref ID J:16248]

Genotyping

Genotyping Information

Genotyping Protocols

Itga5^tm1Hyn, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Yang JT; Rayburn H; Hynes RO. 1993. Embryonic mesodermal defects in alpha 5 integrin-deficient mice. Development 119(4):1093-105. [PubMed: 7508365]  [MGI Ref ID J:16248]

Additional References

Goh KL; Yang JT; Hynes RO. 1997. Mesodermal defects and cranial neural crest apoptosis in alpha5 integrin-null embryos. Development 124(21):4309-19. [PubMed: 9334279]  [MGI Ref ID J:44237]

Itga5^tm1Hyn related

Chan CS; Weeber EJ; Kurup S; Sweatt JD; Davis RL. 2003. Integrin requirement for hippocampal synaptic plasticity and spatial memory. J Neurosci 23(18):7107-16. [PubMed: 12904471]  [MGI Ref ID J:84858]

Goh KL; Yang JT; Hynes RO. 1997. Mesodermal defects and cranial neural crest apoptosis in alpha5 integrin-null embryos. Development 124(21):4309-19. [PubMed: 9334279]  [MGI Ref ID J:44237]

Haack H; Hynes RO. 2001. Integrin receptors are required for cell survival and proliferation during development of the peripheral glial lineage. Dev Biol 233(1):38-55. [PubMed: 11319856]  [MGI Ref ID J:69286]

Taverna D; Ullman-Cullere M; Rayburn H; Bronson RT; Hynes RO. 1998. A test of the role of alpha5 integrin/fibronectin interactions in tumorigenesis. Cancer Res 58(4):848-53. [PubMed: 9485045]  [MGI Ref ID J:45769]

Yang JT; Bader BL; Kreidberg JA; Ullman-Cullere M; Trevithick JE; Hynes RO. 1999. Overlapping and independent functions of fibronectin receptor integrins in early mesodermal development. Dev Biol 215(2):264-77. [PubMed: 10545236]  [MGI Ref ID J:58411]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.