Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S7 via AB2.1 ES cell line Generation N10F44 (27-NOV-11) Generation Definitions   Donating Investigator Arthur Beaudet,   Baylor College of Medicine

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Selp^tm1Bay mutation are viable and fertile. Homoyzgous mutant mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (2-3-fold). Circulating lymphocyte and monocyte counts are normal. 60-70% reduction in neutrophil emigration into the peritoneum during Streptococcus pneumoniae-induced peritonitis is reduced 60-70%. It can be used as model for human leukocyte adhesion deficiency I syndrome (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Selp^tm1Bay allele

002285

B6.129S7-Icam1tm1Bay Selptm1Bay/J

View Strains carrying   Selp^tm1Bay     (1 strain)

Strains carrying other alleles of Selp

008439	B6.129S2-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008437	B6.129S2-Seletm1Hyn Selptm1Hyn/J
008441	B6.129S2-Selltm3Hyn Selptm1Hyn/J
008432	B6.129S2-Selptm1Hyn/J
002285	B6.129S7-Icam1tm1Bay Selptm1Bay/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
003805	B6;129S-Selltm3Hyn Selptm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
002217	B6;129S2-Selptm1Hyn/J
008440	C.129S2(B6)-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J
008442	C.129S2(B6)-Selltm3Hyn Selptm1Hyn/J
008433	C.129S2(B6)-Selptm1Hyn/J

View Strains carrying other alleles of Selp     (13 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Selp^tm1Bay/Selp^tm1Bay

        B6.129S7-Selp^tm1Bay/J

• immune system phenotype
• abnormal T cell physiology
  • T lymphocyte migration and accumulation into cutaneous inflammatory regions induced by a combination of IFN-gamma and TNF-alpha is inhibited by about 25%, however IFN-gamma, TNF-alpha, and ConA, has no effect on T cell infiltration   (MGI Ref ID J:113536)

Selp^tm1Bay/Selp^tm1Bay

        either: B6.129S7-Selp^tm1Bay or (involves: 129S7/SvEvBrd * C57BL/6)

• hematopoietic system phenotype
• increased leukocyte cell number
  • systemic leukocyte counts are ~9800/ul (~61% neutrophils, ~37% lymphocytes) compared to wild-type counts of ~5660/ul (53% neutrophils, 46% lymphocytes)   (MGI Ref ID J:31374)
• immune system phenotype
• abnormal leukocyte tethering or rolling
  • after surgery to exteriorize cremaster muscle, mice do not display leukocyte rolling for the first hour, but a significant amount of rolling is detected after 2 hours (~8% rolling leukocyte flux)   (MGI Ref ID J:31374)
  • leukocytes show intermittent interactions with endothelium causing an increase in rolling velocity to ~129 um/second (~35 um/second in wild-type)   (MGI Ref ID J:31374)
  • treatment with Tnfa (tumor necrosis factor alpha) reduces rolling velocity to ~5 um/second   (MGI Ref ID J:31374)
• increased leukocyte cell number
  • systemic leukocyte counts are ~9800/ul (~61% neutrophils, ~37% lymphocytes) compared to wild-type counts of ~5660/ul (53% neutrophils, 46% lymphocytes)   (MGI Ref ID J:31374)
• cellular phenotype
• abnormal leukocyte tethering or rolling
  • after surgery to exteriorize cremaster muscle, mice do not display leukocyte rolling for the first hour, but a significant amount of rolling is detected after 2 hours (~8% rolling leukocyte flux)   (MGI Ref ID J:31374)
  • leukocytes show intermittent interactions with endothelium causing an increase in rolling velocity to ~129 um/second (~35 um/second in wild-type)   (MGI Ref ID J:31374)
  • treatment with Tnfa (tumor necrosis factor alpha) reduces rolling velocity to ~5 um/second   (MGI Ref ID J:31374)

Selp^tm1Bay/Selp^tm1Bay

        B6.129S7-Selp^tm1Bay

• immune system phenotype
• decreased mast cell number
  • during the chronic phase of antigen exposure   (MGI Ref ID J:121083)
• decreased susceptibility to type IV hypersensitivity reaction
  • chronic inflammation in response to oxazolone exposure is inhibited compared to in similarly treated wild-type mice   (MGI Ref ID J:121083)
  • however, acute inflammation in response to oxazolone exposure is normal   (MGI Ref ID J:121083)
• hematopoietic system phenotype
• decreased mast cell number
  • during the chronic phase of antigen exposure   (MGI Ref ID J:121083)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Selp^tm1Bay/Selp^tm1Bay

        involves: 129S7/SvEvBrd * C57BL/6J

• immune system phenotype
• abnormal leukocyte migration
  • Streptococcus pneumoniae induced peritonitis reduced neutrophil emigration into peritoneum by 61-69% after 2-4 hours   (MGI Ref ID J:24239)
• abnormal spleen morphology
  • increase in splenic thrombosis and necrosis in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
• eye inflammation
  • panophthalmitis observed in 8% of mice following infection with S. pneumoniae   (MGI Ref ID J:101979)
• increased leukocyte cell number
  • elevated peripheral leukocyte count in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
• • increased neutrophil cell number
    • mild neutrophilia in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
    • 2-3 fold increase in numbers of circulating neutrophils   (MGI Ref ID J:24239)
• increased susceptibility to bacterial infection
  • severity and duration of morbidity higher than wild-type after infection with Streptococcus pneumoniae   (MGI Ref ID J:101979)
  • survival rate was 19% in contrast to a 57% rate for wild-type mice   (MGI Ref ID J:101979)
  • decreased ability to clear bacteremia among survivors   (MGI Ref ID J:101979)
• behavior/neurological phenotype
• abnormal pilomotor reflex
  • observed following infection with S. pneumoniae   (MGI Ref ID J:101979)
• head tilt
  • observed following infection with S. pneumoniae   (MGI Ref ID J:101979)
• hypoactivity
  • observed following infection with S. pneumoniae   (MGI Ref ID J:101979)
• spinning
  • observed following infection with S. pneumoniae   (MGI Ref ID J:101979)
• tremors
  • observed following infection with S. pneumoniae   (MGI Ref ID J:101979)
• liver/biliary system phenotype
• hepatic necrosis
  • higher incidence in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
• growth/size phenotype
• weight loss
  • observed 48 hours post-infection with S. pneumoniae   (MGI Ref ID J:101979)
• vision/eye phenotype
• eye inflammation
  • panophthalmitis observed in 8% of mice following infection with S. pneumoniae   (MGI Ref ID J:101979)
• hematopoietic system phenotype
• abnormal spleen morphology
  • increase in splenic thrombosis and necrosis in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
• increased leukocyte cell number
  • elevated peripheral leukocyte count in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
• • increased neutrophil cell number
    • mild neutrophilia in contrast to controls following infection with S. pneumoniae   (MGI Ref ID J:101979)
    • 2-3 fold increase in numbers of circulating neutrophils   (MGI Ref ID J:24239)
• cellular phenotype
• abnormal leukocyte migration
  • Streptococcus pneumoniae induced peritonitis reduced neutrophil emigration into peritoneum by 61-69% after 2-4 hours   (MGI Ref ID J:24239)

Selp^tm1Bay/Selp^tm1Bay

        D1.129S7(B6J)-Selp^tm1Bay

• skeleton phenotype
• increased susceptibility to induced arthritis
  • accelerated incidence of induced arthritis, 23% compared to 2% of DBA/1 mice by 28 days   (MGI Ref ID J:57086)
  • increase in number of affected paws   (MGI Ref ID J:57086)
  • consistently higher arthritis severity scores as determined by inflammation, paw/digit distortion and ankylosis   (MGI Ref ID J:57086)
  • onset of arthritis occurs as early as day 14 with a median onset of 34 days   (MGI Ref ID J:57086)
• immune system phenotype
• abnormal cytokine secretion
  • increased levels of IL10 and IL5 measured in stimulated splenocytes   (MGI Ref ID J:57086)
  • decreased levels of IL2 and IL4 measured in stimulated splenocytes   (MGI Ref ID J:57086)
• increased IgG level
  • increased levels of serum IgG anti-type II collagen antibody measured in collagen immunized mice as compared to immunized DBA/1 mice   (MGI Ref ID J:57086)
• increased susceptibility to induced arthritis
  • accelerated incidence of induced arthritis, 23% compared to 2% of DBA/1 mice by 28 days   (MGI Ref ID J:57086)
  • increase in number of affected paws   (MGI Ref ID J:57086)
  • consistently higher arthritis severity scores as determined by inflammation, paw/digit distortion and ankylosis   (MGI Ref ID J:57086)
  • onset of arthritis occurs as early as day 14 with a median onset of 34 days   (MGI Ref ID J:57086)

Selp^tm1Bay/Selp^tm1Bay

        involves: 129S7/SvEvBrd * C57BL/6

• hematopoietic system phenotype
• increased neutrophil cell number
  • circulating neutrophils are increased in mutants and this increases with age of mice   (MGI Ref ID J:112286)
• immune system phenotype
• increased neutrophil cell number
  • circulating neutrophils are increased in mutants and this increases with age of mice   (MGI Ref ID J:112286)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Selp^tm1Bay related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Selptm1Bay
Allele Name		targeted mutation 1, Baylor College of Medicine
Allele Type		Targeted (knock-out)
Common Name(s)		CD62ELP-; CD62P-; P-; P-sel-; P-selectin-;
Mutation Made By		Arthur Beaudet,   Baylor College of Medicine
Strain of Origin		129S7/SvEvBrd-Hprt
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt
Gene Symbol and Name		Selp, selectin, platelet
Chromosome		1
Gene Common Name(s)		CD62; CD62P; GMP140; GRMP; Grmp; LECAM3; P-selectin; PADGEM; PSEL; PSELECT; granulocyte membrane protein;
Molecular Note		A neomycin resistance gene was inserted in the reverse orientation to delete a 4.5kb region containing exons 3 through 5. RT-PCR analysis of lung RNA isolated from homozygous mutant mice showed a transcript 682 base pairs shorter than normal. Immunohistochemical staining showed a lack of encoded protein on both endothelium and platelets. [MGI Ref ID J:24239]

Genotyping

Genotyping Information

Genotyping Protocols

Selp^tm1Bay, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bullard DC; Qin L; Lorenzo I; Quinlin WM; Doyle NA; Bosse R; Vestweber D; Doerschuk CM; Beaudet AL. 1995. P-selectin/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli [see comments] J Clin Invest 95(4):1782-8. [PubMed: 7535798]  [MGI Ref ID J:24239]

Additional References

Bullard DC; Kunkel EJ; Kubo H; Hicks MJ; Lorenzo I; Doyle NA; Doerschuk CM; Ley K; Beaudet AL. 1996. Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice. J Exp Med 183(5):2329-36. [PubMed: 8642341]  [MGI Ref ID J:33135]

Bullard DC; Mobley JM; Justen JM; Sly LM; Chosay JG; Dunn CJ; Lindsey JR; Beaudet AL; Staite ND. 1999. Acceleration and increased severity of collagen-induced arthritis in P-selectin mutant mice. J Immunol 163(5):2844-9. [PubMed: 10453030]  [MGI Ref ID J:57086]

Doerschuk CM; Quinlan WM; Doyle NA; Bullard DC; Vestweber D; Jones ML; Takei F; Ward PA; Beaudet AL. 1996. The role of P-selectin and ICAM-1 in acute lung injury as determined using blocking antibodies and mutant mice. J Immunol 157(10):4609-14. [PubMed: 8906840]  [MGI Ref ID J:37197]

Huo Y; Schober A; Forlow SB; Smith DF; Hyman MC; Jung S; Littman DR; Weber C; Ley K. 2003. Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E. Nat Med 9(1):61-7. [PubMed: 12483207]  [MGI Ref ID J:81164]

Jung U; Ley K. 1999. Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin. J Immunol 162(11):6755-62. [PubMed: 10352295]  [MGI Ref ID J:55032]

Kamochi M; Kamochi F; Kim YB; Sawh S; Sanders JM; Sarembock I; Green S; Young JS; Ley K; Fu SM; Rose CE Jr. 1999. P-selectin and ICAM-1 mediate endotoxin-induced neutrophil recruitment and injury to the lung and liver. Am J Physiol 277(2 Pt 1):L310-9. [PubMed: 10444525]  [MGI Ref ID J:56844]

Kohm AP; Miller SD. 2003. Role of ICAM-1 and P-selectin expression in the development and effector function of CD4+CD25+regulatory T cells. J Autoimmun 21(3):261-71. [PubMed: 14599851]  [MGI Ref ID J:86435]

Sun G; Chang WL; Li J; Berney SM; Kimpel D; van der Heyde HC. 2003. Inhibition of platelet adherence to brain microvasculature protects against severe Plasmodium berghei malaria. Infect Immun 71(11):6553-61. [PubMed: 14573677]  [MGI Ref ID J:86276]

Tesfaigzi Y; Fischer MJ; Daheshia M; Green FH; De Sanctis GT; Wilder JA. 2002. Bax is crucial for IFN-gamma-induced resolution of allergen-induced mucus cell metaplasia. J Immunol 169(10):5919-25. [PubMed: 12421976]  [MGI Ref ID J:80074]

Selp^tm1Bay related

Baker PJ; DuFour L; Dixon M; Roopenian DC. 2000. Adhesion molecule deficiencies increase Porphyromonas gingivalis-induced alveolar bone loss in mice. Infect Immun 68(6):3103-7. [PubMed: 10816450]  [MGI Ref ID J:62266]

Bartholdy C; Marker O; Thomsen AR. 2000. Migration of activated CD8(+) T lymphocytes to sites of viral infection does not require endothelial selectins. Blood 95(4):1362-9. [PubMed: 10666212]  [MGI Ref ID J:110273]

Bonasio R; Scimone ML; Schaerli P; Grabie N; Lichtman AH; von Andrian UH. 2006. Clonal deletion of thymocytes by circulating dendritic cells homing to the thymus. Nat Immunol 7(10):1092-100. [PubMed: 16951687]  [MGI Ref ID J:112629]

Bonig H; Priestley GV; Oehler V; Papayannopoulou T. 2007. Hematopoietic progenitor cells (HPC) from mobilized peripheral blood display enhanced migration and marrow homing compared to steady-state bone marrow HPC. Exp Hematol 35(2):326-34. [PubMed: 17258081]  [MGI Ref ID J:123189]

Briaud SA; Ding ZM; Michael LH; Entman ML; Daniel S; Ballantyne CM. 2001. Leukocyte trafficking and myocardial reperfusion injury in ICAM-1/P-selectin-knockout mice. Am J Physiol Heart Circ Physiol 280(1):H60-7. [PubMed: 11123218]  [MGI Ref ID J:68056]

Broide DH; Humber D; Sullivan S; Sriramarao P. 1998. Inhibition of eosinophil rolling and recruitment in P-selectin- and intracellular adhesion molecule-1-deficient mice [published erratum appears in Blood 1998 Jul 1;92(1):343] Blood 91(8):2847-56. [PubMed: 9531595]  [MGI Ref ID J:47461]

Broide DH; Sullivan S; Gifford T; Sriramarao P. 1998. Inhibition of pulmonary eosinophilia in P-selectin- and ICAM-1-deficient mice. Am J Respir Cell Mol Biol 18(2):218-25. [PubMed: 9476909]  [MGI Ref ID J:114264]

Bullard DC; Kunkel EJ; Kubo H; Hicks MJ; Lorenzo I; Doyle NA; Doerschuk CM; Ley K; Beaudet AL. 1996. Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice. J Exp Med 183(5):2329-36. [PubMed: 8642341]  [MGI Ref ID J:33135]

Bullard DC; Mobley JM; Justen JM; Sly LM; Chosay JG; Dunn CJ; Lindsey JR; Beaudet AL; Staite ND. 1999. Acceleration and increased severity of collagen-induced arthritis in P-selectin mutant mice. J Immunol 163(5):2844-9. [PubMed: 10453030]  [MGI Ref ID J:57086]

Carlow DA; Ziltener HJ. 2006. CD43 deficiency has no impact in competitive in vivo assays of neutrophil or activated T cell recruitment efficiency. J Immunol 177(9):6450-9. [PubMed: 17056577]  [MGI Ref ID J:140506]

Carrithers MD; Visintin I; Viret C; Janeway CS Jr. 2002. Role of genetic background in P selectin-dependent immune surveillance of the central nervous system. J Neuroimmunol 129(1-2):51-7. [PubMed: 12161020]  [MGI Ref ID J:102957]

Collins RG; Jung U; Ramirez M; Bullard DC; Hicks MJ; Smith CW; Ley K; Beaudet AL. 2001. Dermal and pulmonary inflammatory disease in E-selectin and P-selectin double-null mice is reduced in triple-selectin-null mice. Blood 98(3):727-35. [PubMed: 11468173]  [MGI Ref ID J:70682]

Collins RG; Velji R; Guevara NV; Hicks MJ; Chan L; Beaudet AL. 2000. P-Selectin or intercellular adhesion molecule (ICAM)-1 deficiency substantially protects against atherosclerosis in apolipoprotein E-deficient mice. J Exp Med 191(1):189-94. [PubMed: 10620617]  [MGI Ref ID J:59249]

Curtis JL; Sonstein J; Craig RA; Todt JC; Knibbs RN; Polak T; Bullard DC; Stoolman LM. 2002. Subset-specific reductions in lung lymphocyte accumulation following intratracheal antigen challenge in endothelial selectin-deficient mice. J Immunol 169(5):2570-9. [PubMed: 12193727]  [MGI Ref ID J:120687]

Deban L; Russo RC; Sironi M; Moalli F; Scanziani M; Zambelli V; Cuccovillo I; Bastone A; Gobbi M; Valentino S; Doni A; Garlanda C; Danese S; Salvatori G; Sassano M; Evangelista V; Rossi B; Zenaro E; Constantin G; Laudanna C; Bottazzi B; Mantovani A. 2010. Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat Immunol 11(4):328-34. [PubMed: 20208538]  [MGI Ref ID J:158981]

Doerschuk CM; Quinlan WM; Doyle NA; Bullard DC; Vestweber D; Jones ML; Takei F; Ward PA; Beaudet AL. 1996. The role of P-selectin and ICAM-1 in acute lung injury as determined using blocking antibodies and mutant mice. J Immunol 157(10):4609-14. [PubMed: 8906840]  [MGI Ref ID J:37197]

Eppihimer MJ; Russell J; Anderson DC; Wolitzky BA; Granger DN. 1997. Endothelial cell adhesion molecule expression in gene-targeted mice. Am J Physiol 273(4 Pt 2):H1903-8. [PubMed: 9362259]  [MGI Ref ID J:43907]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Evangelista V; Manarini S; Coller BS; Smyth SS. 2003. Role of P-selectin, beta2-integrins, and Src tyrosine kinases in mouse neutrophil-platelet adhesion. J Thromb Haemost 1(5):1048-54. [PubMed: 12871376]  [MGI Ref ID J:104023]

Evangelista V; Pamuklar Z; Piccoli A; Manarini S; Dell'elba G; Pecce R; Martelli N; Federico L; Rojas M; Berton G; Lowell CA; Totani L; Smyth SS. 2007. Src family kinases mediate neutrophil adhesion to adherent platelets. Blood 109(6):2461-9. [PubMed: 17095622]  [MGI Ref ID J:145358]

Falati S; Liu Q; Gross P; Merrill-Skoloff G; Chou J; Vandendries E; Celi A; Croce K; Furie BC; Furie B. 2003. Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin. J Exp Med 197(11):1585-98. [PubMed: 12782720]  [MGI Ref ID J:83734]

Forlow SB; Foley PL; Ley K. 2002. Severely reduced neutrophil adhesion and impaired host defense against fecal and commensal bacteria in CD18-/-P-selectin-/- double null mice. FASEB J 16(12):1488-96. [PubMed: 12374771]  [MGI Ref ID J:120011]

Forlow SB; Ley K. 2001. Selectin-independent leukocyte rolling and adhesion in mice deficient in E-, P-, and L-selectin and ICAM-1. Am J Physiol Heart Circ Physiol 280(2):H634-41. [PubMed: 11158961]  [MGI Ref ID J:68044]

Forlow SB; White EJ; Thomas KL; Bagby GJ; Foley PL; Ley K. 2002. T cell requirement for development of chronic ulcerative dermatitis in e- and p-selectin-deficient mice. J Immunol 169(9):4797-804. [PubMed: 12391189]  [MGI Ref ID J:79774]

Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58. [PubMed: 17456770]  [MGI Ref ID J:121083]

Gossens K; Naus S; Corbel SY; Lin S; Rossi FM; Kast J; Ziltener HJ. 2009. Thymic progenitor homing and lymphocyte homeostasis are linked via S1P-controlled expression of thymic P-selectin/CCL25. J Exp Med 206(4):761-78. [PubMed: 19289576]  [MGI Ref ID J:147871]

Graciano AL; Bryant DD; White DJ; Horton J; Bowles NE; Giroir BP. 2001. Targeted disruption of ICAM-1, P-selectin genes improves cardiac function and survival in TNF-alpha transgenic mice. Am J Physiol Heart Circ Physiol 280(4):H1464-71. [PubMed: 11247755]  [MGI Ref ID J:108278]

Gregory JL; Morand EF; McKeown SJ; Ralph JA; Hall P; Yang YH; McColl SR; Hickey MJ. 2006. Macrophage migration inhibitory factor induces macrophage recruitment via CC chemokine ligand 2. J Immunol 177(11):8072-9. [PubMed: 17114481]  [MGI Ref ID J:140686]

Hafezi-Moghadam A; Thomas KL; Prorock AJ; Huo Y; Ley K. 2001. L-selectin shedding regulates leukocyte recruitment. J Exp Med 193(7):863-72. [PubMed: 11283159]  [MGI Ref ID J:120579]

He X; Schoeb TR; Panoskaltsis-Mortari A; Zinn KR; Kesterson RA; Zhang J; Samuel S; Hicks MJ; Hickey MJ; Bullard DC. 2006. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. J Immunol 177(12):8748-56. [PubMed: 17142777]  [MGI Ref ID J:127199]

Hickey MJ; Bullard DC; Issekutz A; James WG. 2002. Leukocyte-endothelial cell interactions are enhanced in dermal postcapillary venules of MRL/fas(lpr) (lupus-prone) mice: roles of P- and E-selectin. J Immunol 168(9):4728-36. [PubMed: 11971023]  [MGI Ref ID J:126009]

Hickey MJ; Granger DN; Kubes P. 1999. Molecular mechanisms underlying IL-4-induced leukocyte recruitment in vivo: a critical role for the alpha 4 integrin. J Immunol 163(6):3441-8. [PubMed: 10477616]  [MGI Ref ID J:119200]

Hicks AE; Abbitt KB; Dodd P; Ridger VC; Hellewell PG; Norman KE. 2005. The anti-inflammatory effects of a selectin ligand mimetic, TBC-1269, are not a result of competitive inhibition of leukocyte rolling in vivo. J Leukoc Biol 77(1):59-66. [PubMed: 15466915]  [MGI Ref ID J:95144]

Horikawa M; Fujimoto M; Hasegawa M; Matsushita T; Hamaguchi Y; Kawasuji A; Matsushita Y; Fujita T; Ogawa F; Takehara K; Steeber DA; Sato S. 2006. E- and P-selectins synergistically inhibit bleomycin-induced pulmonary fibrosis. Am J Pathol 169(3):740-9. [PubMed: 16936251]  [MGI Ref ID J:112363]

Huo Y; Schober A; Forlow SB; Smith DF; Hyman MC; Jung S; Littman DR; Weber C; Ley K. 2003. Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E. Nat Med 9(1):61-7. [PubMed: 12483207]  [MGI Ref ID J:81164]

Ishii T; Fujita T; Matsushita T; Yanaba K; Hasegawa M; Nakashima H; Ogawa F; Shimizu K; Takehara K; Tedder TF; Sato S; Fujimoto M. 2009. Establishment of experimental eosinophilic vasculitis by IgE-mediated cutaneous reverse passive arthus reaction. Am J Pathol 174(6):2225-33. [PubMed: 19389931]  [MGI Ref ID J:148778]

James WG; Bullard DC; Hickey MJ. 2003. Critical role of the alpha 4 integrin/VCAM-1 pathway in cerebral leukocyte trafficking in lupus-prone MRL/fas(lpr) mice. J Immunol 170(1):520-7. [PubMed: 12496439]  [MGI Ref ID J:127282]

Jones SP; Trocha SD; Strange MB; Granger DN; Kevil CG; Bullard DC; Lefer DJ. 2000. Leukocyte and endothelial cell adhesion molecules in a chronic murine model of myocardial reperfusion injury Am J Physiol Heart Circ Physiol 279(5):H2196-201. [PubMed: 11045953]  [MGI Ref ID J:65658]

Jung U; Ley K. 1999. Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin. J Immunol 162(11):6755-62. [PubMed: 10352295]  [MGI Ref ID J:55032]

Jung U; Ramos CL; Bullard DC; Ley K. 1998. Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion. Am J Physiol 274(5 Pt 2):H1785-91. [PubMed: 9612391]  [MGI Ref ID J:47723]

Kamochi M; Kamochi F; Kim YB; Sawh S; Sanders JM; Sarembock I; Green S; Young JS; Ley K; Fu SM; Rose CE Jr. 1999. P-selectin and ICAM-1 mediate endotoxin-induced neutrophil recruitment and injury to the lung and liver. Am J Physiol 277(2 Pt 1):L310-9. [PubMed: 10444525]  [MGI Ref ID J:56844]

Kerfoot SM; Long EM; Hickey MJ; Andonegui G; Lapointe BM; Zanardo RC; Bonder C; James WG; Robbins SM; Kubes P. 2004. TLR4 contributes to disease-inducing mechanisms resulting in central nervous system autoimmune disease. J Immunol 173(11):7070-7. [PubMed: 15557205]  [MGI Ref ID J:94363]

Kerfoot SM; Norman MU; Lapointe BM; Bonder CS; Zbytnuik L; Kubes P. 2006. Reevaluation of P-selectin and alpha 4 integrin as targets for the treatment of experimental autoimmune encephalomyelitis. J Immunol 176(10):6225-34. [PubMed: 16670333]  [MGI Ref ID J:131757]

Kim MH; Carter PR; Harris NR. 2007. P-selectin-mediated adhesion impairs endothelium-dependent arteriolar dilation in hypercholesterolemic mice. Am J Physiol Heart Circ Physiol 292(1):H632-8. [PubMed: 16980348]  [MGI Ref ID J:119942]

Kohm AP; Miller SD. 2003. Role of ICAM-1 and P-selectin expression in the development and effector function of CD4+CD25+regulatory T cells. J Autoimmun 21(3):261-71. [PubMed: 14599851]  [MGI Ref ID J:86435]

Komura K; Hasegawa M; Hamaguchi Y; Saito E; Kaburagi Y; Yanaba K; Kawara S; Takehara K; Seki M; Steeber DA; Tedder TF; Sato S. 2003. Ultraviolet light exposure suppresses contact hypersensitivity by abrogating endothelial intercellular adhesion molecule-1 up-regulation at the elicitation site. J Immunol 171(6):2855-62. [PubMed: 12960307]  [MGI Ref ID J:85377]

Kulidjian AA; Issekutz AC; Issekutz TB. 2002. Differential role of E-selectin and P-selectin in T lymphocyte migration to cutaneous inflammatory reactions induced by cytokines. Int Immunol 14(7):751-60. [PubMed: 12096034]  [MGI Ref ID J:113536]

Kuligowski MP; Kitching AR; Hickey MJ. 2006. Leukocyte recruitment to the inflamed glomerulus: a critical role for platelet-derived P-selectin in the absence of rolling. J Immunol 176(11):6991-9. [PubMed: 16709860]  [MGI Ref ID J:131787]

Kunkel EJ; Chomas JE; Ley K. 1998. Role of primary and secondary capture for leukocyte accumulation in vivo. Circ Res 82(1):30-8. [PubMed: 9440702]  [MGI Ref ID J:114235]

Kunkel EJ; Jung U; Bullard DC; Norman KE; Wolitzky BA; Vestweber D; Beaudet AL; Ley K. 1996. Absence of trauma-induced leukocyte rolling in mice deficient in both P-selectin and intercellular adhesion molecule 1. J Exp Med 183(1):57-65. [PubMed: 8551244]  [MGI Ref ID J:31374]

Kupatt C; Wichels R; Horstkotte J; Krombach F; Habazettl H; Boekstegers P. 2002. Molecular mechanisms of platelet-mediated leukocyte recruitment during myocardial reperfusion. J Leukoc Biol 72(3):455-61. [PubMed: 12223512]  [MGI Ref ID J:124451]

Lam FW; Burns AR; Smith CW; Rumbaut RE. 2011. Platelets enhance neutrophil transendothelial migration via P-selectin glycoprotein ligand-1. Am J Physiol Heart Circ Physiol 300(2):H468-75. [PubMed: 21169400]  [MGI Ref ID J:169607]

Larbi KY; Dangerfield JP; Culley FJ; Marshall D; Haskard DO; Jose PJ; Williams TJ; Nourshargh S. 2003. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses. J Leukoc Biol 73(1):65-73. [PubMed: 12525563]  [MGI Ref ID J:120684]

Laubli H; Stevenson JL; Varki A; Varki NM; Borsig L. 2006. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res 66(3):1536-42. [PubMed: 16452210]  [MGI Ref ID J:106671]

Lawson JA; Burns AR; Farhood A; Lynn Bajt M; Collins RG; Smith CW; Jaeschke H. 2000. Pathophysiologic importance of E- and L-selectin for neutrophil-induced liver injury during endotoxemia in mice. Hepatology 32(5):990-8. [PubMed: 11050049]  [MGI Ref ID J:106550]

Li Z; Burns AR; Han L; Rumbaut RE; Smith CW. 2011. IL-17 and VEGF Are Necessary for Efficient Corneal Nerve Regeneration. Am J Pathol 178(3):1106-16. [PubMed: 21356362]  [MGI Ref ID J:169522]

Li Z; Burns AR; Rumbaut RE; Smith CW. 2007. {gamma}{delta} T Cells Are Necessary for Platelet and Neutrophil Accumulation in Limbal Vessels and Efficient Epithelial Repair after Corneal Abrasion. Am J Pathol 171(3):838-845. [PubMed: 17675580]  [MGI Ref ID J:124350]

Li Z; Burns AR; Smith CW. 2006. Two waves of neutrophil emigration in response to corneal epithelial abrasion: distinct adhesion molecule requirements. Invest Ophthalmol Vis Sci 47(5):1947-55. [PubMed: 16639002]  [MGI Ref ID J:108504]

Li Z; Rumbaut RE; Burns AR; Smith CW. 2006. Platelet response to corneal abrasion is necessary for acute inflammation and efficient re-epithelialization. Invest Ophthalmol Vis Sci 47(11):4794-802. [PubMed: 17065490]  [MGI Ref ID J:123092]

Liu Z; Miner JJ; Yago T; Yao L; Lupu F; Xia L; McEver RP. 2010. Differential regulation of human and murine P-selectin expression and function in vivo. J Exp Med 207(13):2975-87. [PubMed: 21149548]  [MGI Ref ID J:176863]

Ludwig RJ; Bergmann P; Garbaraviciene J; von Stebut E; Radeke HH; Gille J; Diehl S; Hardt K; Henschler R; Kaufmann R; Pfeilschifter JM; Boehncke WH. 2010. Platelet, not endothelial, p-selectin expression contributes to generation of immunity in cutaneous contact hypersensitivity. Am J Pathol 176(3):1339-45. [PubMed: 20056837]  [MGI Ref ID J:158387]

Lukacs NW; John A; Berlin A; Bullard DC; Knibbs R; Stoolman LM. 2002. E- and P-selectins are essential for the development of cockroach allergen-induced airway responses. J Immunol 169(4):2120-5. [PubMed: 12165540]  [MGI Ref ID J:120695]

Manka D; Collins RG; Ley K; Beaudet AL; Sarembock IJ. 2001. Absence of p-selectin, but not intercellular adhesion molecule-1, attenuates neointimal growth after arterial injury in apolipoprotein e-deficient mice. Circulation 103(7):1000-5. [PubMed: 11181476]  [MGI Ref ID J:103301]

Maugeri N; Malato S; Femia EA; Pugliano M; Campana L; Lunghi F; Rovere-Querini P; Lussana F; Podda G; Cattaneo M; Ciceri F; Manfredi AA. 2011. Clearance of circulating activated platelets in polycythemia vera and essential thrombocythemia. Blood 118(12):3359-66. [PubMed: 21705500]  [MGI Ref ID J:176907]

Mizgerd JP; Bullard DC; Hicks MJ; Beaudet AL; Doerschuk CM. 1999. Chronic inflammatory disease alters adhesion molecule requirements for acute neutrophil emigration in mouse skin. J Immunol 162(9):5444-8. [PubMed: 10228023]  [MGI Ref ID J:112286]

Mori M; Salter JW; Vowinkel T; Krieglstein CF; Stokes KY; Granger DN. 2005. Molecular determinants of the prothrombogenic phenotype assumed by inflamed colonic venules. Am J Physiol Gastrointest Liver Physiol 288(5):G920-6. [PubMed: 15550557]  [MGI Ref ID J:98225]

Munoz FM; Hawkins EP; Bullard DC; Beaudet AL; Kaplan SL. 1997. Host defense against systemic infection with Streptococcus pneumoniae is impaired in E-, P-, and E-/P-selectin-deficient mice. J Clin Invest 100(8):2099-106. [PubMed: 9329976]  [MGI Ref ID J:101979]

Myers D Jr; Farris D; Hawley A; Wrobleski S; Chapman A; Stoolman L; Knibbs R; Strieter R; Wakefield T. 2002. Selectins influence thrombosis in a mouse model of experimental deep venous thrombosis. J Surg Res 108(2):212-21. [PubMed: 12505044]  [MGI Ref ID J:103110]

Norman KE; Katopodis AG; Thoma G; Kolbinger F; Hicks AE; Cotter MJ; Pockley AG; Hellewell PG. 2000. P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo Blood 96(10):3585-91. [PubMed: 11071658]  [MGI Ref ID J:65810]

Osterholzer JJ; Ames T; Polak T; Sonstein J; Moore BB; Chensue SW; Toews GB; Curtis JL. 2005. CCR2 and CCR6, but not endothelial selectins, mediate the accumulation of immature dendritic cells within the lungs of mice in response to particulate antigen. J Immunol 175(2):874-83. [PubMed: 16002685]  [MGI Ref ID J:100694]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Patel KN; Soubra SH; Bellera RV; Dong JF; McMullen CA; Burns AR; Rumbaut RE. 2008. Differential role of von Willebrand factor and P-selectin on microvascular thrombosis in endotoxemia. Arterioscler Thromb Vasc Biol 28(12):2225-30. [PubMed: 18802014]  [MGI Ref ID J:159795]

Pelletier SD; Hong DS; Hu Y; Liu Y; Li S. 2004. Lack of the adhesion molecules P-selectin and intercellular adhesion molecule-1 accelerate the development of BCR/ABL-induced chronic myeloid leukemia-like myeloproliferative disease in mice. Blood 104(7):2163-71. [PubMed: 15213099]  [MGI Ref ID J:93772]

Perkowski S; Scherpereel A; Murciano JC; Arguiri E; Solomides CC; Albelda SM; Muzykantov V; Christofidou-Solomidou M. 2006. Dissociation between alveolar transmigration of neutrophils and lung injury in hyperoxia. Am J Physiol Lung Cell Mol Physiol 291(5):L1050-8. [PubMed: 16815892]  [MGI Ref ID J:144691]

Petri B; Broermann A; Li H; Khandoga AG; Zarbock A; Krombach F; Goerge T; Schneider SW; Jones C; Nieswandt B; Wild MK; Vestweber D. 2010. von Willebrand factor promotes leukocyte extravasation. Blood 116(22):4712-9. [PubMed: 20716766]  [MGI Ref ID J:166636]

Pierangeli SS; Espinola RG; Liu X; Harris EN. 2001. Thrombogenic effects of antiphospholipid antibodies are mediated by intercellular cell adhesion molecule-1, vascular cell adhesion molecule-1, and P-selectin. Circ Res 88(2):245-50. [PubMed: 11157679]  [MGI Ref ID J:115602]

Puri KD; Doggett TA; Huang CY; Douangpanya J; Hayflick JS; Turner M; Penninger J; Diacovo TG. 2005. The role of endothelial PI3Kgamma activity in neutrophil trafficking. Blood 106(1):150-7. [PubMed: 15769890]  [MGI Ref ID J:107472]

Raisanen-Sokolowski A; Glysing-Jensen T; Russell ME. 1999. Donor and recipient contributions of ICAM-1 and P-selectin in parenchymal rejection and graft arteriosclerosis: insights from double knockout mice. J Heart Lung Transplant 18(8):735-43. [PubMed: 10512519]  [MGI Ref ID J:59743]

Ramos CL; Huo Y; Jung U; Ghosh S; Manka DR; Sarembock IJ; Ley K. 1999. Direct demonstration of P-selectin- and VCAM-1-dependent mononuclear cell rolling in early atherosclerotic lesions of apolipoprotein E-deficient mice. Circ Res 84(11):1237-44. [PubMed: 10364560]  [MGI Ref ID J:55953]

Ruth JH; Amin MA; Woods JM; He X; Samuel S; Yi N; Haas CS; Koch AE; Bullard DC. 2005. Accelerated development of arthritis in mice lacking endothelial selectins. Arthritis Res Ther 7(5):R959-70. [PubMed: 16207337]  [MGI Ref ID J:112848]

Scalia R; Armstead VE; Minchenko AG; Lefer AM. 1999. Essential role of P-selectin in the initiation of the inflammatory response induced by hemorrhage and reinfusion. J Exp Med 189(6):931-8. [PubMed: 10075976]  [MGI Ref ID J:110938]

Schneeberger EE; Vu Q; LeBlanc BW; Doerschuk CM. 2000. The accumulation of dendritic cells in the lung is impaired in CD18-/- but not in ICAM-1-/- mutant mice. J Immunol 164(5):2472-8. [PubMed: 10679084]  [MGI Ref ID J:60566]

Singbartl K; Green SA; Ley K. 2000. Blocking P-selectin protects from ischemia/reperfusion-induced acute renal failure. FASEB J 14(1):48-54. [PubMed: 10627279]  [MGI Ref ID J:118770]

Sligh JE Jr; Ballantyne CM; Rich SS; Hawkins HK; Smith CW; Bradley A; Beaudet AL. 1993. Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. Proc Natl Acad Sci U S A 90(18):8529-33. [PubMed: 8104338]  [MGI Ref ID J:14565]

Staite ND; Justen JM; Sly LM; Beaudet AL; Bullard DC. 1996. Inhibition of delayed-type contact hypersensitivity in mice deficient in both E-selectin and P-selectin. Blood 88(8):2973-9. [PubMed: 8874194]  [MGI Ref ID J:36036]

Stark MA; Huo Y; Burcin TL; Morris MA; Olson TS; Ley K. 2005. Phagocytosis of Apoptotic Neutrophils Regulates Granulopoiesis via IL-23 and IL-17. Immunity 22(3):285-94. [PubMed: 15780986]  [MGI Ref ID J:97026]

Steinhoff U; Klemm U; Greiner M; Bordasch K; Kaufmann SH. 1998. Altered intestinal immune system but normal antibacterial resistance in the absence of P-selectin and ICAM-1. J Immunol 160(12):6112-20. [PubMed: 9637528]  [MGI Ref ID J:48760]

Sullivan VV; Hawley AE; Farris DM; Knipp BS; Varga AJ; Wrobleski SK; Thanapron P; Eagleton MJ; Myers DD; Fowlkes JB; Wakefield TW. 2003. Decrease in fibrin content of venous thrombi in selectin-deficient mice. J Surg Res 109(1):1-7. [PubMed: 12591228]  [MGI Ref ID J:119317]

Sun G; Chang WL; Li J; Berney SM; Kimpel D; van der Heyde HC. 2003. Inhibition of platelet adherence to brain microvasculature protects against severe Plasmodium berghei malaria. Infect Immun 71(11):6553-61. [PubMed: 14573677]  [MGI Ref ID J:86276]

Tu L; Poe JC; Kadono T; Venturi GM; Bullard DC; Tedder TF; Steeber DA. 2002. A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. J Immunol 169(4):2034-43. [PubMed: 12165530]  [MGI Ref ID J:120206]

Underhill GH; Kolli KP; Kansas GS. 2003. Complexity within the plasma cell compartment of mice deficient in both E- and P-selectin: implications for plasma cell differentiation. Blood 102(12):4076-83. [PubMed: 12881311]  [MGI Ref ID J:115681]

Underhill GH; Minges-Wols HA; Fornek JL; Witte PL; Kansas GS. 2002. IgG plasma cells display a unique spectrum of leukocyte adhesion and homing molecules. Blood 99(8):2905-12. [PubMed: 11929781]  [MGI Ref ID J:76083]

Vandendries ER; Hamilton JR; Coughlin SR; Furie B; Furie BC. 2007. Par4 is required for platelet thrombus propagation but not fibrin generation in a mouse model of thrombosis. Proc Natl Acad Sci U S A 104(1):288-92. [PubMed: 17190826]  [MGI Ref ID J:118749]

Verdrengh M; Erlandsson-Harris H; Tarkowski A. 2000. Role of selectins in experimental Staphylococcus aureus-induced arthritis. Eur J Immunol 30(6):1606-13. [PubMed: 10898496]  [MGI Ref ID J:62704]

Wang HB; Wang JT; Zhang L; Geng ZH; Xu WL; Xu T; Huo Y; Zhu X; Plow EF; Chen M; Geng JG. 2007. P-selectin primes leukocyte integrin activation during inflammation. Nat Immunol 8(8):882-892. [PubMed: 17632516]  [MGI Ref ID J:123408]

Whalen MJ; Carlos TM; Dixon CE; Robichaud P; Clark RS; Marion DW; Kochanek PM. 2000. Reduced brain edema after traumatic brain injury in mice deficient in P-selectin and intercellular adhesion molecule-1. J Leukoc Biol 67(2):160-8. [PubMed: 10670575]  [MGI Ref ID J:119906]

Wood KC; Hebbel RP; Granger DN. 2004. Endothelial cell P-selectin mediates a proinflammatory and prothrombogenic phenotype in cerebral venules of sickle cell transgenic mice. Am J Physiol Heart Circ Physiol 286(5):H1608-14. [PubMed: 14704223]  [MGI Ref ID J:95603]

Xu Y; Huo Y; Toufektsian MC; Ramos SI; Ma Y; Tejani AD; French BA; Yang Z. 2006. Activated platelets contribute importantly to myocardial reperfusion injury. Am J Physiol Heart Circ Physiol 290(2):H692-9. [PubMed: 16199480]  [MGI Ref ID J:106723]

Yanaba K; Kaburagi Y; Takehara K; Steeber DA; Tedder TF; Sato S. 2003. Relative contributions of selectins and intercellular adhesion molecule-1 to tissue injury induced by immune complex deposition. Am J Pathol 162(5):1463-73. [PubMed: 12707029]  [MGI Ref ID J:113592]

Zanardo RC; Bonder CS; Hwang JM; Andonegui G; Liu L; Vestweber D; Zbytnuik L; Kubes P. 2004. A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions. Blood 104(12):3766-73. [PubMed: 15304396]  [MGI Ref ID J:94829]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Zarbock A; Lowell CA; Ley K. 2007. Spleen tyrosine kinase Syk is necessary for E-selectin-induced alpha(L)beta(2) integrin-mediated rolling on intercellular adhesion molecule-1. Immunity 26(6):773-83. [PubMed: 17543554]  [MGI Ref ID J:123587]

Zarbock A; Singbartl K; Ley K. 2006. Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation. J Clin Invest 116(12):3211-9. [PubMed: 17143330]  [MGI Ref ID J:117354]

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Room Number           AX12

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Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
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