Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S7 via AB2.1 ES cell line Generation N10F37 (14-OCT-08)   Donating Investigator Arthur Beaudet,   Baylor College of Medicine

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Selp^tm1Bay mutation are viable and fertile. Homoyzgous mutant mice show a deficit in leukocyte rolling and delayed neutrophil extravasation in response to intraperitoneal injections of thioglycollate. Neutrophil count is elevated (2-3-fold). Circulating lymphocyte and monocyte counts are normal. 60-70% reduction in neutrophil emigration into the peritoneum during Streptococcus pneumoniae-induced peritonitis is reduced 60-70%. It can be used as model for human leukocyte adhesion deficiency I syndrome (characterized by elevated levels of circulating leukocytes and impaired leukocyte extravasation to sites of inflammation or infection).

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Selp^tm1Bay allele

002285

B6.129S7-Icam1tm1Bay Selptm1Bay/J

View Strains carrying   Selp^tm1Bay     (1 strain)

Strains carrying other alleles of Selp

008441	B6.129S2-Selptm1Hyn Selltm1Hyn/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
003805	B6;129S-Selptm1Hyn Selltm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
002217	B6;129S2-Selptm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J
008442	C.129S2(B6)-Selptm1Hyn Selltm1Hyn/J
008433	C.129S2(B6)-Selptm1Hyn/J

View Strains carrying other alleles of Selp     (8 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Selp^tm1Bay/Selp^tm1Bay

        B6.129S7-Selp^tm1Bay/J

• immune system phenotype
• abnormal T cell physiology (MGI Ref ID J:113536)
  • T lymphocyte migration and accumulation into cutaneous inflammatory regions induced by a combination of IFN-gamma and TNF-alpha is inhibited by about 25%, however IFN-gamma, TNF-alpha, and ConA, has no effect on T cell infiltration

Selp^tm1Bay/Selp^tm1Bay

        either: B6.129S7-Selp^tm1Bay or (involves: 129S7/SvEvBrd * C57BL/6)

• hematopoietic system phenotype
• increased leukocyte cell number (MGI Ref ID J:31374)
  • systemic leukocyte counts are ~9800/ul (~61% neutrophils, ~37% lymphocytes) compared to wild-type counts of ~5660/ul (53% neutrophils, 46% lymphocytes)
• immune system phenotype
• abnormal leukocyte rolling (MGI Ref ID J:31374)
  • after surgery to exteriorize cremaster muscle, mice do not display leukocyte rolling for the first hour, but a significant amount of rolling is detected after 2 hours (~8% rolling leukocyte flux)
  • leukocytes show intermittent interactions with endothelium causing an increase in rolling velocity to ~129 um/second (~35 um/second in wild-type)
  • treatment with Tnfa (tumor necrosis factor alpha) reduces rolling velocity to ~5 um/second
• increased leukocyte cell number (MGI Ref ID J:31374)
  • systemic leukocyte counts are ~9800/ul (~61% neutrophils, ~37% lymphocytes) compared to wild-type counts of ~5660/ul (53% neutrophils, 46% lymphocytes)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Selp^tm1Bay/Selp^tm1Bay

        involves: 129S7/SvEvBrd * C57BL/6J

• immune system phenotype
• abnormal leukocyte migration/homing (MGI Ref ID J:24239)
  • Streptococcus pneumoniae induced peritonitis reduced neutrophil emigration into peritoneum by 61-69% after 2-4 hours
• abnormal spleen morphology (MGI Ref ID J:101979)
  • increase in splenic thrombosis and necrosis in contrast to controls following infection with S. pneumoniae
• eye inflammation (MGI Ref ID J:101979)
  • panophthalmitis observed in 8% of mice following infection with S. pneumoniae
• increased leukocyte cell number (MGI Ref ID J:101979)
  • elevated peripheral leukocyte count in contrast to controls following infection with S. pneumoniae
• increased neutrophil cell number (MGI Ref ID J:101979)
  • mild neutrophilia in contrast to controls following infection with S. pneumoniae
  • 2-3 fold increase in numbers of circulating neutrophils
• increased susceptibility to bacterial infection (MGI Ref ID J:101979)
  • severity and duration of morbidity higher than wild-type after infection with Streptococcus pneumoniae
  • survival rate was 19% in contrast to a 57% rate for wild-type mice
  • decreased ability to clear bacteremia among survivors
• behavior/neurological phenotype
• head tilt (MGI Ref ID J:101979)
  • observed following infection with S. pneumoniae
• hypoactivity (MGI Ref ID J:101979)
  • observed following infection with S. pneumoniae
• piloerection (MGI Ref ID J:101979)
  • observed following infection with S. pneumoniae
• spinning (MGI Ref ID J:101979)
  • observed following infection with S. pneumoniae
• tremors (MGI Ref ID J:101979)
  • observed following infection with S. pneumoniae
• liver/biliary system phenotype
• hepatic necrosis (MGI Ref ID J:101979)
  • higher incidence in contrast to controls following infection with S. pneumoniae
• growth/size phenotype
• weight loss (MGI Ref ID J:101979)
  • observed 48 hours post-infection with S. pneumoniae
• vision/eye phenotype
• eye inflammation (MGI Ref ID J:101979)
  • panophthalmitis observed in 8% of mice following infection with S. pneumoniae
• hematopoietic system phenotype
• abnormal spleen morphology (MGI Ref ID J:101979)
  • increase in splenic thrombosis and necrosis in contrast to controls following infection with S. pneumoniae
• increased leukocyte cell number (MGI Ref ID J:101979)
  • elevated peripheral leukocyte count in contrast to controls following infection with S. pneumoniae
• increased neutrophil cell number (MGI Ref ID J:101979)
  • mild neutrophilia in contrast to controls following infection with S. pneumoniae
  • 2-3 fold increase in numbers of circulating neutrophils
• hearing/vestibular/ear phenotype
• head tilt (MGI Ref ID J:101979)
  • observed following infection with S. pneumoniae

Selp^tm1Bay/Selp^tm1Bay

        D1.129S7(B6J)-Selp^tm1Bay

• skeleton phenotype
• increased susceptibility to induced arthritis (MGI Ref ID J:57086)
  • accelerated incidence of induced arthritis, 23% compared to 2% of DBA/1 mice by 28 days
  • increase in number of affected paws
  • consistently higher arthritis severity scores as determined by inflammation, paw/digit distortion and ankylosis
  • onset of arthritis occurs as early as day 14 with a median onset of 34 days
• immune system phenotype
• abnormal cytokine secretion (MGI Ref ID J:57086)
  • increased levels of IL10 and IL5 measured in stimulated splenocytes
  • decreased levels of IL2 and IL4 measured in stimulated splenocytes
• increased IgG level (MGI Ref ID J:57086)
  • increased levels of serum IgG anti-type II collagen antibody measured in collagen immunized mice as compared to immunized DBA/1 mice
• increased susceptibility to induced arthritis (MGI Ref ID J:57086)
  • accelerated incidence of induced arthritis, 23% compared to 2% of DBA/1 mice by 28 days
  • increase in number of affected paws
  • consistently higher arthritis severity scores as determined by inflammation, paw/digit distortion and ankylosis
  • onset of arthritis occurs as early as day 14 with a median onset of 34 days

Selp^tm1Bay/Selp^tm1Bay

        involves: 129S7/SvEvBrd * C57BL/6

• hematopoietic system phenotype
• increased neutrophil cell number (MGI Ref ID J:112286)
  • circulating neutrophils are increased in mutants and this increases with age of mice
• immune system phenotype
• increased neutrophil cell number (MGI Ref ID J:112286)
  • circulating neutrophils are increased in mutants and this increases with age of mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Selp^tm1Bay related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Selptm1Bay
Allele Name		targeted mutation 1, Baylor College of Medicine
Allele Type		Targeted (knock-out)
Common Name(s)		CD62ELP-; CD62P-; P-; P-selectin-;
Mutation Made By		Arthur Beaudet,   Baylor College of Medicine
Strain of Origin		129S7/SvEvBrd-Hprt1
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1
Gene Symbol and Name		Selp, selectin, platelet
Chromosome		1
Gene Common Name(s)		CD62; CD62P; FLJ45155; GMP140; GRMP; Grmp; LECAM3; MGC124632; P-selectin; PADGEM; PSEL; PSELECT; granulocyte membrane protein;
Molecular Note		A neomycin resistance gene was inserted in the reverse orientation to delete a 4.5kb region containing exons 3 through 5. RT-PCR analysis of lung RNA isolated from homozygous mutant mice showed a transcript 682 base pairs shorter than normal. Immunohistochemical staining showed a lack of encoded protein on both endothelium and platelets. [MGI Ref ID J:24239]

Genotyping

Genotyping Information

Genotyping Protocols

Selp^tm1Bay, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Bullard DC; Qin L; Lorenzo I; Quinlin WM; Doyle NA; Bosse R; Vestweber D; Doerschuk CM; Beaudet AL. 1995. P-selectin/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli [see comments] J Clin Invest 95(4):1782-8. [PubMed: 7535798]  [MGI Ref ID J:24239]

Additional References

Bullard DC; Kunkel EJ; Kubo H; Hicks MJ; Lorenzo I; Doyle NA; Doerschuk CM; Ley K; Beaudet AL. 1996. Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice. J Exp Med 183(5):2329-36. [PubMed: 8642341]  [MGI Ref ID J:33135]

Bullard DC; Mobley JM; Justen JM; Sly LM; Chosay JG; Dunn CJ; Lindsey JR; Beaudet AL; Staite ND. 1999. Acceleration and increased severity of collagen-induced arthritis in P-selectin mutant mice. J Immunol 163(5):2844-9. [PubMed: 10453030]  [MGI Ref ID J:57086]

Doerschuk CM; Quinlan WM; Doyle NA; Bullard DC; Vestweber D; Jones ML; Takei F; Ward PA; Beaudet AL. 1996. The role of P-selectin and ICAM-1 in acute lung injury as determined using blocking antibodies and mutant mice. J Immunol 157(10):4609-14. [PubMed: 8906840]  [MGI Ref ID J:37197]

Huo Y; Schober A; Forlow SB; Smith DF; Hyman MC; Jung S; Littman DR; Weber C; Ley K. 2003. Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E. Nat Med 9(1):61-7. [PubMed: 12483207]  [MGI Ref ID J:81164]

Jung U; Ley K. 1999. Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin. J Immunol 162(11):6755-62. [PubMed: 10352295]  [MGI Ref ID J:55032]

Kamochi M; Kamochi F; Kim YB; Sawh S; Sanders JM; Sarembock I; Green S; Young JS; Ley K; Fu SM; Rose CE Jr. 1999. P-selectin and ICAM-1 mediate endotoxin-induced neutrophil recruitment and injury to the lung and liver. Am J Physiol 277(2 Pt 1):L310-9. [PubMed: 10444525]  [MGI Ref ID J:56844]

Kohm AP; Miller SD. 2003. Role of ICAM-1 and P-selectin expression in the development and effector function of CD4+CD25+regulatory T cells. J Autoimmun 21(3):261-71. [PubMed: 14599851]  [MGI Ref ID J:86435]

Sun G; Chang WL; Li J; Berney SM; Kimpel D; van der Heyde HC. 2003. Inhibition of platelet adherence to brain microvasculature protects against severe Plasmodium berghei malaria. Infect Immun 71(11):6553-61. [PubMed: 14573677]  [MGI Ref ID J:86276]

Tesfaigzi Y; Fischer MJ; Daheshia M; Green FH; De Sanctis GT; Wilder JA. 2002. Bax is crucial for IFN-gamma-induced resolution of allergen-induced mucus cell metaplasia. J Immunol 169(10):5919-25. [PubMed: 12421976]  [MGI Ref ID J:80074]

Selp^tm1Bay related

Baker PJ; DuFour L; Dixon M; Roopenian DC. 2000. Adhesion molecule deficiencies increase Porphyromonas gingivalis-induced alveolar bone loss in mice. Infect Immun 68(6):3103-7. [PubMed: 10816450]  [MGI Ref ID J:62266]

Bartholdy C; Marker O; Thomsen AR. 2000. Migration of activated CD8(+) T lymphocytes to sites of viral infection does not require endothelial selectins. Blood 95(4):1362-9. [PubMed: 10666212]  [MGI Ref ID J:110273]

Bonasio R; Scimone ML; Schaerli P; Grabie N; Lichtman AH; von Andrian UH. 2006. Clonal deletion of thymocytes by circulating dendritic cells homing to the thymus. Nat Immunol 7(10):1092-100. [PubMed: 16951687]  [MGI Ref ID J:112629]

Bonig H; Priestley GV; Oehler V; Papayannopoulou T. 2007. Hematopoietic progenitor cells (HPC) from mobilized peripheral blood display enhanced migration and marrow homing compared to steady-state bone marrow HPC. Exp Hematol 35(2):326-34. [PubMed: 17258081]  [MGI Ref ID J:123189]

Briaud SA; Ding ZM; Michael LH; Entman ML; Daniel S; Ballantyne CM. 2001. Leukocyte trafficking and myocardial reperfusion injury in ICAM-1/P-selectin-knockout mice. Am J Physiol Heart Circ Physiol 280(1):H60-7. [PubMed: 11123218]  [MGI Ref ID J:68056]

Broide DH; Humber D; Sullivan S; Sriramarao P. 1998. Inhibition of eosinophil rolling and recruitment in P-selectin- and intracellular adhesion molecule-1-deficient mice [published erratum appears in Blood 1998 Jul 1;92(1):343] Blood 91(8):2847-56. [PubMed: 9531595]  [MGI Ref ID J:47461]

Broide DH; Sullivan S; Gifford T; Sriramarao P. 1998. Inhibition of pulmonary eosinophilia in P-selectin- and ICAM-1-deficient mice. Am J Respir Cell Mol Biol 18(2):218-25. [PubMed: 9476909]  [MGI Ref ID J:114264]

Bullard DC; Kunkel EJ; Kubo H; Hicks MJ; Lorenzo I; Doyle NA; Doerschuk CM; Ley K; Beaudet AL. 1996. Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice. J Exp Med 183(5):2329-36. [PubMed: 8642341]  [MGI Ref ID J:33135]

Bullard DC; Mobley JM; Justen JM; Sly LM; Chosay JG; Dunn CJ; Lindsey JR; Beaudet AL; Staite ND. 1999. Acceleration and increased severity of collagen-induced arthritis in P-selectin mutant mice. J Immunol 163(5):2844-9. [PubMed: 10453030]  [MGI Ref ID J:57086]

Carlow DA; Ziltener HJ. 2006. CD43 deficiency has no impact in competitive in vivo assays of neutrophil or activated T cell recruitment efficiency. J Immunol 177(9):6450-9. [PubMed: 17056577]  [MGI Ref ID J:140506]

Carrithers MD; Visintin I; Viret C; Janeway CS Jr. 2002. Role of genetic background in P selectin-dependent immune surveillance of the central nervous system. J Neuroimmunol 129(1-2):51-7. [PubMed: 12161020]  [MGI Ref ID J:102957]

Collins RG; Jung U; Ramirez M; Bullard DC; Hicks MJ; Smith CW; Ley K; Beaudet AL. 2001. Dermal and pulmonary inflammatory disease in E-selectin and P-selectin double-null mice is reduced in triple-selectin-null mice. Blood 98(3):727-35. [PubMed: 11468173]  [MGI Ref ID J:70682]

Collins RG; Velji R; Guevara NV; Hicks MJ; Chan L; Beaudet AL. 2000. P-Selectin or intercellular adhesion molecule (ICAM)-1 deficiency substantially protects against atherosclerosis in apolipoprotein E-deficient mice. J Exp Med 191(1):189-94. [PubMed: 10620617]  [MGI Ref ID J:59249]

Curtis JL; Sonstein J; Craig RA; Todt JC; Knibbs RN; Polak T; Bullard DC; Stoolman LM. 2002. Subset-specific reductions in lung lymphocyte accumulation following intratracheal antigen challenge in endothelial selectin-deficient mice. J Immunol 169(5):2570-9. [PubMed: 12193727]  [MGI Ref ID J:120687]

Doerschuk CM; Quinlan WM; Doyle NA; Bullard DC; Vestweber D; Jones ML; Takei F; Ward PA; Beaudet AL. 1996. The role of P-selectin and ICAM-1 in acute lung injury as determined using blocking antibodies and mutant mice. J Immunol 157(10):4609-14. [PubMed: 8906840]  [MGI Ref ID J:37197]

Eppihimer MJ; Russell J; Anderson DC; Wolitzky BA; Granger DN. 1997. Endothelial cell adhesion molecule expression in gene-targeted mice. Am J Physiol 273(4 Pt 2):H1903-8. [PubMed: 9362259]  [MGI Ref ID J:43907]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Evangelista V; Manarini S; Coller BS; Smyth SS. 2003. Role of P-selectin, beta2-integrins, and Src tyrosine kinases in mouse neutrophil-platelet adhesion. J Thromb Haemost 1(5):1048-54. [PubMed: 12871376]  [MGI Ref ID J:104023]

Falati S; Liu Q; Gross P; Merrill-Skoloff G; Chou J; Vandendries E; Celi A; Croce K; Furie BC; Furie B. 2003. Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin. J Exp Med 197(11):1585-98. [PubMed: 12782720]  [MGI Ref ID J:83734]

Forlow SB; Foley PL; Ley K. 2002. Severely reduced neutrophil adhesion and impaired host defense against fecal and commensal bacteria in CD18-/-P-selectin-/- double null mice. FASEB J 16(12):1488-96. [PubMed: 12374771]  [MGI Ref ID J:120011]

Forlow SB; Ley K. 2001. Selectin-independent leukocyte rolling and adhesion in mice deficient in E-, P-, and L-selectin and ICAM-1. Am J Physiol Heart Circ Physiol 280(2):H634-41. [PubMed: 11158961]  [MGI Ref ID J:68044]

Forlow SB; White EJ; Thomas KL; Bagby GJ; Foley PL; Ley K. 2002. T cell requirement for development of chronic ulcerative dermatitis in e- and p-selectin-deficient mice. J Immunol 169(9):4797-804. [PubMed: 12391189]  [MGI Ref ID J:79774]

Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58. [PubMed: 17456770]  [MGI Ref ID J:121083]

Graciano AL; Bryant DD; White DJ; Horton J; Bowles NE; Giroir BP. 2001. Targeted disruption of ICAM-1, P-selectin genes improves cardiac function and survival in TNF-alpha transgenic mice. Am J Physiol Heart Circ Physiol 280(4):H1464-71. [PubMed: 11247755]  [MGI Ref ID J:108278]

Gregory JL; Morand EF; McKeown SJ; Ralph JA; Hall P; Yang YH; McColl SR; Hickey MJ. 2006. Macrophage migration inhibitory factor induces macrophage recruitment via CC chemokine ligand 2. J Immunol 177(11):8072-9. [PubMed: 17114481]  [MGI Ref ID J:140686]

Hafezi-Moghadam A; Thomas KL; Prorock AJ; Huo Y; Ley K. 2001. L-selectin shedding regulates leukocyte recruitment. J Exp Med 193(7):863-72. [PubMed: 11283159]  [MGI Ref ID J:120579]

He X; Schoeb TR; Panoskaltsis-Mortari A; Zinn KR; Kesterson RA; Zhang J; Samuel S; Hicks MJ; Hickey MJ; Bullard DC. 2006. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. J Immunol 177(12):8748-56. [PubMed: 17142777]  [MGI Ref ID J:127199]

Hickey MJ; Bullard DC; Issekutz A; James WG. 2002. Leukocyte-endothelial cell interactions are enhanced in dermal postcapillary venules of MRL/fas(lpr) (lupus-prone) mice: roles of P- and E-selectin. J Immunol 168(9):4728-36. [PubMed: 11971023]  [MGI Ref ID J:126009]

Hickey MJ; Granger DN; Kubes P. 1999. Molecular mechanisms underlying IL-4-induced leukocyte recruitment in vivo: a critical role for the alpha 4 integrin. J Immunol 163(6):3441-8. [PubMed: 10477616]  [MGI Ref ID J:119200]

Hicks AE; Abbitt KB; Dodd P; Ridger VC; Hellewell PG; Norman KE. 2005. The anti-inflammatory effects of a selectin ligand mimetic, TBC-1269, are not a result of competitive inhibition of leukocyte rolling in vivo. J Leukoc Biol 77(1):59-66. [PubMed: 15466915]  [MGI Ref ID J:95144]

Horikawa M; Fujimoto M; Hasegawa M; Matsushita T; Hamaguchi Y; Kawasuji A; Matsushita Y; Fujita T; Ogawa F; Takehara K; Steeber DA; Sato S. 2006. E- and P-selectins synergistically inhibit bleomycin-induced pulmonary fibrosis. Am J Pathol 169(3):740-9. [PubMed: 16936251]  [MGI Ref ID J:112363]

Huo Y; Schober A; Forlow SB; Smith DF; Hyman MC; Jung S; Littman DR; Weber C; Ley K. 2003. Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E. Nat Med 9(1):61-7. [PubMed: 12483207]  [MGI Ref ID J:81164]

James WG; Bullard DC; Hickey MJ. 2003. Critical role of the alpha 4 integrin/VCAM-1 pathway in cerebral leukocyte trafficking in lupus-prone MRL/fas(lpr) mice. J Immunol 170(1):520-7. [PubMed: 12496439]  [MGI Ref ID J:127282]

Jones SP; Trocha SD; Strange MB; Granger DN; Kevil CG; Bullard DC; Lefer DJ. 2000. Leukocyte and endothelial cell adhesion molecules in a chronic murine model of myocardial reperfusion injury Am J Physiol Heart Circ Physiol 279(5):H2196-201. [PubMed: 11045953]  [MGI Ref ID J:65658]

Jung U; Ley K. 1999. Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin. J Immunol 162(11):6755-62. [PubMed: 10352295]  [MGI Ref ID J:55032]

Jung U; Ramos CL; Bullard DC; Ley K. 1998. Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion. Am J Physiol 274(5 Pt 2):H1785-91. [PubMed: 9612391]  [MGI Ref ID J:47723]

Kamochi M; Kamochi F; Kim YB; Sawh S; Sanders JM; Sarembock I; Green S; Young JS; Ley K; Fu SM; Rose CE Jr. 1999. P-selectin and ICAM-1 mediate endotoxin-induced neutrophil recruitment and injury to the lung and liver. Am J Physiol 277(2 Pt 1):L310-9. [PubMed: 10444525]  [MGI Ref ID J:56844]

Kerfoot SM; Long EM; Hickey MJ; Andonegui G; Lapointe BM; Zanardo RC; Bonder C; James WG; Robbins SM; Kubes P. 2004. TLR4 contributes to disease-inducing mechanisms resulting in central nervous system autoimmune disease. J Immunol 173(11):7070-7. [PubMed: 15557205]  [MGI Ref ID J:94363]

Kerfoot SM; Norman MU; Lapointe BM; Bonder CS; Zbytnuik L; Kubes P. 2006. Reevaluation of P-selectin and alpha 4 integrin as targets for the treatment of experimental autoimmune encephalomyelitis. J Immunol 176(10):6225-34. [PubMed: 16670333]  [MGI Ref ID J:131757]

Kim MH; Carter PR; Harris NR. 2007. P-selectin-mediated adhesion impairs endothelium-dependent arteriolar dilation in hypercholesterolemic mice. Am J Physiol Heart Circ Physiol 292(1):H632-8. [PubMed: 16980348]  [MGI Ref ID J:119942]

Kohm AP; Miller SD. 2003. Role of ICAM-1 and P-selectin expression in the development and effector function of CD4+CD25+regulatory T cells. J Autoimmun 21(3):261-71. [PubMed: 14599851]  [MGI Ref ID J:86435]

Komura K; Hasegawa M; Hamaguchi Y; Saito E; Kaburagi Y; Yanaba K; Kawara S; Takehara K; Seki M; Steeber DA; Tedder TF; Sato S. 2003. Ultraviolet light exposure suppresses contact hypersensitivity by abrogating endothelial intercellular adhesion molecule-1 up-regulation at the elicitation site. J Immunol 171(6):2855-62. [PubMed: 12960307]  [MGI Ref ID J:85377]

Kulidjian AA; Issekutz AC; Issekutz TB. 2002. Differential role of E-selectin and P-selectin in T lymphocyte migration to cutaneous inflammatory reactions induced by cytokines. Int Immunol 14(7):751-60. [PubMed: 12096034]  [MGI Ref ID J:113536]

Kuligowski MP; Kitching AR; Hickey MJ. 2006. Leukocyte recruitment to the inflamed glomerulus: a critical role for platelet-derived P-selectin in the absence of rolling. J Immunol 176(11):6991-9. [PubMed: 16709860]  [MGI Ref ID J:131787]

Kunkel EJ; Chomas JE; Ley K. 1998. Role of primary and secondary capture for leukocyte accumulation in vivo. Circ Res 82(1):30-8. [PubMed: 9440702]  [MGI Ref ID J:114235]

Kunkel EJ; Jung U; Bullard DC; Norman KE; Wolitzky BA; Vestweber D; Beaudet AL; Ley K. 1996. Absence of trauma-induced leukocyte rolling in mice deficient in both P-selectin and intercellular adhesion molecule 1. J Exp Med 183(1):57-65. [PubMed: 8551244]  [MGI Ref ID J:31374]

Kupatt C; Wichels R; Horstkotte J; Krombach F; Habazettl H; Boekstegers P. 2002. Molecular mechanisms of platelet-mediated leukocyte recruitment during myocardial reperfusion. J Leukoc Biol 72(3):455-61. [PubMed: 12223512]  [MGI Ref ID J:124451]

Larbi KY; Dangerfield JP; Culley FJ; Marshall D; Haskard DO; Jose PJ; Williams TJ; Nourshargh S. 2003. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses. J Leukoc Biol 73(1):65-73. [PubMed: 12525563]  [MGI Ref ID J:120684]

Laubli H; Stevenson JL; Varki A; Varki NM; Borsig L. 2006. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res 66(3):1536-42. [PubMed: 16452210]  [MGI Ref ID J:106671]

Lawson JA; Burns AR; Farhood A; Lynn Bajt M; Collins RG; Smith CW; Jaeschke H. 2000. Pathophysiologic importance of E- and L-selectin for neutrophil-induced liver injury during endotoxemia in mice. Hepatology 32(5):990-8. [PubMed: 11050049]  [MGI Ref ID J:106550]

Li Z; Burns AR; Rumbaut RE; Smith CW. 2007. {gamma}{delta} T Cells Are Necessary for Platelet and Neutrophil Accumulation in Limbal Vessels and Efficient Epithelial Repair after Corneal Abrasion. Am J Pathol 171(3):838-845. [PubMed: 17675580]  [MGI Ref ID J:124350]

Li Z; Burns AR; Smith CW. 2006. Two waves of neutrophil emigration in response to corneal epithelial abrasion: distinct adhesion molecule requirements. Invest Ophthalmol Vis Sci 47(5):1947-55. [PubMed: 16639002]  [MGI Ref ID J:108504]

Li Z; Rumbaut RE; Burns AR; Smith CW. 2006. Platelet response to corneal abrasion is necessary for acute inflammation and efficient re-epithelialization. Invest Ophthalmol Vis Sci 47(11):4794-802. [PubMed: 17065490]  [MGI Ref ID J:123092]

Lukacs NW; John A; Berlin A; Bullard DC; Knibbs R; Stoolman LM. 2002. E- and P-selectins are essential for the development of cockroach allergen-induced airway responses. J Immunol 169(4):2120-5. [PubMed: 12165540]  [MGI Ref ID J:120695]

Manka D; Collins RG; Ley K; Beaudet AL; Sarembock IJ. 2001. Absence of p-selectin, but not intercellular adhesion molecule-1, attenuates neointimal growth after arterial injury in apolipoprotein e-deficient mice. Circulation 103(7):1000-5. [PubMed: 11181476]  [MGI Ref ID J:103301]

Mizgerd JP; Bullard DC; Hicks MJ; Beaudet AL; Doerschuk CM. 1999. Chronic inflammatory disease alters adhesion molecule requirements for acute neutrophil emigration in mouse skin. J Immunol 162(9):5444-8. [PubMed: 10228023]  [MGI Ref ID J:112286]

Mori M; Salter JW; Vowinkel T; Krieglstein CF; Stokes KY; Granger DN. 2005. Molecular determinants of the prothrombogenic phenotype assumed by inflamed colonic venules. Am J Physiol Gastrointest Liver Physiol 288(5):G920-6. [PubMed: 15550557]  [MGI Ref ID J:98225]

Munoz FM; Hawkins EP; Bullard DC; Beaudet AL; Kaplan SL. 1997. Host defense against systemic infection with Streptococcus pneumoniae is impaired in E-, P-, and E-/P-selectin-deficient mice. J Clin Invest 100(8):2099-106. [PubMed: 9329976]  [MGI Ref ID J:101979]

Myers D Jr; Farris D; Hawley A; Wrobleski S; Chapman A; Stoolman L; Knibbs R; Strieter R; Wakefield T. 2002. Selectins influence thrombosis in a mouse model of experimental deep venous thrombosis. J Surg Res 108(2):212-21. [PubMed: 12505044]  [MGI Ref ID J:103110]

Norman KE; Katopodis AG; Thoma G; Kolbinger F; Hicks AE; Cotter MJ; Pockley AG; Hellewell PG. 2000. P-selectin glycoprotein ligand-1 supports rolling on E- and P-selectin in vivo Blood 96(10):3585-91. [PubMed: 11071658]  [MGI Ref ID J:65810]

Osterholzer JJ; Ames T; Polak T; Sonstein J; Moore BB; Chensue SW; Toews GB; Curtis JL. 2005. CCR2 and CCR6, but not endothelial selectins, mediate the accumulation of immature dendritic cells within the lungs of mice in response to particulate antigen. J Immunol 175(2):874-83. [PubMed: 16002685]  [MGI Ref ID J:100694]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Pelletier SD; Hong DS; Hu Y; Liu Y; Li S. 2004. Lack of the adhesion molecules P-selectin and intercellular adhesion molecule-1 accelerate the development of BCR/ABL-induced chronic myeloid leukemia-like myeloproliferative disease in mice. Blood 104(7):2163-71. [PubMed: 15213099]  [MGI Ref ID J:93772]

Pierangeli SS; Espinola RG; Liu X; Harris EN. 2001. Thrombogenic effects of antiphospholipid antibodies are mediated by intercellular cell adhesion molecule-1, vascular cell adhesion molecule-1, and P-selectin. Circ Res 88(2):245-50. [PubMed: 11157679]  [MGI Ref ID J:115602]

Puri KD; Doggett TA; Huang CY; Douangpanya J; Hayflick JS; Turner M; Penninger J; Diacovo TG. 2005. The role of endothelial PI3Kgamma activity in neutrophil trafficking. Blood 106(1):150-7. [PubMed: 15769890]  [MGI Ref ID J:107472]

Raisanen-Sokolowski A; Glysing-Jensen T; Russell ME. 1999. Donor and recipient contributions of ICAM-1 and P-selectin in parenchymal rejection and graft arteriosclerosis: insights from double knockout mice. J Heart Lung Transplant 18(8):735-43. [PubMed: 10512519]  [MGI Ref ID J:59743]

Ramos CL; Huo Y; Jung U; Ghosh S; Manka DR; Sarembock IJ; Ley K. 1999. Direct demonstration of P-selectin- and VCAM-1-dependent mononuclear cell rolling in early atherosclerotic lesions of apolipoprotein E-deficient mice. Circ Res 84(11):1237-44. [PubMed: 10364560]  [MGI Ref ID J:55953]

Ruth JH; Amin MA; Woods JM; He X; Samuel S; Yi N; Haas CS; Koch AE; Bullard DC. 2005. Accelerated development of arthritis in mice lacking endothelial selectins. Arthritis Res Ther 7(5):R959-70. [PubMed: 16207337]  [MGI Ref ID J:112848]

Scalia R; Armstead VE; Minchenko AG; Lefer AM. 1999. Essential role of P-selectin in the initiation of the inflammatory response induced by hemorrhage and reinfusion. J Exp Med 189(6):931-8. [PubMed: 10075976]  [MGI Ref ID J:110938]

Schneeberger EE; Vu Q; LeBlanc BW; Doerschuk CM. 2000. The accumulation of dendritic cells in the lung is impaired in CD18-/- but not in ICAM-1-/- mutant mice. J Immunol 164(5):2472-8. [PubMed: 10679084]  [MGI Ref ID J:60566]

Singbartl K; Green SA; Ley K. 2000. Blocking P-selectin protects from ischemia/reperfusion-induced acute renal failure. FASEB J 14(1):48-54. [PubMed: 10627279]  [MGI Ref ID J:118770]

Sligh JE Jr; Ballantyne CM; Rich SS; Hawkins HK; Smith CW; Bradley A; Beaudet AL. 1993. Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. Proc Natl Acad Sci U S A 90(18):8529-33. [PubMed: 8104338]  [MGI Ref ID J:14565]

Staite ND; Justen JM; Sly LM; Beaudet AL; Bullard DC. 1996. Inhibition of delayed-type contact hypersensitivity in mice deficient in both E-selectin and P-selectin. Blood 88(8):2973-9. [PubMed: 8874194]  [MGI Ref ID J:36036]

Stark MA; Huo Y; Burcin TL; Morris MA; Olson TS; Ley K. 2005. Phagocytosis of Apoptotic Neutrophils Regulates Granulopoiesis via IL-23 and IL-17. Immunity 22(3):285-94. [PubMed: 15780986]  [MGI Ref ID J:97026]

Steinhoff U; Klemm U; Greiner M; Bordasch K; Kaufmann SH. 1998. Altered intestinal immune system but normal antibacterial resistance in the absence of P-selectin and ICAM-1. J Immunol 160(12):6112-20. [PubMed: 9637528]  [MGI Ref ID J:48760]

Sullivan VV; Hawley AE; Farris DM; Knipp BS; Varga AJ; Wrobleski SK; Thanapron P; Eagleton MJ; Myers DD; Fowlkes JB; Wakefield TW. 2003. Decrease in fibrin content of venous thrombi in selectin-deficient mice. J Surg Res 109(1):1-7. [PubMed: 12591228]  [MGI Ref ID J:119317]

Sun G; Chang WL; Li J; Berney SM; Kimpel D; van der Heyde HC. 2003. Inhibition of platelet adherence to brain microvasculature protects against severe Plasmodium berghei malaria. Infect Immun 71(11):6553-61. [PubMed: 14573677]  [MGI Ref ID J:86276]

Tu L; Poe JC; Kadono T; Venturi GM; Bullard DC; Tedder TF; Steeber DA. 2002. A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. J Immunol 169(4):2034-43. [PubMed: 12165530]  [MGI Ref ID J:120206]

Underhill GH; Kolli KP; Kansas GS. 2003. Complexity within the plasma cell compartment of mice deficient in both E- and P-selectin: implications for plasma cell differentiation. Blood 102(12):4076-83. [PubMed: 12881311]  [MGI Ref ID J:115681]

Underhill GH; Minges-Wols HA; Fornek JL; Witte PL; Kansas GS. 2002. IgG plasma cells display a unique spectrum of leukocyte adhesion and homing molecules. Blood 99(8):2905-12. [PubMed: 11929781]  [MGI Ref ID J:76083]

Vandendries ER; Hamilton JR; Coughlin SR; Furie B; Furie BC. 2007. Par4 is required for platelet thrombus propagation but not fibrin generation in a mouse model of thrombosis. Proc Natl Acad Sci U S A 104(1):288-92. [PubMed: 17190826]  [MGI Ref ID J:118749]

Verdrengh M; Erlandsson-Harris H; Tarkowski A. 2000. Role of selectins in experimental Staphylococcus aureus-induced arthritis. Eur J Immunol 30(6):1606-13. [PubMed: 10898496]  [MGI Ref ID J:62704]

Wang HB; Wang JT; Zhang L; Geng ZH; Xu WL; Xu T; Huo Y; Zhu X; Plow EF; Chen M; Geng JG. 2007. P-selectin primes leukocyte integrin activation during inflammation. Nat Immunol 8(8):882-892. [PubMed: 17632516]  [MGI Ref ID J:123408]

Whalen MJ; Carlos TM; Dixon CE; Robichaud P; Clark RS; Marion DW; Kochanek PM. 2000. Reduced brain edema after traumatic brain injury in mice deficient in P-selectin and intercellular adhesion molecule-1. J Leukoc Biol 67(2):160-8. [PubMed: 10670575]  [MGI Ref ID J:119906]

Wood KC; Hebbel RP; Granger DN. 2004. Endothelial cell P-selectin mediates a proinflammatory and prothrombogenic phenotype in cerebral venules of sickle cell transgenic mice. Am J Physiol Heart Circ Physiol 286(5):H1608-14. [PubMed: 14704223]  [MGI Ref ID J:95603]

Xu Y; Huo Y; Toufektsian MC; Ramos SI; Ma Y; Tejani AD; French BA; Yang Z. 2006. Activated platelets contribute importantly to myocardial reperfusion injury. Am J Physiol Heart Circ Physiol 290(2):H692-9. [PubMed: 16199480]  [MGI Ref ID J:106723]

Yanaba K; Kaburagi Y; Takehara K; Steeber DA; Tedder TF; Sato S. 2003. Relative contributions of selectins and intercellular adhesion molecule-1 to tissue injury induced by immune complex deposition. Am J Pathol 162(5):1463-73. [PubMed: 12707029]  [MGI Ref ID J:113592]

Zanardo RC; Bonder CS; Hwang JM; Andonegui G; Liu L; Vestweber D; Zbytnuik L; Kubes P. 2004. A down-regulatable E-selectin ligand is functionally important for PSGL-1-independent leukocyte-endothelial cell interactions. Blood 104(12):3766-73. [PubMed: 15304396]  [MGI Ref ID J:94829]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Zarbock A; Lowell CA; Ley K. 2007. Spleen tyrosine kinase Syk is necessary for E-selectin-induced alpha(L)beta(2) integrin-mediated rolling on intercellular adhesion molecule-1. Immunity 26(6):773-83. [PubMed: 17543554]  [MGI Ref ID J:123587]

Zarbock A; Singbartl K; Ley K. 2006. Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation. J Clin Invest 116(12):3211-9. [PubMed: 17143330]  [MGI Ref ID J:117354]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.