Strain Name: |
129-Gt(ROSA)26Sor/J |
|---|---|
Stock Number: |
002292 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
129-Gt(ROSA)26Sor (Changed: 15-DEC-04
) |
|
129/Sv-Gtrosa26 (Changed: 15-DEC-04
) | |
| Genes & Alleles | Gt(ROSA)26Sor; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Gene Trap Type JAX® GEMM® Strain - Mutant Strain Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Generation N?+2F17 (08-JAN-08) Appearance
white-bellied agouti
Related Genotype: Aw/AwStrain Description
Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse. Formerly named TgR(ROSA26)26Sor.Strain Development
This strain was developed in the laboratory of Dr. Philippe Soriano at the Fred Hutchinson Cancer Center, Seattle, WA. This mutant was made by a retroviral insertion of the beta-galactosidase gene (and accompanying splice acceptor and neo sequences) into ES cells. The construct was inserted in reverse orientation in the retroviral vector pGen-.
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sor | ||
|---|---|---|---|
| Allele Name | gene trap ROSA 26, Philippe Soriano | ||
| Common Name(s) | ROSA26; ROSA26-lacZ; Rosa26lacZ; TgR(ROSA26)26Sor; gtrosa26; | ||
| Mutation Made By | Philippe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129 x C57BL/6J | ||
| Site of Expression | All tissues of the developing embryo and in most tissues of the adult mouse. | ||
| General Note | No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells. | ||
| Molecular Note | Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse. | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 002448 129S1/SvImJ | (approximate) | |
| 129S1/SvImJ mice (Stock No. 002448) may be used as controls. This strain only provides an approximate match to this 129 strain. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sor
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
002192 B6.129S7-Gt(ROSA)26Sor/J 002073 B6;129S-Gt(ROSA)26Sor/J 002955 C.129S7-Gt(ROSA)26Sor/J 005420 C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
New 129 Nomenclature Bulletin
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Developmental Biology Research
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
lacZ Expression
References
Selected Reference(s)
Additional ReferencesFriedrich G; Soriano P. 1991. Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev 5(9):1513-23. [PubMed: 1653172] [J:79478]
Price and Supply Information
| Strain Name: | 129-Gt(ROSA)26Sor/J |
| Stock Number: | 002292 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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