Strain Name:

DBA/1LacJ-Scd1ab-2J/J

Stock Number:

002304

Availability:

Repository-Cryopreserved

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse

Description
The overall appearance of mice homozygous for either the Scd1ab-J allele or the Scd1ab-2J allele (Stock No. 002304 or 005956) includes slightly hunched posture, dry, scaly skin, thin fur sometimes detectable by 7 days of age, and small eyes with encrusted eyelids stuck shut. They have hypoplasia of the sebaceous glands and other hair follicle abnormalities that result in scarring alopecia. These mice have a paucity of adipose tissue, thin subcutis, and a distinctive odor. Hepatic cholesterol ester and triglyceride synthesis has been shown to be deficient in Scd1ab-J homozygotes and could not be restored through diet. Early studies of skin lipids in the original asebia mutant (Scd1ab) revealed a deficiency in wax esters, wax diesters, and sterols esterified with very long-chain fatty acids along with an increase in free sterols. (Wilkinson and Darasek, 1966; Sundberg et al., 1994; Zheng et al. 1999; Miyazaki et al., 2000; Sundberg et al., 2000)

Mice homozygous for the Scd1ab-2J allele have greater dermal inflammatory infiltrate in the skin and thicker epidermis than do Scd1ab-J mice. Scd1ab-2J homozygotes show an increased transepidermal water loss and corresponding ad libitum intake of water that is not seen in Scd1ab-J homozygotes. For a thorough description see Sundberg et al., 2000 and Sundberg, 1994.

Development
The Scd1ab-2J allele arose in 1993 on the DBA/1LacJ background at The Jackson Laboratory. The mutation was maintained by sibling mating at each generation.

Related Strains

Strains carrying   Scd1ab-2J allele
005956   B6;D1Lac-Scd1ab-2J/J
View Strains carrying   Scd1ab-2J     (1 strain)

Strains carrying other alleles of Scd1
006201   B6.129-Scd1tm1Ntam/J
View Strains carrying other alleles of Scd1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Scd1ab-2J/Scd1ab-2J

        DBA/1LacJ-Scd1ab-2J/J
  • skin/coat/nails phenotype
  • abnormal hair follicle morphology (MGI Ref ID J:62699)
    • hair follicle bulbar histology is distorted compared to in wild-type mice
    • hair shafts rupture through the base of the hair follicle and induce a foreign body granuloma in the dermal stroma resulting in dermal scarring and eventually scarring of the hair follicle
    • while few follicles continue to cycle, with each cycle there is a progressive loss of individual follicles
    • abnormal hair follicle development (MGI Ref ID J:62699)
      • follicles stay in anagen longer than in wild-type mice
    • abnormal hair follicle root sheath (MGI Ref ID J:62699)
      • the inner root sheath is retained beyond the midfollicle into the outer piliary canal unlike in wild-type mice
    • enlarged hair follicles (MGI Ref ID J:62699)
      • during the first anagen stage the hair follicles are unusually long and extend at a sharp angle into the deep subcutis unlike in wild-type mice
  • abnormal hair shaft morphology (MGI Ref ID J:62699)
    • hair shafts rupture through the base of the hair follicle and induce a foreign body granuloma in the dermal stroma resulting in dermal scarring and eventually scarring of the hair follicle
  • abnormal skin morphology (MGI Ref ID J:62699)
    • mice exhibit thicker than normal epidermis, Malphigian layer and stratum corneum
    • abnormal meibomian gland morphology (MGI Ref ID J:62699)
      • small
    • acanthosis (MGI Ref ID J:62699)
    • hyperkeratosis (MGI Ref ID J:62699)
      • mice exhibit varying degrees of orthokeratotic hyperkeratosis
    • parakeratosis (MGI Ref ID J:62699)
      • in focal areas
    • sebaceous gland atrophy (MGI Ref ID J:62699)
      • at day 6 mice have very small sebaceous glands compared to in wild-type mice
      • mice have small meibomian, perianal and cerimous glands
      • however, preputial, clitoral, lingual, Harderian and lacrimal glands are normal
    • thick dermal layer (MGI Ref ID J:62699)
  • dry skin (MGI Ref ID J:62699)
  • flaky skin (MGI Ref ID J:62699)
    • moderate white flaking is observed when hair is removed unlike in wild-type mice
  • impaired skin barrier function (MGI Ref ID J:62699)
    • transepidermal water loss is increased 4.5-fold compared to in wild-type mice
  • matted coat (MGI Ref ID J:62699)
  • scaly skin (MGI Ref ID J:62699)
  • sparse hair (MGI Ref ID J:62699)
  • behavior/neurological phenotype
  • hunched posture (MGI Ref ID J:62699)
  • increased drinking behavior (MGI Ref ID J:62699)
  • homeostasis/metabolism phenotype
  • abnormal lipid level (MGI Ref ID J:62699)
    • mice exhibit a reduction in skin surface lipids such as sterol esters and cholesterol with a total loss of diol esters unlike in wild-type mice
  • impaired skin barrier function (MGI Ref ID J:62699)
    • transepidermal water loss is increased 4.5-fold compared to in wild-type mice
  • immune system phenotype
  • granulomatous inflammation (MGI Ref ID J:62699)
    • hair shafts rupture through the base of the hair follicle and induce a foreign body granuloma in the dermal stroma resulting in dermal scarring and eventually scarring of the hair follicle
  • renal/urinary system phenotype
  • polyuria (MGI Ref ID J:62699)
    • urine output is increased by 2% compared to in wild-type mice
  • endocrine/exocrine gland phenotype
  • abnormal meibomian gland morphology (MGI Ref ID J:62699)
    • small
  • sebaceous gland atrophy (MGI Ref ID J:62699)
    • at day 6 mice have very small sebaceous glands compared to in wild-type mice
    • mice have small meibomian, perianal and cerimous glands
    • however, preputial, clitoral, lingual, Harderian and lacrimal glands are normal
  • vision/eye phenotype
  • abnormal meibomian gland morphology (MGI Ref ID J:62699)
    • small
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Scd1ab-2J related

Cardiovascular Research
Hypotriglyceridemia

Dermatology Research
Skin and Hair Texture Defects

Internal/Organ Research
Adipose Defects

Metabolism Research
Lipid Metabolism

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Scd1ab-2J
Allele Name asebia 2 Jackson
Allele Type Spontaneous
Strain of OriginDBA/1LacJ
Gene Symbol and Name Scd1, stearoyl-Coenzyme A desaturase 1
Chromosome 19
Gene Common Name(s) AA589638; AI265570; SCD; Scd-1; ab; asebia; expressed sequence AA589638; expressed sequence AI265570; stearoyl-CoA desaturase;
Molecular Note An 18 bp deletion in the exon 2/intron 2 boundary. [MGI Ref ID J:58283]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Ntambi JM; Buhrow SA; Kaestner KH; Christy RJ; Sibley E; Kelly TJ Jr; Lane MD. 1988. Differentiation-induced gene expression in 3T3-L1 preadipocytes. Characterization of a differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem 263(33):17291-300. [PubMed: 2903162]  [MGI Ref ID J:9448]

Sundberg JP; Boggess D; Sundberg BA; Eilertsen K; Parimoo S; Filippi M; Stenn K. 2000. Asebia-2J (Scd1(ab2J)): a new allele and a model for scarring alopecia. Am J Pathol 156(6):2067-75. [PubMed: 10854228]  [MGI Ref ID J:62699]

Wilkinson DI; Karasek MA. 1966. Skin lipids of a normal and mutant (asebic) mouse strain. J Invest Dermatol 47(5):449-55. [PubMed: 5924301]  [MGI Ref ID J:5013]

Zheng Y; Eilertsen KJ; Ge L; Zhang L; Sundberg JP; Prouty SM; Stenn KS; Parimoo S. 1999. Scd1 is expressed in sebaceous glands and is disrupted in the asebia mouse [letter] Nat Genet 23(3):268-70. [PubMed: 10545940]  [MGI Ref ID J:58283]

Scd1ab-2J related

MacDonald ML; Singaraja RR; Bissada N; Ruddle P; Watts R; Karasinska JM; Gibson WT; Fievet C; Vance JE; Staels B; Hayden MR. 2008. Absence of stearoyl-CoA desaturase-1 ameliorates features of the metabolic syndrome in LDLR-deficient mice. J Lipid Res 49(1):217-29. [PubMed: 17960025]  [MGI Ref ID J:130278]

Sundberg JP; Boggess D; Sundberg BA; Eilertsen K; Parimoo S; Filippi M; Stenn K. 2000. Asebia-2J (Scd1(ab2J)): a new allele and a model for scarring alopecia. Am J Pathol 156(6):2067-75. [PubMed: 10854228]  [MGI Ref ID J:62699]

Zheng Y; Eilertsen KJ; Ge L; Zhang L; Sundberg JP; Prouty SM; Stenn KS; Parimoo S. 1999. Scd1 is expressed in sebaceous glands and is disrupted in the asebia mouse [letter] Nat Genet 23(3):268-70. [PubMed: 10545940]  [MGI Ref ID J:58283]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThe homozygotes are very poor breeders and the mutation was best maintained using heterozygous females bred to homozygous males. It was also frozen using this breeding scheme.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery of Strains Needing Progeny Testing.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent.

    Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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General Terms and Conditions


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